P37980 (IPYR_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	PPA1
Synonyms:	PP

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Tissue specificity	Highest levels are found in retinal rod outer segments.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Inorganic pyrophosphatase		PRO_0000137566
Sites
	Metal binding	116	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 2 By similarity
	Metal binding	153	1	Magnesium 1 By similarity
Amino acid modifications
	Modified residue	57	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	3	1	G → S AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37980 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: 66E65A21F8C7892A	FASTA	289	32,814
	10 20 30 40 50 60 MSGFSSEERA APFTLEYRVF LKNEKGQYIS PFHDIPIYAD KEVFHMVVEV PRWSNAKMEI 70 80 90 100 110 120 ATKDPLNPIK QDVKKGKLRY VANLFPYKGY IWNYGAIPQT WEDPGHNDKH TGCCGDNDPI 130 140 150 160 170 180 DVCEIGSKVC ARGEIIRVKV LGILAMIDEG ETDWKVIAIN VEDPDAANYN DINDVKRLKP 190 200 210 220 230 240 GYLEATVDWF RRYKVPDGKP ENEFAFNAEF KDKNFAIDII ESTHDYWRAL VTKKTDGKGI 250 260 270 280 SCMNTTVSES PFQCDPDAAK AIVDALPPPC ESACTIPTDV DKWFHHQKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and functional expression of cDNA encoding a mammalian inorganic pyrophosphatase." Yang Z., Wensel T.G. J. Biol. Chem. 267:24641-24647(1992) [PubMed: 1339444] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Retina.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Ascending colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95283 mRNA. No translation available. BC114891 mRNA. Translation: AAI14892.1.
IPI	IPI00687219.
PIR	A45153.
RefSeq	NP_001068586.1. NM_001075118.1.
UniGene	Bt.7870.
3D structure databases
ProteinModelPortal	P37980.
SMR	P37980. Positions 4-285.
ModBase	Search...
Protein-protein interaction databases
STRING	P37980.
Proteomic databases
PRIDE	P37980.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000010311; ENSBTAP00000010311; ENSBTAG00000007836.
GeneID	280701.
KEGG	bta:280701.
Organism-specific databases
CTD	5464.
Phylogenomic databases
eggNOG	maNOG10459.
GeneTree	ENSGT00390000017004.
HOVERGEN	HBG000491.
InParanoid	P37980.
OrthoDB	EOG4KPTBC.
PhylomeDB	P37980.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_BOVIN

Accession

Primary (citable) accession number: P37980
Secondary accession number(s): Q1RMH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	April 3, 2007
Last modified:	November 16, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program