P37969 (GCP_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp

Alternative name(s):
t(6)A37 threonylcarbamoyladenosine biosynthesis protein

Gene names

Name:	gcp
Ordered Locus Names:	ML0379
ORF Names:	u229e, u1620c, B229_C3_246, B1620_C3_226

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine By similarity. HAMAP MF_01445
Subcellular location	Cytoplasm Potential HAMAP MF_01445.
Sequence similarities	Belongs to the KAE1 / YgjD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp HAMAP MF_01445		PRO_0000096969

Sequences

	Sequence		Length	Mass (Da)
	P37969 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: F76A14B739A7BAE4	FASTA	351	35,406
	10 20 30 40 50 60 MTISAVPGTI ILAIETSCDE TGVGIACLDD YGTVTLLADE VASSVDEQAR FGGVVPEIAS 70 80 90 100 110 120 RAHLEALGPT IRCALAAAGL TGSAKPDVVA ATIGPGLAGA LLVGVAAAKA YSAAWGVPFY 130 140 150 160 170 180 AVNHLGGHLA ADVYEHGPLP ECVALLVSGG HTHLLQVRSL GAPIVELGST VDDAAGEAYD 190 200 210 220 230 240 KVARLLGLGY PGGKVLDDLA RTGDRDAIVF PRGMTGPADD LNAFSFSGLK TAVARYVESH 250 260 270 280 290 300 PDALPADVAA GFQEAVADVL TMKAVRAATG LGVSTLLIVG GVAANSRLRE LAAQRCAAAG 310 320 330 340 350 LMLRIPGPRF CTDNGAMIAA FAAHLLAAAA PPSPLDVPSD PGLPVVKRQI N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.
[3]	"Mycobacteria contain two groEL genes: the second Mycobacterium leprae groEL gene is arranged in an operon with groES." de Wit T.F.R., Bekelie S., Osland A., Miko T.L., Hermans P.W.M., van Soolingen D., Drijfhout J., Schoeningh R., Janson A.A.M., Thole J.E.R. Mol. Microbiol. 6:1995-2007(1992) [PubMed: 1354834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 286-351.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00020 Genomic DNA. Translation: AAA17310.1. Sequence problems. AL583918 Genomic DNA. Translation: CAC29887.1. U00015 Genomic DNA. Translation: AAC43226.1.
PIR	S72817. S72996.
RefSeq	NP_301371.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P37969.
ModBase	Search...
Protein family/group databases
MEROPS	M22.A01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000029418; EBMYCP00000029024; EBMYCG00000029413.
GeneID	909071.
GenomeReviews	Gene locus ML0379 in contig AL450380_GR.
KEGG	mle:ML0379.
NMPDR	fig|272631.1.peg.243.
PATRIC	18051384. VBIMycLep78757_0641.
Organism-specific databases
Leproma	ML0379.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000017447.
HOGENOM	HBG304663.
OMA	PLYGVNH.
ProtClustDB	PRK09604.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0379-MONOMER.
Family and domain databases
HAMAP	MF_01445. gcp. [Tree]
InterPro	IPR022450. Pept_M22_O-sialoglycopept. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
KO	K01409.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR03723. Bact_gcp. 1 hit. TIGR00329. Gcp_kae1. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_MYCLE

Accession

Primary (citable) accession number: P37969
Secondary accession number(s): Q49725

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	July 15, 1998
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program