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P37967 (PNBA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Para-nitrobenzyl esterase

EC=3.1.1.-
Alternative name(s):
Intracellular esterase B
PNB carboxy-esterase
Short name=PNBCE
Gene names
Name:pnbA
Synonyms:estB
Ordered Locus Names:BSU34390
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes hydrolysis of several beta-lactam antibiotic PNB esters to the corresponding free acid and PNB alcohol.

Subunit structure

Monomer.

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 489489Para-nitrobenzyl esterase
PRO_0000070296

Sites

Active site1891Acyl-ester intermediate By similarity
Active site3101Charge relay system By similarity
Active site3991Charge relay system By similarity

Amino acid modifications

Modified residue1891Phosphoserine Ref.7

Natural variations

Natural variant591S → P in strain: NRRL B8079.
Natural variant951K → Q in strain: NRRL B8079.
Natural variant1501N → D in strain: NRRL B8079.
Natural variant2301S → A in strain: NRRL B8079.
Natural variant2421G → S in strain: NRRL B8079.
Natural variant2461K → R in strain: NRRL B8079.
Natural variant2511S → A in strain: NRRL B8079.
Natural variant2911A → S in strain: NRRL B8079.
Natural variant3431V → A in strain: NRRL B8079.
Natural variant3901K → E in strain: NRRL B8079.
Natural variant4631L → V in strain: NRRL B8079.

Experimental info

Sequence conflict171T → Q AA sequence Ref.1

Secondary structure

....................................................................................... 489
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P37967 [UniParc].

Last modified July 5, 2005. Version 2.
Checksum: 95D1EF12F613C400

FASTA48953,986
        10         20         30         40         50         60 
MTHQIVTTQY GKVKGTTENG VHKWKGIPYA KPPVGQWRFK APEPPEVWED VLDATAYGSI 

        70         80         90        100        110        120 
CPQPSDLLSL SYTELPRQSE DCLYVNVFAP DTPSKNLPVM VWIHGGAFYL GAGSEPLYDG 

       130        140        150        160        170        180 
SKLAAQGEVI VVTLNYRLGP FGFLHLSSFN EAYSDNLGLL DQAAALKWVR ENISAFGGDP 

       190        200        210        220        230        240 
DNVTVFGESA GGMSIAALLA MPAAKGLFQK AIMESGASRT MTKEQAASTS AAFLQVLGIN 

       250        260        270        280        290        300 
EGQLDKLHTV SAEDLLKAAD QLRIAEKENI FQLFFQPALD PKTLPEEPEK AIAEGAASGI 

       310        320        330        340        350        360 
PLLIGTTRDE GYLFFTPDSD VHSQETLDAA LEYLLGKPLA EKVADLYPRS LESQIHMMTD 

       370        380        390        400        410        420 
LLFWRPAVAY ASAQSHYAPV WMYRFDWHPK KPPYNKAFHA LELPFVFGNL DGLERMAKAE 

       430        440        450        460        470        480 
ITDEVKQLSH TIQSAWITFA KTGNPSTEAV NWPAYHEETR ETLILDSEIT IENDPESEKR 


QKLFPSKGE 

« Hide

References

« Hide 'large scale' references
[1]"The Bacillus subtilis pnbA gene encoding p-nitrobenzyl esterase: cloning, sequence and high-level expression in Escherichia coli."
Zock J., Cantwell C., Swartling J., Hodges R., Pohl T., Sutton K., Rosteck P. Jr., McGilvray D., Queener S.
Gene 151:37-43(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-22 AND 211-223.
Strain: NRRL B8079.
[2]"Identification of a SinR-homolog involved in initiation of competence development and sporulation in Bacillus subtilis."
Dartois V.A., Ferrari E., Hoch J.A.
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"Integrated mapping and sequencing of a 115 kb DNA fragment from Bacillus subtilis: sequence analysis of a 21 kb segment containing the sigL locus."
Fabret C., Quentin Y., Chapal N., Guiseppi A., Haiech J., Denizot F.
Microbiology 142:3089-3096(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[4]Denizot F.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[5]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[6]"Purification and properties of p-nitrobenzyl esterase from Bacillus subtilis."
Chen Y.-R., Usui S., Yu C.-A.
FASEB J. 6:A332-A332(1991)
Cited for: PROTEIN SEQUENCE OF 1-22 AND 211-223, CHARACTERIZATION.
[7]"The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis."
Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R., Mann M.
Mol. Cell. Proteomics 6:697-707(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: 168.
[8]"A structural view of evolutionary divergence."
Spiller B., Gershenson A., Arnold F.H., Stevens R.C.
Proc. Natl. Acad. Sci. U.S.A. 96:12305-12310(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U06089 Genomic DNA. Translation: AAA81915.1.
U46134 Genomic DNA. Translation: AAB39889.1.
Z71928 Genomic DNA. Translation: CAA96487.1.
Z94043 Genomic DNA. Translation: CAB08021.1.
AL009126 Genomic DNA. Translation: CAB15444.1.
PIRB69680.
RefSeqNP_391319.1. NC_000964.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1C7IX-ray2.00A1-489[»]
1C7JX-ray1.60A1-489[»]
1QE3X-ray1.50A1-489[»]
ProteinModelPortalP37967.
SMRP37967. Positions 2-486.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU34390.

Protein family/group databases

MEROPSS09.948.

PTM databases

PhosSiteP0802231.

Proteomic databases

PaxDbP37967.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15444; CAB15444; BSU34390.
GeneID938580.
KEGGbsu:BSU34390.
PATRIC18978878. VBIBacSub10457_3602.

Organism-specific databases

GenoListBSU34390. [Micado]

Phylogenomic databases

eggNOGCOG2272.
HOGENOMHOG000091866.
KOK03929.
OMAESAGACI.
OrthoDBEOG6X10Z4.
PhylomeDBP37967.

Enzyme and pathway databases

BioCycBSUB:BSU34390-MONOMER.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSPR00878. CHOLNESTRASE.
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP37967.

Entry information

Entry namePNBA_BACSU
AccessionPrimary (citable) accession number: P37967
Secondary accession number(s): O08167 expand/collapse secondary AC list , O08472, P71048, Q795H9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 5, 2005
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList