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Protein

Para-nitrobenzyl esterase

Gene

pnbA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes hydrolysis of several beta-lactam antibiotic PNB esters to the corresponding free acid and PNB alcohol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei189Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei310Charge relay systemBy similarity1
Active sitei399Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BioCyciBSUB:BSU34390-MONOMER.
BRENDAi3.1.1.1. 658.

Protein family/group databases

ESTHERibacsu-pnbae. Carb_B_Bacteria.
MEROPSiS09.948.

Names & Taxonomyi

Protein namesi
Recommended name:
Para-nitrobenzyl esterase (EC:3.1.1.-)
Alternative name(s):
Intracellular esterase B
PNB carboxy-esterase
Short name:
PNBCE
Gene namesi
Name:pnbA
Synonyms:estB
Ordered Locus Names:BSU34390
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000702961 – 489Para-nitrobenzyl esteraseAdd BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei189Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP37967.

PTM databases

iPTMnetiP37967.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018636.

Structurei

Secondary structure

1489
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 8Combined sources4
Beta strandi11 – 14Combined sources4
Beta strandi16 – 18Combined sources3
Beta strandi21 – 28Combined sources8
Helixi35 – 37Combined sources3
Beta strandi49 – 53Combined sources5
Turni65 – 67Combined sources3
Helixi68 – 71Combined sources4
Beta strandi84 – 90Combined sources7
Beta strandi96 – 103Combined sources8
Turni107 – 109Combined sources3
Helixi116 – 118Combined sources3
Helixi121 – 127Combined sources7
Beta strandi130 – 134Combined sources5
Helixi139 – 142Combined sources4
Turni147 – 149Combined sources3
Helixi157 – 172Combined sources16
Helixi173 – 176Combined sources4
Beta strandi178 – 188Combined sources11
Helixi190 – 198Combined sources9
Helixi202 – 204Combined sources3
Beta strandi209 – 215Combined sources7
Helixi223 – 237Combined sources15
Helixi244 – 248Combined sources5
Helixi252 – 263Combined sources12
Beta strandi274 – 276Combined sources3
Turni281 – 283Combined sources3
Helixi288 – 293Combined sources6
Turni294 – 299Combined sources6
Beta strandi302 – 307Combined sources6
Helixi310 – 313Combined sources4
Helixi324 – 335Combined sources12
Helixi337 – 343Combined sources7
Helixi344 – 346Combined sources3
Helixi351 – 362Combined sources12
Helixi364 – 374Combined sources11
Turni375 – 377Combined sources3
Beta strandi380 – 385Combined sources6
Turni399 – 402Combined sources4
Helixi403 – 407Combined sources5
Helixi412 – 417Combined sources6
Helixi423 – 442Combined sources20
Turni457 – 459Combined sources3
Beta strandi461 – 468Combined sources8
Beta strandi470 – 474Combined sources5
Helixi477 – 483Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C7IX-ray2.00A1-489[»]
1C7JX-ray1.60A1-489[»]
1QE3X-ray1.50A1-489[»]
ProteinModelPortaliP37967.
SMRiP37967.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37967.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EBR. Bacteria.
COG2272. LUCA.
HOGENOMiHOG000091866.
InParanoidiP37967.
KOiK03929.
OMAiSAGACIT.
PhylomeDBiP37967.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37967-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHQIVTTQY GKVKGTTENG VHKWKGIPYA KPPVGQWRFK APEPPEVWED
60 70 80 90 100
VLDATAYGSI CPQPSDLLSL SYTELPRQSE DCLYVNVFAP DTPSKNLPVM
110 120 130 140 150
VWIHGGAFYL GAGSEPLYDG SKLAAQGEVI VVTLNYRLGP FGFLHLSSFN
160 170 180 190 200
EAYSDNLGLL DQAAALKWVR ENISAFGGDP DNVTVFGESA GGMSIAALLA
210 220 230 240 250
MPAAKGLFQK AIMESGASRT MTKEQAASTS AAFLQVLGIN EGQLDKLHTV
260 270 280 290 300
SAEDLLKAAD QLRIAEKENI FQLFFQPALD PKTLPEEPEK AIAEGAASGI
310 320 330 340 350
PLLIGTTRDE GYLFFTPDSD VHSQETLDAA LEYLLGKPLA EKVADLYPRS
360 370 380 390 400
LESQIHMMTD LLFWRPAVAY ASAQSHYAPV WMYRFDWHPK KPPYNKAFHA
410 420 430 440 450
LELPFVFGNL DGLERMAKAE ITDEVKQLSH TIQSAWITFA KTGNPSTEAV
460 470 480
NWPAYHEETR ETLILDSEIT IENDPESEKR QKLFPSKGE
Length:489
Mass (Da):53,986
Last modified:July 5, 2005 - v2
Checksum:i95D1EF12F613C400
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17T → Q AA sequence (PubMed:7828905).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti59S → P in strain: NRRL B8079. 1
Natural varianti95K → Q in strain: NRRL B8079. 1
Natural varianti150N → D in strain: NRRL B8079. 1
Natural varianti230S → A in strain: NRRL B8079. 1
Natural varianti242G → S in strain: NRRL B8079. 1
Natural varianti246K → R in strain: NRRL B8079. 1
Natural varianti251S → A in strain: NRRL B8079. 1
Natural varianti291A → S in strain: NRRL B8079. 1
Natural varianti343V → A in strain: NRRL B8079. 1
Natural varianti390K → E in strain: NRRL B8079. 1
Natural varianti463L → V in strain: NRRL B8079. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06089 Genomic DNA. Translation: AAA81915.1.
U46134 Genomic DNA. Translation: AAB39889.1.
Z71928 Genomic DNA. Translation: CAA96487.1.
Z94043 Genomic DNA. Translation: CAB08021.1.
AL009126 Genomic DNA. Translation: CAB15444.1.
PIRiB69680.
RefSeqiNP_391319.1. NC_000964.3.
WP_003243926.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15444; CAB15444; BSU34390.
GeneIDi938580.
KEGGibsu:BSU34390.
PATRICi18978878. VBIBacSub10457_3602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06089 Genomic DNA. Translation: AAA81915.1.
U46134 Genomic DNA. Translation: AAB39889.1.
Z71928 Genomic DNA. Translation: CAA96487.1.
Z94043 Genomic DNA. Translation: CAB08021.1.
AL009126 Genomic DNA. Translation: CAB15444.1.
PIRiB69680.
RefSeqiNP_391319.1. NC_000964.3.
WP_003243926.1. NZ_JNCM01000033.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C7IX-ray2.00A1-489[»]
1C7JX-ray1.60A1-489[»]
1QE3X-ray1.50A1-489[»]
ProteinModelPortaliP37967.
SMRiP37967.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018636.

Protein family/group databases

ESTHERibacsu-pnbae. Carb_B_Bacteria.
MEROPSiS09.948.

PTM databases

iPTMnetiP37967.

Proteomic databases

PaxDbiP37967.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15444; CAB15444; BSU34390.
GeneIDi938580.
KEGGibsu:BSU34390.
PATRICi18978878. VBIBacSub10457_3602.

Phylogenomic databases

eggNOGiENOG4105EBR. Bacteria.
COG2272. LUCA.
HOGENOMiHOG000091866.
InParanoidiP37967.
KOiK03929.
OMAiSAGACIT.
PhylomeDBiP37967.

Enzyme and pathway databases

BioCyciBSUB:BSU34390-MONOMER.
BRENDAi3.1.1.1. 658.

Miscellaneous databases

EvolutionaryTraceiP37967.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNBA_BACSU
AccessioniPrimary (citable) accession number: P37967
Secondary accession number(s): O08167
, O08472, P71048, Q795H9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 5, 2005
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.