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Protein

UvrABC system protein B

Gene

uvrB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU35170-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Protein DinA
Gene namesi
Name:uvrBUniRule annotation
Synonyms:dinA, uvr
Ordered Locus Names:BSU35170
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001383811 – 661UvrABC system protein BAdd BLAST661

Proteomic databases

PaxDbiP37954.
PRIDEiP37954.

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019031.

Structurei

Secondary structure

1661
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 29Combined sources13
Beta strandi33 – 39Combined sources7
Helixi45 – 56Combined sources12
Beta strandi60 – 63Combined sources4
Helixi67 – 80Combined sources14
Beta strandi84 – 89Combined sources6
Beta strandi93 – 97Combined sources5
Beta strandi100 – 102Combined sources3
Turni103 – 106Combined sources4
Beta strandi107 – 109Combined sources3
Beta strandi112 – 115Combined sources4
Helixi117 – 132Combined sources16
Beta strandi136 – 140Combined sources5
Helixi142 – 145Combined sources4
Helixi151 – 157Combined sources7
Beta strandi159 – 162Combined sources4
Helixi169 – 178Combined sources10
Beta strandi185 – 187Combined sources3
Beta strandi192 – 196Combined sources5
Beta strandi199 – 203Combined sources5
Beta strandi208 – 228Combined sources21
Turni229 – 231Combined sources3
Beta strandi234 – 237Combined sources4
Beta strandi239 – 243Combined sources5
Beta strandi246 – 249Combined sources4
Helixi253 – 276Combined sources24
Helixi280 – 300Combined sources21
Helixi306 – 309Combined sources4
Helixi310 – 313Combined sources4
Helixi325 – 328Combined sources4
Beta strandi334 – 338Combined sources5
Helixi340 – 363Combined sources24
Helixi369 – 373Combined sources5
Helixi379 – 384Combined sources6
Beta strandi387 – 392Combined sources6
Helixi398 – 403Combined sources6
Beta strandi408 – 410Combined sources3
Beta strandi421 – 425Combined sources5
Helixi430 – 442Combined sources13
Turni443 – 445Combined sources3
Beta strandi447 – 451Combined sources5
Helixi455 – 467Combined sources13
Beta strandi472 – 475Combined sources4
Helixi481 – 492Combined sources12
Beta strandi497 – 502Combined sources6
Beta strandi514 – 519Combined sources6
Turni520 – 523Combined sources4
Turni526 – 529Combined sources4
Helixi531 – 539Combined sources9
Turni540 – 543Combined sources4
Beta strandi548 – 552Combined sources5
Helixi558 – 581Combined sources24
Helixi622 – 640Combined sources19
Helixi644 – 653Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D7DX-ray2.10A1-661[»]
B622-661[»]
2NMVX-ray2.95A1-661[»]
B622-659[»]
3V4RX-ray3.25A/B1-661[»]
ProteinModelPortaliP37954.
SMRiP37954.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37954.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 413Helicase ATP-bindingUniRule annotationAdd BLAST388
Domaini430 – 596Helicase C-terminalUniRule annotationAdd BLAST167
Domaini625 – 660UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi92 – 115Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation
Contains 1 helicase C-terminal domain.UniRule annotation
Contains 1 UVR domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
InParanoidiP37954.
KOiK03702.
OMAiQEYVDRM.
PhylomeDBiP37954.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDRFELVSK YQPQGDQPKA IEKLVKGIQE GKKHQTLLGA TGTGKTFTVS
60 70 80 90 100
NLIKEVNKPT LVIAHNKTLA GQLYSEFKEF FPNNAVEYFV SYYDYYQPEA
110 120 130 140 150
YVPQTDTFIE KDASINDEID KLRHSATSAL FERRDVIIIA SVSCIYGLGS
160 170 180 190 200
PEEYREMVVS LRTEMEIERN ELLRKLVDIQ YARNDIDFQR GTFRVRGDVV
210 220 230 240 250
EIFPASRDEH CVRVEFFGDE IERIREVDAL TGEILGDRDH VAIFPASHFV
260 270 280 290 300
TRAEKMEKAI QNIEKELEEQ LKVMHENGKL LEAQRLEQRT RYDLEMMREM
310 320 330 340 350
GFCSGIENYS RHLTLRPPGS TPYTLLDYFP DDFMIVVDES HVTIPQVRGM
360 370 380 390 400
FNGDQARKQV LVDHGFRLPS ALDNRPLRFE EFEKHMHNIV YVSATPGPYE
410 420 430 440 450
IEHTDEMVEQ IIRPTGLLDP LIDVRPIEGQ IDDLIGEIQA RIERNERVLV
460 470 480 490 500
TTLTKKMSED LTDYLKEIGI KVNYLHSEIK TLERIEIIRD LRLGKYDVLV
510 520 530 540 550
GINLLREGLD IPEVSLVAIL DADKEGFLRS ERSLIQTIGR AARNAEGRVI
560 570 580 590 600
MYADKITKSM EIAINETKRR REQQERFNEE HGITPKTINK EIRDVIRATV
610 620 630 640 650
AAEDKAEYKT KAAPKLSKMT KKERQKVVEQ MEHEMKEAAK ALDFERAAEL
660
RDLLLELKAE G
Length:661
Mass (Da):76,327
Last modified:July 15, 1998 - v2
Checksum:iA3C0A862A87F867C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178D → A in AAA22360 (PubMed:1744042).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA. Translation: AAC67270.1.
AL009126 Genomic DNA. Translation: CAB15534.1.
M80473 Genomic DNA. Translation: AAA22360.1.
M64048 Genomic DNA. Translation: AAA22389.1.
PIRiG69729.
RefSeqiNP_391397.1. NC_000964.3.
WP_003243787.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15534; CAB15534; BSU35170.
GeneIDi936663.
KEGGibsu:BSU35170.
PATRICi18979040. VBIBacSub10457_3682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA. Translation: AAC67270.1.
AL009126 Genomic DNA. Translation: CAB15534.1.
M80473 Genomic DNA. Translation: AAA22360.1.
M64048 Genomic DNA. Translation: AAA22389.1.
PIRiG69729.
RefSeqiNP_391397.1. NC_000964.3.
WP_003243787.1. NZ_JNCM01000033.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D7DX-ray2.10A1-661[»]
B622-661[»]
2NMVX-ray2.95A1-661[»]
B622-659[»]
3V4RX-ray3.25A/B1-661[»]
ProteinModelPortaliP37954.
SMRiP37954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019031.

Proteomic databases

PaxDbiP37954.
PRIDEiP37954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15534; CAB15534; BSU35170.
GeneIDi936663.
KEGGibsu:BSU35170.
PATRICi18979040. VBIBacSub10457_3682.

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
InParanoidiP37954.
KOiK03702.
OMAiQEYVDRM.
PhylomeDBiP37954.

Enzyme and pathway databases

BioCyciBSUB:BSU35170-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37954.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUVRB_BACSU
AccessioniPrimary (citable) accession number: P37954
Secondary accession number(s): O34455
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.