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Protein

UvrABC system protein B

Gene

uvrB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU35170-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Protein DinA
Gene namesi
Name:uvrBUniRule annotation
Synonyms:dinA, uvr
Ordered Locus Names:BSU35170
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001383811 – 661UvrABC system protein BAdd BLAST661

Proteomic databases

PaxDbiP37954
PRIDEiP37954

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019031

Structurei

Secondary structure

1661
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 29Combined sources13
Beta strandi33 – 39Combined sources7
Helixi45 – 56Combined sources12
Beta strandi60 – 63Combined sources4
Helixi67 – 80Combined sources14
Beta strandi84 – 89Combined sources6
Beta strandi93 – 97Combined sources5
Beta strandi100 – 102Combined sources3
Turni103 – 106Combined sources4
Beta strandi107 – 109Combined sources3
Beta strandi112 – 115Combined sources4
Helixi117 – 132Combined sources16
Beta strandi136 – 140Combined sources5
Helixi142 – 145Combined sources4
Helixi151 – 157Combined sources7
Beta strandi159 – 162Combined sources4
Helixi169 – 178Combined sources10
Beta strandi185 – 187Combined sources3
Beta strandi192 – 196Combined sources5
Beta strandi199 – 203Combined sources5
Beta strandi208 – 228Combined sources21
Turni229 – 231Combined sources3
Beta strandi234 – 237Combined sources4
Beta strandi239 – 243Combined sources5
Beta strandi246 – 249Combined sources4
Helixi253 – 276Combined sources24
Helixi280 – 300Combined sources21
Helixi306 – 309Combined sources4
Helixi310 – 313Combined sources4
Helixi325 – 328Combined sources4
Beta strandi334 – 338Combined sources5
Helixi340 – 363Combined sources24
Helixi369 – 373Combined sources5
Helixi379 – 384Combined sources6
Beta strandi387 – 392Combined sources6
Helixi398 – 403Combined sources6
Beta strandi408 – 410Combined sources3
Beta strandi421 – 425Combined sources5
Helixi430 – 442Combined sources13
Turni443 – 445Combined sources3
Beta strandi447 – 451Combined sources5
Helixi455 – 467Combined sources13
Beta strandi472 – 475Combined sources4
Helixi481 – 492Combined sources12
Beta strandi497 – 502Combined sources6
Beta strandi514 – 519Combined sources6
Turni520 – 523Combined sources4
Turni526 – 529Combined sources4
Helixi531 – 539Combined sources9
Turni540 – 543Combined sources4
Beta strandi548 – 552Combined sources5
Helixi558 – 581Combined sources24
Helixi622 – 640Combined sources19
Helixi644 – 653Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D7DX-ray2.10A1-661[»]
B622-661[»]
2NMVX-ray2.95A1-661[»]
B622-659[»]
3V4RX-ray3.25A/B1-661[»]
ProteinModelPortaliP37954
SMRiP37954
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37954

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 413Helicase ATP-bindingUniRule annotationAdd BLAST388
Domaini430 – 596Helicase C-terminalUniRule annotationAdd BLAST167
Domaini625 – 660UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi92 – 115Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCW Bacteria
COG0556 LUCA
HOGENOMiHOG000073580
InParanoidiP37954
KOiK03702
OMAiRYMHSEI
PhylomeDBiP37954

Family and domain databases

CDDicd00079 HELICc, 1 hit
HAMAPiMF_00204 UvrB, 1 hit
InterProiView protein in InterPro
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR001943 UVR_dom
IPR036876 UVR_dom_sf
IPR004807 UvrB
IPR024759 UvrB_YAD/RRR_dom
PANTHERiPTHR24029 PTHR24029, 1 hit
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF04851 ResIII, 1 hit
PF02151 UVR, 1 hit
PF12344 UvrB, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF46600 SSF46600, 1 hit
SSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00631 uvrb, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50151 UVR, 1 hit

Sequencei

Sequence statusi: Complete.

P37954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDRFELVSK YQPQGDQPKA IEKLVKGIQE GKKHQTLLGA TGTGKTFTVS
60 70 80 90 100
NLIKEVNKPT LVIAHNKTLA GQLYSEFKEF FPNNAVEYFV SYYDYYQPEA
110 120 130 140 150
YVPQTDTFIE KDASINDEID KLRHSATSAL FERRDVIIIA SVSCIYGLGS
160 170 180 190 200
PEEYREMVVS LRTEMEIERN ELLRKLVDIQ YARNDIDFQR GTFRVRGDVV
210 220 230 240 250
EIFPASRDEH CVRVEFFGDE IERIREVDAL TGEILGDRDH VAIFPASHFV
260 270 280 290 300
TRAEKMEKAI QNIEKELEEQ LKVMHENGKL LEAQRLEQRT RYDLEMMREM
310 320 330 340 350
GFCSGIENYS RHLTLRPPGS TPYTLLDYFP DDFMIVVDES HVTIPQVRGM
360 370 380 390 400
FNGDQARKQV LVDHGFRLPS ALDNRPLRFE EFEKHMHNIV YVSATPGPYE
410 420 430 440 450
IEHTDEMVEQ IIRPTGLLDP LIDVRPIEGQ IDDLIGEIQA RIERNERVLV
460 470 480 490 500
TTLTKKMSED LTDYLKEIGI KVNYLHSEIK TLERIEIIRD LRLGKYDVLV
510 520 530 540 550
GINLLREGLD IPEVSLVAIL DADKEGFLRS ERSLIQTIGR AARNAEGRVI
560 570 580 590 600
MYADKITKSM EIAINETKRR REQQERFNEE HGITPKTINK EIRDVIRATV
610 620 630 640 650
AAEDKAEYKT KAAPKLSKMT KKERQKVVEQ MEHEMKEAAK ALDFERAAEL
660
RDLLLELKAE G
Length:661
Mass (Da):76,327
Last modified:July 15, 1998 - v2
Checksum:iA3C0A862A87F867C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178D → A in AAA22360 (PubMed:1744042).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA Translation: AAC67270.1
AL009126 Genomic DNA Translation: CAB15534.1
M80473 Genomic DNA Translation: AAA22360.1
M64048 Genomic DNA Translation: AAA22389.1
PIRiG69729
RefSeqiNP_391397.1, NC_000964.3
WP_003243787.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15534; CAB15534; BSU35170
GeneIDi936663
KEGGibsu:BSU35170
PATRICifig|224308.179.peg.3807

Similar proteinsi

Entry informationi

Entry nameiUVRB_BACSU
AccessioniPrimary (citable) accession number: P37954
Secondary accession number(s): O34455
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 15, 1998
Last modified: May 23, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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