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Protein

UvrABC system protein B

Gene

uvrB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 468ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU35170-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Protein DinA
Gene namesi
Name:uvrBUniRule annotation
Synonyms:dinA, uvr
Ordered Locus Names:BSU35170
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 661661UvrABC system protein BPRO_0000138381Add
BLAST

Proteomic databases

PaxDbiP37954.

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019031.

Structurei

Secondary structure

1
661
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi17 – 2913Combined sources
Beta strandi33 – 397Combined sources
Helixi45 – 5612Combined sources
Beta strandi60 – 634Combined sources
Helixi67 – 8014Combined sources
Beta strandi84 – 896Combined sources
Beta strandi93 – 975Combined sources
Beta strandi100 – 1023Combined sources
Turni103 – 1064Combined sources
Beta strandi107 – 1093Combined sources
Beta strandi112 – 1154Combined sources
Helixi117 – 13216Combined sources
Beta strandi136 – 1405Combined sources
Helixi142 – 1454Combined sources
Helixi151 – 1577Combined sources
Beta strandi159 – 1624Combined sources
Helixi169 – 17810Combined sources
Beta strandi185 – 1873Combined sources
Beta strandi192 – 1965Combined sources
Beta strandi199 – 2035Combined sources
Beta strandi208 – 22821Combined sources
Turni229 – 2313Combined sources
Beta strandi234 – 2374Combined sources
Beta strandi239 – 2435Combined sources
Beta strandi246 – 2494Combined sources
Helixi253 – 27624Combined sources
Helixi280 – 30021Combined sources
Helixi306 – 3094Combined sources
Helixi310 – 3134Combined sources
Helixi325 – 3284Combined sources
Beta strandi334 – 3385Combined sources
Helixi340 – 36324Combined sources
Helixi369 – 3735Combined sources
Helixi379 – 3846Combined sources
Beta strandi387 – 3926Combined sources
Helixi398 – 4036Combined sources
Beta strandi408 – 4103Combined sources
Beta strandi421 – 4255Combined sources
Helixi430 – 44213Combined sources
Turni443 – 4453Combined sources
Beta strandi447 – 4515Combined sources
Helixi455 – 46713Combined sources
Beta strandi472 – 4754Combined sources
Helixi481 – 49212Combined sources
Beta strandi497 – 5026Combined sources
Beta strandi514 – 5196Combined sources
Turni520 – 5234Combined sources
Turni526 – 5294Combined sources
Helixi531 – 5399Combined sources
Turni540 – 5434Combined sources
Beta strandi548 – 5525Combined sources
Helixi558 – 58124Combined sources
Helixi622 – 64019Combined sources
Helixi644 – 65310Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D7DX-ray2.10A1-661[»]
B622-661[»]
2NMVX-ray2.95A1-661[»]
B622-659[»]
3V4RX-ray3.25A/B1-661[»]
ProteinModelPortaliP37954.
SMRiP37954. Positions 3-659.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37954.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 413388Helicase ATP-bindingUniRule annotationAdd
BLAST
Domaini430 – 596167Helicase C-terminalUniRule annotationAdd
BLAST
Domaini625 – 66036UVRUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi92 – 11524Beta-hairpinAdd
BLAST

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation
Contains 1 helicase C-terminal domain.UniRule annotation
Contains 1 UVR domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
InParanoidiP37954.
KOiK03702.
OMAiQEYVDRM.
OrthoDBiEOG6B360R.
PhylomeDBiP37954.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDRFELVSK YQPQGDQPKA IEKLVKGIQE GKKHQTLLGA TGTGKTFTVS
60 70 80 90 100
NLIKEVNKPT LVIAHNKTLA GQLYSEFKEF FPNNAVEYFV SYYDYYQPEA
110 120 130 140 150
YVPQTDTFIE KDASINDEID KLRHSATSAL FERRDVIIIA SVSCIYGLGS
160 170 180 190 200
PEEYREMVVS LRTEMEIERN ELLRKLVDIQ YARNDIDFQR GTFRVRGDVV
210 220 230 240 250
EIFPASRDEH CVRVEFFGDE IERIREVDAL TGEILGDRDH VAIFPASHFV
260 270 280 290 300
TRAEKMEKAI QNIEKELEEQ LKVMHENGKL LEAQRLEQRT RYDLEMMREM
310 320 330 340 350
GFCSGIENYS RHLTLRPPGS TPYTLLDYFP DDFMIVVDES HVTIPQVRGM
360 370 380 390 400
FNGDQARKQV LVDHGFRLPS ALDNRPLRFE EFEKHMHNIV YVSATPGPYE
410 420 430 440 450
IEHTDEMVEQ IIRPTGLLDP LIDVRPIEGQ IDDLIGEIQA RIERNERVLV
460 470 480 490 500
TTLTKKMSED LTDYLKEIGI KVNYLHSEIK TLERIEIIRD LRLGKYDVLV
510 520 530 540 550
GINLLREGLD IPEVSLVAIL DADKEGFLRS ERSLIQTIGR AARNAEGRVI
560 570 580 590 600
MYADKITKSM EIAINETKRR REQQERFNEE HGITPKTINK EIRDVIRATV
610 620 630 640 650
AAEDKAEYKT KAAPKLSKMT KKERQKVVEQ MEHEMKEAAK ALDFERAAEL
660
RDLLLELKAE G
Length:661
Mass (Da):76,327
Last modified:July 15, 1998 - v2
Checksum:iA3C0A862A87F867C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781D → A in AAA22360 (PubMed:1744042).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA. Translation: AAC67270.1.
AL009126 Genomic DNA. Translation: CAB15534.1.
M80473 Genomic DNA. Translation: AAA22360.1.
M64048 Genomic DNA. Translation: AAA22389.1.
PIRiG69729.
RefSeqiNP_391397.1. NC_000964.3.
WP_003243787.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15534; CAB15534; BSU35170.
GeneIDi936663.
KEGGibsu:BSU35170.
PATRICi18979040. VBIBacSub10457_3682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA. Translation: AAC67270.1.
AL009126 Genomic DNA. Translation: CAB15534.1.
M80473 Genomic DNA. Translation: AAA22360.1.
M64048 Genomic DNA. Translation: AAA22389.1.
PIRiG69729.
RefSeqiNP_391397.1. NC_000964.3.
WP_003243787.1. NZ_JNCM01000033.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D7DX-ray2.10A1-661[»]
B622-661[»]
2NMVX-ray2.95A1-661[»]
B622-659[»]
3V4RX-ray3.25A/B1-661[»]
ProteinModelPortaliP37954.
SMRiP37954. Positions 3-659.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019031.

Proteomic databases

PaxDbiP37954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15534; CAB15534; BSU35170.
GeneIDi936663.
KEGGibsu:BSU35170.
PATRICi18979040. VBIBacSub10457_3682.

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
InParanoidiP37954.
KOiK03702.
OMAiQEYVDRM.
OrthoDBiEOG6B360R.
PhylomeDBiP37954.

Enzyme and pathway databases

BioCyciBSUB:BSU35170-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37954.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the 300-304 chromosomal segment of Bacillus subtilis."
    Lazarevic V., Soldo B., Rivolta C., Reynolds S., Mauel C., Karamata D.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Genetic method to identify regulons controlled by nonessential elements: isolation of a gene dependent on alternate transcription factor sigma B of Bacillus subtilis."
    Boylan S.A., Thomas M.D., Price C.W.
    J. Bacteriol. 173:7856-7866(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-186.
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  4. "Cloning and characterization of DNA damage-inducible promoter regions from Bacillus subtilis."
    Cheo D.L., Bayles K.W., Yasbin R.E.
    J. Bacteriol. 173:1696-1703(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-57.

Entry informationi

Entry nameiUVRB_BACSU
AccessioniPrimary (citable) accession number: P37954
Secondary accession number(s): O34455
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 15, 1998
Last modified: February 17, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.