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Protein

Lon protease 1

Gene

lon1

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner (By similarity). Has been implicated in preventing sigma(G) activity under non-sporulation conditions.UniRule annotation

Catalytic activityi

Hydrolysis of proteins in presence of ATP.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei677UniRule annotation1
Active sitei720UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi354 – 361ATP8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU28200-MONOMER.
BRENDAi3.4.21.53. 658.

Protein family/group databases

MEROPSiS16.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Lon protease 1UniRule annotation (EC:3.4.21.53UniRule annotation)
Alternative name(s):
ATP-dependent protease La 1UniRule annotation
Gene namesi
Name:lon1UniRule annotation
Synonyms:lonA
Ordered Locus Names:BSU28200
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000761161 – 774Lon protease 1Add BLAST774

Proteomic databases

PaxDbiP37945.
PRIDEiP37945.

Expressioni

Inductioni

By heat shock.UniRule annotation1 Publication

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity (By similarity). Exists as a mixture of small oligomeric species in solution.UniRule annotation1 Publication

Protein-protein interaction databases

IntActiP37945. 2 interactors.
MINTiMINT-8366587.
STRINGi224308.Bsubs1_010100015406.

Structurei

Secondary structure

1774
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 16Combined sources12
Beta strandi24 – 29Combined sources6
Helixi32 – 41Combined sources10
Turni42 – 45Combined sources4
Beta strandi46 – 55Combined sources10
Helixi63 – 65Combined sources3
Beta strandi68 – 80Combined sources13
Beta strandi86 – 103Combined sources18
Beta strandi105 – 114Combined sources10
Helixi124 – 155Combined sources32
Helixi160 – 170Combined sources11
Helixi175 – 182Combined sources8
Helixi187 – 207Combined sources21
Turni248 – 250Combined sources3
Helixi251 – 257Combined sources7
Beta strandi258 – 260Combined sources3
Helixi264 – 274Combined sources11
Beta strandi280 – 282Combined sources3
Turni284 – 287Combined sources4
Helixi288 – 298Combined sources11
Turni311 – 313Combined sources3
Helixi314 – 316Combined sources3
Helixi318 – 321Combined sources4
Helixi326 – 341Combined sources16
Beta strandi342 – 344Combined sources3
Beta strandi349 – 359Combined sources11
Helixi360 – 371Combined sources12
Beta strandi374 – 378Combined sources5
Helixi404 – 409Combined sources6
Beta strandi413 – 425Combined sources13
Helixi439 – 441Combined sources3
Turni444 – 448Combined sources5
Beta strandi453 – 455Combined sources3
Beta strandi465 – 469Combined sources5
Turni473 – 475Combined sources3
Helixi478 – 483Combined sources6
Beta strandi484 – 488Combined sources5
Helixi494 – 503Combined sources10
Helixi505 – 512Combined sources8
Helixi517 – 519Combined sources3
Helixi524 – 534Combined sources11
Beta strandi538 – 540Combined sources3
Helixi541 – 559Combined sources19
Beta strandi560 – 562Combined sources3
Turni570 – 573Combined sources4
Helixi574 – 577Combined sources4
Turni586 – 588Combined sources3
Beta strandi592 – 602Combined sources11
Beta strandi605 – 618Combined sources14
Beta strandi622 – 627Combined sources6
Helixi630 – 643Combined sources14
Helixi648 – 650Combined sources3
Turni657 – 659Combined sources3
Beta strandi661 – 666Combined sources6
Helixi672 – 678Combined sources7
Helixi679 – 690Combined sources12
Beta strandi710 – 712Combined sources3
Helixi717 – 726Combined sources10
Beta strandi730 – 735Combined sources6
Helixi736 – 744Combined sources9
Helixi747 – 750Combined sources4
Beta strandi757 – 760Combined sources4
Helixi761 – 768Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X37NMR-A490-604[»]
3M65X-ray2.60A/B1-209[»]
3M6AX-ray3.40A/B/C/D/E/F240-774[»]
ProteinModelPortaliP37945.
SMRiP37945.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37945.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 200Lon N-terminalPROSITE-ProRule annotationAdd BLAST192
Domaini590 – 771Lon proteolyticPROSITE-ProRule annotationAdd BLAST182

Sequence similaritiesi

Belongs to the peptidase S16 family.UniRule annotation
Contains 1 Lon N-terminal domain.PROSITE-ProRule annotation
Contains 1 Lon proteolytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
HOGENOMiHOG000261410.
InParanoidiP37945.
KOiK01338.
OMAiVNIAQRY.
PhylomeDBiP37945.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37945-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEELKRSIP LLPLRGLLVY PTMVLHLDVG RDKSVQALEQ AMMHDHMIFL
60 70 80 90 100
ATQQDISIDE PGEDEIFTVG TYTKIKQMLK LPNGTIRVLV EGLKRAHIVK
110 120 130 140 150
YNEHEDYTSV DIQLIHEDDS KDTEDEALMR TLLDHFDQYI KISKKISAET
160 170 180 190 200
YAAVTDIEEP GRMADIVASH LPLKLKDKQD ILETADVKDR LNKVIDFINN
210 220 230 240 250
EKEVLEIEKK IGQRVKRSME RTQKEYYLRE QMKAIQKELG DKEGKTGEVQ
260 270 280 290 300
TLTEKIEEAG MPDHVKETAL KELNRYEKIP SSSAESSVIR NYIDWLVALP
310 320 330 340 350
WTDETDDKLD LKEAGRLLDE EHHGLEKVKE RILEYLAVQK LTKSLKGPIL
360 370 380 390 400
CLAGPPGVGK TSLAKSIAKS LGRKFVRISL GGVRDESEIR GHRRTYVGAM
410 420 430 440 450
PGRIIQGMKK AGKLNPVFLL DEIDKMSSDF RGDPSSAMLE VLDPEQNSSF
460 470 480 490 500
SDHYIEETFD LSKVLFIATA NNLATIPGPL RDRMEIINIA GYTEIEKLEI
510 520 530 540 550
VKDHLLPKQI KEHGLKKSNL QLRDQAILDI IRYYTREAGV RSLERQLAAI
560 570 580 590 600
CRKAAKAIVA EERKRITVTE KNLQDFIGKR IFRYGQAETE DQVGVVTGLA
610 620 630 640 650
YTTVGGDTLS IEVSLSPGKG KLILTGKLGD VMRESAQAAF SYVRSKTEEL
660 670 680 690 700
GIEPDFHEKY DIHIHVPEGA VPKDGPSAGI TMATALVSAL TGRAVSREVG
710 720 730 740 750
MTGEITLRGR VLPIGGLKEK ALGAHRAGLT TIIAPKDNEK DIEDIPESVR
760 770
EGLTFILASH LDEVLEHALV GEKK
Length:774
Mass (Da):86,607
Last modified:October 1, 1994 - v1
Checksum:iBE339D896FCEF533
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76424 Genomic DNA. Translation: CAA53984.1.
Z75208 Genomic DNA. Translation: CAA99540.1.
AL009126 Genomic DNA. Translation: CAB14780.1.
PIRiI40421.
RefSeqiNP_390698.1. NC_000964.3.
WP_003229618.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14780; CAB14780; BSU28200.
GeneIDi937486.
KEGGibsu:BSU28200.
PATRICi18977516. VBIBacSub10457_2947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76424 Genomic DNA. Translation: CAA53984.1.
Z75208 Genomic DNA. Translation: CAA99540.1.
AL009126 Genomic DNA. Translation: CAB14780.1.
PIRiI40421.
RefSeqiNP_390698.1. NC_000964.3.
WP_003229618.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X37NMR-A490-604[»]
3M65X-ray2.60A/B1-209[»]
3M6AX-ray3.40A/B/C/D/E/F240-774[»]
ProteinModelPortaliP37945.
SMRiP37945.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP37945. 2 interactors.
MINTiMINT-8366587.
STRINGi224308.Bsubs1_010100015406.

Protein family/group databases

MEROPSiS16.001.

Proteomic databases

PaxDbiP37945.
PRIDEiP37945.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14780; CAB14780; BSU28200.
GeneIDi937486.
KEGGibsu:BSU28200.
PATRICi18977516. VBIBacSub10457_2947.

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
HOGENOMiHOG000261410.
InParanoidiP37945.
KOiK01338.
OMAiVNIAQRY.
PhylomeDBiP37945.

Enzyme and pathway databases

BioCyciBSUB:BSU28200-MONOMER.
BRENDAi3.4.21.53. 658.

Miscellaneous databases

EvolutionaryTraceiP37945.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLON1_BACSU
AccessioniPrimary (citable) accession number: P37945
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.