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Protein

Lon protease 1

Gene

lon1

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner (By similarity). Has been implicated in preventing sigma(G) activity under non-sporulation conditions.UniRule annotation

Catalytic activityi

Hydrolysis of proteins in presence of ATP.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei677 – 6771UniRule annotation
Active sitei720 – 7201UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi354 – 3618ATP

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU28200-MONOMER.
BRENDAi3.4.21.53. 658.

Protein family/group databases

MEROPSiS16.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Lon protease 1UniRule annotation (EC:3.4.21.53UniRule annotation)
Alternative name(s):
ATP-dependent protease La 1UniRule annotation
Gene namesi
Name:lon1UniRule annotation
Synonyms:lonA
Ordered Locus Names:BSU28200
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 774774Lon protease 1PRO_0000076116Add
BLAST

Proteomic databases

PaxDbiP37945.

Expressioni

Inductioni

By heat shock.UniRule annotation1 Publication

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity (By similarity). Exists as a mixture of small oligomeric species in solution.UniRule annotation1 Publication

Protein-protein interaction databases

IntActiP37945. 2 interactions.
MINTiMINT-8366587.
STRINGi224308.Bsubs1_010100015406.

Structurei

Secondary structure

1
774
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 1612Combined sources
Beta strandi24 – 296Combined sources
Helixi32 – 4110Combined sources
Turni42 – 454Combined sources
Beta strandi46 – 5510Combined sources
Helixi63 – 653Combined sources
Beta strandi68 – 8013Combined sources
Beta strandi86 – 10318Combined sources
Beta strandi105 – 11410Combined sources
Helixi124 – 15532Combined sources
Helixi160 – 17011Combined sources
Helixi175 – 1828Combined sources
Helixi187 – 20721Combined sources
Turni248 – 2503Combined sources
Helixi251 – 2577Combined sources
Beta strandi258 – 2603Combined sources
Helixi264 – 27411Combined sources
Beta strandi280 – 2823Combined sources
Turni284 – 2874Combined sources
Helixi288 – 29811Combined sources
Turni311 – 3133Combined sources
Helixi314 – 3163Combined sources
Helixi318 – 3214Combined sources
Helixi326 – 34116Combined sources
Beta strandi342 – 3443Combined sources
Beta strandi349 – 35911Combined sources
Helixi360 – 37112Combined sources
Beta strandi374 – 3785Combined sources
Helixi404 – 4096Combined sources
Beta strandi413 – 42513Combined sources
Helixi439 – 4413Combined sources
Turni444 – 4485Combined sources
Beta strandi453 – 4553Combined sources
Beta strandi465 – 4695Combined sources
Turni473 – 4753Combined sources
Helixi478 – 4836Combined sources
Beta strandi484 – 4885Combined sources
Helixi494 – 50310Combined sources
Helixi505 – 5128Combined sources
Helixi517 – 5193Combined sources
Helixi524 – 53411Combined sources
Beta strandi538 – 5403Combined sources
Helixi541 – 55919Combined sources
Beta strandi560 – 5623Combined sources
Turni570 – 5734Combined sources
Helixi574 – 5774Combined sources
Turni586 – 5883Combined sources
Beta strandi592 – 60211Combined sources
Beta strandi605 – 61814Combined sources
Beta strandi622 – 6276Combined sources
Helixi630 – 64314Combined sources
Helixi648 – 6503Combined sources
Turni657 – 6593Combined sources
Beta strandi661 – 6666Combined sources
Helixi672 – 6787Combined sources
Helixi679 – 69012Combined sources
Beta strandi710 – 7123Combined sources
Helixi717 – 72610Combined sources
Beta strandi730 – 7356Combined sources
Helixi736 – 7449Combined sources
Helixi747 – 7504Combined sources
Beta strandi757 – 7604Combined sources
Helixi761 – 7688Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X37NMR-A490-604[»]
3M65X-ray2.60A/B1-209[»]
3M6AX-ray3.40A/B/C/D/E/F240-774[»]
ProteinModelPortaliP37945.
SMRiP37945. Positions 4-209, 246-770.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37945.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 200192Lon N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini590 – 771182Lon proteolyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S16 family.UniRule annotation
Contains 1 Lon N-terminal domain.PROSITE-ProRule annotation
Contains 1 Lon proteolytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
HOGENOMiHOG000261410.
InParanoidiP37945.
KOiK01338.
OMAiVNIAQRY.
PhylomeDBiP37945.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37945-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEELKRSIP LLPLRGLLVY PTMVLHLDVG RDKSVQALEQ AMMHDHMIFL
60 70 80 90 100
ATQQDISIDE PGEDEIFTVG TYTKIKQMLK LPNGTIRVLV EGLKRAHIVK
110 120 130 140 150
YNEHEDYTSV DIQLIHEDDS KDTEDEALMR TLLDHFDQYI KISKKISAET
160 170 180 190 200
YAAVTDIEEP GRMADIVASH LPLKLKDKQD ILETADVKDR LNKVIDFINN
210 220 230 240 250
EKEVLEIEKK IGQRVKRSME RTQKEYYLRE QMKAIQKELG DKEGKTGEVQ
260 270 280 290 300
TLTEKIEEAG MPDHVKETAL KELNRYEKIP SSSAESSVIR NYIDWLVALP
310 320 330 340 350
WTDETDDKLD LKEAGRLLDE EHHGLEKVKE RILEYLAVQK LTKSLKGPIL
360 370 380 390 400
CLAGPPGVGK TSLAKSIAKS LGRKFVRISL GGVRDESEIR GHRRTYVGAM
410 420 430 440 450
PGRIIQGMKK AGKLNPVFLL DEIDKMSSDF RGDPSSAMLE VLDPEQNSSF
460 470 480 490 500
SDHYIEETFD LSKVLFIATA NNLATIPGPL RDRMEIINIA GYTEIEKLEI
510 520 530 540 550
VKDHLLPKQI KEHGLKKSNL QLRDQAILDI IRYYTREAGV RSLERQLAAI
560 570 580 590 600
CRKAAKAIVA EERKRITVTE KNLQDFIGKR IFRYGQAETE DQVGVVTGLA
610 620 630 640 650
YTTVGGDTLS IEVSLSPGKG KLILTGKLGD VMRESAQAAF SYVRSKTEEL
660 670 680 690 700
GIEPDFHEKY DIHIHVPEGA VPKDGPSAGI TMATALVSAL TGRAVSREVG
710 720 730 740 750
MTGEITLRGR VLPIGGLKEK ALGAHRAGLT TIIAPKDNEK DIEDIPESVR
760 770
EGLTFILASH LDEVLEHALV GEKK
Length:774
Mass (Da):86,607
Last modified:October 1, 1994 - v1
Checksum:iBE339D896FCEF533
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76424 Genomic DNA. Translation: CAA53984.1.
Z75208 Genomic DNA. Translation: CAA99540.1.
AL009126 Genomic DNA. Translation: CAB14780.1.
PIRiI40421.
RefSeqiNP_390698.1. NC_000964.3.
WP_003229618.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14780; CAB14780; BSU28200.
GeneIDi937486.
KEGGibsu:BSU28200.
PATRICi18977516. VBIBacSub10457_2947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76424 Genomic DNA. Translation: CAA53984.1.
Z75208 Genomic DNA. Translation: CAA99540.1.
AL009126 Genomic DNA. Translation: CAB14780.1.
PIRiI40421.
RefSeqiNP_390698.1. NC_000964.3.
WP_003229618.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X37NMR-A490-604[»]
3M65X-ray2.60A/B1-209[»]
3M6AX-ray3.40A/B/C/D/E/F240-774[»]
ProteinModelPortaliP37945.
SMRiP37945. Positions 4-209, 246-770.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP37945. 2 interactions.
MINTiMINT-8366587.
STRINGi224308.Bsubs1_010100015406.

Protein family/group databases

MEROPSiS16.001.

Proteomic databases

PaxDbiP37945.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14780; CAB14780; BSU28200.
GeneIDi937486.
KEGGibsu:BSU28200.
PATRICi18977516. VBIBacSub10457_2947.

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
HOGENOMiHOG000261410.
InParanoidiP37945.
KOiK01338.
OMAiVNIAQRY.
PhylomeDBiP37945.

Enzyme and pathway databases

BioCyciBSUB:BSU28200-MONOMER.
BRENDAi3.4.21.53. 658.

Miscellaneous databases

EvolutionaryTraceiP37945.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLON1_BACSU
AccessioniPrimary (citable) accession number: P37945
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.