ATP-dependent protease La 1 - Bacillus subtilis

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Reviewed, UniProtKB/Swiss-Prot P37945 (LON1_BACSU)

Last modified June 16, 2009. Version 80. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
ATP-dependent protease La 1
EC=3.4.21.53

Gene names

Name:	lonA
Synonyms:	lon
Ordered Locus Names:	BSU28200

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length	774 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Degrades short-lived regulatory and abnormal proteins in the presence of ATP. Hydrolyzes two ATP for each peptide bond cleaved in the protein substrate By similarity. Has been implicated in preventing sigma(G) activity under non-sporulation conditions.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subcellular location	Cytoplasm.
Induction	By heat shock.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

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Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 774

774

ATP-dependent protease La 1

PRO_0000076116

Regions

Domain

8 – 200

193

Lon

Nucleotide binding

354 – 361

ATP Potential

Sites

Active site

677

By similarity

Active site

720

By similarity

Secondary structure

774

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P37945-1 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: BE339D896FCEF533
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FASTA

774

86,607

        10         20         30         40         50         60 
MAEELKRSIP LLPLRGLLVY PTMVLHLDVG RDKSVQALEQ AMMHDHMIFL ATQQDISIDE 

        70         80         90        100        110        120 
PGEDEIFTVG TYTKIKQMLK LPNGTIRVLV EGLKRAHIVK YNEHEDYTSV DIQLIHEDDS 

       130        140        150        160        170        180 
KDTEDEALMR TLLDHFDQYI KISKKISAET YAAVTDIEEP GRMADIVASH LPLKLKDKQD 

       190        200        210        220        230        240 
ILETADVKDR LNKVIDFINN EKEVLEIEKK IGQRVKRSME RTQKEYYLRE QMKAIQKELG 

       250        260        270        280        290        300 
DKEGKTGEVQ TLTEKIEEAG MPDHVKETAL KELNRYEKIP SSSAESSVIR NYIDWLVALP 

       310        320        330        340        350        360 
WTDETDDKLD LKEAGRLLDE EHHGLEKVKE RILEYLAVQK LTKSLKGPIL CLAGPPGVGK 

       370        380        390        400        410        420 
TSLAKSIAKS LGRKFVRISL GGVRDESEIR GHRRTYVGAM PGRIIQGMKK AGKLNPVFLL 

       430        440        450        460        470        480 
DEIDKMSSDF RGDPSSAMLE VLDPEQNSSF SDHYIEETFD LSKVLFIATA NNLATIPGPL 

       490        500        510        520        530        540 
RDRMEIINIA GYTEIEKLEI VKDHLLPKQI KEHGLKKSNL QLRDQAILDI IRYYTREAGV 

       550        560        570        580        590        600 
RSLERQLAAI CRKAAKAIVA EERKRITVTE KNLQDFIGKR IFRYGQAETE DQVGVVTGLA 

       610        620        630        640        650        660 
YTTVGGDTLS IEVSLSPGKG KLILTGKLGD VMRESAQAAF SYVRSKTEEL GIEPDFHEKY 

       670        680        690        700        710        720 
DIHIHVPEGA VPKDGPSAGI TMATALVSAL TGRAVSREVG MTGEITLRGR VLPIGGLKEK 

       730        740        750        760        770 
ALGAHRAGLT TIIAPKDNEK DIEDIPESVR EGLTFILASH LDEVLEHALV GEKK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, nucleotide sequence, and expression of the Bacillus subtilis lon gene." Riethdorf S., Voelker U., Gerth U., Winkler A., Engelmann S., Hecker M. J. Bacteriol. 176:6518-6527(1994) [PubMed: 7961402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / IS58.
[2]	"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism." Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R. Microbiology 142:3067-3078(1996) [PubMed: 8969504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

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Cross-references

Sequence databases

X76424 Genomic DNA. Translation: CAA53984.1.
Z75208 Genomic DNA. Translation: CAA99540.1.
AL009126 Genomic DNA. Translation: CAB14780.1.

PIR

I40421.

RefSeq

NP_390698.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X37	NMR	-	A	490-604	[»]

ModBase

Search...

Protein family/group databases

MEROPS

S16.001.

Genome annotation databases

GeneID

937486.

GenomeReviews

Gene locus BSU28200 in contig AL009126_GR.

KEGG

bsu:BSU28200.

NMPDR

fig|224308.1.peg.2823.

Organism-specific databases

SubtiList

BG10338. lonA. [Micado]

CMR

Search...

Phylogenomic databases

HOGENOM

P37945.

OMA

P37945. VMKESIQ.

Enzyme and pathway databases

BioCyc

BSUB224308:BSU2816-MON.

BRENDA

3.4.21.53. 150.

Family and domain databases

InterPro

IPR003593. ATPase_AAA+_core.
IPR003959. ATPase_AAA_core.
IPR008269. Pept_S16_C.
IPR004815. Pept_S16_lon.
IPR003111. Pept_S16_N.
IPR008268. Peptidase_S16_AS.
IPR001984. Peptidase_S16_C.
[Graphical view]

Pfam

PF00004. AAA. 1 hit.
PF02190. LON. 1 hit.
PF05362. Lon_C. 1 hit.
[Graphical view]

PRINTS

PR00830. ENDOLAPTASE.

SMART

SM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00763. lon. 1 hit.

PROSITE

PS01046. LON_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

LON1_BACSU

Accession

Primary (citable) accession number: P37945

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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