Reviewed, UniProtKB/Swiss-Prot P37943 (LEPP_BACNA)

Names and origin

Protein names

Recommended name:
    Signal peptidase I P
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:

sipP

Encoded on

Plasmid pTA1015

Organism

Bacillus subtilis subsp. natto

Taxonomic identifier

86029 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protease
Technical term	Plasmid
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	Signal peptidase I P		PRO_0000109497
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 35	20	Potential
	Topological domain	36 – 186	151	Extracellular Potential
Sites
	Active site	44	1	By similarity
	Active site	86	1	By similarity
Experimental info
	Mutagenesis	62	1	K → N: No effect.

Sequences

	Sequence		Length	Mass (Da)
	P37943-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 3DB7ECBE5F2334BB	FASTA	186	21,252
	10 20 30 40 50 60 MTKEKVFKKK SSILEWGKAI VIAVILALLI RNFLFEPYVV EGKSMDPTLV DSERLFVNKT 70 80 90 100 110 120 VKYTGNFKRG DIIILNGKEK STHYVKRLIG LPGDTVEMKN DHLFINGNEV KEPYLSYNKE 130 140 150 160 170 180 NAKKVGINLT GDFGPIKVPK DKYFVMGDNR QESMDSRNGL GLFTKDDIQG TEEFVFFPFS NMRKAK

References

[1]

"The endogenous Bacillus subtilis (natto) plasmids pTA1015 and pTA1040 contain signal peptidase-encoding genes: identification of a new structural module on cryptic plasmids." Meijer W.J.J., de Jong A., Bea G., Wisman A., Tjalsma H., Venema G., Bron S., van Dijl J.M. Mol. Microbiol. 17:621-631(1995) [PubMed: 8801417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS. Strain: IAM 1028.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z27459 Genomic DNA. Translation: CAA81815.1. U32379 Genomic DNA. Translation: AAC44415.1.
PIR	I40470.
RefSeq	NP_053783.1.
3D structure databases
HSSP	HSSP built from PDB template 1B12 based on UniProtKB P00803.
ModBase	Search...
Protein family/group databases
MEROPS	S26.007.
Genome annotation databases
GeneID	1115974.
Enzyme and pathway databases
BRENDA	3.4.21.89. 298357.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEPP_BACNA

Accession

Primary (citable) accession number: P37943

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)