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P37941

- ODBB_BACSU

UniProt

P37941 - ODBB_BACSU

Protein

2-oxoisovalerate dehydrogenase subunit beta

Gene

bfmBAB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

    Catalytic activityi

    3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2.

    Cofactori

    Thiamine pyrophosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei29 – 291Thiamine pyrophosphateBy similarity
    Binding sitei82 – 821Thiamine pyrophosphateBy similarity
    Active sitei129 – 1291Proton acceptorBy similarity
    Binding sitei129 – 1291SubstrateBy similarity

    GO - Molecular functioni

    1. 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciBSUB:BSU24040-MONOMER.
    MetaCyc:MONOMER-11684.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-oxoisovalerate dehydrogenase subunit beta (EC:1.2.4.4)
    Alternative name(s):
    Branched-chain alpha-keto acid dehydrogenase E1 component beta chain
    Short name:
    BCKDH E1-beta
    Gene namesi
    Name:bfmBAB
    Synonyms:bfmB1b
    Ordered Locus Names:BSU24040
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU24040. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3273272-oxoisovalerate dehydrogenase subunit betaPRO_0000162250Add
    BLAST

    Proteomic databases

    PaxDbiP37941.

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha and two beta chains. Directly associated with ODBA in the E1 complex.

    Protein-protein interaction databases

    IntActiP37941. 2 interactions.
    STRINGi224308.BSU24040.

    Structurei

    3D structure databases

    ProteinModelPortaliP37941.
    SMRiP37941. Positions 2-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni58 – 603Thiamine pyrophosphate bindingBy similarity
    Regioni83 – 864Substrate bindingBy similarity
    Regioni86 – 894Thiamine pyrophosphate bindingBy similarity

    Phylogenomic databases

    eggNOGiCOG0022.
    HOGENOMiHOG000281451.
    KOiK00167.
    OMAiDKHRCLA.
    OrthoDBiEOG6JQH4C.
    PhylomeDBiP37941.

    Family and domain databases

    Gene3Di3.40.50.920. 1 hit.
    3.40.50.970. 1 hit.
    InterProiIPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005475. Transketolase-like_Pyr-bd.
    IPR005476. Transketolase_C.
    [Graphical view]
    PfamiPF02779. Transket_pyr. 1 hit.
    PF02780. Transketolase_C. 1 hit.
    [Graphical view]
    SMARTiSM00861. Transket_pyr. 1 hit.
    [Graphical view]
    SUPFAMiSSF52518. SSF52518. 1 hit.
    SSF52922. SSF52922. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P37941-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVMSYIDAI NLAMKEEMER DSRVFVLGED VGRKGGVFKA TAGLYEQFGE    50
    ERVMDTPLAE SAIAGVGIGA AMYGMRPIAE MQFADFIMPA VNQIISEAAK 100
    IRYRSNNDWS CPIVVRAPYG GGVHGALYHS QSVEAIFANQ PGLKIVMPST 150
    PYDAKGLLKA AVRDEDPVLF FEHKRAYRLI KGEVPADDYV LPIGKADVKR 200
    EGDDITVITY GLCVHFALQA AERLEKDGIS AHVVDLRTVY PLDKEAIIEA 250
    ASKTGKVLLV TEDTKEGSIM SEVAAIISEH CLFDLDAPIK RLAGPDIPAM 300
    PYAPTMEKYF MVNPDKVEAA MRELAEF 327
    Length:327
    Mass (Da):35,856
    Last modified:October 1, 1994 - v1
    Checksum:iF19F5BF8F413EBFE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97391 Genomic DNA. Translation: AAA22279.1.
    D84432 Genomic DNA. Translation: BAA12599.1.
    AL009126 Genomic DNA. Translation: CAB14335.1.
    PIRiD69593.
    RefSeqiNP_390284.1. NC_000964.3.
    WP_004398638.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB14335; CAB14335; BSU24040.
    GeneIDi938672.
    KEGGibsu:BSU24040.
    PATRICi18976636. VBIBacSub10457_2508.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97391 Genomic DNA. Translation: AAA22279.1 .
    D84432 Genomic DNA. Translation: BAA12599.1 .
    AL009126 Genomic DNA. Translation: CAB14335.1 .
    PIRi D69593.
    RefSeqi NP_390284.1. NC_000964.3.
    WP_004398638.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali P37941.
    SMRi P37941. Positions 2-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P37941. 2 interactions.
    STRINGi 224308.BSU24040.

    Proteomic databases

    PaxDbi P37941.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14335 ; CAB14335 ; BSU24040 .
    GeneIDi 938672.
    KEGGi bsu:BSU24040.
    PATRICi 18976636. VBIBacSub10457_2508.

    Organism-specific databases

    GenoListi BSU24040. [Micado ]

    Phylogenomic databases

    eggNOGi COG0022.
    HOGENOMi HOG000281451.
    KOi K00167.
    OMAi DKHRCLA.
    OrthoDBi EOG6JQH4C.
    PhylomeDBi P37941.

    Enzyme and pathway databases

    BioCyci BSUB:BSU24040-MONOMER.
    MetaCyc:MONOMER-11684.

    Family and domain databases

    Gene3Di 3.40.50.920. 1 hit.
    3.40.50.970. 1 hit.
    InterProi IPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005475. Transketolase-like_Pyr-bd.
    IPR005476. Transketolase_C.
    [Graphical view ]
    Pfami PF02779. Transket_pyr. 1 hit.
    PF02780. Transketolase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00861. Transket_pyr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52518. SSF52518. 1 hit.
    SSF52922. SSF52922. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The primary structure of branched-chain alpha-oxo acid dehydrogenase from Bacillus subtilis and its similarity to other alpha-oxo acid dehydrogenases."
      Wang G.-F., Kuriki T., Roy K.L., Kaneda T.
      Eur. J. Biochem. 213:1091-1099(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-32.
      Strain: 168.
    2. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
      Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
      Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.

    Entry informationi

    Entry nameiODBB_BACSU
    AccessioniPrimary (citable) accession number: P37941
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

    External Data

    Dasty 3