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Protein

Ribonuclease PH

Gene

rph

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.UniRule annotation

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei88Phosphate (substrate) bindingUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processrRNA processing, tRNA processing

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-1277-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PHUniRule annotation (EC:2.7.7.56UniRule annotation)
Short name:
RNase PHUniRule annotation
Alternative name(s):
tRNA nucleotidyltransferaseUniRule annotation
Gene namesi
Name:rphUniRule annotation
Synonyms:rnpH, rphA
Ordered Locus Names:ML1174
ORF Names:B1549_C1_182
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1174.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001399101 – 259Ribonuclease PHAdd BLAST259

Interactioni

Subunit structurei

Homohexameric ring arranged as a trimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML1174.

Structurei

3D structure databases

ProteinModelPortaliP37939.
SMRiP37939.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 128Phosphate (substrate) bindingUniRule annotation3

Sequence similaritiesi

Belongs to the RNase PH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105ED0. Bacteria.
COG0689. LUCA.
HOGENOMiHOG000229516.
KOiK00989.
OMAiKGKGQGW.
OrthoDBiPOG091H03ML.

Family and domain databases

CDDicd11362. RNase_PH_bact. 1 hit.
Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR036345. ExoRNase_PH_dom2_sf.
IPR027408. PNPase/RNase_PH_dom_sf.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
PfamiView protein in Pfam
PF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiView protein in PROSITE
PS01277. RIBONUCLEASE_PH. 1 hit.

Sequencei

Sequence statusi: Complete.

P37939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTRADGRLD DELRAVVITR GFTEHPAGSV LIEFGHTKVM CTASPTEGVP
60 70 80 90 100
RWRKGSGKGW LTAEYAMLPS ATHTRSERES VKGRLSGRTQ EISRLIGRSL
110 120 130 140 150
RACIDLAALG ENTIAVDCDV LQADGGTRTA AVTGAYVALA DAVTYLSAAG
160 170 180 190 200
RLLDPRPLSC AIAAVSVGVV DGRIRVDLSY EEDSRAEVDM NVIATDTGTL
210 220 230 240 250
VEIQGTGEGA TFPRSTLDKL LDMALGACDK LFVAQRDALA LPYQGMSPEG

PPPPKAFGS
Length:259
Mass (Da):27,365
Last modified:October 1, 1994 - v1
Checksum:iA258C5C054D17A59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00014 Genomic DNA. Translation: AAA50880.1.
AL583921 Genomic DNA. Translation: CAC31555.1.
PIRiS72788.
RefSeqiNP_301856.1. NC_002677.1.
WP_010908180.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31555; CAC31555; CAC31555.
GeneIDi910272.
KEGGimle:ML1174.
PATRICifig|272631.5.peg.2131.

Similar proteinsi

Entry informationi

Entry nameiRNPH_MYCLE
AccessioniPrimary (citable) accession number: P37939
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: February 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome