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Protein

Mitochondrial intermediate peptidase

Gene

OCT1

Organism
Schizophyllum commune (Split gill fungus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane (By similarity).By similarity

Catalytic activityi

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi558 – 5581Zinc; catalyticPROSITE-ProRule annotation
Active sitei559 – 5591PROSITE-ProRule annotation
Metal bindingi562 – 5621Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi565 – 5651Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM03.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial intermediate peptidase (EC:3.4.24.59)
Short name:
MIP
Alternative name(s):
Octapeptidyl aminopeptidase
Gene namesi
Name:OCT1
Synonyms:MEP, MIP
OrganismiSchizophyllum commune (Split gill fungus)
Taxonomic identifieri5334 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesSchizophyllaceaeSchizophyllum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2828MitochondrionSequence AnalysisAdd
BLAST
Chaini29 – 775747Mitochondrial intermediate peptidasePRO_0000028581Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi578458.XP_003038723.1.

Structurei

3D structure databases

ProteinModelPortaliP37932.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.1370.10. 2 hits.
3.40.390.10. 2 hits.
InterProiIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIARPARDVL SSATKKQFRF RGCLAARHEP YHTSTSRAGQ VAILPATTDD
60 70 80 90 100
KTLVSVFDSP RSNAKLSAFA TTGLFNHSTV THPRALNSIA QGTLIRAHVL
110 120 130 140 150
TNRILRAKES REELFKVVKN LDRLSDMLCS VIDLCELVRN SHPDRAWVEA
160 170 180 190 200
ANDAYEGLCQ TMNELNTHVG LYDVLKIVLS DPEIVKSLSP EAYRTAMIFW
210 220 230 240 250
NDFEKSAINL PAKEREEFVA LSSEIISLGR MFLEETTAAR PPAKIPPSDL
260 270 280 290 300
AGLKDKGMGV RLQLQAQFTQ RDLHVYPGSL QAQMIMRSAP AEEARRRVYI
310 320 330 340 350
ASHSSTPEQI ELLERMLSTR ARLARLVGRE SFAAMALDDK MAKNPTNVAR
360 370 380 390 400
FLDSLMDRSR PYARRALRNL SMRKQEHLHT PPFPTIQAWD RDYYCPPEPP
410 420 430 440 450
APPIPLPRLT FGTVLMGLSR LFRHLYGIHL RPVKPIAGEV WHSDVHKLEV
460 470 480 490 500
VDEERGVIGL IYADVFARRG KASGAAHYTV RCSRRTDDDD VQGDNDELTR
510 520 530 540 550
MYPDLIKQSE EFEAVGRGPI PGLPGTYQQP LVVLLCEFAR PSLGAAVLEW
560 570 580 590 600
HEVMTLFHEM GHAMHSMIGR TEYQNVSGTR CPTDFVELPS ILMEHFLNSR
610 620 630 640 650
QVLSLFHADS TSSSSQPIGN HDEDPCHSID TYAQIMLAAL DQIYHSPAAL
660 670 680 690 700
QPGFDSTRKL ARLHDEKGLI PYVPGTSFQT QFGHLFGYGA TYYSYLFDRA
710 720 730 740 750
IASRVWKDVF SSSPLSRETG ERYKQEVLRY GGGKDPWEMV SALLKAPELA
760 770
SGDAEAMATV GRWKIEDEVG LPGRH
Length:775
Mass (Da):86,915
Last modified:October 1, 1996 - v2
Checksum:i875772B6D9560C55
GO

Sequence cautioni

The sequence AAB01366.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti486 – 4861T → D (PubMed:1353886).Curated
Sequence conflicti621 – 6222HD → QH in AAB01366 (PubMed:1353886).Curated
Sequence conflicti621 – 6222HD → QH in AAB01368 (PubMed:1353886).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti572 – 5721E → G in strain: UVM 9-4.
Natural varianti651 – 6511Q → E in strain: UVM 9-1 and CBS 340.81 / UVM 4-40.
Natural varianti659 – 6591K → E in strain: UVM 9-1 and CBS 340.81 / UVM 4-40.
Natural varianti729 – 7291R → Q in strain: UVM 9-1.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43072 Genomic DNA. Translation: AAA93531.1.
M97179 Genomic DNA. Translation: AAB01366.1. Different initiation.
M97180 Genomic DNA. Translation: AAB01368.1.
M97181 Genomic DNA. Translation: AAB01371.1.
PIRiE37271.
F37271.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43072 Genomic DNA. Translation: AAA93531.1.
M97179 Genomic DNA. Translation: AAB01366.1. Different initiation.
M97180 Genomic DNA. Translation: AAB01368.1.
M97181 Genomic DNA. Translation: AAB01371.1.
PIRiE37271.
F37271.

3D structure databases

ProteinModelPortaliP37932.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi578458.XP_003038723.1.

Protein family/group databases

MEROPSiM03.006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.1370.10. 2 hits.
3.40.390.10. 2 hits.
InterProiIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mammalian mitochondrial intermediate peptidase: structure/function analysis of a new homologue from Schizophyllum commune and relationship to thimet oligopeptidases."
    Isaya G., Sakati W.R., Rollins R.A., Shen G.P., Hanson L.C., Ullrich R.C., Novotny C.P.
    Genomics 28:450-461(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
    Strain: 1-71.
  2. "The A alpha mating locus of Schizophyllum commune encodes two dissimilar multiallelic homeodomain proteins."
    Stankis M.M., Specht C.A., Yang H., Giasson L., Ullrich R.C., Novotny C.P.
    Proc. Natl. Acad. Sci. U.S.A. 89:7169-7173(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 486-775.
    Strain: ATCC 44201 / CBS 340.81 / UVM 4-40 / 4-40, UVM 9-1 and UVM 9-4.

Entry informationi

Entry nameiPMIP_SCHCO
AccessioniPrimary (citable) accession number: P37932
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.