Reviewed, UniProtKB/Swiss-Prot P37932 (PMIP_SCHCO)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
Synonyms:	MEP, MIP

Organism

Schizophyllum commune (Bracket fungus)

Taxonomic identifier

5334 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Schizophyllaceae › Schizophyllum

Protein attributes

Sequence length	775 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix. Ref.1
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 28	28	Mitochondrion Potential
	Chain	29 – 775	747	Mitochondrial intermediate peptidase		PRO_0000028581
Sites
	Active site	559	1	By similarity
	Metal binding	558	1	Zinc; catalytic By similarity
	Metal binding	562	1	Zinc; catalytic By similarity
	Metal binding	565	1	Zinc; catalytic By similarity
Natural variations
	Natural variant	572	1	E → G in strain: UVM 9-4.
	Natural variant	651	1	Q → E in strain: UVM 9-1 and CBS 340.81 / UVM 4-40.
	Natural variant	659	1	K → E in strain: UVM 9-1 and CBS 340.81 / UVM 4-40.
	Natural variant	729	1	R → Q in strain: UVM 9-1.
Experimental info
	Sequence conflict	486	1	T → D Ref.2
	Sequence conflict	621 – 622	2	HD → QH in AAB01366. Ref.2
	Sequence conflict	621 – 622	2	HD → QH in AAB01368. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P37932-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 875772B6D9560C55	FASTA	775	86,915
	10 20 30 40 50 60 MIARPARDVL SSATKKQFRF RGCLAARHEP YHTSTSRAGQ VAILPATTDD KTLVSVFDSP 70 80 90 100 110 120 RSNAKLSAFA TTGLFNHSTV THPRALNSIA QGTLIRAHVL TNRILRAKES REELFKVVKN 130 140 150 160 170 180 LDRLSDMLCS VIDLCELVRN SHPDRAWVEA ANDAYEGLCQ TMNELNTHVG LYDVLKIVLS 190 200 210 220 230 240 DPEIVKSLSP EAYRTAMIFW NDFEKSAINL PAKEREEFVA LSSEIISLGR MFLEETTAAR 250 260 270 280 290 300 PPAKIPPSDL AGLKDKGMGV RLQLQAQFTQ RDLHVYPGSL QAQMIMRSAP AEEARRRVYI 310 320 330 340 350 360 ASHSSTPEQI ELLERMLSTR ARLARLVGRE SFAAMALDDK MAKNPTNVAR FLDSLMDRSR 370 380 390 400 410 420 PYARRALRNL SMRKQEHLHT PPFPTIQAWD RDYYCPPEPP APPIPLPRLT FGTVLMGLSR 430 440 450 460 470 480 LFRHLYGIHL RPVKPIAGEV WHSDVHKLEV VDEERGVIGL IYADVFARRG KASGAAHYTV 490 500 510 520 530 540 RCSRRTDDDD VQGDNDELTR MYPDLIKQSE EFEAVGRGPI PGLPGTYQQP LVVLLCEFAR 550 560 570 580 590 600 PSLGAAVLEW HEVMTLFHEM GHAMHSMIGR TEYQNVSGTR CPTDFVELPS ILMEHFLNSR 610 620 630 640 650 660 QVLSLFHADS TSSSSQPIGN HDEDPCHSID TYAQIMLAAL DQIYHSPAAL QPGFDSTRKL 670 680 690 700 710 720 ARLHDEKGLI PYVPGTSFQT QFGHLFGYGA TYYSYLFDRA IASRVWKDVF SSSPLSRETG 730 740 750 760 770 ERYKQEVLRY GGGKDPWEMV SALLKAPELA SGDAEAMATV GRWKIEDEVG LPGRH

References

[1]	"Mammalian mitochondrial intermediate peptidase: structure/function analysis of a new homologue from Schizophyllum commune and relationship to thimet oligopeptidases." Isaya G., Sakati W.R., Rollins R.A., Shen G.P., Hanson L.C., Ullrich R.C., Novotny C.P. Genomics 28:450-461(1995) [PubMed: 7490080] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION. Strain: 1-71.
[2]	"The A alpha mating locus of Schizophyllum commune encodes two dissimilar multiallelic homeodomain proteins." Stankis M.M., Specht C.A., Yang H., Giasson L., Ullrich R.C., Novotny C.P. Proc. Natl. Acad. Sci. U.S.A. 89:7169-7173(1992) [PubMed: 1353886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 486-775. Strain: ATCC 44201 / CBS 340.81 / UVM 4-40 / 4-40, UVM 9-1 and UVM 9-4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43072 Genomic DNA. Translation: AAA93531.1. M97179 Genomic DNA. Translation: AAB01366.1. Different initiation. M97180 Genomic DNA. Translation: AAB01368.1. M97181 Genomic DNA. Translation: AAB01371.1.
PIR	E37271. F37271.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Enzyme and pathway databases
BRENDA	3.4.24.59. 15239.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_SCHCO

Accession

Primary (citable) accession number: P37932

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)