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P37913

- DNLI1_MOUSE

UniProt

P37913 - DNLI1_MOUSE

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Protein

DNA ligase 1

Gene
Lig1, Lig-1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactori

Magnesium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei303 – 3031Interaction with target DNA By similarity
Binding sitei564 – 5641ATP By similarity
Active sitei566 – 5661N6-AMP-lysine intermediate By similarity
Binding sitei571 – 5711ATP By similarity
Binding sitei587 – 5871ATP By similarity
Sitei588 – 5881Interaction with target DNA By similarity
Metal bindingi619 – 6191Magnesium 1 By similarity
Metal bindingi718 – 7181Magnesium 2 By similarity
Binding sitei723 – 7231ATP By similarity
Binding sitei736 – 7361ATP By similarity
Binding sitei742 – 7421ATP By similarity
Sitei768 – 7681Interaction with target DNA By similarity
Sitei793 – 7931Interaction with target DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: RefGenome
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA ligation Source: RefGenome
  4. DNA recombination Source: UniProtKB-KW
  5. DNA repair Source: MGI
  6. DNA replication Source: MGI
  7. double-strand break repair via nonhomologous end joining Source: MGI
  8. lagging strand elongation Source: RefGenome
  9. response to hydrogen peroxide Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 1 (EC:6.5.1.1)
Alternative name(s):
DNA ligase I
Polydeoxyribonucleotide synthase [ATP] 1
Gene namesi
Name:Lig1
Synonyms:Lig-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:101789. Lig1.

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: RefGenome
  2. mitochondrion Source: RefGenome
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 916916DNA ligase 1PRO_0000059571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491Phosphoserine By similarity
Modified residuei51 – 511Phosphoserine3 Publications
Modified residuei65 – 651Phosphoserine1 Publication
Modified residuei77 – 771Phosphothreonine1 Publication
Modified residuei144 – 1441N6-acetyllysine1 Publication
Modified residuei193 – 1931Phosphothreonine By similarity
Modified residuei225 – 2251N6-acetyllysine1 Publication
Modified residuei232 – 2321Phosphothreonine By similarity
Cross-linki420 – 420Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Modified residuei911 – 9111Phosphoserine By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP37913.
PaxDbiP37913.
PRIDEiP37913.

PTM databases

PhosphoSiteiP37913.

Expressioni

Gene expression databases

CleanExiMM_LIG1.
GenevestigatoriP37913.

Interactioni

Protein-protein interaction databases

BioGridi201164. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP37913.
SMRiP37913. Positions 260-899.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni447 – 45610Interaction with target DNA By similarity
Regioni640 – 6423Interaction with target DNA By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1793.
HOVERGENiHBG005514.
InParanoidiP37913.
KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37913-1 [UniParc]FASTAAdd to Basket

« Hide

MQRSIMSFFQ PTKEGKAKKP EKETPSSIRE KEPPPKVALK ERNQVVPESD    50
SPVKRTGRKV AQVLSCEGED EDEAPGTPKV QKPVSDSEQS SPPSPDTCPE 100
NSPVFNCSSP MDISPSGFPK RRTARKQLPK RTIQDTLEEQ NEDKTKTAKK 150
RKKEEETPKE SLAEAEDIKQ KEEKEGDQLI VPSEPTKSPE SVTLTKTENI 200
PVCKAGVKLK PQEEEQSKPP ARGAKTLSSF FTPRKPAVKT EVKQEESGTL 250
RKEETKGTLD PANYNPSKNN YHPIEDACWK HGQKVPFLAV ARTFEKIEEV 300
SARLKMVETL SNLLRSVVAL SPPDLLPVLY LSLNRLGPPQ QGLELGVGDG 350
VLLKAVAQAT GRQLESIRAE VAEKGDVGLV AENSRSTQRL MLPPPPLTIS 400
GVFTKFCDIA RLTGSASMAK KMDIIKGLFV ACRHSEARYI ARSLSGRLRL 450
GLAEQSVLAA LAQAVSLTPP GQEFPTVVVD AGKGKTAEAR KMWLEEQGMI 500
LKQTFCEVPD LDRIIPVLLE HGLERLPEHC KLSPGVPLKP MLAHPTRGVS 550
EVLKRFEEVD FTCEYKYDGQ RAQIHVLEGG EVKIFSRNQE DNTGKYPDII 600
SRIPKIKHPS VTSFILDTEA VAWDREKKQI QPFQVLTTRK RKEVDASEIQ 650
VQVCLYAFDL IYLNGESLVR QPLSRRRQLL RENFVETEGE FVFTTSLDTK 700
DTEQIAEFLE QSVKDSCEGL MVKTLDVDAT YEIAKRSHNW LKLKKDYLDG 750
VGDTLDLVVI GAYLGRGKRA GRYGGFLLAA YDEESEELQA ICKLGTGFSD 800
EELEEHHQSL QALVLPTPRP YVRIDGAVAP DHWLDPSIVW EVKCADLSLS 850
PIYPAARGLV DKEKGISLRF PRFIRVRKDK QPEQATTSNQ VASLYRKQSQ 900
IQNQQSSDLD SDVEDY 916
Length:916
Mass (Da):102,290
Last modified:February 1, 1996 - v2
Checksum:i6310B044364FB0E7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04674 mRNA. Translation: AAA70403.1.
U19604 mRNA. Translation: AAB60500.1.
PIRiI48921.
RefSeqiNP_034845.2. NM_010715.2.
UniGeneiMm.288179.
Mm.421129.

Genome annotation databases

GeneIDi16881.
KEGGimmu:16881.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04674 mRNA. Translation: AAA70403.1 .
U19604 mRNA. Translation: AAB60500.1 .
PIRi I48921.
RefSeqi NP_034845.2. NM_010715.2.
UniGenei Mm.288179.
Mm.421129.

3D structure databases

ProteinModelPortali P37913.
SMRi P37913. Positions 260-899.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201164. 1 interaction.

PTM databases

PhosphoSitei P37913.

Proteomic databases

MaxQBi P37913.
PaxDbi P37913.
PRIDEi P37913.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 16881.
KEGGi mmu:16881.

Organism-specific databases

CTDi 3978.
MGIi MGI:101789. Lig1.

Phylogenomic databases

eggNOGi COG1793.
HOVERGENi HBG005514.
InParanoidi P37913.
KOi K10747.

Miscellaneous databases

NextBioi 290882.
PROi P37913.
SOURCEi Search...

Gene expression databases

CleanExi MM_LIG1.
Genevestigatori P37913.

Family and domain databases

Gene3Di 1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProi IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
Pfami PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SUPFAMi SSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of a cDNA coding for the murine DNA ligase I enzyme."
    Savini E., Biamonti G., Ciarrocchi G., Montecucco A.
    Gene 144:253-257(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fibroblast.
  2. Montecucco A.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Comparison between cDNA clones encoding murine DNA ligase I."
    Jessop J.K., Melton D.W.
    Gene 160:307-308(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/Ola.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51; SER-65 AND THR-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-144 AND LYS-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiDNLI1_MOUSE
AccessioniPrimary (citable) accession number: P37913
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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