Reviewed, UniProtKB/Swiss-Prot P37913 (DNLI1_MOUSE)

Names and origin

Protein names

Recommended name:
    DNA ligase 1
    EC=6.5.1.1
Alternative name(s):
    DNA ligase I
    Polydeoxyribonucleotide synthase [ATP] 1

Gene names

Name:	Lig1
Synonyms:	Lig-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	916 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Subcellular location	Nucleus.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from mutant phenotype. Source: MGI cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 916	916	DNA ligase 1		PRO_0000059571
Sites
	Active site	566	1	N6-AMP-lysine intermediate By similarity
Amino acid modifications
	Modified residue	49	1	Phosphoserine By similarity
	Modified residue	51	1	Phosphoserine By similarity
	Modified residue	183	1	Phosphoserine
	Modified residue	188	1	Phosphoserine
	Modified residue	193	1	Phosphothreonine By similarity
	Modified residue	229	1	Phosphoserine By similarity
	Modified residue	232	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37913-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 6310B044364FB0E7	FASTA	916	102,290
	10 20 30 40 50 60 MQRSIMSFFQ PTKEGKAKKP EKETPSSIRE KEPPPKVALK ERNQVVPESD SPVKRTGRKV 70 80 90 100 110 120 AQVLSCEGED EDEAPGTPKV QKPVSDSEQS SPPSPDTCPE NSPVFNCSSP MDISPSGFPK 130 140 150 160 170 180 RRTARKQLPK RTIQDTLEEQ NEDKTKTAKK RKKEEETPKE SLAEAEDIKQ KEEKEGDQLI 190 200 210 220 230 240 VPSEPTKSPE SVTLTKTENI PVCKAGVKLK PQEEEQSKPP ARGAKTLSSF FTPRKPAVKT 250 260 270 280 290 300 EVKQEESGTL RKEETKGTLD PANYNPSKNN YHPIEDACWK HGQKVPFLAV ARTFEKIEEV 310 320 330 340 350 360 SARLKMVETL SNLLRSVVAL SPPDLLPVLY LSLNRLGPPQ QGLELGVGDG VLLKAVAQAT 370 380 390 400 410 420 GRQLESIRAE VAEKGDVGLV AENSRSTQRL MLPPPPLTIS GVFTKFCDIA RLTGSASMAK 430 440 450 460 470 480 KMDIIKGLFV ACRHSEARYI ARSLSGRLRL GLAEQSVLAA LAQAVSLTPP GQEFPTVVVD 490 500 510 520 530 540 AGKGKTAEAR KMWLEEQGMI LKQTFCEVPD LDRIIPVLLE HGLERLPEHC KLSPGVPLKP 550 560 570 580 590 600 MLAHPTRGVS EVLKRFEEVD FTCEYKYDGQ RAQIHVLEGG EVKIFSRNQE DNTGKYPDII 610 620 630 640 650 660 SRIPKIKHPS VTSFILDTEA VAWDREKKQI QPFQVLTTRK RKEVDASEIQ VQVCLYAFDL 670 680 690 700 710 720 IYLNGESLVR QPLSRRRQLL RENFVETEGE FVFTTSLDTK DTEQIAEFLE QSVKDSCEGL 730 740 750 760 770 780 MVKTLDVDAT YEIAKRSHNW LKLKKDYLDG VGDTLDLVVI GAYLGRGKRA GRYGGFLLAA 790 800 810 820 830 840 YDEESEELQA ICKLGTGFSD EELEEHHQSL QALVLPTPRP YVRIDGAVAP DHWLDPSIVW 850 860 870 880 890 900 EVKCADLSLS PIYPAARGLV DKEKGISLRF PRFIRVRKDK QPEQATTSNQ VASLYRKQSQ 910 IQNQQSSDLD SDVEDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of a cDNA coding for the murine DNA ligase I enzyme." Savini E., Biamonti G., Ciarrocchi G., Montecucco A. Gene 144:253-257(1994) [PubMed: 8039710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fibroblast.
[2]	Montecucco A. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Comparison between cDNA clones encoding murine DNA ligase I." Jessop J.K., Melton D.W. Gene 160:307-308(1995) [PubMed: 7642116] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/Ola.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183 AND SER-188, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U04674 mRNA. Translation: AAA70403.1. U19604 mRNA. Translation: AAB60500.1.
PIR	I48921.
RefSeq	NP_034845.2.
UniGene	Mm.288179 Mm.421129
3D structure databases
SMR	P37913. Positions 260-899.
ModBase	Search...
PTM databases
PhosphoSite	P37913.
Genome annotation databases
Ensembl	ENSMUSG00000056394. Mus musculus. [Contig view]
GeneID	16881.
Organism-specific databases
MGI	MGI:101789. Lig1.
Phylogenomic databases
HOVERGEN	P37913.
Gene expression databases
ArrayExpress	P37913.
CleanEx	MM_LIG1.
GermOnline	ENSMUSG00000056394. Mus musculus.
Family and domain databases
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	290882.
SOURCE	Search...

Entry information

Entry name

DNLI1_MOUSE

Accession

Primary (citable) accession number: P37913

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	February 1, 1996
Last modified:	November 25, 2008
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)