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Protein

DNA ligase 1

Gene

Lig1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.PROSITE-ProRule annotation

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei564ATPBy similarity1
Active sitei566N6-AMP-lysine intermediatePROSITE-ProRule annotation1
Binding sitei571ATPBy similarity1
Binding sitei587ATPBy similarity1
Metal bindingi619Magnesium 1By similarity1
Metal bindingi718Magnesium 2By similarity1
Binding sitei723ATPBy similarity1
Binding sitei736ATPBy similarity1
Binding sitei742ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: MGI
  • cell division Source: UniProtKB-KW
  • DNA biosynthetic process Source: InterPro
  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: MGI
  • DNA replication Source: MGI
  • double-strand break repair via nonhomologous end joining Source: MGI
  • Okazaki fragment processing involved in mitotic DNA replication Source: GO_Central
  • response to hydrogen peroxide Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 1 (EC:6.5.1.1PROSITE-ProRule annotation)
Alternative name(s):
DNA ligase I
Polydeoxyribonucleotide synthase [ATP] 1
Gene namesi
Name:Lig1
Synonyms:Lig-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:101789. Lig1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000595711 – 916DNA ligase 1Add BLAST916

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphoserineBy similarity1
Modified residuei51PhosphoserineCombined sources1
Modified residuei65PhosphoserineCombined sources1
Modified residuei77PhosphothreonineCombined sources1
Modified residuei144N6-acetyllysineCombined sources1
Modified residuei193PhosphothreonineBy similarity1
Modified residuei225N6-acetyllysineCombined sources1
Modified residuei228PhosphoserineBy similarity1
Modified residuei229PhosphoserineBy similarity1
Modified residuei232PhosphothreonineBy similarity1
Modified residuei796PhosphothreonineBy similarity1
Modified residuei799PhosphoserineBy similarity1
Modified residuei906PhosphoserineCombined sources1
Modified residuei907PhosphoserineCombined sources1
Modified residuei911PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP37913.
MaxQBiP37913.
PaxDbiP37913.
PeptideAtlasiP37913.
PRIDEiP37913.

PTM databases

iPTMnetiP37913.
PhosphoSitePlusiP37913.
SwissPalmiP37913.

Expressioni

Gene expression databases

CleanExiMM_LIG1.

Interactioni

Subunit structurei

Interacts with PCNA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei303Interaction with target DNABy similarity1
Sitei588Interaction with target DNABy similarity1
Sitei768Interaction with target DNABy similarity1
Sitei793Interaction with target DNABy similarity1

Protein-protein interaction databases

BioGridi201164. 2 interactors.
STRINGi10090.ENSMUSP00000096411.

Structurei

3D structure databases

ProteinModelPortaliP37913.
SMRiP37913.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni447 – 456Interaction with target DNABy similarity10
Regioni640 – 642Interaction with target DNABy similarity3

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated

Phylogenomic databases

eggNOGiKOG0967. Eukaryota.
COG1793. LUCA.
HOVERGENiHBG005514.
InParanoidiP37913.
KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37913-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRSIMSFFQ PTKEGKAKKP EKETPSSIRE KEPPPKVALK ERNQVVPESD
60 70 80 90 100
SPVKRTGRKV AQVLSCEGED EDEAPGTPKV QKPVSDSEQS SPPSPDTCPE
110 120 130 140 150
NSPVFNCSSP MDISPSGFPK RRTARKQLPK RTIQDTLEEQ NEDKTKTAKK
160 170 180 190 200
RKKEEETPKE SLAEAEDIKQ KEEKEGDQLI VPSEPTKSPE SVTLTKTENI
210 220 230 240 250
PVCKAGVKLK PQEEEQSKPP ARGAKTLSSF FTPRKPAVKT EVKQEESGTL
260 270 280 290 300
RKEETKGTLD PANYNPSKNN YHPIEDACWK HGQKVPFLAV ARTFEKIEEV
310 320 330 340 350
SARLKMVETL SNLLRSVVAL SPPDLLPVLY LSLNRLGPPQ QGLELGVGDG
360 370 380 390 400
VLLKAVAQAT GRQLESIRAE VAEKGDVGLV AENSRSTQRL MLPPPPLTIS
410 420 430 440 450
GVFTKFCDIA RLTGSASMAK KMDIIKGLFV ACRHSEARYI ARSLSGRLRL
460 470 480 490 500
GLAEQSVLAA LAQAVSLTPP GQEFPTVVVD AGKGKTAEAR KMWLEEQGMI
510 520 530 540 550
LKQTFCEVPD LDRIIPVLLE HGLERLPEHC KLSPGVPLKP MLAHPTRGVS
560 570 580 590 600
EVLKRFEEVD FTCEYKYDGQ RAQIHVLEGG EVKIFSRNQE DNTGKYPDII
610 620 630 640 650
SRIPKIKHPS VTSFILDTEA VAWDREKKQI QPFQVLTTRK RKEVDASEIQ
660 670 680 690 700
VQVCLYAFDL IYLNGESLVR QPLSRRRQLL RENFVETEGE FVFTTSLDTK
710 720 730 740 750
DTEQIAEFLE QSVKDSCEGL MVKTLDVDAT YEIAKRSHNW LKLKKDYLDG
760 770 780 790 800
VGDTLDLVVI GAYLGRGKRA GRYGGFLLAA YDEESEELQA ICKLGTGFSD
810 820 830 840 850
EELEEHHQSL QALVLPTPRP YVRIDGAVAP DHWLDPSIVW EVKCADLSLS
860 870 880 890 900
PIYPAARGLV DKEKGISLRF PRFIRVRKDK QPEQATTSNQ VASLYRKQSQ
910
IQNQQSSDLD SDVEDY
Length:916
Mass (Da):102,290
Last modified:February 1, 1996 - v2
Checksum:i6310B044364FB0E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04674 mRNA. Translation: AAA70403.1.
U19604 mRNA. Translation: AAB60500.1.
PIRiI48921.
RefSeqiNP_034845.2. NM_010715.2.
UniGeneiMm.288179.
Mm.421129.

Genome annotation databases

GeneIDi16881.
KEGGimmu:16881.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04674 mRNA. Translation: AAA70403.1.
U19604 mRNA. Translation: AAB60500.1.
PIRiI48921.
RefSeqiNP_034845.2. NM_010715.2.
UniGeneiMm.288179.
Mm.421129.

3D structure databases

ProteinModelPortaliP37913.
SMRiP37913.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201164. 2 interactors.
STRINGi10090.ENSMUSP00000096411.

PTM databases

iPTMnetiP37913.
PhosphoSitePlusiP37913.
SwissPalmiP37913.

Proteomic databases

EPDiP37913.
MaxQBiP37913.
PaxDbiP37913.
PeptideAtlasiP37913.
PRIDEiP37913.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi16881.
KEGGimmu:16881.

Organism-specific databases

CTDi3978.
MGIiMGI:101789. Lig1.

Phylogenomic databases

eggNOGiKOG0967. Eukaryota.
COG1793. LUCA.
HOVERGENiHBG005514.
InParanoidiP37913.
KOiK10747.

Miscellaneous databases

PROiP37913.
SOURCEiSearch...

Gene expression databases

CleanExiMM_LIG1.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNLI1_MOUSE
AccessioniPrimary (citable) accession number: P37913
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.