Reviewed, UniProtKB/Swiss-Prot P37913 (DNLI1_MOUSE)

Names and origin

Protein names

Recommended name:
    DNA ligase 1
    EC=6.5.1.1
Alternative name(s):
    DNA ligase I
    Polydeoxyribonucleotide synthase [ATP] 1

Gene names

Name:	Lig1
Synonyms:	Lig-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	916 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from mutant phenotype. Source: MGI cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 916	916	DNA ligase 1		PRO_0000059571
Regions
	Region	447 – 456	10	Interaction with target DNA By similarity
	Region	640 – 642	3	Interaction with target DNA By similarity
Sites
	Active site	566	1	N6-AMP-lysine intermediate By similarity
	Metal binding	619	1	Magnesium 1 By similarity
	Metal binding	718	1	Magnesium 2 By similarity
	Binding site	564	1	ATP By similarity
	Binding site	571	1	ATP By similarity
	Binding site	587	1	ATP By similarity
	Binding site	723	1	ATP By similarity
	Binding site	736	1	ATP By similarity
	Binding site	742	1	ATP By similarity
	Site	303	1	Interaction with target DNA By similarity
	Site	588	1	Interaction with target DNA By similarity
	Site	768	1	Interaction with target DNA By similarity
	Site	793	1	Interaction with target DNA By similarity
Amino acid modifications
	Modified residue	49	1	Phosphoserine By similarity
	Modified residue	51	1	Phosphoserine
	Modified residue	77	1	Phosphothreonine
	Modified residue	183	1	Phosphoserine Ref.4
	Modified residue	188	1	Phosphoserine Ref.4
	Modified residue	193	1	Phosphothreonine By similarity
	Modified residue	229	1	Phosphoserine By similarity
	Modified residue	232	1	Phosphothreonine By similarity
	Modified residue	911	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37913-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 6310B044364FB0E7	FASTA	916	102,290
	10 20 30 40 50 60 MQRSIMSFFQ PTKEGKAKKP EKETPSSIRE KEPPPKVALK ERNQVVPESD SPVKRTGRKV 70 80 90 100 110 120 AQVLSCEGED EDEAPGTPKV QKPVSDSEQS SPPSPDTCPE NSPVFNCSSP MDISPSGFPK 130 140 150 160 170 180 RRTARKQLPK RTIQDTLEEQ NEDKTKTAKK RKKEEETPKE SLAEAEDIKQ KEEKEGDQLI 190 200 210 220 230 240 VPSEPTKSPE SVTLTKTENI PVCKAGVKLK PQEEEQSKPP ARGAKTLSSF FTPRKPAVKT 250 260 270 280 290 300 EVKQEESGTL RKEETKGTLD PANYNPSKNN YHPIEDACWK HGQKVPFLAV ARTFEKIEEV 310 320 330 340 350 360 SARLKMVETL SNLLRSVVAL SPPDLLPVLY LSLNRLGPPQ QGLELGVGDG VLLKAVAQAT 370 380 390 400 410 420 GRQLESIRAE VAEKGDVGLV AENSRSTQRL MLPPPPLTIS GVFTKFCDIA RLTGSASMAK 430 440 450 460 470 480 KMDIIKGLFV ACRHSEARYI ARSLSGRLRL GLAEQSVLAA LAQAVSLTPP GQEFPTVVVD 490 500 510 520 530 540 AGKGKTAEAR KMWLEEQGMI LKQTFCEVPD LDRIIPVLLE HGLERLPEHC KLSPGVPLKP 550 560 570 580 590 600 MLAHPTRGVS EVLKRFEEVD FTCEYKYDGQ RAQIHVLEGG EVKIFSRNQE DNTGKYPDII 610 620 630 640 650 660 SRIPKIKHPS VTSFILDTEA VAWDREKKQI QPFQVLTTRK RKEVDASEIQ VQVCLYAFDL 670 680 690 700 710 720 IYLNGESLVR QPLSRRRQLL RENFVETEGE FVFTTSLDTK DTEQIAEFLE QSVKDSCEGL 730 740 750 760 770 780 MVKTLDVDAT YEIAKRSHNW LKLKKDYLDG VGDTLDLVVI GAYLGRGKRA GRYGGFLLAA 790 800 810 820 830 840 YDEESEELQA ICKLGTGFSD EELEEHHQSL QALVLPTPRP YVRIDGAVAP DHWLDPSIVW 850 860 870 880 890 900 EVKCADLSLS PIYPAARGLV DKEKGISLRF PRFIRVRKDK QPEQATTSNQ VASLYRKQSQ 910 IQNQQSSDLD SDVEDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of a cDNA coding for the murine DNA ligase I enzyme." Savini E., Biamonti G., Ciarrocchi G., Montecucco A. Gene 144:253-257(1994) [PubMed: 8039710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fibroblast.
[2]	Montecucco A. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Comparison between cDNA clones encoding murine DNA ligase I." Jessop J.K., Melton D.W. Gene 160:307-308(1995) [PubMed: 7642116] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/Ola.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183 AND SER-188, MASS SPECTROMETRY. Tissue: Liver.
[5]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-77, MASS SPECTROMETRY. Tissue: Macrophage.
[6]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U04674 mRNA. Translation: AAA70403.1. U19604 mRNA. Translation: AAB60500.1.
IPI	IPI00473314.
PIR	I48921.
RefSeq	NP_034845.2.
UniGene	Mm.288179 Mm.421129
3D structure databases
SMR	P37913. Positions 260-899.
ModBase	Search...
PTM databases
PhosphoSite	P37913.
Genome annotation databases
Ensembl	ENSMUSG00000056394. Mus musculus. [Contig view]
GeneID	16881.
Organism-specific databases
MGI	MGI:101789. Lig1.
Phylogenomic databases
HOVERGEN	P37913.
Enzyme and pathway databases
BRENDA	6.5.1.1. 244.
Gene expression databases
ArrayExpress	P37913.
Bgee	P37913.
CleanEx	MM_LIG1.
GermOnline	ENSMUSG00000056394. Mus musculus.
Family and domain databases
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	290882.
SOURCE	Search...

Entry information

Entry name

DNLI1_MOUSE

Accession

Primary (citable) accession number: P37913

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)