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Protein

Gamma-glutamylputrescine oxidoreductase

Gene

puuB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine.1 Publication

Catalytic activityi

Gamma-glutamylputrescine + H2O + O2 = Gamma-glutamyl-gamma-aminobutyraldehyde + NH3 + H2O2.

Pathwayi: putrescine degradation

This protein is involved in step 2 of the subpathway that synthesizes 4-aminobutanoate from putrescine.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Gamma-glutamylputrescine synthetase PuuA (puuA)
  2. Gamma-glutamylputrescine oxidoreductase (puuB)
  3. Aldehyde dehydrogenase PuuC (puuC)
  4. Gamma-glutamyl-gamma-aminobutyrate hydrolase PuuD (puuD)
This subpathway is part of the pathway putrescine degradation, which is itself part of Amine and polyamine degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-aminobutanoate from putrescine, the pathway putrescine degradation and in Amine and polyamine degradation.

GO - Molecular functioni

  • oxidoreductase activity Source: UniProtKB

GO - Biological processi

  • oxidation-reduction process Source: UniProtKB
  • putrescine catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:EG11822-MONOMER.
ECOL316407:JW1294-MONOMER.
MetaCyc:EG11822-MONOMER.
BRENDAi1.4.3.B1. 2026.
UniPathwayiUPA00188; UER00881.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamylputrescine oxidoreductase (EC:1.4.3.-)
Short name:
Gamma-Glu-Put oxidase
Short name:
Gamma-glutamylputrescine oxidase
Gene namesi
Name:puuB
Synonyms:ordL, ycjA
Ordered Locus Names:b1301, JW1294
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11822. puuB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000971091 – 426Gamma-glutamylputrescine oxidoreductaseAdd BLAST426

Proteomic databases

PaxDbiP37906.
PRIDEiP37906.

Interactioni

Protein-protein interaction databases

BioGridi4263527. 12 interactors.
IntActiP37906. 18 interactors.
STRINGi511145.b1301.

Structurei

3D structure databases

ProteinModelPortaliP37906.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CJP. Bacteria.
ENOG410XWBT. LUCA.
HOGENOMiHOG000185563.
InParanoidiP37906.
KOiK09471.
OMAiIECDYRP.
PhylomeDBiP37906.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

P37906-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEHTSSYYA ASANKYAPFD TLNESITCDV CVVGGGYTGL SSALHLAEAG
60 70 80 90 100
FDVVVLEASR IGFGASGRNG GQLVNSYSRD IDVIEKSYGM DTARMLGSMM
110 120 130 140 150
FEGGEIIRER IKRYQIDCDY RPGGLFVAMN DKQLATLEEQ KENWERYGNK
160 170 180 190 200
QLELLDANAI RREVASDRYT GALLDHSGGH IHPLNLAIGE ADAIRLNGGR
210 220 230 240 250
VYELSAVTQI QHTTPAVVRT AKGQVTAKYV IVAGNAYLGD KVEPELAKRS
260 270 280 290 300
MPCGTQVITT ERLSEDLARS LIPKNYCVED CNYLLDYYRL TADNRLLYGG
310 320 330 340 350
GVVYGARDPD DVERLVVPKL LKTFPQLKGV KIDYRWTGNF LLTLSRMPQF
360 370 380 390 400
GRLDTNIYYM QGYSGHGVTC THLAGRLIAE LLRGDAERFD AFANLPHYPF
410 420
PGGRTLRVPF TAMGAAYYSL RDRLGV
Length:426
Mass (Da):47,170
Last modified:October 1, 1996 - v2
Checksum:i44158FF418E9C254
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38543 Genomic DNA. Translation: AAC45300.1.
AB200320 Genomic DNA. Translation: BAD88709.1.
U00096 Genomic DNA. Translation: AAC74383.1.
AP009048 Genomic DNA. Translation: BAA14870.1.
M38433 Genomic DNA. Translation: AAA23429.1.
PIRiH64878.
RefSeqiNP_415817.1. NC_000913.3.
WP_000134870.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74383; AAC74383; b1301.
BAA14870; BAA14870; BAA14870.
GeneIDi945072.
KEGGiecj:JW1294.
eco:b1301.
PATRICi32117874. VBIEscCol129921_1357.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38543 Genomic DNA. Translation: AAC45300.1.
AB200320 Genomic DNA. Translation: BAD88709.1.
U00096 Genomic DNA. Translation: AAC74383.1.
AP009048 Genomic DNA. Translation: BAA14870.1.
M38433 Genomic DNA. Translation: AAA23429.1.
PIRiH64878.
RefSeqiNP_415817.1. NC_000913.3.
WP_000134870.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37906.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263527. 12 interactors.
IntActiP37906. 18 interactors.
STRINGi511145.b1301.

Proteomic databases

PaxDbiP37906.
PRIDEiP37906.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74383; AAC74383; b1301.
BAA14870; BAA14870; BAA14870.
GeneIDi945072.
KEGGiecj:JW1294.
eco:b1301.
PATRICi32117874. VBIEscCol129921_1357.

Organism-specific databases

EchoBASEiEB1769.
EcoGeneiEG11822. puuB.

Phylogenomic databases

eggNOGiENOG4105CJP. Bacteria.
ENOG410XWBT. LUCA.
HOGENOMiHOG000185563.
InParanoidiP37906.
KOiK09471.
OMAiIECDYRP.
PhylomeDBiP37906.

Enzyme and pathway databases

UniPathwayiUPA00188; UER00881.
BioCyciEcoCyc:EG11822-MONOMER.
ECOL316407:JW1294-MONOMER.
MetaCyc:EG11822-MONOMER.
BRENDAi1.4.3.B1. 2026.

Miscellaneous databases

PROiP37906.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPUUB_ECOLI
AccessioniPrimary (citable) accession number: P37906
Secondary accession number(s): Q5H773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.