P37902 (GLTI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate/aspartate periplasmic-binding protein

Gene names

Name:	gltI
Synonyms:	ybeJ, yzzK
Ordered Locus Names:	b0655, JW5092

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the binding-protein-dependent transport system for glutamate and aspartate. Binds to both aspartate and glutamate.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the bacterial solute-binding protein 3 family.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Periplasm
Domain	Signal
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.6
	Chain	23 – 302	280	Glutamate/aspartate periplasmic-binding protein		PRO_0000031758

Sequences

	Sequence		Length	Mass (Da)
	P37902 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 6FFC3321040152EC	FASTA	302	33,420
	10 20 30 40 50 60 MQLRKPATAI LALALSAGLA QADDAAPAAG STLDKIAKNG VIVVGHRESS VPFSYYDNQQ 70 80 90 100 110 120 KVVGYSQDYS NAIVEAVKKK LNKPDLQVKL IPITSQNRIP LLQNGTFDFE CGSTTNNVER 130 140 150 160 170 180 QKQAAFSDTI FVVGTRLLTK KGGDIKDFAN LKDKAVVVTS GTTSEVLLNK LNEEQKMNMR 190 200 210 220 230 240 IISAKDHGDS FRTLESGRAV AFMMDDALLA GERAKAKKPD NWEIVGKPQS QEAYGCMLRK 250 260 270 280 290 300 DDPQFKKLMD DTIAQVQTSG EAEKWFDKWF KNPIPPKNLN MNFELSDEMK ALFKEPNDKA LN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Sequence and characterisation of three genes of a glutamate-aspartate binding protein-dependent transport system of Escherichia coli K12." Lum D., Wallace B.J. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 242-302. Strain: K12 / BK9MDG.
[6]	Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F. Submitted (SEP-1994) to UniProtKB Cited for: PROTEIN SEQUENCE OF 23-33. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B. Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: B / BL21.
[8]	"Purification and properties of a periplasmic glutamate-aspartate binding protein from Escherichia coli K12 strain W3092." Willis R.C., Furlong C.E. J. Biol. Chem. 250:2574-2580(1975) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / W3092.
[9]	"Interactions of a glutamate-aspartate binding protein with the glutamate transport system of Escherichia coli." Willis R.C., Furlong C.E. J. Biol. Chem. 250:2581-2586(1975) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82598 Genomic DNA. Translation: AAB40856.1. U00096 Genomic DNA. Translation: AAC73756.1. AP009048 Genomic DNA. Translation: BAA35307.2. U10981 Genomic DNA. No translation available.
PIR	E64800.
RefSeq	NP_415188.1. NC_000913.2. YP_488946.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P37902.
SMR	P37902. Positions 25-302.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11361N.
STRING	511145.b0655.
Protein family/group databases
TCDB	3.A.1.3.4. ATP-binding cassette (ABC) superfamily.
2D gel databases
SWISS-2DPAGE	P37902.
Proteomic databases
PaxDb	P37902.
PRIDE	P37902.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73756; AAC73756; b0655. BAA35307; BAA35307; BAA35307.
GeneID	12931835. 946938.
KEGG	ecj:Y75_p0645. eco:b0655.
PATRIC	32116493. VBIEscCol129921_0686.
Organism-specific databases
EchoBASE	EB2562.
EcoGene	EG12700. gltI.
Phylogenomic databases
eggNOG	COG0834.
HOGENOM	HOG000031898.
KO	K10001.
OMA	IISTKDH.
ProtClustDB	PRK10797.
Enzyme and pathway databases
BioCyc	EcoCyc:G6359-MONOMER. ECOL316407:JW5092-MONOMER.
Gene expression databases
Genevestigator	P37902.
Family and domain databases
InterPro	IPR001638. SBP_bac_3. [Graphical view]
Pfam	PF00497. SBP_bac_3. 1 hit. [Graphical view]
SMART	SM00062. PBPb. 1 hit. [Graphical view]
PROSITE	PS01039. SBP_BACTERIAL_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLTI_ECOLI

Accession

Primary (citable) accession number: P37902
Secondary accession number(s): P41408 , P77612, Q9R7T1, Q9R7T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program