Reviewed, UniProtKB/Swiss-Prot P37902 (GLTI_ECOLI)

Names and origin

Protein names

Recommended name:
Glutamate/aspartate periplasmic-binding protein

Gene names

Name:	gltI
Synonyms:	ybeJ, yzzK
Ordered Locus Names:	b0655, JW5092

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Part of the binding-protein-dependent transport system for glutamate and aspartate. Binds to both aspartate and glutamate.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the bacterial solute-binding protein 3 family.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Periplasm
Domain	Signal
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.6
	Chain	23 – 302	280	Glutamate/aspartate periplasmic-binding protein		PRO_0000031758

Sequences

	Sequence		Length	Mass (Da)
	P37902-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 6FFC3321040152EC	FASTA	302	33,420
	10 20 30 40 50 60 MQLRKPATAI LALALSAGLA QADDAAPAAG STLDKIAKNG VIVVGHRESS VPFSYYDNQQ 70 80 90 100 110 120 KVVGYSQDYS NAIVEAVKKK LNKPDLQVKL IPITSQNRIP LLQNGTFDFE CGSTTNNVER 130 140 150 160 170 180 QKQAAFSDTI FVVGTRLLTK KGGDIKDFAN LKDKAVVVTS GTTSEVLLNK LNEEQKMNMR 190 200 210 220 230 240 IISAKDHGDS FRTLESGRAV AFMMDDALLA GERAKAKKPD NWEIVGKPQS QEAYGCMLRK 250 260 270 280 290 300 DDPQFKKLMD DTIAQVQTSG EAEKWFDKWF KNPIPPKNLN MNFELSDEMK ALFKEPNDKA LN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Sequence and characterisation of three genes of a glutamate-aspartate binding protein-dependent transport system of Escherichia coli K12." Lum D., Wallace B.J. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 242-302. Strain: K12 / BK9MDG.
[6]	Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F. Submitted (SEP-1994) to UniProtKB Cited for: PROTEIN SEQUENCE OF 23-33. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B. Electrophoresis 20:2181-2195(1999) [PubMed: 10493123] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[8]	"Purification and properties of a periplasmic glutamate-aspartate binding protein from Escherichia coli K12 strain W3092." Willis R.C., Furlong C.E. J. Biol. Chem. 250:2574-2580(1975) [PubMed: 1091635] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / W3092.
[9]	"Interactions of a glutamate-aspartate binding protein with the glutamate transport system of Escherichia coli." Willis R.C., Furlong C.E. J. Biol. Chem. 250:2581-2586(1975) [PubMed: 1091636] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82598 Genomic DNA. Translation: AAB40856.1. U00096 Genomic DNA. Translation: AAC73756.1. AP009048 Genomic DNA. Translation: BAA35307.2. U10981 Genomic DNA. No translation available.
PIR	E64800.
RefSeq	AP_001304.1. NP_415188.1.
3D structure databases
SMR	P37902. Positions 25-302.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11361N.
STRING	P37902.
Protein family/group databases
TCDB	3.A.1.3.4. ATP-binding cassette (ABC) superfamily.
2-D gel databases
SWISS-2DPAGE	P37902.
2DBase-Ecoli	P37902.
Genome annotation databases
GeneID	946938.
GenomeReviews	Gene locus JW5092 in contig AP009048_GR. Gene locus b0655 in contig U00096_GR.
KEGG	ecj:JW5092. eco:b0655.
Organism-specific databases
EchoBASE	EB2562.
EcoGene	EG12700. gltI.
CMR	Search...
Phylogenomic databases
eggNOG	COG0834.
HOGENOM	HBG698334.
OMA	PFSYIAD.
ProtClustDB	PRK10797.
Enzyme and pathway databases
BioCyc	EcoCyc:G6359-MONOMER. ECOL168927:B0655-MONOMER.
Gene expression databases
Genevestigator	P37902.
Family and domain databases
InterPro	IPR015683. Glutamate_receptor-rel. IPR001638. SBP_bac_3. [Graphical view]
PANTHER	PTHR18966. Glut_Rec_Related. 1 hit.
Pfam	PF00497. SBP_bac_3. 1 hit. [Graphical view]
SMART	SM00062. PBPb. 1 hit. [Graphical view]
PROSITE	PS01039. SBP_BACTERIAL_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLTI_ECOLI

Accession

Primary (citable) accession number: P37902
Secondary accession number(s): P41408 , P77612, Q9R7T1, Q9R7T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 1, 1997
Last modified:	March 2, 2010
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)