P37898 (AAP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine/arginine aminopeptidase
EC=3.4.11.-

Gene names

Name:	AAP1
Ordered Locus Names:	YHR047C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	856 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positive effector of glycogen accumulation. May be involved in nutrient-sensing.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Miscellaneous	Present with 77000 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen metabolic process Inferred from mutant phenotype Ref.1. Source: SGD proteolysis Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	aminopeptidase activity Inferred from sequence or structural similarity Ref.1. Source: SGD metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 856	856	Alanine/arginine aminopeptidase		PRO_0000095105
Regions
	Region	264 – 268	5	Substrate binding By similarity
Sites
	Active site	301	1	Proton acceptor By similarity
	Metal binding	300	1	Zinc; catalytic By similarity
	Metal binding	304	1	Zinc; catalytic By similarity
	Metal binding	323	1	Zinc; catalytic By similarity
	Binding site	132	1	Substrate By similarity
	Site	386	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	248	1	Phosphotyrosine Ref.5
Experimental info
	Sequence conflict	550 – 569	20	Missing Ref.2
	Sequence conflict	646	1	V → E Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P37898 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 0209F9E0298DF9DB	FASTA	856	97,663
	10 20 30 40 50 60 MSREVLPNNV TPLHYDITLE PNFRAFTFEG SLKIDLQIND HSINSVQINY LEIDFHSARI 70 80 90 100 110 120 EGVNAIEVNK NENQQKATLV FPNGTFENLG PSAKLEIIFS GILNDQMAGF YRAKYTDKVT 130 140 150 160 170 180 GETKYMATTQ MEATDARRAF PCFDEPNLKA TFAVTLVSES FLTHLSNMDV RNETIKEGKK 190 200 210 220 230 240 YTTFNTTPKM STYLVAFIVA DLRYVESNNF RIPVRVYSTP GDEKFGQFAA NLAARTLRFF 250 260 270 280 290 300 EDTFNIEYPL PKMDMVAVHE FSAGAMENWG LVTYRVIDLL LDIENSSLDR IQRVAEVIQH 310 320 330 340 350 360 ELAHQWFGNL VTMDWWEGLW LNEGFATWMS WYSCNKFQPE WKVWEQYVTD NLQRALNLDS 370 380 390 400 410 420 LRSSHPIEVP VNNADEINQI FDAISYSKGS SLLRMISKWL GEETFIKGVS QYLNKFKYGN 430 440 450 460 470 480 AKTGDLWDAL ADASGKDVCS VMNIWTKRVG FPVLSVKEHK NKITLTQHRY LSTGDVKEEE 490 500 510 520 530 540 DTTIYPILLA LKDSTGIDNT LVLNEKSATF ELKNEEFFKI NGDQSGIFIT SYSDERWAKL 550 560 570 580 590 600 SKQANLLSVE DRVGLVADAK ALSASGYTST TNFLNLISNW KNEDSFVVWE QIINSLSALK 610 620 630 640 650 660 STWVFEPEDI LNALDKFTLD LVLNKLSELG WNIGEDDSFA IQRLKVTLFS AACTSGNEKM 670 680 690 700 710 720 QSIAVEMFEE YANGNKQAIP ALFKAVVFNT VARLGGENNY EKIFNIYQNP VSSEEKIIAL 730 740 750 760 770 780 RALGRFEDKE LLERTLSYLL DGTVLNQDFY IPMQGIRVHK KGIERLWAWM QEHWDEIAKR 790 800 810 820 830 840 LQPGSPVLGG VLTLGLTNFT SFEALEKISA FYSRKVTKGF DQTLAQALDT IRSKAQWVSR 850 DREIVATYLR EHEYDQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of AAP1. A gene encoding an alanine/arginine aminopeptidase in yeast." Caprioglio D.R., Padilla C., Werner-Washburne M. J. Biol. Chem. 268:14310-14315(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-652.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-248, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12542 Genomic DNA. No translation available. U00062 Genomic DNA. Translation: AAB68919.1. BK006934 Genomic DNA. Translation: DAA06739.1.
PIR	S46750.
RefSeq	NP_011913.1. NM_001179177.1.
3D structure databases
ProteinModelPortal	P37898.
SMR	P37898. Positions 2-852.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6698N.
IntAct	P37898. 8 interactions.
MINT	MINT-2786533.
STRING	4932.YHR047C.
Protein family/group databases
MEROPS	M01.007.
Proteomic databases
PaxDb	P37898.
PeptideAtlas	P37898.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR047C; YHR047C; YHR047C.
GeneID	856443.
KEGG	sce:YHR047C.
Organism-specific databases
CYGD	YHR047c.
SGD	S000001089. AAP1.
Phylogenomic databases
eggNOG	COG0308.
GeneTree	ENSGT00680000099758.
HOGENOM	HOG000106482.
OMA	MISHEIS.
OrthoDB	EOG42VCQG.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31102-MONOMER.
Gene expression databases
Genevestigator	P37898.
GermOnline	YHR047C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024571. DUF3358. IPR001930. Peptidase_M1. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. PTHR11533. 1 hit.
Pfam	PF11838. DUF3358. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982057.

Entry information

Entry name

AAP1_YEAST

Accession

Primary (citable) accession number: P37898
Secondary accession number(s): D3DKZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program