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P37896

- AMPN_LACDL

UniProt

P37896 - AMPN_LACDL

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Protein

Aminopeptidase N

Gene

pepN

Organism
Lactobacillus delbrueckii subsp. lactis
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Aminopeptidase with broad substrate specificity to several peptides.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

pH dependencei

Optimum pH is 6.5-7.0.

Temperature dependencei

Optimum temperature is 54-55 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201SubstrateBy similarity
Metal bindingi288 – 2881Zinc; catalyticPROSITE-ProRule annotation
Active sitei289 – 2891Proton acceptorPROSITE-ProRule annotation
Metal bindingi292 – 2921Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi311 – 3111Zinc; catalyticPROSITE-ProRule annotation
Sitei375 – 3751Transition state stabilizerBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name:
Lys-AP
Gene namesi
Name:pepN
OrganismiLactobacillus delbrueckii subsp. lactis
Taxonomic identifieri29397 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

Cytoplasm
Note: It may be secreted through an unknown mechanism.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 843842Aminopeptidase NPRO_0000095071Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP37896.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni252 – 2565Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37896-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVKRFYETF HPDHYDLYID VDRAARSFSG TSTIHGEIQE ETVLVHQKYM
60 70 80 90 100
TISKVTVDGK EVPFTFGDDF EGIKIEAGKT GEAVIAIDYS APLTDTMMGI
110 120 130 140 150
YPSYYQVDGV KKELIGTQFE TTFAREAFPC VDEPEAKATF SLALKFDEHE
160 170 180 190 200
GETVLANMPE DRVENGVHYF KETVRMSSYL VAFAFGEMRS LTTHTKSGVL
210 220 230 240 250
IGVYSTQAHT EKELTFSLDI AKRAIEFYED FYQTPYPLPQ SLQLALPDFS
260 270 280 290 300
AGAMENWGLV TYREAYLLLD PDNTTLEMKK LVATVVTHEL AHQWFGDLVT
310 320 330 340 350
MEWWDNLWLN ESFANMMEYL SVDHLEPNWH IWEMFQTSEA AAALTRDATD
360 370 380 390 400
GVQSVHVEVN DPAEIDALFD GAIVYAKGSR MLVMVRSLLG DEALRKGLKR
410 420 430 440 450
YFDKHKFGNA AGDDLWDALS TATDLNIGEI MHTWLDQPGY PVVNAFVEDG
460 470 480 490 500
HLKLTQKQFF IGEGKEVGRK WEIPLNANFK APKIMSDVEL DLGDYQALRA
510 520 530 540 550
EAGHALRLNV GNNSHFIVKY DQTLMDDIMK EAKDLDPVSQ LQLLQDLRLL
560 570 580 590 600
AEGKQASYAD VVPVLELFKN SESHIVNDAL YTTADKLRQF APAGSEADKN
610 620 630 640 650
LRALYNDLSK DQVARLGWLP KAGESDEDIQ TRPYVLSASL YGRNADSEKQ
660 670 680 690 700
AHEIYVEYAD KLAELSADIR PYVLINEVEN YGSSELTDKL IGLYQATSDP
710 720 730 740 750
SFKMDLEAAI VKSKDEGELK KIVSWFKNAE IVKPQDLRGW FSGVLSNPAG
760 770 780 790 800
EQLAWDWIRD EWAWLEKTVG GDMEFATFIT VISRVFKTKE RYDEYNAFFT
810 820 830 840
DKESNMLLNR EIKMDRKVIA NRVDLIASEQ ADVNAAVAAA LQK
Length:843
Mass (Da):95,348
Last modified:January 23, 2007 - v3
Checksum:i08EF9F8CBD7AB1B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21701 Genomic DNA. Translation: CAA79805.1.
PIRiS38364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21701 Genomic DNA. Translation: CAA79805.1 .
PIRi S38364.

3D structure databases

ProteinModelPortali P37896.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M01.002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, DNA sequence analysis and partial characterization of pepN, a lysyl aminopeptidase from Lactobacillus delbruckii ssp. lactis DSM7290."
    Klein J.R., Klein U., Schad M., Plapp R.
    Eur. J. Biochem. 217:105-114(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 7290.

Entry informationi

Entry nameiAMPN_LACDL
AccessioniPrimary (citable) accession number: P37896
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3