Aminopeptidase N - Caulobacter crescentus

Preferred order of sections

Names and origin

Protein names

Recommended name:
Aminopeptidase N
EC=3.4.11.2

Alternative name(s):
Alpha-aminoacylpeptide hydrolase

Gene names

Name:	pepN
Ordered Locus Names:	CC_2481

Organism

Caulobacter crescentus (Caulobacter vibrioides)

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	863 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Aminopeptidase N is involved in the degradation of intracellular peptides generated by protein breakdown during normal growth as well as in response to nutrient starvation.
Catalytic activity	Release of an N-terminal amino acid, Xaa-\|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 863

863

Aminopeptidase N

PRO_0000095068

Regions

Region

263 – 267

5

Substrate binding By similarity

Sites

Active site

300

1

Proton acceptor By similarity

Metal binding

299

1

Zinc; catalytic By similarity

Metal binding

303

1

Zinc; catalytic By similarity

Metal binding

322

1

Zinc; catalytic By similarity

Binding site

124

1

Substrate By similarity

Site

383

1

Transition state stabilizer By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P37893 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: F04BCE19C6A5F7BD
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FASTA

863

94,880

        10         20         30         40         50         60 
MRTDTPQAVN LADYRPFPFA IETTRLVFDL HPTRTRVSAE LSVRRTGGKN EPLVLNGERL 

        70         80         90        100        110        120 
KLVSIAIDGR PLAAGEYGVD AERLTIAEAP DAFVLTTEVE IDPSSNKALM GLYMSGGRFC 

       130        140        150        160        170        180 
TQCEAEGFRT ITYFPDRPDV LSRYSVRIEA DGKFPHLLSN GNPVASGSLD GGRHFAEWSD 

       190        200        210        220        230        240 
PFPKPSYLFA LVAGDLDVLA DKFITMSGRE VALRVFVDPG QASRAAYALD SLKRAMKWDE 

       250        260        270        280        290        300 
EAFGREYDLD LFMIVAVRDF NFGAMENKGL NIFNSSLLLA DPQTATDLDY ERIEAVVAHE 

       310        320        330        340        350        360 
YFHNWTGNRI TCRDWFQLCL KEGFTVFRDQ GLSADMRGAA VQRIKDVRAL RARQFAEDAG 

       370        380        390        400        410        420 
PLAHPVRPSS YLKIDNFYTA TIYEKGAEII RMLKAILGAP AFRKGCDLYF QRHDGEATTV 

       430        440        450        460        470        480 
EAFIACFAEA SGRDLSGFFG WYEQAGTPSV TIETAYDAAA GALTLTLTQS TSPTPGQPDK 

       490        500        510        520        530        540 
KPLPIPIAIG LLAADGRVLR DTEIVLLDQA QMTVRWDSIP EPPVLSALRG FSAPVNLSTD 

       550        560        570        580        590        600 
ARPSDRYVLF GSDTDLFNRW EAGQTLARDL ILTRAAGAPD EVGEERYADA LGRALVDDAA 

       610        620        630        640        650        660 
EPAFKALLLA LPSEPDLALM FEAADPAALH AARDHLRTRI AVHLGDLLRR LHGEMQINGE 

       670        680        690        700        710        720 
FSSDAAAAGR RALRNACAEA LSADPHAENL ARLLGHFGAA RNMTDMIGGL YPMVAMGGVP 

       730        740        750        760        770        780 
REKALESFHH AWRTEPLVLD KWFAVQGRDP NPDALERVIA LTQHPDFEPT NPNRLRALVS 

       790        800        810        820        830        840 
TFANFNPARF HDPSGAGYAF LADEILKVDA FNPMTAARLV EPLGGWRRYK PELGDLMRAQ 

       850        860 
LERIVAHPNL SKNVLELASK ALG

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.
[2]	"A histidine protein kinase is involved in polar organelle development in Caulobacter crescentus." Wang S.P., Sharma P.L., Schoenlein P.V., Ely B. Proc. Natl. Acad. Sci. U.S.A. 90:630-634(1993) [PubMed: 8421698] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 725-863. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005673 Genomic DNA. Translation: AAK24452.1. M91449 Genomic DNA. Translation: AAA23051.1.
PIR	H87556. S27532.
RefSeq	NP_421284.1. NC_002696.2.
3D structure databases
ProteinModelPortal	P37893.
ModBase	Search...
Protein family/group databases
MEROPS	M01.005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	944052.
GenomeReviews	Gene locus CC_2481 in contig AE005673_GR.
KEGG	ccr:CC_2481.
NMPDR	fig\|190650.1.peg.2464.
PATRIC	21301996. VBICauCre124313_2499.
TIGR	CC_2481.
Phylogenomic databases
HOGENOM	HBG289207.
OMA	DIFMIVA.
ProtClustDB	PRK14015.
Family and domain databases
InterPro	IPR001930. Peptidase_M1. IPR014782. Peptidase_M1_N. IPR012779. Peptidase_M1_pepN. IPR024601. Peptidase_M1_pepN_C. [Graphical view]
Gene3D	G3DSA:1.25.50.10. G3DSA:1.25.50.10. 1 hit.
KO	K01256.
PANTHER	PTHR11533:SF8. Pept_M1_pepN. 1 hit. PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF11940. DUF3458. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
TIGRFAMs	TIGR02414. PepN_proteo. 1 hit.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPN_CAUCR

Accession

Primary (citable) accession number: P37893

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents