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Protein

Cysteine synthase

Gene

cysK

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the formation of a CymR-CysK complex and transcriptional repression of the CymR regulon occurs. In the absence of cysteine, the OAS pool is high and the CymR-CysK complex is mostly dissociated, leading to a faster dissociation of CymR from its DNA targets and the lifting of CymR-dependent repression.1 Publication

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.1 Publication

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serine acetyltransferase (cysE)
  2. Probable cysteine synthase (ytkP), Cysteine synthase (cysK)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75Pyridoxal phosphateBy similarity1
Binding sitei267Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciBSUB:BSU00730-MONOMER.
UniPathwayiUPA00136; UER00200.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine synthase (EC:2.5.1.47)
Short name:
CSase
Alternative name(s):
O-acetylserine (thiol)-lyase
Short name:
OAS-TL
Superoxide-inducible protein 11
Short name:
SOI11
Gene namesi
Name:cysK
Ordered Locus Names:BSU00730
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Grows very slowly with sulfate, butanesulfonate or cystine as sole sulfur source.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001670812 – 308Cysteine synthaseAdd BLAST307

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP37887.
PRIDEiP37887.

Expressioni

Inductioni

Highly expressed in the presence of methionine, but poorly expressed in the presence of cystine. Also induced by superoxide. Repressed both by sulfate and cysteine.2 Publications

Interactioni

Subunit structurei

Homodimer. Forms CymR2:CysK2 or CymR4:CysK4 complexes in the absence of O-acetylserine.1 Publication

Protein-protein interaction databases

IntActiP37887. 1 interactor.
MINTiMINT-8365723.
STRINGi224308.Bsubs1_010100000370.

Structurei

3D structure databases

ProteinModelPortaliP37887.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 183Pyridoxal phosphate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C6T. Bacteria.
COG0031. LUCA.
HOGENOMiHOG000217394.
InParanoidiP37887.
KOiK01738.
OMAiVKCRIGS.
PhylomeDBiP37887.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37887-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRVANSITE LIGNTPIVKL NRLADENSAD VYLKLEYMNP GSSVKDRIGL
60 70 80 90 100
AMIEAAEKEG KLKAGNTIIE PTSGNTGIGL AMVAAAKGLK AILVMPDTMS
110 120 130 140 150
MERRNLLRAY GAELVLTPGA EGMKGAIKKA EELAEKHGYF VPQQFNNPSN
160 170 180 190 200
PEIHRQTTGK EIVEQFGDDQ LDAFVAGIGT GGTITGAGEV LKEAYPSIKI
210 220 230 240 250
YAVEPSDSPV LSGGKPGPHK IQGIGAGFVP DILNTEVYDE IFPVKNEEAF
260 270 280 290 300
EYARRAAREE GILGGISSGA AIYAALQVAK KLGKGKKVLA IIPSNGERYL

STPLYQFD
Length:308
Mass (Da):32,820
Last modified:January 23, 2007 - v3
Checksum:i79350923DF2B73CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05308.1.
AL009126 Genomic DNA. Translation: CAB11849.1.
PIRiS66103.
RefSeqiNP_387954.1. NC_000964.3.
WP_003244491.1. NZ_JNCM01000028.1.

Genome annotation databases

EnsemblBacteriaiCAB11849; CAB11849; BSU00730.
GeneIDi936636.
KEGGibsu:BSU00730.
PATRICi18971621. VBIBacSub10457_0074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05308.1.
AL009126 Genomic DNA. Translation: CAB11849.1.
PIRiS66103.
RefSeqiNP_387954.1. NC_000964.3.
WP_003244491.1. NZ_JNCM01000028.1.

3D structure databases

ProteinModelPortaliP37887.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP37887. 1 interactor.
MINTiMINT-8365723.
STRINGi224308.Bsubs1_010100000370.

Proteomic databases

PaxDbiP37887.
PRIDEiP37887.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11849; CAB11849; BSU00730.
GeneIDi936636.
KEGGibsu:BSU00730.
PATRICi18971621. VBIBacSub10457_0074.

Phylogenomic databases

eggNOGiENOG4105C6T. Bacteria.
COG0031. LUCA.
HOGENOMiHOG000217394.
InParanoidiP37887.
KOiK01738.
OMAiVKCRIGS.
PhylomeDBiP37887.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00200.
BioCyciBSUB:BSU00730-MONOMER.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSK_BACSU
AccessioniPrimary (citable) accession number: P37887
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.