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Protein

Arginine--tRNA ligase, cytoplasmic

Gene

RARS

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.By similarity

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei212L-arginineBy similarity1
Binding sitei385L-arginineBy similarity1
Binding sitei389L-arginineBy similarity1
Binding sitei413L-arginineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKiP37880.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase, cytoplasmic (EC:6.1.1.19By similarity)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:RARS
Synonyms:RRS1
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000357951 – 661Arginine--tRNA ligase, cytoplasmicAdd BLAST661

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP37880.

Interactioni

Subunit structurei

Interacts (via N-terminus) with AIMP1 (via N-terminus); this stimulates its catalytic activity. Interacts (via N-terminus) with LARS2 (via C-terminus). Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts with QARS. Part of a complex composed of RARS, QARS and AIMP1.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliP37880.
SMRiP37880.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 73Could be involved in the assembly of the multisynthetase complexAdd BLAST73
Regioni201 – 203L-arginine bindingBy similarity3
Regioni530 – 544Interaction with tRNABy similarityAdd BLAST15

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi202 – 213"HIGH" regionAdd BLAST12

Domaini

The alpha-helical N-terminus (residues 1-72) mediates interaction with AIMP1 and thereby contributes to the assembly of the multisynthetase complex.By similarity

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG029238.
KOiK01887.

Family and domain databases

CDDicd00671. ArgRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR036695. Arg-tRNA-synth_N_sf.
IPR035684. ArgRS_core.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiView protein in Pfam
PF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
PRINTSiPR01038. TRNASYNTHARG.
SMARTiView protein in SMART
SM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Complexed (identifier: P37880-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGLVAQCSA RLLQQEKEIK SLTAEIDRLK NCGHLEDSPS LDQLREENLK
60 70 80 90 100
LKYRLNILQR SLQAEKRRRP TKNMININSR LQDVFGCAIK AAYPDLDNPP
110 120 130 140 150
LVVTPSQQPK FGDYQCNSAM GISQMLKAKE QKVNPRGIAE NITKHLPNNE
160 170 180 190 200
YIDRVEIAGP GFINVHLRKD FVSEQLTNLL VNGIQLPALG ENKKVIVDFS
210 220 230 240 250
SPNIAKEMHV GHLRSTIIGE SMSRLFEFAG YDVLRLNHVG DWGTQFGMLI
260 270 280 290 300
AHLQDQFPDY LTVSPPIGDL QAFYKESKKR FDTEEEFKKR AYQCVVSLQS
310 320 330 340 350
KDPDFIKAWN LICDVSRAEF NKIYDALDIT LIERGESFYQ DRMKDIVKEF
360 370 380 390 400
EDKGYVQVDD GRKIVFVPGC SIPLTIVKSD GGFTYDTSDL AAIKQRLFEE
410 420 430 440 450
KANKIIYVVD NGQAVHFQTI FAAAQMIGWY DPKVTQVTHV GFGVVLGEDK
460 470 480 490 500
KKFKTRSGET VRLVDLLGEG LKRSMDKLKE KERDKVLTEE ELTAAQTSIA
510 520 530 540 550
YGCIKYADLS HNRLNDYIFS FDKMLDDRGN TAAYLLYAFT RIRSIARLAN
560 570 580 590 600
VDEEMLQKAA RETKIVLDHE KEWKLGRCIL RFPEILQKML DDLFLHTLCD
610 620 630 640 650
YIYELATTFT EFYDSCYCVE KDRQTGKVLK VNMWRMLLCE AVAAVMAKGF
660
DILGIKPVQR M
Length:661
Mass (Da):75,602
Last modified:October 1, 1994 - v1
Checksum:i12EB1C85655EB8F4
GO
Isoform Monomeric (identifier: P37880-2) [UniParc]FASTAAdd to basket
Also known as: Free

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.

Note: The alternative initiation site Met-74 is uncertain.
Show »
Length:588
Mass (Da):67,088
Checksum:i623A7303E0156F90
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189041 – 73Missing in isoform Monomeric. CuratedAdd BLAST73

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63415 mRNA. Translation: CAA45012.1.
RefSeqiXP_007632694.1. XM_007634504.2. [P37880-1]

Genome annotation databases

EnsembliENSCGRT00001003508; ENSCGRP00001002597; ENSCGRG00001002920.
GeneIDi100767723.
KEGGicge:100767723.

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiSYRC_CRIGR
AccessioniPrimary (citable) accession number: P37880
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 25, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families