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P37880

- SYRC_CRIGR

UniProt

P37880 - SYRC_CRIGR

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Protein

Arginine--tRNA ligase, cytoplasmic

Gene
RARS, RRS1
Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1 By similarity.UniRule annotation

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).UniRule annotation

GO - Molecular functioni

  1. arginine-tRNA ligase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. arginyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP37880.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase, cytoplasmic (EC:6.1.1.19)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:RARS
Synonyms:RRS1
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 661661Arginine--tRNA ligase, cytoplasmicUniRule annotationPRO_0000035795Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP37880.

Interactioni

Subunit structurei

Interacts (via N-terminus) with AIMP1 (via N-terminus); this stimulates its catalytic activity. Interacts with EPRS (via N-terminus). Interacts with LARS2. Monomer; also part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro By similarity.

Structurei

3D structure databases

ProteinModelPortaliP37880.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7373Could be involved in the assembly of the multisynthetase complexUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi202 – 21312"HIGH" regionUniRule annotationAdd
BLAST

Domaini

The N-terminus (AA 1-73) has two regions predicted to be alpha-helical that might be involved in the multisynthetase complex assembly.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG029238.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform Complexed (identifier: P37880-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDGLVAQCSA RLLQQEKEIK SLTAEIDRLK NCGHLEDSPS LDQLREENLK    50
LKYRLNILQR SLQAEKRRRP TKNMININSR LQDVFGCAIK AAYPDLDNPP 100
LVVTPSQQPK FGDYQCNSAM GISQMLKAKE QKVNPRGIAE NITKHLPNNE 150
YIDRVEIAGP GFINVHLRKD FVSEQLTNLL VNGIQLPALG ENKKVIVDFS 200
SPNIAKEMHV GHLRSTIIGE SMSRLFEFAG YDVLRLNHVG DWGTQFGMLI 250
AHLQDQFPDY LTVSPPIGDL QAFYKESKKR FDTEEEFKKR AYQCVVSLQS 300
KDPDFIKAWN LICDVSRAEF NKIYDALDIT LIERGESFYQ DRMKDIVKEF 350
EDKGYVQVDD GRKIVFVPGC SIPLTIVKSD GGFTYDTSDL AAIKQRLFEE 400
KANKIIYVVD NGQAVHFQTI FAAAQMIGWY DPKVTQVTHV GFGVVLGEDK 450
KKFKTRSGET VRLVDLLGEG LKRSMDKLKE KERDKVLTEE ELTAAQTSIA 500
YGCIKYADLS HNRLNDYIFS FDKMLDDRGN TAAYLLYAFT RIRSIARLAN 550
VDEEMLQKAA RETKIVLDHE KEWKLGRCIL RFPEILQKML DDLFLHTLCD 600
YIYELATTFT EFYDSCYCVE KDRQTGKVLK VNMWRMLLCE AVAAVMAKGF 650
DILGIKPVQR M 661
Length:661
Mass (Da):75,602
Last modified:October 1, 1994 - v1
Checksum:i12EB1C85655EB8F4
GO
Isoform Monomeric (identifier: P37880-2) [UniParc]FASTAAdd to Basket

Also known as: Free

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.

Note: The alternative initiation site Met-74 is uncertain.

Show »
Length:588
Mass (Da):67,088
Checksum:i623A7303E0156F90
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7373Missing in isoform Monomeric. VSP_018904Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63415 mRNA. Translation: CAA45012.1.
RefSeqiXP_007632694.1. XM_007634504.1. [P37880-1]
XP_007632695.1. XM_007634505.1. [P37880-2]
XP_007632696.1. XM_007634506.1. [P37880-2]

Genome annotation databases

GeneIDi100767723.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63415 mRNA. Translation: CAA45012.1 .
RefSeqi XP_007632694.1. XM_007634504.1. [P37880-1 ]
XP_007632695.1. XM_007634505.1. [P37880-2 ]
XP_007632696.1. XM_007634506.1. [P37880-2 ]

3D structure databases

ProteinModelPortali P37880.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P37880.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100767723.

Phylogenomic databases

HOVERGENi HBG029238.

Enzyme and pathway databases

SABIO-RK P37880.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_00123. Arg_tRNA_synth.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view ]
PANTHERi PTHR11956. PTHR11956. 1 hit.
Pfami PF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view ]
PRINTSi PR01038. TRNASYNTHARG.
SMARTi SM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsi TIGR00456. argS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and analysis of a cDNA encoding mammalian arginyl-tRNA synthetase, a component of the multisynthetase complex with a hydrophobic N-terminal extension."
    Lazard M., Mirande M.
    Gene 132:237-245(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiSYRC_CRIGR
AccessioniPrimary (citable) accession number: P37880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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