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P37871

- RPOC_BACSU

UniProt

P37871 - RPOC_BACSU

Protein

DNA-directed RNA polymerase subunit beta'

Gene

rpoC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 4 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-HAMAP
    2. DNA-directed RNA polymerase activity Source: UniProtKB-HAMAP
    3. protein binding Source: IntAct

    GO - Biological processi

    1. response to antibiotic Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Antibiotic resistance, Transcription

    Enzyme and pathway databases

    BioCyciBSUB:BSU01080-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase subunit beta'UniRule annotation (EC:2.7.7.6UniRule annotation)
    Short name:
    RNAP subunit beta'UniRule annotation
    Alternative name(s):
    RNA polymerase subunit beta'UniRule annotation
    Transcriptase subunit beta'UniRule annotation
    Gene namesi
    Name:rpoCUniRule annotation
    Synonyms:lpm, std
    Ordered Locus Names:BSU01080
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU01080. [Micado]

    Subcellular locationi

    GO - Cellular componenti

    1. DNA-directed RNA polymerase complex Source: RefGenome

    Keywords - Cellular componenti

    DNA-directed RNA polymerase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11991199DNA-directed RNA polymerase subunit beta'PRO_0000067707Add
    BLAST

    Proteomic databases

    PaxDbiP37871.
    PRIDEiP37871.

    Interactioni

    Subunit structurei

    The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    spxAO316022EBI-5244361,EBI-5248631

    Protein-protein interaction databases

    DIPiDIP-49022N.
    IntActiP37871. 5 interactions.
    MINTiMINT-8364832.
    STRINGi224308.BSU01080.

    Structurei

    3D structure databases

    ProteinModelPortaliP37871.
    SMRiP37871. Positions 5-1197.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNA polymerase beta' chain family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0086.
    HOGENOMiHOG000218387.
    KOiK03046.
    OrthoDBiEOG6M9DS6.
    PhylomeDBiP37871.

    Family and domain databases

    HAMAPiMF_01322. RNApol_bact_RpoC.
    MF_01323. RNApol_bact_RpoC1.
    InterProiIPR012754. DNA-dir_RpoC_beta_prime.
    IPR000722. RNA_pol_asu.
    IPR006592. RNA_pol_N.
    IPR007080. RNA_pol_Rpb1_1.
    IPR007066. RNA_pol_Rpb1_3.
    IPR007083. RNA_pol_Rpb1_4.
    IPR007081. RNA_pol_Rpb1_5.
    [Graphical view]
    PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
    PF00623. RNA_pol_Rpb1_2. 1 hit.
    PF04983. RNA_pol_Rpb1_3. 1 hit.
    PF05000. RNA_pol_Rpb1_4. 1 hit.
    PF04998. RNA_pol_Rpb1_5. 1 hit.
    [Graphical view]
    SMARTiSM00663. RPOLA_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02386. rpoC_TIGR. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P37871-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLDVNNFEYM NIGLASPDKI RSWSFGEVKK PETINYRTLK PEKDGLFCER     50
    IFGPTKDWEC HCGKYKRVRY KGVVCDRCGV EVTRAKVRRE RMGHIELAAP 100
    VSHIWYFKGI PSRMGLVLDM SPRALEEVIY FASYVVTDPA NTPLEKKQLL 150
    SEKEYRAYLD KYGNKFQASM GAEAIHKLLQ DIDLVKEVDM LKEELKTSQG 200
    QRRTRAIKRL EVLEAFRNSG NKPSWMILDV LPVIPPELRP MVQLDGGRFA 250
    TSDLNDLYRR VINRNNRLKR LLDLGAPSII VQNEKRMLQE AVDALIDNGR 300
    RGRPVTGPGN RPLKSLSHML KGKQGRFRQN LLGKRVDYSG RSVIVVGPHL 350
    KMYQCGLPKE MALELFKPFV MKELVEKGLA HNIKSAKRKI ERVQPEVWDV 400
    LESVIKEHPV LLNRAPTLHR LGIQAFEPTL VEGRAIRLHP LVCTAYNADF 450
    DGDQMAVHVP LSAEAQAEAR ILMLAAQNIL NPKDGKPVVT PSQDMVLGNY 500
    YLTLERAGAV GEGMVFKNTD EALLAYQNGY VHLHTRVAVA ANSLKNVTFT 550
    EEQRSKLLIT TVGKLVFNEI LPESFPYMNE PTKSNIEEKT PDRFFLEKGA 600
    DVKAVIAQQP INAPFKKGIL GKIIAEIFKR FHITETSKML DRMKNLGFKY 650
    STKAGITVGV SDIVVLDDKQ EILEEAQSKV DNVMKQFRRG LITEEERYER 700
    VISIWSAAKD VIQGKLMKSL DELNPIYMMS DSGARGNASN FTQLAGMRGL 750
    MANPAGRIIE LPIKSSFREG LTVLEYFIST HGARKGLADT ALKTADSGYL 800
    TRRLVDVAQD VIIRETDCGT DRGILAKPLK EGTETIERLE ERLIGRFARK 850
    QVKHPETGEV LVNENELIDE DKALEIVEAG IEEVWIRSAF TCNTPHGVCK 900
    RCYGRNLATG SDVEVGEAVG IIAAQSIGEP GTQLTMRTFH TGGVAGDDIT 950
    QGLPRIQELF EARNPKGQAT ITEIDGTVVE INEVRDKQQE IVVQGAVETR 1000
    SYTAPYNSRL KVAEGDKITR GQVLTEGSID PKELLKVTDL TTVQEYLLHE 1050
    VQKVYRMQGV EIGDKHVEVM VRQMLRKVRV IDAGDTDVLP GTLLDIHQFT 1100
    EANKKVLLEG NRPATGRPVL LGITKASLET DSFLSAASFQ ETTRVLTDAA 1150
    IKGKRDELLG LKENVIIGKL VPAGTGMMKY RKVKPVSNVQ PTDDMVPVE 1199
    Length:1,199
    Mass (Da):134,252
    Last modified:June 16, 2009 - v4
    Checksum:i33263510698FC12B
    GO

    Sequence cautioni

    The sequence AAB59112.1 differs from that shown. Reason: Frameshift at positions 888 and 994.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti781 – 7811H → P in BAA10999. (PubMed:8969501)Curated
    Sequence conflicti1026 – 10261E → G in BAA10999. (PubMed:8969501)Curated
    Sequence conflicti1173 – 11731A → G in BAA10999. (PubMed:8969501)Curated
    Sequence conflicti1176 – 11761G → A in BAA10999. (PubMed:8969501)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti796 – 7961D → G in streptolydigan resistant alleles stl6/stl445.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43593 Genomic DNA. Translation: AAB59112.1. Frameshift.
    AL009126 Genomic DNA. Translation: CAB11884.2.
    L24376 Genomic DNA. Translation: AAB00973.1.
    D64127 Genomic DNA. Translation: BAA10999.1.
    PIRiG69698.
    RefSeqiNP_387989.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB11884; CAB11884; BSU01080.
    GeneIDi935977.
    KEGGibsu:BSU01080.
    PATRICi18971725. VBIBacSub10457_0111.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43593 Genomic DNA. Translation: AAB59112.1 . Frameshift.
    AL009126 Genomic DNA. Translation: CAB11884.2 .
    L24376 Genomic DNA. Translation: AAB00973.1 .
    D64127 Genomic DNA. Translation: BAA10999.1 .
    PIRi G69698.
    RefSeqi NP_387989.2. NC_000964.3.

    3D structure databases

    ProteinModelPortali P37871.
    SMRi P37871. Positions 5-1197.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-49022N.
    IntActi P37871. 5 interactions.
    MINTi MINT-8364832.
    STRINGi 224308.BSU01080.

    Proteomic databases

    PaxDbi P37871.
    PRIDEi P37871.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB11884 ; CAB11884 ; BSU01080 .
    GeneIDi 935977.
    KEGGi bsu:BSU01080.
    PATRICi 18971725. VBIBacSub10457_0111.

    Organism-specific databases

    GenoListi BSU01080. [Micado ]

    Phylogenomic databases

    eggNOGi COG0086.
    HOGENOMi HOG000218387.
    KOi K03046.
    OrthoDBi EOG6M9DS6.
    PhylomeDBi P37871.

    Enzyme and pathway databases

    BioCyci BSUB:BSU01080-MONOMER.

    Family and domain databases

    HAMAPi MF_01322. RNApol_bact_RpoC.
    MF_01323. RNApol_bact_RpoC1.
    InterProi IPR012754. DNA-dir_RpoC_beta_prime.
    IPR000722. RNA_pol_asu.
    IPR006592. RNA_pol_N.
    IPR007080. RNA_pol_Rpb1_1.
    IPR007066. RNA_pol_Rpb1_3.
    IPR007083. RNA_pol_Rpb1_4.
    IPR007081. RNA_pol_Rpb1_5.
    [Graphical view ]
    Pfami PF04997. RNA_pol_Rpb1_1. 1 hit.
    PF00623. RNA_pol_Rpb1_2. 1 hit.
    PF04983. RNA_pol_Rpb1_3. 1 hit.
    PF05000. RNA_pol_Rpb1_4. 1 hit.
    PF04998. RNA_pol_Rpb1_5. 1 hit.
    [Graphical view ]
    SMARTi SM00663. RPOLA_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02386. rpoC_TIGR. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Streptolydigin resistance can be conferred by alterations to either the beta or beta' subunits of Bacillus subtilis RNA polymerase."
      Yang X., Price C.W.
      J. Biol. Chem. 270:23930-23933(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], STREPTOLYDIGAN RESISTANCE.
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 1026; 1173 AND 1176.
    4. "Genetic and transcriptional organization of the region encoding the beta subunit of Bacillus subtilis RNA polymerase."
      Boor K.J., Duncan M.L., Price C.W.
      J. Biol. Chem. 270:20329-20336(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-466.
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    5. "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome."
      Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H.
      Microbiology 142:3039-3046(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 453-1199.
      Strain: 168.

    Entry informationi

    Entry nameiRPOC_BACSU
    AccessioniPrimary (citable) accession number: P37871
    Secondary accession number(s): P70978
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 104 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3