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P37871 (RPOC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit beta'

Short name=RNAP subunit beta'
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'
Gene names
Name:rpoC
Synonyms:lpm, std
Ordered Locus Names:BSU01080
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length1199 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP-Rule MF_01322

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01322

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Sequence similarities

Belongs to the RNA polymerase beta' chain family.

Sequence caution

The sequence AAB59112.1 differs from that shown. Reason: Frameshift at positions 888 and 994.

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

spxAO316022EBI-5244361,EBI-5248631

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11991199DNA-directed RNA polymerase subunit beta' HAMAP-Rule MF_01322
PRO_0000067707

Natural variations

Natural variant7961D → G in streptolydigan resistant alleles stl6/stl445.

Experimental info

Sequence conflict7811H → P in BAA10999. Ref.5
Sequence conflict10261E → G in BAA10999. Ref.5
Sequence conflict11731A → G in BAA10999. Ref.5
Sequence conflict11761G → A in BAA10999. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P37871 [UniParc].

Last modified June 16, 2009. Version 4.
Checksum: 33263510698FC12B

FASTA1,199134,252
        10         20         30         40         50         60 
MLDVNNFEYM NIGLASPDKI RSWSFGEVKK PETINYRTLK PEKDGLFCER IFGPTKDWEC 

        70         80         90        100        110        120 
HCGKYKRVRY KGVVCDRCGV EVTRAKVRRE RMGHIELAAP VSHIWYFKGI PSRMGLVLDM 

       130        140        150        160        170        180 
SPRALEEVIY FASYVVTDPA NTPLEKKQLL SEKEYRAYLD KYGNKFQASM GAEAIHKLLQ 

       190        200        210        220        230        240 
DIDLVKEVDM LKEELKTSQG QRRTRAIKRL EVLEAFRNSG NKPSWMILDV LPVIPPELRP 

       250        260        270        280        290        300 
MVQLDGGRFA TSDLNDLYRR VINRNNRLKR LLDLGAPSII VQNEKRMLQE AVDALIDNGR 

       310        320        330        340        350        360 
RGRPVTGPGN RPLKSLSHML KGKQGRFRQN LLGKRVDYSG RSVIVVGPHL KMYQCGLPKE 

       370        380        390        400        410        420 
MALELFKPFV MKELVEKGLA HNIKSAKRKI ERVQPEVWDV LESVIKEHPV LLNRAPTLHR 

       430        440        450        460        470        480 
LGIQAFEPTL VEGRAIRLHP LVCTAYNADF DGDQMAVHVP LSAEAQAEAR ILMLAAQNIL 

       490        500        510        520        530        540 
NPKDGKPVVT PSQDMVLGNY YLTLERAGAV GEGMVFKNTD EALLAYQNGY VHLHTRVAVA 

       550        560        570        580        590        600 
ANSLKNVTFT EEQRSKLLIT TVGKLVFNEI LPESFPYMNE PTKSNIEEKT PDRFFLEKGA 

       610        620        630        640        650        660 
DVKAVIAQQP INAPFKKGIL GKIIAEIFKR FHITETSKML DRMKNLGFKY STKAGITVGV 

       670        680        690        700        710        720 
SDIVVLDDKQ EILEEAQSKV DNVMKQFRRG LITEEERYER VISIWSAAKD VIQGKLMKSL 

       730        740        750        760        770        780 
DELNPIYMMS DSGARGNASN FTQLAGMRGL MANPAGRIIE LPIKSSFREG LTVLEYFIST 

       790        800        810        820        830        840 
HGARKGLADT ALKTADSGYL TRRLVDVAQD VIIRETDCGT DRGILAKPLK EGTETIERLE 

       850        860        870        880        890        900 
ERLIGRFARK QVKHPETGEV LVNENELIDE DKALEIVEAG IEEVWIRSAF TCNTPHGVCK 

       910        920        930        940        950        960 
RCYGRNLATG SDVEVGEAVG IIAAQSIGEP GTQLTMRTFH TGGVAGDDIT QGLPRIQELF 

       970        980        990       1000       1010       1020 
EARNPKGQAT ITEIDGTVVE INEVRDKQQE IVVQGAVETR SYTAPYNSRL KVAEGDKITR 

      1030       1040       1050       1060       1070       1080 
GQVLTEGSID PKELLKVTDL TTVQEYLLHE VQKVYRMQGV EIGDKHVEVM VRQMLRKVRV 

      1090       1100       1110       1120       1130       1140 
IDAGDTDVLP GTLLDIHQFT EANKKVLLEG NRPATGRPVL LGITKASLET DSFLSAASFQ 

      1150       1160       1170       1180       1190 
ETTRVLTDAA IKGKRDELLG LKENVIIGKL VPAGTGMMKY RKVKPVSNVQ PTDDMVPVE 

« Hide

References

« Hide 'large scale' references
[1]"Streptolydigin resistance can be conferred by alterations to either the beta or beta' subunits of Bacillus subtilis RNA polymerase."
Yang X., Price C.W.
J. Biol. Chem. 270:23930-23933(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], STREPTOLYDIGAN RESISTANCE.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 1026; 1173 AND 1176.
[4]"Genetic and transcriptional organization of the region encoding the beta subunit of Bacillus subtilis RNA polymerase."
Boor K.J., Duncan M.L., Price C.W.
J. Biol. Chem. 270:20329-20336(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-466.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[5]"Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome."
Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H.
Microbiology 142:3039-3046(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 453-1199.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43593 Genomic DNA. Translation: AAB59112.1. Frameshift.
AL009126 Genomic DNA. Translation: CAB11884.2.
L24376 Genomic DNA. Translation: AAB00973.1.
D64127 Genomic DNA. Translation: BAA10999.1.
PIRG69698.
RefSeqNP_387989.2. NC_000964.3.

3D structure databases

ProteinModelPortalP37871.
SMRP37871. Positions 5-1197.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-49022N.
IntActP37871. 5 interactions.
MINTMINT-8364832.
STRING224308.BSU01080.

Proteomic databases

PaxDbP37871.
PRIDEP37871.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB11884; CAB11884; BSU01080.
GeneID935977.
KEGGbsu:BSU01080.
PATRIC18971725. VBIBacSub10457_0111.

Organism-specific databases

GenoListBSU01080. [Micado]

Phylogenomic databases

eggNOGCOG0086.
HOGENOMHOG000218387.
KOK03046.
OrthoDBEOG6M9DS6.
ProtClustDBPRK00566.

Enzyme and pathway databases

BioCycBSUB:BSU01080-MONOMER.

Family and domain databases

HAMAPMF_01322. RNApol_bact_RpoC.
MF_01323. RNApol_bact_RpoC1.
InterProIPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTSM00663. RPOLA_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR02386. rpoC_TIGR. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRPOC_BACSU
AccessionPrimary (citable) accession number: P37871
Secondary accession number(s): P70978
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: June 16, 2009
Last modified: February 19, 2014
This is version 100 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList