Reviewed, UniProtKB/Swiss-Prot P37838 (NOP4_YEAST)

Names and origin

Protein names

Recommended name:
    Nucleolar protein 4
Alternative name(s):
    Nucleolar protein NOP77

Gene names

Name:	NOP4
Synonyms:	NOP77
Ordered Locus Names:	YPL043W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	685 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for 60S ribosomal subunit synthesis. Probably involved in the processing of 27S rRNA to produce mature 25S rRNA.
Subunit structure	Interacts with NOP1.
Subcellular location	Nucleus › nucleolus.
Miscellaneous	Present with 4990 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 4 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Traceable author statement. Source: SGD
Cellular component	nucleolus Traceable author statement. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EBP2	P36049	1	EBI-12122,EBI-6289
OSH3	P38713	1	EBI-12122,EBI-12630

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 685	685	Nucleolar protein 4		PRO_0000081677
Regions
	Domain	26 – 103	78	RRM 1
	Domain	147 – 225	79	RRM 2
	Domain	290 – 383	94	RRM 3
	Domain	462 – 612	151	RRM 4
	Compositional bias	241 – 267	27	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	117	1	Phosphoserine Ref.7
	Modified residue	118	1	Phosphothreonine Ref.7
	Modified residue	173	1	Phosphothreonine Ref.7
	Modified residue	247	1	Phosphoserine Ref.6
	Modified residue	379	1	Phosphothreonine Ref.5
Natural variations
	Natural variant	308	1	P → A

Sequences

	Sequence		Length	Mass (Da)
	P37838-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 61261815EA3DEE5C	FASTA	685	77,825
	10 20 30 40 50 60 MEETIENVEV PSSNVSKQND DGLDMKTLFV RSIPQDVTDE QLADFFSNFA PIKHAVVVKD 70 80 90 100 110 120 TNKRSRGFGF VSFAVEDDTK EALAKARKTK FNGHILRVDI AKRRDRSKKT SEVVEKSTPE 130 140 150 160 170 180 SSEKITGQNN EDEDDADGED SMLKGKPKLI IRNMPWSCRD PVKLKKIFGR YGTVVEATIP 190 200 210 220 230 240 RKRDGKLCGF AFVTMKKISN CRIALENTKD LKIDGRKVAV DFAVQKNRWE DYKKAQPEMN 250 260 270 280 290 300 DKDDNESGNE DAEENHDDEE DENEEEDRQV DQASKNKESK RKAQNKREDF SVFVRNVPYD 310 320 330 340 350 360 ATEESLAPHF SKFGSVKYAL PVIDKSTGLA KGTAFVAFKD QYTYNECIKN APAAGSTSLL 370 380 390 400 410 420 IGDDVMPEYV YEGRVLSITP TLVREDAGRM AEKNAAKRKE ALGKAPGEKD RRNLYLLNEG 430 440 450 460 470 480 RVVEGSKMAD LLTNTDMEIR EKSYKLRVEQ LKKNPSLHLS MTRLAIRNLP RAMNDKALKA 490 500 510 520 530 540 LARKAVVEFA TEVKNKERHP LSKEEIIRST KEKYKFMGPD EIEAQKKKDK KSGVVKQAKV 550 560 570 580 590 600 IMEVKGSTAG RSRGYGFVEF RDHKNALMGL RWLNCHAVTS DEILEGLNDD EKKQVDNDLG 610 620 630 640 650 660 KGRRLCVEFA IENSNVVKRR REQLKQARTK RTRPDNEDTG DVGESENKKP KKEEATTPTN 670 680 PDDKKMGDDI KRIIGFKRKR KHAKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast NOP4 gene product is an essential nucleolar protein required for pre-rRNA processing and accumulation of 60S ribosomal subunits." Sun C., Woolford J.L. Jr. EMBO J. 13:3127-3135(1994) [PubMed: 8039505] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Synthetic lethality with fibrillarin identifies NOP77p, a nucleolar protein required for pre-rRNA processing and modification." Berges T., Petfalski E., Tollervey D., Hurt E.C. EMBO J. 13:3136-3148(1994) [PubMed: 8039506] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-379, MASS SPECTROMETRY.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117; THR-118 AND THR-173, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U02598 Genomic DNA. Translation: AAA20590.1. X76245 Genomic DNA. Translation: CAA53824.1. U44030 Genomic DNA. Translation: AAB68177.1.
PIR	S46365.
RefSeq	NP_015282.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3891N.
IntAct	P37838. 63 interactions.
STRING	P37838.
Proteomic databases
PeptideAtlas	P37838.
PRIDE	P37838.
Genome annotation databases
Ensembl	YPL043W; YPL043W; YPL043W; Saccharomyces cerevisiae. [Genome view]
GeneID	856063.
KEGG	sce:YPL043W.
NMPDR	fig|4932.3.peg.6415.
Organism-specific databases
CYGD	YPL043w.
SGD	S000005964. NOP4.
Phylogenomic databases
HOGENOM	P37838.
OMA	QAKVIME
OrthoDB	EOG9V9W71
Gene expression databases
ArrayExpress	P37838.
Genevestigator	P37838.
GermOnline	YPL043W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR000504. RRM_RNP1. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 3 hits.
Pfam	PF00076. RRM_1. 3 hits. [Graphical view]
SMART	SM00360. RRM. 4 hits. [Graphical view]
PROSITE	PS50102. RRM. 4 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981044.

Entry information

Entry name

NOP4_YEAST

Accession

Primary (citable) accession number: P37838

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 24, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)