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Protein

Nucleolar protein 4

Gene

NOP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for 60S ribosomal subunit synthesis. Probably involved in the processing of 27S rRNA to produce mature 25S rRNA.

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • large ribosomal subunit rRNA binding Source: GO_Central
  • nucleotide binding Source: InterPro
  • RNA binding Source: SGD

GO - Biological processi

  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33957-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 4
Alternative name(s):
Nucleolar protein NOP77
Gene namesi
Name:NOP4
Synonyms:NOP77
Ordered Locus Names:YPL043W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL043W.
SGDiS000005964. NOP4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 685685Nucleolar protein 4PRO_0000081677Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei247 – 2471PhosphoserineCombined sources
Modified residuei379 – 3791PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP37838.

PTM databases

iPTMnetiP37838.

Interactioni

Subunit structurei

Interacts with NOP1.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-12122,EBI-12122
CIC1P387796EBI-12122,EBI-24538
DBP10Q123893EBI-12122,EBI-5644
DRS1P328925EBI-12122,EBI-6170
EBP2P360495EBI-12122,EBI-6289
HAS1Q035325EBI-12122,EBI-8170
LOC1P435864EBI-12122,EBI-22906
MAK21Q121768EBI-12122,EBI-10944
MAK5P381125EBI-12122,EBI-10394
NOC2P397445EBI-12122,EBI-29259
NOG1Q028924EBI-12122,EBI-12105
NOP12Q082085EBI-12122,EBI-35895
NUG1P400104EBI-12122,EBI-22449
PRP43P531313EBI-12122,EBI-505
RRP12Q127545EBI-12122,EBI-30678
RRP14P360803EBI-12122,EBI-26762

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi36136. 108 interactions.
DIPiDIP-3891N.
IntActiP37838. 69 interactions.
MINTiMINT-559282.

Structurei

3D structure databases

ProteinModelPortaliP37838.
SMRiP37838. Positions 38-103, 148-223, 531-580.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 10378RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini147 – 22579RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini290 – 38394RRM 3PROSITE-ProRule annotationAdd
BLAST
Domaini462 – 612151RRM 4PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi241 – 26727Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074976.
HOGENOMiHOG000187841.
InParanoidiP37838.
KOiK14573.
OMAiPVKHATV.
OrthoDBiEOG706125.

Family and domain databases

Gene3Di3.30.70.330. 5 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 5 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEETIENVEV PSSNVSKQND DGLDMKTLFV RSIPQDVTDE QLADFFSNFA
60 70 80 90 100
PIKHAVVVKD TNKRSRGFGF VSFAVEDDTK EALAKARKTK FNGHILRVDI
110 120 130 140 150
AKRRDRSKKT SEVVEKSTPE SSEKITGQNN EDEDDADGED SMLKGKPKLI
160 170 180 190 200
IRNMPWSCRD PVKLKKIFGR YGTVVEATIP RKRDGKLCGF AFVTMKKISN
210 220 230 240 250
CRIALENTKD LKIDGRKVAV DFAVQKNRWE DYKKAQPEMN DKDDNESGNE
260 270 280 290 300
DAEENHDDEE DENEEEDRQV DQASKNKESK RKAQNKREDF SVFVRNVPYD
310 320 330 340 350
ATEESLAPHF SKFGSVKYAL PVIDKSTGLA KGTAFVAFKD QYTYNECIKN
360 370 380 390 400
APAAGSTSLL IGDDVMPEYV YEGRVLSITP TLVREDAGRM AEKNAAKRKE
410 420 430 440 450
ALGKAPGEKD RRNLYLLNEG RVVEGSKMAD LLTNTDMEIR EKSYKLRVEQ
460 470 480 490 500
LKKNPSLHLS MTRLAIRNLP RAMNDKALKA LARKAVVEFA TEVKNKERHP
510 520 530 540 550
LSKEEIIRST KEKYKFMGPD EIEAQKKKDK KSGVVKQAKV IMEVKGSTAG
560 570 580 590 600
RSRGYGFVEF RDHKNALMGL RWLNCHAVTS DEILEGLNDD EKKQVDNDLG
610 620 630 640 650
KGRRLCVEFA IENSNVVKRR REQLKQARTK RTRPDNEDTG DVGESENKKP
660 670 680
KKEEATTPTN PDDKKMGDDI KRIIGFKRKR KHAKK
Length:685
Mass (Da):77,825
Last modified:October 1, 1994 - v1
Checksum:i61261815EA3DEE5C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti308 – 3081P → A.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02598 Genomic DNA. Translation: AAA20590.1.
X76245 Genomic DNA. Translation: CAA53824.1.
U44030 Genomic DNA. Translation: AAB68177.1.
BK006949 Genomic DNA. Translation: DAA11386.1.
PIRiS46365.
RefSeqiNP_015282.1. NM_001183857.1.

Genome annotation databases

EnsemblFungiiYPL043W; YPL043W; YPL043W.
GeneIDi856063.
KEGGisce:YPL043W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02598 Genomic DNA. Translation: AAA20590.1.
X76245 Genomic DNA. Translation: CAA53824.1.
U44030 Genomic DNA. Translation: AAB68177.1.
BK006949 Genomic DNA. Translation: DAA11386.1.
PIRiS46365.
RefSeqiNP_015282.1. NM_001183857.1.

3D structure databases

ProteinModelPortaliP37838.
SMRiP37838. Positions 38-103, 148-223, 531-580.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36136. 108 interactions.
DIPiDIP-3891N.
IntActiP37838. 69 interactions.
MINTiMINT-559282.

PTM databases

iPTMnetiP37838.

Proteomic databases

MaxQBiP37838.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL043W; YPL043W; YPL043W.
GeneIDi856063.
KEGGisce:YPL043W.

Organism-specific databases

EuPathDBiFungiDB:YPL043W.
SGDiS000005964. NOP4.

Phylogenomic databases

GeneTreeiENSGT00550000074976.
HOGENOMiHOG000187841.
InParanoidiP37838.
KOiK14573.
OMAiPVKHATV.
OrthoDBiEOG706125.

Enzyme and pathway databases

BioCyciYEAST:G3O-33957-MONOMER.

Miscellaneous databases

PROiP37838.

Family and domain databases

Gene3Di3.30.70.330. 5 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 5 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast NOP4 gene product is an essential nucleolar protein required for pre-rRNA processing and accumulation of 60S ribosomal subunits."
    Sun C., Woolford J.L. Jr.
    EMBO J. 13:3127-3135(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Synthetic lethality with fibrillarin identifies NOP77p, a nucleolar protein required for pre-rRNA processing and modification."
    Berges T., Petfalski E., Tollervey D., Hurt E.C.
    EMBO J. 13:3136-3148(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-379, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNOP4_YEAST
AccessioniPrimary (citable) accession number: P37838
Secondary accession number(s): D6W3X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 6, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4990 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.