Reviewed, UniProtKB/Swiss-Prot P37830 (G6PD_SOLTU)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform
      Short name=G6PD
    EC=1.1.1.49

Gene names

Name:

G6PDH

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to generate NADPH for reductive biosyntheses.
Catalytic activity	D-glucose 6-phosphate + NADP(+) = D-glucono-1,5-lactone 6-phosphate + NADPH.
Enzyme regulation	Regulated by metabolites.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Tissue specificity	Found in tubers, stolons, roots, and flower buds.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform		PRO_0000068101
Sites
	Active site	270	1	Proton acceptor By similarity
	Binding site	38	1	NADP By similarity
	Binding site	71	1	NADP By similarity
	Binding site	208	1	Substrate By similarity
	Binding site	212	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37830-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: BBD9E4891DA62671	FASTA	511	58,471
	10 20 30 40 50 60 MAASWCIEKR GSIRNDSFRD NDNIPETGCL SIIVLGASGD LAKKKTFPAL FNLYRQGFLQ 70 80 90 100 110 120 SNEVHIFGYA RTKISDDDLR SRIRGYLSQG KENEGEVSEF LQLIKYVSGS YDSAEGFTSL 130 140 150 160 170 180 DKAISEHEFS KNSTEGSSRR LFYFALPPSV YPSVCRMIKS YCMNKSDLGG WTRTVVEKPF 190 200 210 220 230 240 GKDLASSEQL SSQIGELFDE PQIYRIDHYL GKELVQNLLV LRFANRFFLP LWNRDNIDNI 250 260 270 280 290 300 QIVFREDFGT EGRGGYFDEY GIIRDIIQNH LLQVLCLVAM EKPVSQKPEH IRDEKVKVLQ 310 320 330 340 350 360 SMLPIEDEEV VLGQYEGYKD DPTVPNNSNT PTFATMVLRI HNERWEGVPF IMKAGKALNS 370 380 390 400 410 420 RKAEIRVQFK DVPGDIFRCQ KQGRNEFVIR LQPSEAMYMK LTVKKPGLEM STVQSELDLS 430 440 450 460 470 480 YGQRYQGVVI PEAYERLILD TIRGDQQHFV RRDELKAAWE IFTPLLHRID NGEVKPIPYK 490 500 510 PGSRGPAEAD ELLQNAGYVQ THGYIWIPPT L

References

[1]	"Purification, characterization, and cDNA sequence of glucose-6-phosphate dehydrogenase from potato (Solanum tuberosum L.)." Graeve K., von Schaewen A., Scheibe R. Plant J. 5:353-361(1994) [PubMed: 8180621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Desiree. Tissue: Green leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X74421 mRNA. Translation: CAA52442.1.
PIR	S60287.
3D structure databases
HSSP	HSSP built from PDB template 1QKI based on UniProtKB P11413.
ModBase	Search...
Family and domain databases
InterPro	IPR001282. Glc-6-P_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
ProDom	PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PD_SOLTU

Accession

Primary (citable) accession number: P37830

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 25, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)