P37800 (L_TOSV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Toscana virus (Tos)

Taxonomic identifier

11590 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Phlebovirus › Sandfly fever Naples virus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Phlebotomus perniciosus [TaxID: 13204]

Protein attributes

Sequence length	2095 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the phlebovirus RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleoside binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2095	2095	RNA-directed RNA polymerase L		PRO_0000222025
Regions
	Domain	976 – 1167	192	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P37800 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 22FF4DAD745583B7	FASTA	2,095	238,887
	10 20 30 40 50 60 MERILKKQPA PVRALTIHPL RRYESSIYDT PIPAYVIKHS SDGVTIDIAT SELADGQSGS 70 80 90 100 110 120 TIQPFESVPA QNLTLFKHDF TFGHLADTTD KKFVEVFGVL ENRADDSDFQ SPDMIIETET 130 140 150 160 170 180 GHVYVVEFTT TMGDANSADL AARNKIAKYE IACLNRSAIK PISLYIIAVH FNGVISNLDL 190 200 210 220 230 240 SDEEVNEIVF RFRLARDIFE ELREINPALF DSDETISRLE REVNSVMSAI QIDWDTTEKK 250 260 270 280 290 300 FPSFRRELFE NFRSKEVDDE YISKIIKRCT DEALRGIERD SLYTEDITNK ERFELNSKRA 310 320 330 340 350 360 ASDIKNKMAE MMSYEFLRDT EDHKSTVQFP PWVTRTGPAG KDLEPLKSVS VEGSHPMCKI 370 380 390 400 410 420 WNKVCTNASI EKIERMHDDP VLELEYAMSG STERSVERNK YHRTVLTLSP EEREYAAVLG 430 440 450 460 470 480 VCGKRNANLG AVKEARVRSK KGFSIGHNTE RVEEFLSDSC VEDLIPTEGL YNPLSEDKSL 490 500 510 520 530 540 RLLAMGLHQP TLIHMDDETP ETLDCHLKFL SSPIGSWLQM VSIVGAELSA SVKQHVKPNQ 550 560 570 580 590 600 FIVKRLLDSA IFLLIKPTTS KGHIFVSLAV NKKFLHGELS KSSVFKQSID AGDLLVTDFV 610 620 630 640 650 660 SFKLSKITNL CKALCVLEAA SCFWAETYGF EPWKFVDQAS AVKFLDAWFM IKLSLLTMLE 670 680 690 700 710 720 DKATTEELQT MQRYVIMEGF VSLPEIPKPH KMLSKIPKVL RSELQVFLTH RLFSTMQRIS 730 740 750 760 770 780 ATPFQLHKVG GNIRWKGLFN PYSGNSIDEL QTLISCCYNG YFKNKEEDTE PSALSAMYKK 790 800 810 820 830 840 IIELEHLRPP TDTYLGYEDP IDPKMHEFSR SYLKLLCNHA KTKLRKQYGR GVMNQIENSI 850 860 870 880 890 900 VREVQSITLE RLATLKATSN FDDSWYTFKD VKDKNYTRDK LLVKMTQFAH RGKTLAIEVF 910 920 930 940 950 960 EECMSRIEEK GCMEICLFKK QQHGGLREIY VMGADERIVQ SVIEAIARAI GRFFDSDTLC 970 980 990 1000 1010 1020 NPSNKIRIPE THGQRAKRRC GRSVWTCATS DDARKWNQGH YVTKFALMLC EFTPQEWWPL 1030 1040 1050 1060 1070 1080 IIRGCSMFTN KFMMMNLDFL RIIDSHKELQ IEDEFVSKLF KAYHGESVEP WISQGCTYLK 1090 1100 1110 1120 1130 1140 TSTGMMQGIL HFTSSLLHSL HQEFVKTTAI QLFTLKLGSD ASSKVVCDMM QGSDDSSMII 1150 1160 1170 1180 1190 1200 SFPSYNEKIK MRYKLVAAMC FRIKKSLGIY IGIYPSEKST PNTDFVMEYN SEFFFHSQHV 1210 1220 1230 1240 1250 1260 RPTIRWIAAS CSLPEVETLV ASKEEAANLL TAITEGGGSF SLAAMIQHCQ SSIHYMLMGL 1270 1280 1290 1300 1310 1320 GVSALFSEFS KAISKWLDPG LGFFLFDNPY SAGLSGFKYN LYRAIMNSSL KSIYSFFMKR 1330 1340 1350 1360 1370 1380 VKGGSQRTDG IISESCSVSP GGAIVMSSTL RWGSVEKFKR LRNRLNIPET WKEMINESPE 1390 1400 1410 1420 1430 1440 VLYRAPQTGT EIMLRIAEKV HSPGVVSSLS TGNAVCKVMA SSVYFLSACI FEDAGSQEYK 1450 1460 1470 1480 1490 1500 VVNNDKYSLM QKIIAFDQIG CNDEISQEDL LFLFPNLAEF EAFDSIIYDK GRFNVIPRAS 1510 1520 1530 1540 1550 1560 QREATQTRIV VFEHHSSARV APEKLVSDKW FGTRKSKIGS PGFRQEWDRL KAIVRWLRDT 1570 1580 1590 1600 1610 1620 PEETLDSSPF SNHIQIRNFF ARMEGRPRVI KVTGAPVKRD LGMSKIAMAI RDNFCKTGFL 1630 1640 1650 1660 1670 1680 QGLEDEVGHS RAMQVEKIKH YLFSVLMGPY SEEAKLEYVV KILKEEPQVI LNYNDKRSRA 1690 1700 1710 1720 1730 1740 NIISLLQRFI KSEIGIATLI EDMKAGVFGA FVKAQQFSQS SVNNKYYGRG IWKGVMDGYQ 1750 1760 1770 1780 1790 1800 VQIDIDGKEG MPSHLSGITI SNCSKTWILT QSLKAWCEDM QVYNNTDVSK ANPKANYWMY 1810 1820 1830 1840 1850 1860 GFKMYGSSYP YGCPIYLVRH DITNLGLLHD DDIDIKVRRN TINLFVRSKD KRPRDLHILS 1870 1880 1890 1900 1910 1920 YTPSDSDISS VSSKHIMEDE YFVYKGAFSV EPTRSWMLCQ PLPWSFVRPV LQVATGSRRS 1930 1940 1950 1960 1970 1980 PRQLDLERLR EIIRLCTESS IRNKVGTVYG QNRPEKFIEA EPIDMSEMFD MMLDEGMDDA 1990 2000 2010 2020 2030 2040 FEELADYLTV EEDPDYMDEV SFDDDSLNLF GPAHYKELQS LTVLAHPLMD DFVTRLVGKM 2050 2060 2070 2080 2090 GRPQIRRLLE KNVTTRDLRE LSELLFMALD RDPSQIREEL ILGDSPTEVP DDLLG

References

[1]

"Toscana virus genomic L segment: molecular cloning, coding strategy and amino acid sequence in comparison with other negative strand RNA viruses." Accardi L., Gro M.C., di Bonito P., Giorgi C. Virus Res. 27:119-131(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: ISS.PHL.3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68414 Genomic RNA. Translation: CAA48478.1.
PIR	S29529.
RefSeq	YP_089669.1. NC_006319.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3077272.
Family and domain databases
InterPro	IPR022531. DUF3770. IPR007099. RNA-dir_pol_NSvirus. IPR014385. RNA-dir_pol_phlebovirus. IPR007322. RNA_pol_bunyavir. [Graphical view]
Pfam	PF04196. Bunya_RdRp. 1 hit. PF12603. DUF3770. 1 hit. [Graphical view]
PIRSF	PIRSF000826. L_PhleboV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_TOSV

Accession

Primary (citable) accession number: P37800

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program