P37776 (GALF_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UTP--glucose-1-phosphate uridylyltransferase
EC=2.7.7.9

Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
UDP-glucose pyrophosphorylase
Short name=UDPGP
Uridine diphosphoglucose pyrophosphorylase

Gene names

Name:	galF
Ordered Locus Names:	SF2105, S2228

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Pathway	Carbohydrate metabolism; nucleotide-sugar metabolism. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Sequence similarities	Belongs to the UDPGP type 2 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of catalytic activity Inferred from electronic annotation. Source: GOC
Molecular_function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC enzyme regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	UTP--glucose-1-phosphate uridylyltransferase		PRO_0000201353
Experimental info
	Sequence conflict	31 – 32	2	IV → TA in CAA50766. Ref.1
	Sequence conflict	285	1	A → S in CAA50766. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37776 [UniParc]. Last modified January 10, 2003. Version 2. Checksum: D693305ECBD9DF8A	FASTA	297	32,841
	10 20 30 40 50 60 MTNLKAVIPV AGLGMHMLPA TKAIPKEMLP IVDKPMIQYI VDEIVAAGIK EILLVTHASK 70 80 90 100 110 120 NAVENHFDTS YELESLLEQR VKRQLLAEVQ SICPPGVIIM NVRQGEPLGL GHSILCARPA 130 140 150 160 170 180 IGDNPFVVVL PDVVIDDASA DPLRYNLAAM IARFNETGRS QVLAKRMPGD LSEYSVIQTK 190 200 210 220 230 240 EPLDREGKVS RIVEFIEKPD QPQTLDSDIM AVGRYVLSAD IWPELERTQP GAWGRIQLTD 250 260 270 280 290 AIAELAKKQS VDAMLMTGDS YDCGKKMGYM QAFVKYGLRN LKEGAKFRKG IEKLLSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the dTDP-rhamnose biosynthetic genes encoded in the rfb locus of Shigella flexneri." Macpherson D.F., Manning P.A., Morona R. Mol. Microbiol. 11:281-292(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PE577 / Serotype 2a.
[2]	"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.
[3]	"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.
[4]	"Nucleotide sequence of the rhamnose biosynthetic operon of Shigella flexneri 2a and role of lipopolysaccharide in virulence." Rajakumar K., Jost B.H., Sasakawa C., Okada N., Yoshikawa M., Adler B. J. Bacteriol. 176:2362-2373(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 169-297. Strain: YSH6200 / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71970 Genomic DNA. Translation: CAA50766.1. AE005674 Genomic DNA. Translation: AAN43644.2. AE014073 Genomic DNA. Translation: AAP17473.1. L14842 Genomic DNA. Translation: AAA53678.1.
PIR	S41533.
RefSeq	NP_707937.2. NC_004337.2. NP_837664.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P37776.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF2105.
Proteomic databases
PaxDb	P37776.
PRIDE	P37776.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN43644; AAN43644; SF2105. AAP17473; AAP17473; S2228.
GeneID	1025313. 1078506.
KEGG	sfl:SF2105. sfx:S2228.
PATRIC	18705963. VBIShiFle31049_2460.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1210.
HOGENOM	HOG000283477.
KO	K00963.
ProtClustDB	PRK10122.
Enzyme and pathway databases
UniPathway	UPA00030. UPA00215.
Family and domain databases
InterPro	IPR005774. GalF. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01105. galF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALF_SHIFL

Accession

Primary (citable) accession number: P37776
Secondary accession number(s): Q54161

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 10, 2003
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program