Reviewed, UniProtKB/Swiss-Prot P37776 (GALF_SHIFL)

Names and origin

Protein names

Recommended name:
    UTP--glucose-1-phosphate uridylyltransferase
    EC=2.7.7.9
Alternative name(s):
    UDP-glucose pyrophosphorylase
      Short name=UDPGP
    Alpha-D-glucosyl-1-phosphate uridylyltransferase
    Uridine diphosphoglucose pyrophosphorylase

Gene names

Name:	galF
Ordered Locus Names:	SF2105, S2228

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Pathway	Carbohydrate metabolism; nucleotide-sugar metabolism. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Sequence similarities	Belongs to the UDPGP type 2 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC enzyme regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	UTP--glucose-1-phosphate uridylyltransferase		PRO_0000201353
Experimental info
	Sequence conflict	31 – 32	2	IV → TA in CAA50766. Ref.1
	Sequence conflict	285	1	A → S in CAA50766. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37776-1 [UniParc]. Last modified January 10, 2003. Version 2. Checksum: D693305ECBD9DF8A	FASTA	297	32,841
	10 20 30 40 50 60 MTNLKAVIPV AGLGMHMLPA TKAIPKEMLP IVDKPMIQYI VDEIVAAGIK EILLVTHASK 70 80 90 100 110 120 NAVENHFDTS YELESLLEQR VKRQLLAEVQ SICPPGVIIM NVRQGEPLGL GHSILCARPA 130 140 150 160 170 180 IGDNPFVVVL PDVVIDDASA DPLRYNLAAM IARFNETGRS QVLAKRMPGD LSEYSVIQTK 190 200 210 220 230 240 EPLDREGKVS RIVEFIEKPD QPQTLDSDIM AVGRYVLSAD IWPELERTQP GAWGRIQLTD 250 260 270 280 290 AIAELAKKQS VDAMLMTGDS YDCGKKMGYM QAFVKYGLRN LKEGAKFRKG IEKLLSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the dTDP-rhamnose biosynthetic genes encoded in the rfb locus of Shigella flexneri." Macpherson D.F., Manning P.A., Morona R. Mol. Microbiol. 11:281-292(1994) [PubMed: 8170390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PE577 / Serotype 2a.
[2]	"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.
[3]	"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.
[4]	"Nucleotide sequence of the rhamnose biosynthetic operon of Shigella flexneri 2a and role of lipopolysaccharide in virulence." Rajakumar K., Jost B.H., Sasakawa C., Okada N., Yoshikawa M., Adler B. J. Bacteriol. 176:2362-2373(1994) [PubMed: 8157605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 169-297. Strain: YSH6200 / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71970 Genomic DNA. Translation: CAA50766.1. AE005674 Genomic DNA. Translation: AAN43644.1. Different initiation. AE014073 Genomic DNA. Translation: AAP17473.1. L14842 Genomic DNA. Translation: AAA53678.1.
PIR	S41533.
RefSeq	NP_707937.2. NP_837664.1.
3D structure databases
SMR	P37776. Positions 4-297.
ModBase	Search...
Genome annotation databases
GeneID	1025313. 1078506.
GenomeReviews	Gene locus SF2105 in contig AE005674_GR. Gene locus S2228 in contig AE014073_GR.
KEGG	sfl:SF2105. sfx:S2228.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG688195.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN43644.1-MONOMER.
BRENDA	2.7.7.9. 189495.
Family and domain databases
InterPro	IPR005774. GalF. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01105. galF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALF_SHIFL

Accession

Primary (citable) accession number: P37776
Secondary accession number(s): Q54161

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 10, 2003
Last modified:	February 9, 2010
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)