UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase

Contribute

Send feedback

Read comments (?) or add your own

P37773 (MPL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase
EC=6.3.2.-

Alternative name(s):
Murein peptide ligase

Gene names

Name:	mpl
Synonyms:	yjfG
Ordered Locus Names:	b4233, JW4192

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reutilizes the intact tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid.
Pathway	Cell wall biogenesis; peptidoglycan recycling.
Subcellular location	Secreted.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Secreted
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: EcoliWiki extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acid-amino acid ligase activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rarA	P0AAZ4	1	EBI-562735,EBI-560358

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 457

457

UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase

PRO_0000101708

Regions

Nucleotide binding

110 – 116

ATP Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P37773 [UniParc].

Last modified July 15, 1998. Version 3.
Checksum: 105383EA5B696844
Show »

FASTA

457

49,874

        10         20         30         40         50         60 
MRIHILGICG TFMGGLAMLA RQLGHEVTGS DANVYPPMST LLEKQGIELI QGYDASQLEP 

        70         80         90        100        110        120 
QPDLVIIGNA MTRGNPCVEA VLEKNIPYMS GPQWLHDFVL RDRWVLAVAG THGKTTTAGM 

       130        140        150        160        170        180 
ATWILEQCGY KPGFVIGGVP GNFEVSAHLG ESDFFVIEAD EYDCAFFDKR SKFVHYCPRT 

       190        200        210        220        230        240 
LILNNLEFDH ADIFDDLKAI QKQFHHLVRI VPGQGRIIWP ENDINLKQTM AMGCWSEQEL 

       250        260        270        280        290        300 
VGEQGHWQAK KLTTDASEWE VLLDGEKVGE VKWSLVGEHN MHNGLMAIAA ARHVGVAPAD 

       310        320        330        340        350        360 
AANALGSFIN ARRRLELRGE ANGVTVYDDF AHHPTAILAT LAALRGKVGG TARIIAVLEP 

       370        380        390        400        410        420 
RSNTMKMGIC KDDLAPSLGR ADEVFLLQPA HIPWQVAEVA EACVQPAHWS GDVDTLADMV 

       430        440        450 
VKTAQPGDHI LVMSNGGFGG IHQKLLDGLA KKAEAAQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sequence of the Escherichia coli fructose-1,6-bisphosphatase gene." Hamilton W.D.O., Harrison D.A., Dyer T.A. Nucleic Acids Res. 16:8707-8707(1988) [PubMed: 2843822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64.
[5]	"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Borodovsky M., Rudd K.E., Koonin E.V. Nucleic Acids Res. 22:4756-4767(1994) [PubMed: 7984428] [Abstract] Cited for: IDENTIFICATION.
[6]	"Identification of the mpl gene encoding UDP-N-acetylmuramate: L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase in Escherichia coli and its role in recycling of cell wall peptidoglycan." Mengin-Lecreulx D., van Heijenoort J., Park J.T. J. Bacteriol. 178:5347-5352(1996) [PubMed: 8808921] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14003 Genomic DNA. Translation: AAA97130.1. U00096 Genomic DNA. Translation: AAC77190.1. AP009048 Genomic DNA. Translation: BAE78233.1. X12545 mRNA. No translation available.
PIR	S56459.
RefSeq	NP_418654.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P37773.
SMR	P37773. Positions 1-453.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10246N.
IntAct	P37773. 5 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000973; EBESCP00000000973; EBESCG00000000801. EBESCT00000000974; EBESCP00000000974; EBESCG00000000801. EBESCT00000018246; EBESCP00000017537; EBESCG00000017301.
GeneID	948752.
GenomeReviews	Gene locus JW4192 in contig AP009048_GR. Gene locus b4233 in contig U00096_GR.
KEGG	ecj:JW4192. eco:b4233.
PATRIC	32124039. VBIEscCol129921_4364.
Organism-specific databases
EchoBASE	EB2335.
EcoGene	EG12440. mpl.
Phylogenomic databases
eggNOG	COG0773.
GeneTree	EBGT00050000009066.
HOGENOM	HBG476594.
OMA	PYTSGPQ.
PhylomeDB	P37773.
ProtClustDB	CLSK880878.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12440-MONOMER. MetaCyc:EG12440-MONOMER.
Gene expression databases
Genevestigator	P37773.
Family and domain databases
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005757. Murein_peptide_ligase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K02558.
PANTHER	PTHR23135:SF8. PTHR23135:SF8. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01081. Mpl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MPL_ECOLI

Accession

Primary (citable) accession number: P37773
Secondary accession number(s): P76804, Q2M673

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	July 15, 1998
Last modified:	January 25, 2012
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents