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P37773 (MPL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase

EC=6.3.2.-
Alternative name(s):
Murein peptide ligase
Gene names
Name:mpl
Synonyms:yjfG
Ordered Locus Names:b4233, JW4192
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reutilizes the intact tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid.

Pathway

Cell wall biogenesis; peptidoglycan recycling.

Subcellular location

Secreted.

Sequence similarities

Belongs to the MurCDEF family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

rarAP0AAZ41EBI-562735,EBI-560358

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 457457UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase
PRO_0000101708

Regions

Nucleotide binding110 – 1167ATP Potential

Sequences

Sequence LengthMass (Da)Tools
P37773 [UniParc].

Last modified July 15, 1998. Version 3.
Checksum: 105383EA5B696844

FASTA45749,874
        10         20         30         40         50         60 
MRIHILGICG TFMGGLAMLA RQLGHEVTGS DANVYPPMST LLEKQGIELI QGYDASQLEP 

        70         80         90        100        110        120 
QPDLVIIGNA MTRGNPCVEA VLEKNIPYMS GPQWLHDFVL RDRWVLAVAG THGKTTTAGM 

       130        140        150        160        170        180 
ATWILEQCGY KPGFVIGGVP GNFEVSAHLG ESDFFVIEAD EYDCAFFDKR SKFVHYCPRT 

       190        200        210        220        230        240 
LILNNLEFDH ADIFDDLKAI QKQFHHLVRI VPGQGRIIWP ENDINLKQTM AMGCWSEQEL 

       250        260        270        280        290        300 
VGEQGHWQAK KLTTDASEWE VLLDGEKVGE VKWSLVGEHN MHNGLMAIAA ARHVGVAPAD 

       310        320        330        340        350        360 
AANALGSFIN ARRRLELRGE ANGVTVYDDF AHHPTAILAT LAALRGKVGG TARIIAVLEP 

       370        380        390        400        410        420 
RSNTMKMGIC KDDLAPSLGR ADEVFLLQPA HIPWQVAEVA EACVQPAHWS GDVDTLADMV 

       430        440        450 
VKTAQPGDHI LVMSNGGFGG IHQKLLDGLA KKAEAAQ 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Sequence of the Escherichia coli fructose-1,6-bisphosphatase gene."
Hamilton W.D.O., Harrison D.A., Dyer T.A.
Nucleic Acids Res. 16:8707-8707(1988) [PubMed: 2843822] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64.
[5]"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
Borodovsky M., Rudd K.E., Koonin E.V.
Nucleic Acids Res. 22:4756-4767(1994) [PubMed: 7984428] [Abstract]
Cited for: IDENTIFICATION.
[6]"Identification of the mpl gene encoding UDP-N-acetylmuramate: L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase in Escherichia coli and its role in recycling of cell wall peptidoglycan."
Mengin-Lecreulx D., van Heijenoort J., Park J.T.
J. Bacteriol. 178:5347-5352(1996) [PubMed: 8808921] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U14003 Genomic DNA. Translation: AAA97130.1.
U00096 Genomic DNA. Translation: AAC77190.1.
AP009048 Genomic DNA. Translation: BAE78233.1.
X12545 mRNA. No translation available.
PIRS56459.
RefSeqNP_418654.1. NC_000913.2.

3D structure databases

ProteinModelPortalP37773.
SMRP37773. Positions 1-453.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10246N.
IntActP37773. 5 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000000973; EBESCP00000000973; EBESCG00000000801.
EBESCT00000000974; EBESCP00000000974; EBESCG00000000801.
EBESCT00000018246; EBESCP00000017537; EBESCG00000017301.
GeneID948752.
GenomeReviewsGene locus JW4192 in contig AP009048_GR.
Gene locus b4233 in contig U00096_GR.
KEGGecj:JW4192.
eco:b4233.
PATRIC32124039. VBIEscCol129921_4364.

Organism-specific databases

EchoBASEEB2335.
EcoGeneEG12440. mpl.

Phylogenomic databases

eggNOGCOG0773.
GeneTreeEBGT00050000009066.
HOGENOMHBG476594.
OMAPYTSGPQ.
PhylomeDBP37773.
ProtClustDBCLSK880878.

Enzyme and pathway databases

BioCycEcoCyc:EG12440-MONOMER.
MetaCyc:EG12440-MONOMER.

Gene expression databases

GenevestigatorP37773.

Family and domain databases

InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005757. Murein_peptide_ligase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK02558.
PANTHERPTHR23135:SF8. PTHR23135:SF8. 1 hit.
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01081. Mpl. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMPL_ECOLI
AccessionPrimary (citable) accession number: P37773
Secondary accession number(s): P76804, Q2M673
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 15, 1998
Last modified: January 25, 2012
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families