P37772 (YJFF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inner membrane ABC transporter permease protein YjfF

Gene names

Name:	yjfF
Ordered Locus Names:	b4231, JW5754

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. AraH/RbsC subfamily.
Sequence caution	The sequence AAA97128.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	galactose uniporter activity Inferred from direct assay PubMed 19744923. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Inner membrane ABC transporter permease protein YjfF		PRO_0000060260
Regions
	Topological domain	1 – 5	5	Periplasmic Potential
	Transmembrane	6 – 26	21	Helical; Potential
	Topological domain	27 – 42	16	Cytoplasmic Potential
	Transmembrane	43 – 63	21	Helical; Potential
	Topological domain	64	1	Periplasmic Potential
	Transmembrane	65 – 85	21	Helical; Potential
	Topological domain	86 – 88	3	Cytoplasmic Potential
	Transmembrane	89 – 109	21	Helical; Potential
	Topological domain	110 – 113	4	Periplasmic Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Topological domain	135 – 159	25	Cytoplasmic Potential
	Transmembrane	160 – 180	21	Helical; Potential
	Topological domain	181 – 222	42	Periplasmic Potential
	Transmembrane	223 – 243	21	Helical; Potential
	Topological domain	244 – 251	8	Cytoplasmic Potential
	Transmembrane	252 – 272	21	Helical; Potential
	Topological domain	273 – 294	22	Periplasmic Potential
	Transmembrane	295 – 315	21	Helical; Potential
	Topological domain	316 – 331	16	Cytoplasmic Potential
Experimental info
	Sequence conflict	257	1	L → V in X12545. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P37772 [UniParc]. Last modified August 14, 2001. Version 3. Checksum: A30435AA393D6C9F	FASTA	331	34,978
	10 20 30 40 50 60 MIKRNLPLMI TIGVFVLGYL YCLTQFPGFA STRVICNILT DNAFLGIIAV GMTFVILSGG 70 80 90 100 110 120 IDLSVGSVIA FTGVFLAKVI GDFGLSPLLA FPLVLVMGCA FGAFMGLLID ALKIPAFIIT 130 140 150 160 170 180 LAGMFFLRGV SYLVSEESIP INHPIYDTLS SLAWKIPGGG RLSAMGLLML AVVVIGIFLA 190 200 210 220 230 240 HRTRFGNQVY AIGGNATSAN LMGISTRSTT IRIYMLSTGL ATLAGIVFSI YTQAGYALAG 250 260 270 280 290 300 VGVELDAIAS VVIGGTLLSG GVGTVLGTLF GVAIQGLIQT YINFDGTLSS WWTKIAIGIL 310 320 330 LFIFIALQRG LTVLWENRQS SPVTRVNIAQ Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sequence of the Escherichia coli fructose-1,6-bisphosphatase gene." Hamilton W.D.O., Harrison D.A., Dyer T.A. Nucleic Acids Res. 16:8707-8707(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 257-331.
[5]	"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Borodovsky M., Rudd K.E., Koonin E.V. Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14003 Genomic DNA. Translation: AAA97128.1. Different initiation. U00096 Genomic DNA. Translation: AAC77188.2. AP009048 Genomic DNA. Translation: BAE78231.1. X12545 mRNA. No translation available.
PIR	S56457.
RefSeq	NP_418652.2. NC_000913.2. YP_492372.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P37772.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b4231.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77188; AAC77188; b4231. BAE78231; BAE78231; BAE78231.
GeneID	12930321. 948754.
KEGG	ecj:Y75_p4116. eco:b4231.
PATRIC	32124035. VBIEscCol129921_4362.
Organism-specific databases
EchoBASE	EB2334.
EcoGene	EG12439. yjfF.
Phylogenomic databases
eggNOG	COG1172.
HOGENOM	HOG000212232.
KO	K02057.
OMA	AGMFFVR.
ProtClustDB	PRK11618.
Enzyme and pathway databases
BioCyc	EcoCyc:YJFF-MONOMER. ECOL316407:JW5754-MONOMER.
Gene expression databases
Genevestigator	P37772.
Family and domain databases
InterPro	IPR001851. ABC_transp_permease. [Graphical view]
Pfam	PF02653. BPD_transp_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YJFF_ECOLI

Accession

Primary (citable) accession number: P37772
Secondary accession number(s): Q2M675

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	August 14, 2001
Last modified:	May 1, 2013
This is version 104 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program