Reviewed, UniProtKB/Swiss-Prot P37769 (KDUD_ECOLI)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
    EC=1.1.1.127
Alternative name(s):
    2-keto-3-deoxygluconate oxidoreductase
      Short name=KDG oxidoreductase
    2-keto-3-deoxygluconate 5-dehydrogenase

Gene names

Name:	kduD
Synonyms:	ygeC, yqeD
Ordered Locus Names:	b2842, JW2810

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of 2,5-diketo-3-deoxygluconate (DKII or 4,6-dihydroxy-2,5-dioxohexanoate) into 2-keto-3-deoxygluconate (KDG or 2-dehydro-3-deoxygluconate) with a concomitant oxidation of NADH By similarity.
Catalytic activity	2-dehydro-3-deoxy-D-gluconate + NAD+ = (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase activity Inferred from electronic annotation. Source: EC 2-deoxy-D-gluconate 3-dehydrogenase activity Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase		PRO_0000054715
Regions
	Nucleotide binding	14 – 38	25	NAD By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	145	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37769-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: C751D4C2CEC6FAA0	FASTA	253	27,070
	10 20 30 40 50 60 MILSAFSLEG KVAVVTGCDT GLGQGMALGL AQAGCDIVGI NIVEPTETIE QVTALGRRFL 70 80 90 100 110 120 SLTADLRKID GIPALLDRAV AEFGHIDILV NNAGLIRRED ALEFSEKDWD DVMNLNIKSV 130 140 150 160 170 180 FFMSQAAAKH FIAQGNGGKI INIASMLSFQ GGIRVPSYTA SKSGVMGVTR LMANEWAKHN 190 200 210 220 230 240 INVNAIAPGY MATNNTQQLR ADEQRSAEIL DRIPAGRWGL PSDLMGPIVF LASSASDYVN 250 GYTIAVDGGW LAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The cloning, DNA sequence, and overexpression of the gene araE coding for arabinose-proton symport in Escherichia coli K12." Maiden M.C.J., Jones-Mortimer M.C., Henderson P.J.F. J. Biol. Chem. 263:8003-8010(1988) [PubMed: 2836407] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 216-253. Strain: K12 / JM2433.
[4]	"Analysis of an Erwinia chrysanthemi gene cluster involved in pectin degradation." Condemine G., Robert-Baudouy J. Mol. Microbiol. 5:2191-2202(1991) [PubMed: 1766386] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U29581 Genomic DNA. Translation: AAB40489.1. U00096 Genomic DNA. Translation: AAC75881.1. AP009048 Genomic DNA. Translation: BAE76911.1. J03732 Genomic DNA. No translation available.
PIR	C65067.
RefSeq	AP_003405.1. NP_417319.1.
3D structure databases
HSSP	HSSP built from PDB template 1FK8 based on UniProtKB Q9ZFY9.
ModBase	Search...
Protein-protein interaction databases
STRING	P37769.
Genome annotation databases
GeneID	947323.
GenomeReviews	Gene locus JW2810 in contig AP009048_GR. Gene locus b2842 in contig U00096_GR.
KEGG	ecj:JW2810. eco:b2842.
Organism-specific databases
EchoBASE	EB2264.
EcoGene	EG12361. kduD.
CMR	Search...
Phylogenomic databases
HOGENOM	P37769.
OMA	TIERVTA.
Enzyme and pathway databases
BioCyc	EcoCyc:KDUD-MON.
Gene expression databases
Genevestigator	P37769.
Family and domain databases
InterPro	IPR011286. 2-deoxy-D-gluc_3_DH. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01832. kduD. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDUD_ECOLI

Accession

Primary (citable) accession number: P37769
Secondary accession number(s): Q2M9Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)