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P37766 (YDIF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetate CoA-transferase YdiF

EC=2.8.3.8
Alternative name(s):
Short-chain acyl-CoA:acetate CoA-transferase
Gene names
Name:ydiF
Ordered Locus Names:b1694, JW1684
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length531 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

CoA transferase having broad substrate specificity for short-chain acyl-CoA thioesters with the activity decreasing when the length of the carboxylic acid chain exceeds four carbons. May play a role in short-chain fatty acid metabolism in E.coli By similarity.

Catalytic activity

Acyl-CoA + acetate = a fatty acid anion + acetyl-CoA.

Subunit structure

Homotetramer; dimer of dimers By similarity.

Miscellaneous

Formation of the covalent enzyme-CoA thioester intermediate proceeds via an unstable anhydride species formed between the carboxylate group of the catalytic glutamate of the enzyme and the carbonyl carbon of the thioester linkage of the substrate By similarity.

Sequence similarities

Belongs to the 3-oxoacid CoA-transferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 531531Acetate CoA-transferase YdiF
PRO_0000168990

Sites

Active site33315-glutamyl coenzyme A thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P37766 [UniParc].

Last modified December 1, 2000. Version 3.
Checksum: E07A0D445015030D

FASTA53157,562
        10         20         30         40         50         60 
MKPVKPPRIN GRVPVLSAQE AVNYIPDEAT LCVLGAGGGI LEATTLITAL ADKYKQTQTP 

        70         80         90        100        110        120 
RNLSIISPTG LGDRADRGIS PLAQEGLVKW ALCGHWGQSP RISELAEQNK IIAYNYPQGV 

       130        140        150        160        170        180 
LTQTLRAAAA HQPGIISDIG IGTFVDPRQQ GGKLNEVTKE DLIKLVEFDN KEYLYYKAIA 

       190        200        210        220        230        240 
PDIAFIRATT CDSEGYATFE DEVMYLDALV IAQAVHNNGG IVMMQVQKMV KKATLHPKSV 

       250        260        270        280        290        300 
RIPGYLVDIV VVDPDQTQLY GGAPVNRFIS GDFTLDDSTK LSLPLNQRKL VARRALFEMR 

       310        320        330        340        350        360 
KGAVGNVGVG IADGIGLVAR EEGCADDFIL TVETGPIGGI TSQGIAFGAN VNTRAILDMT 

       370        380        390        400        410        420 
SQFDFYHGGG LDVCYLSFAE VDQHGNVGVH KFNGKIMGTG GFIDISATSK KIIFCGTLTA 

       430        440        450        460        470        480 
GSLKTEITDG KLNIVQEGRV KKFIRELPEI TFSGKIALER GLDVRYITER AVFTLKEDGL 

       490        500        510        520        530 
HLIEIAPGVD LQKDILDKMD FTPVISPELK LMDERLFIDA AMGFVLPEAA H 

« Hide

References

« Hide 'large scale' references
[1]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase."
Duncan K., Chaudhuri S., Campbell M.S., Coggins J.R.
Biochem. J. 238:475-483(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64.
[5]"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
Borodovsky M., Rudd K.E., Koonin E.V.
Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74764.1.
AP009048 Genomic DNA. Translation: BAA15447.2.
X04306 Genomic DNA. No translation available.
PIRF64927.
RefSeqNP_416209.1. NC_000913.3.
YP_489956.1. NC_007779.1.

3D structure databases

ProteinModelPortalP37766.
SMRP37766. Positions 4-529.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP37766. 2 interactions.
STRING511145.b1694.

Proteomic databases

PaxDbP37766.
PRIDEP37766.

Protocols and materials databases

DNASU946211.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74764; AAC74764; b1694.
BAA15447; BAA15447; BAA15447.
GeneID12931296.
946211.
KEGGecj:Y75_p1669.
eco:b1694.
PATRIC32118694. VBIEscCol129921_1765.

Organism-specific databases

EchoBASEEB2328.
EcoGeneEG12432. ydiF.

Phylogenomic databases

eggNOGCOG4670.
HOGENOMHOG000058376.
OMAASDLKVM.
OrthoDBEOG6Z3KMD.
PhylomeDBP37766.

Enzyme and pathway databases

BioCycEcoCyc:EG12432-MONOMER.
ECOL316407:JW1684-MONOMER.
RETL1328306-WGS:GSTH-1755-MONOMER.

Gene expression databases

GenevestigatorP37766.

Family and domain databases

InterProIPR014388. 3-oxoacid_CoA-transferase.
IPR004165. CoA_trans_fam_I.
[Graphical view]
PANTHERPTHR13707. PTHR13707. 1 hit.
PfamPF01144. CoA_trans. 1 hit.
[Graphical view]
PIRSFPIRSF000858. SCOT-t. 1 hit.
SMARTSM00882. CoA_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP37766.

Entry information

Entry nameYDIF_ECOLI
AccessionPrimary (citable) accession number: P37766
Secondary accession number(s): P76199, P76898
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene