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P37765

- RLUB_ECOLI

UniProt

P37765 - RLUB_ECOLI

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Protein

Ribosomal large subunit pseudouridine synthase B

Gene

rluB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA.1 Publication

Catalytic activityi

23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei110 – 1101NucleophileCurated

GO - Molecular functioni

  1. pseudouridine synthase activity Source: EcoCyc
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12433-MONOMER.
ECOL316407:JW1261-MONOMER.
MetaCyc:EG12433-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal large subunit pseudouridine synthase B (EC:5.4.99.22)
Alternative name(s):
23S rRNA pseudouridine(2605) synthase
rRNA pseudouridylate synthase B
rRNA-uridine isomerase B
Gene namesi
Name:rluB
Synonyms:yciL
Ordered Locus Names:b1269, JW1261
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12433. rluB.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi110 – 1101D → N or T: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Ribosomal large subunit pseudouridine synthase BPRO_0000099983Add
BLAST

Proteomic databases

PaxDbiP37765.
PRIDEiP37765.

Expressioni

Gene expression databases

GenevestigatoriP37765.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
rpsGP023593EBI-561550,EBI-543074
srmBP215074EBI-561550,EBI-546628

Protein-protein interaction databases

DIPiDIP-11585N.
IntActiP37765. 76 interactions.
STRINGi511145.b1269.

Structurei

Secondary structure

1
291
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 117
Helixi17 – 259
Beta strandi29 – 313
Beta strandi50 – 523
Beta strandi69 – 746
Turni91 – 944
Beta strandi99 – 1013
Beta strandi104 – 1074
Beta strandi114 – 1218
Helixi123 – 1308
Helixi132 – 1343
Beta strandi138 – 1469
Helixi150 – 1589
Beta strandi160 – 1623
Beta strandi165 – 1673
Beta strandi170 – 1789
Beta strandi181 – 19010
Helixi195 – 2028
Beta strandi206 – 21510
Beta strandi229 – 2313
Helixi234 – 24310

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LABX-ray2.50A1-251[»]
4LGTX-ray1.30A/D1-251[»]
ProteinModelPortaliP37765.
SMRiP37765. Positions 1-251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 7573S4 RNA-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the pseudouridine synthase RsuA family.Curated
Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1187.
HOGENOMiHOG000044954.
InParanoidiP37765.
KOiK06178.
OMAiARSQKIR.
OrthoDBiEOG6130DV.
PhylomeDBiP37765.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiPS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37765-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIAKLGD RVEVTPGLKI
60 70 80 90 100
RIDGHLISVR ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG
110 120 130 140 150
ARWIAVGRLD VNTCGLLLFT TDGELANRLM HPSREVEREY AVRVFGQVDD
160 170 180 190 200
AKLRDLSRGV QLEDGPAAFK TIKFSGGEGI NQWYNVTLTE GRNREVRRLW
210 220 230 240 250
EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL RELVELPPET
260 270 280 290
SSKVAVEKDR RRMKANQIRR AVKRHSQVSG GRRSGGRNNN G
Length:291
Mass (Da):32,711
Last modified:November 1, 1995 - v2
Checksum:i101DF8FD55CB557D
GO

Sequence cautioni

The sequence M21528 differs from that shown. Reason: Frameshift at position 228.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74351.1.
AP009048 Genomic DNA. Translation: BAA14806.1.
U18111 Genomic DNA. Translation: AAB59990.1.
M21528 Genomic DNA. No translation available.
PIRiH64874.
RefSeqiNP_415785.1. NC_000913.3.
YP_489537.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74351; AAC74351; b1269.
BAA14806; BAA14806; BAA14806.
GeneIDi12930580.
945840.
KEGGiecj:Y75_p1243.
eco:b1269.
PATRICi32117796. VBIEscCol129921_1318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74351.1 .
AP009048 Genomic DNA. Translation: BAA14806.1 .
U18111 Genomic DNA. Translation: AAB59990.1 .
M21528 Genomic DNA. No translation available.
PIRi H64874.
RefSeqi NP_415785.1. NC_000913.3.
YP_489537.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4LAB X-ray 2.50 A 1-251 [» ]
4LGT X-ray 1.30 A/D 1-251 [» ]
ProteinModelPortali P37765.
SMRi P37765. Positions 1-251.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-11585N.
IntActi P37765. 76 interactions.
STRINGi 511145.b1269.

Proteomic databases

PaxDbi P37765.
PRIDEi P37765.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74351 ; AAC74351 ; b1269 .
BAA14806 ; BAA14806 ; BAA14806 .
GeneIDi 12930580.
945840.
KEGGi ecj:Y75_p1243.
eco:b1269.
PATRICi 32117796. VBIEscCol129921_1318.

Organism-specific databases

EchoBASEi EB2329.
EcoGenei EG12433. rluB.

Phylogenomic databases

eggNOGi COG1187.
HOGENOMi HOG000044954.
InParanoidi P37765.
KOi K06178.
OMAi ARSQKIR.
OrthoDBi EOG6130DV.
PhylomeDBi P37765.

Enzyme and pathway databases

BioCyci EcoCyc:EG12433-MONOMER.
ECOL316407:JW1261-MONOMER.
MetaCyc:EG12433-MONOMER.

Miscellaneous databases

PROi P37765.

Gene expression databases

Genevestigatori P37765.

Family and domain databases

Gene3Di 3.10.290.10. 1 hit.
InterProi IPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view ]
Pfami PF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view ]
SMARTi SM00363. S4. 1 hit.
[Graphical view ]
SUPFAMi SSF55120. SSF55120. 1 hit.
TIGRFAMsi TIGR00093. TIGR00093. 1 hit.
PROSITEi PS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Milkman R., McKane M.
    Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-243.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Altered cobalamin metabolism in Escherichia coli btuR mutants affects btuB gene regulation."
    Lundrigan M.D., Kadner R.J.
    J. Bacteriol. 171:154-161(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 217-291.
    Strain: K12.
  6. "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
    Borodovsky M., Rudd K.E., Koonin E.V.
    Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  7. "Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli."
    Del Campo M., Kaya Y., Ofengand J.
    RNA 7:1603-1615(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-110.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiRLUB_ECOLI
AccessioniPrimary (citable) accession number: P37765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3