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Protein

Ribosomal large subunit pseudouridine synthase B

Gene

rluB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA.1 Publication

Catalytic activityi

23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei110 – 1101NucleophileCurated

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12433-MONOMER.
ECOL316407:JW1261-MONOMER.
MetaCyc:EG12433-MONOMER.
BRENDAi5.4.99.22. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal large subunit pseudouridine synthase B (EC:5.4.99.22)
Alternative name(s):
23S rRNA pseudouridine(2605) synthase
rRNA pseudouridylate synthase B
rRNA-uridine isomerase B
Gene namesi
Name:rluB
Synonyms:yciL
Ordered Locus Names:b1269, JW1261
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12433. rluB.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi110 – 1101D → N or T: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Ribosomal large subunit pseudouridine synthase BPRO_0000099983Add
BLAST

Proteomic databases

PaxDbiP37765.
PRIDEiP37765.

Expressioni

Gene expression databases

GenevestigatoriP37765.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
rpsGP023593EBI-561550,EBI-543074
srmBP215074EBI-561550,EBI-546628

Protein-protein interaction databases

DIPiDIP-11585N.
IntActiP37765. 76 interactions.
STRINGi511145.b1269.

Structurei

Secondary structure

1
291
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 117Combined sources
Helixi17 – 259Combined sources
Beta strandi29 – 313Combined sources
Beta strandi50 – 523Combined sources
Beta strandi69 – 746Combined sources
Turni91 – 944Combined sources
Beta strandi99 – 1013Combined sources
Beta strandi104 – 1074Combined sources
Beta strandi114 – 1218Combined sources
Helixi123 – 1308Combined sources
Helixi132 – 1343Combined sources
Beta strandi138 – 1469Combined sources
Helixi150 – 1589Combined sources
Beta strandi160 – 1623Combined sources
Beta strandi165 – 1673Combined sources
Beta strandi170 – 1789Combined sources
Beta strandi181 – 19010Combined sources
Helixi195 – 2028Combined sources
Beta strandi206 – 21510Combined sources
Beta strandi229 – 2313Combined sources
Helixi234 – 24310Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LABX-ray2.50A1-251[»]
4LGTX-ray1.30A/D1-251[»]
ProteinModelPortaliP37765.
SMRiP37765. Positions 1-251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 7573S4 RNA-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the pseudouridine synthase RsuA family.Curated
Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1187.
HOGENOMiHOG000044954.
InParanoidiP37765.
KOiK06178.
OMAiTKANQIR.
OrthoDBiEOG6130DV.
PhylomeDBiP37765.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiPS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37765-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIAKLGD RVEVTPGLKI
60 70 80 90 100
RIDGHLISVR ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG
110 120 130 140 150
ARWIAVGRLD VNTCGLLLFT TDGELANRLM HPSREVEREY AVRVFGQVDD
160 170 180 190 200
AKLRDLSRGV QLEDGPAAFK TIKFSGGEGI NQWYNVTLTE GRNREVRRLW
210 220 230 240 250
EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL RELVELPPET
260 270 280 290
SSKVAVEKDR RRMKANQIRR AVKRHSQVSG GRRSGGRNNN G
Length:291
Mass (Da):32,711
Last modified:November 1, 1995 - v2
Checksum:i101DF8FD55CB557D
GO

Sequence cautioni

The sequence M21528 differs from that shown. Reason: Frameshift at position 228. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74351.1.
AP009048 Genomic DNA. Translation: BAA14806.1.
U18111 Genomic DNA. Translation: AAB59990.1.
M21528 Genomic DNA. No translation available.
PIRiH64874.
RefSeqiNP_415785.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74351; AAC74351; b1269.
BAA14806; BAA14806; BAA14806.
GeneIDi945840.
KEGGiecj:Y75_p1243.
eco:b1269.
PATRICi32117796. VBIEscCol129921_1318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74351.1.
AP009048 Genomic DNA. Translation: BAA14806.1.
U18111 Genomic DNA. Translation: AAB59990.1.
M21528 Genomic DNA. No translation available.
PIRiH64874.
RefSeqiNP_415785.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LABX-ray2.50A1-251[»]
4LGTX-ray1.30A/D1-251[»]
ProteinModelPortaliP37765.
SMRiP37765. Positions 1-251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-11585N.
IntActiP37765. 76 interactions.
STRINGi511145.b1269.

Proteomic databases

PaxDbiP37765.
PRIDEiP37765.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74351; AAC74351; b1269.
BAA14806; BAA14806; BAA14806.
GeneIDi945840.
KEGGiecj:Y75_p1243.
eco:b1269.
PATRICi32117796. VBIEscCol129921_1318.

Organism-specific databases

EchoBASEiEB2329.
EcoGeneiEG12433. rluB.

Phylogenomic databases

eggNOGiCOG1187.
HOGENOMiHOG000044954.
InParanoidiP37765.
KOiK06178.
OMAiTKANQIR.
OrthoDBiEOG6130DV.
PhylomeDBiP37765.

Enzyme and pathway databases

BioCyciEcoCyc:EG12433-MONOMER.
ECOL316407:JW1261-MONOMER.
MetaCyc:EG12433-MONOMER.
BRENDAi5.4.99.22. 2026.

Miscellaneous databases

PROiP37765.

Gene expression databases

GenevestigatoriP37765.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiPS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Milkman R., McKane M.
    Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-243.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Altered cobalamin metabolism in Escherichia coli btuR mutants affects btuB gene regulation."
    Lundrigan M.D., Kadner R.J.
    J. Bacteriol. 171:154-161(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 217-291.
    Strain: K12.
  6. "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
    Borodovsky M., Rudd K.E., Koonin E.V.
    Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  7. "Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli."
    Del Campo M., Kaya Y., Ofengand J.
    RNA 7:1603-1615(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-110.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiRLUB_ECOLI
AccessioniPrimary (citable) accession number: P37765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1995
Last modified: May 27, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.