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Protein

Ribosomal large subunit pseudouridine synthase B

Gene

rluB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA.1 Publication

Catalytic activityi

23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei110NucleophileCurated1

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12433-MONOMER.
ECOL316407:JW1261-MONOMER.
MetaCyc:EG12433-MONOMER.
BRENDAi5.4.99.22. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal large subunit pseudouridine synthase B (EC:5.4.99.22)
Alternative name(s):
23S rRNA pseudouridine(2605) synthase
rRNA pseudouridylate synthase B
rRNA-uridine isomerase B
Gene namesi
Name:rluB
Synonyms:yciL
Ordered Locus Names:b1269, JW1261
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12433. rluB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi110D → N or T: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000999831 – 291Ribosomal large subunit pseudouridine synthase BAdd BLAST291

Proteomic databases

PaxDbiP37765.
PRIDEiP37765.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
rpsGP023593EBI-561550,EBI-543074
srmBP215074EBI-561550,EBI-546628

Protein-protein interaction databases

BioGridi4259574. 10 interactors.
DIPiDIP-11585N.
IntActiP37765. 76 interactors.
STRINGi511145.b1269.

Structurei

Secondary structure

1291
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 11Combined sources7
Helixi17 – 25Combined sources9
Beta strandi29 – 31Combined sources3
Beta strandi50 – 52Combined sources3
Beta strandi69 – 74Combined sources6
Turni91 – 94Combined sources4
Beta strandi99 – 101Combined sources3
Beta strandi104 – 107Combined sources4
Beta strandi114 – 121Combined sources8
Helixi123 – 130Combined sources8
Helixi132 – 134Combined sources3
Beta strandi138 – 146Combined sources9
Helixi150 – 158Combined sources9
Beta strandi160 – 162Combined sources3
Beta strandi165 – 167Combined sources3
Beta strandi170 – 178Combined sources9
Beta strandi181 – 190Combined sources10
Helixi195 – 202Combined sources8
Beta strandi206 – 215Combined sources10
Beta strandi229 – 231Combined sources3
Helixi234 – 243Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LABX-ray2.50A1-251[»]
4LGTX-ray1.30A/D1-251[»]
ProteinModelPortaliP37765.
SMRiP37765.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 75S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST73

Sequence similaritiesi

Belongs to the pseudouridine synthase RsuA family.Curated
Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105FE9. Bacteria.
COG1187. LUCA.
HOGENOMiHOG000044954.
InParanoidiP37765.
KOiK06178.
OMAiQKEICRV.
PhylomeDBiP37765.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiPS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37765-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIAKLGD RVEVTPGLKI
60 70 80 90 100
RIDGHLISVR ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG
110 120 130 140 150
ARWIAVGRLD VNTCGLLLFT TDGELANRLM HPSREVEREY AVRVFGQVDD
160 170 180 190 200
AKLRDLSRGV QLEDGPAAFK TIKFSGGEGI NQWYNVTLTE GRNREVRRLW
210 220 230 240 250
EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL RELVELPPET
260 270 280 290
SSKVAVEKDR RRMKANQIRR AVKRHSQVSG GRRSGGRNNN G
Length:291
Mass (Da):32,711
Last modified:November 1, 1995 - v2
Checksum:i101DF8FD55CB557D
GO

Sequence cautioni

The sequence M21528 differs from that shown. Reason: Frameshift at position 228.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74351.1.
AP009048 Genomic DNA. Translation: BAA14806.1.
U18111 Genomic DNA. Translation: AAB59990.1.
M21528 Genomic DNA. No translation available.
PIRiH64874.
RefSeqiNP_415785.1. NC_000913.3.
WP_001291217.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74351; AAC74351; b1269.
BAA14806; BAA14806; BAA14806.
GeneIDi945840.
KEGGiecj:JW1261.
eco:b1269.
PATRICi32117796. VBIEscCol129921_1318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74351.1.
AP009048 Genomic DNA. Translation: BAA14806.1.
U18111 Genomic DNA. Translation: AAB59990.1.
M21528 Genomic DNA. No translation available.
PIRiH64874.
RefSeqiNP_415785.1. NC_000913.3.
WP_001291217.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LABX-ray2.50A1-251[»]
4LGTX-ray1.30A/D1-251[»]
ProteinModelPortaliP37765.
SMRiP37765.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259574. 10 interactors.
DIPiDIP-11585N.
IntActiP37765. 76 interactors.
STRINGi511145.b1269.

Proteomic databases

PaxDbiP37765.
PRIDEiP37765.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74351; AAC74351; b1269.
BAA14806; BAA14806; BAA14806.
GeneIDi945840.
KEGGiecj:JW1261.
eco:b1269.
PATRICi32117796. VBIEscCol129921_1318.

Organism-specific databases

EchoBASEiEB2329.
EcoGeneiEG12433. rluB.

Phylogenomic databases

eggNOGiENOG4105FE9. Bacteria.
COG1187. LUCA.
HOGENOMiHOG000044954.
InParanoidiP37765.
KOiK06178.
OMAiQKEICRV.
PhylomeDBiP37765.

Enzyme and pathway databases

BioCyciEcoCyc:EG12433-MONOMER.
ECOL316407:JW1261-MONOMER.
MetaCyc:EG12433-MONOMER.
BRENDAi5.4.99.22. 2026.

Miscellaneous databases

PROiP37765.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiPS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRLUB_ECOLI
AccessioniPrimary (citable) accession number: P37765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.