P37765 (RLUB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal large subunit pseudouridine synthase B
EC=5.4.99.22

Alternative name(s):
23S rRNA pseudouridine(2605) synthase
rRNA pseudouridylate synthase B
rRNA-uridine isomerase B

Gene names

Name:	rluB
Synonyms:	yciL
Ordered Locus Names:	b1269, JW1261

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA. Ref.7
Catalytic activity	23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605). Ref.7
Sequence similarities	Belongs to the pseudouridine synthase RsuA family. Contains 1 S4 RNA-binding domain.
Sequence caution	The sequence M21528 differs from that shown. Reason: Frameshift at position 228.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	RNA-binding
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	enzyme-directed rRNA pseudouridine synthesis Inferred from mutant phenotype Ref.7. Source: EcoCyc
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from mutant phenotype Ref.7. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Ribosomal large subunit pseudouridine synthase B		PRO_0000099983
Regions
	Domain	3 – 75	73	S4 RNA-binding
Sites
	Active site	110	1	Nucleophile Probable
Experimental info
	Mutagenesis	110	1	D → N or T: Loss of activity. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P37765 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 101DF8FD55CB557D	FASTA	291	32,711
	10 20 30 40 50 60 MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIAKLGD RVEVTPGLKI RIDGHLISVR 70 80 90 100 110 120 ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG ARWIAVGRLD VNTCGLLLFT 130 140 150 160 170 180 TDGELANRLM HPSREVEREY AVRVFGQVDD AKLRDLSRGV QLEDGPAAFK TIKFSGGEGI 190 200 210 220 230 240 NQWYNVTLTE GRNREVRRLW EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL 250 260 270 280 290 RELVELPPET SSKVAVEKDR RRMKANQIRR AVKRHSQVSG GRRSGGRNNN G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	Milkman R., McKane M. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-243. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Altered cobalamin metabolism in Escherichia coli btuR mutants affects btuB gene regulation." Lundrigan M.D., Kadner R.J. J. Bacteriol. 171:154-161(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 217-291. Strain: K12.
[6]	"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Borodovsky M., Rudd K.E., Koonin E.V. Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[7]	"Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli." Del Campo M., Kaya Y., Ofengand J. RNA 7:1603-1615(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-110. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74351.1. AP009048 Genomic DNA. Translation: BAA14806.1. U18111 Genomic DNA. Translation: AAB59990.1. M21528 Genomic DNA. No translation available.
PIR	H64874.
RefSeq	NP_415785.1. NC_000913.2. YP_489537.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P37765.
SMR	P37765. Positions 2-254.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11585N.
IntAct	P37765. 76 interactions.
STRING	511145.b1269.
Proteomic databases
PaxDb	P37765.
PRIDE	P37765.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74351; AAC74351; b1269. BAA14806; BAA14806; BAA14806.
GeneID	12930580. 945840.
KEGG	ecj:Y75_p1243. eco:b1269.
PATRIC	32117796. VBIEscCol129921_1318.
Organism-specific databases
EchoBASE	EB2329.
EcoGene	EG12433. rluB.
Phylogenomic databases
eggNOG	COG1187.
HOGENOM	HOG000044954.
KO	K06178.
OMA	PRGGWIE.
ProtClustDB	PRK10700.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12433-MONOMER. ECOL316407:JW1261-MONOMER. MetaCyc:EG12433-MONOMER.
Gene expression databases
Genevestigator	P37765.
Family and domain databases
Gene3D	3.10.290.10. 1 hit.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR000748. PsdUridine_synth_RsuA/RluB/E/F. IPR018496. PsdUridine_synth_RsuA/RluB_CS. IPR006145. PsdUridine_synth_RsuA/RluD. IPR002942. S4_RNA-bd. [Graphical view]
Pfam	PF00849. PseudoU_synth_2. 1 hit. PF01479. S4. 1 hit. [Graphical view]
SMART	SM00363. S4. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00093. TIGR00093. 1 hit.
PROSITE	PS01149. PSI_RSU. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLUB_ECOLI

Accession

Primary (citable) accession number: P37765

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program