Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosomal large subunit pseudouridine synthase B

Gene

rluB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA.1 Publication

Catalytic activityi

23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei110NucleophileCurated1

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc

Keywordsi

Molecular functionIsomerase, RNA-binding
Biological processrRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG12433-MONOMER
MetaCyc:EG12433-MONOMER
BRENDAi5.4.99.22 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal large subunit pseudouridine synthase B (EC:5.4.99.22)
Alternative name(s):
23S rRNA pseudouridine(2605) synthase
rRNA pseudouridylate synthase B
rRNA-uridine isomerase B
Gene namesi
Name:rluB
Synonyms:yciL
Ordered Locus Names:b1269, JW1261
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12433 rluB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi110D → N or T: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000999831 – 291Ribosomal large subunit pseudouridine synthase BAdd BLAST291

Proteomic databases

PaxDbiP37765
PRIDEiP37765

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4259574, 50 interactors
DIPiDIP-11585N
IntActiP37765, 79 interactors
STRINGi316385.ECDH10B_1384

Structurei

Secondary structure

1291
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 11Combined sources7
Helixi17 – 25Combined sources9
Beta strandi29 – 31Combined sources3
Beta strandi50 – 52Combined sources3
Beta strandi69 – 74Combined sources6
Turni91 – 94Combined sources4
Beta strandi99 – 101Combined sources3
Beta strandi104 – 107Combined sources4
Beta strandi114 – 121Combined sources8
Helixi123 – 130Combined sources8
Helixi132 – 134Combined sources3
Beta strandi138 – 146Combined sources9
Helixi150 – 158Combined sources9
Beta strandi160 – 162Combined sources3
Beta strandi165 – 167Combined sources3
Beta strandi170 – 178Combined sources9
Beta strandi181 – 190Combined sources10
Helixi195 – 202Combined sources8
Beta strandi206 – 215Combined sources10
Beta strandi229 – 231Combined sources3
Helixi234 – 243Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LABX-ray2.50A1-251[»]
4LGTX-ray1.30A/D1-251[»]
ProteinModelPortaliP37765
SMRiP37765
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 75S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST73

Sequence similaritiesi

Belongs to the pseudouridine synthase RsuA family.Curated

Phylogenomic databases

eggNOGiENOG4105FE9 Bacteria
COG1187 LUCA
HOGENOMiHOG000044954
InParanoidiP37765
KOiK06178
OMAiSMEFAPF
PhylomeDBiP37765

Family and domain databases

CDDicd00165 S4, 1 hit
Gene3Di3.10.290.10, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR000748 PsdUridine_synth_RsuA/RluB/E/F
IPR018496 PsdUridine_synth_RsuA/RluB_CS
IPR006145 PsdUridine_synth_RsuA/RluD
IPR002942 S4_RNA-bd
IPR036986 S4_RNA-bd_sf
PANTHERiPTHR21600 PTHR21600, 1 hit
PfamiView protein in Pfam
PF00849 PseudoU_synth_2, 1 hit
PF01479 S4, 1 hit
SMARTiView protein in SMART
SM00363 S4, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit
TIGRFAMsiTIGR00093 TIGR00093, 1 hit
PROSITEiView protein in PROSITE
PS01149 PSI_RSU, 1 hit
PS50889 S4, 1 hit

Sequencei

Sequence statusi: Complete.

P37765-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIAKLGD RVEVTPGLKI
60 70 80 90 100
RIDGHLISVR ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG
110 120 130 140 150
ARWIAVGRLD VNTCGLLLFT TDGELANRLM HPSREVEREY AVRVFGQVDD
160 170 180 190 200
AKLRDLSRGV QLEDGPAAFK TIKFSGGEGI NQWYNVTLTE GRNREVRRLW
210 220 230 240 250
EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL RELVELPPET
260 270 280 290
SSKVAVEKDR RRMKANQIRR AVKRHSQVSG GRRSGGRNNN G
Length:291
Mass (Da):32,711
Last modified:November 1, 1995 - v2
Checksum:i101DF8FD55CB557D
GO

Sequence cautioni

The sequence M21528 differs from that shown. Reason: Frameshift at position 228.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74351.1
AP009048 Genomic DNA Translation: BAA14806.1
U18111 Genomic DNA Translation: AAB59990.1
M21528 Genomic DNA No translation available.
PIRiH64874
RefSeqiNP_415785.1, NC_000913.3
WP_001291217.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74351; AAC74351; b1269
BAA14806; BAA14806; BAA14806
GeneIDi945840
KEGGiecj:JW1261
eco:b1269
PATRICifig|1411691.4.peg.1015

Similar proteinsi

Entry informationi

Entry nameiRLUB_ECOLI
AccessioniPrimary (citable) accession number: P37765
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health