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P37765 (RLUB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal large subunit pseudouridine synthase B

EC=5.4.99.22
Alternative name(s):
23S rRNA pseudouridine(2605) synthase
rRNA pseudouridylate synthase B
rRNA-uridine isomerase B
Gene names
Name:rluB
Synonyms:yciL
Ordered Locus Names:b1269, JW1261
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA. Ref.7

Catalytic activity

23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605). Ref.7

Sequence similarities

Belongs to the pseudouridine synthase RsuA family.

Contains 1 S4 RNA-binding domain.

Sequence caution

The sequence M21528 differs from that shown. Reason: Frameshift at position 228.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Ribosomal large subunit pseudouridine synthase B
PRO_0000099983

Regions

Domain3 – 7573S4 RNA-binding

Sites

Active site1101Nucleophile Probable

Experimental info

Mutagenesis1101D → N or T: Loss of activity. Ref.7

Secondary structure

........................................... 291
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P37765 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 101DF8FD55CB557D

FASTA29132,711
        10         20         30         40         50         60 
MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIAKLGD RVEVTPGLKI RIDGHLISVR 

        70         80         90        100        110        120 
ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG ARWIAVGRLD VNTCGLLLFT 

       130        140        150        160        170        180 
TDGELANRLM HPSREVEREY AVRVFGQVDD AKLRDLSRGV QLEDGPAAFK TIKFSGGEGI 

       190        200        210        220        230        240 
NQWYNVTLTE GRNREVRRLW EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL 

       250        260        270        280        290 
RELVELPPET SSKVAVEKDR RRMKANQIRR AVKRHSQVSG GRRSGGRNNN G 

« Hide

References

« Hide 'large scale' references
[1]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]Milkman R., McKane M.
Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-243.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Altered cobalamin metabolism in Escherichia coli btuR mutants affects btuB gene regulation."
Lundrigan M.D., Kadner R.J.
J. Bacteriol. 171:154-161(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 217-291.
Strain: K12.
[6]"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
Borodovsky M., Rudd K.E., Koonin E.V.
Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[7]"Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli."
Del Campo M., Kaya Y., Ofengand J.
RNA 7:1603-1615(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-110.
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74351.1.
AP009048 Genomic DNA. Translation: BAA14806.1.
U18111 Genomic DNA. Translation: AAB59990.1.
M21528 Genomic DNA. No translation available.
PIRH64874.
RefSeqNP_415785.1. NC_000913.3.
YP_489537.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4LABX-ray2.50A1-251[»]
4LGTX-ray1.30A/D1-251[»]
ProteinModelPortalP37765.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-11585N.
IntActP37765. 76 interactions.
STRING511145.b1269.

Proteomic databases

PaxDbP37765.
PRIDEP37765.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74351; AAC74351; b1269.
BAA14806; BAA14806; BAA14806.
GeneID12930580.
945840.
KEGGecj:Y75_p1243.
eco:b1269.
PATRIC32117796. VBIEscCol129921_1318.

Organism-specific databases

EchoBASEEB2329.
EcoGeneEG12433. rluB.

Phylogenomic databases

eggNOGCOG1187.
HOGENOMHOG000044954.
KOK06178.
OMAARSQKIR.
OrthoDBEOG6130DV.
PhylomeDBP37765.

Enzyme and pathway databases

BioCycEcoCyc:EG12433-MONOMER.
ECOL316407:JW1261-MONOMER.
MetaCyc:EG12433-MONOMER.

Gene expression databases

GenevestigatorP37765.

Family and domain databases

Gene3D3.10.290.10. 1 hit.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTSM00363. S4. 1 hit.
[Graphical view]
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR00093. TIGR00093. 1 hit.
PROSITEPS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP37765.

Entry information

Entry nameRLUB_ECOLI
AccessionPrimary (citable) accession number: P37765
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene