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P37765

- RLUB_ECOLI

UniProt

P37765 - RLUB_ECOLI

Protein

Ribosomal large subunit pseudouridine synthase B

Gene

rluB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA.1 Publication

    Catalytic activityi

    23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei110 – 1101NucleophileCurated

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. pseudouridine synthase activity Source: EcoCyc
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    rRNA processing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG12433-MONOMER.
    ECOL316407:JW1261-MONOMER.
    MetaCyc:EG12433-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosomal large subunit pseudouridine synthase B (EC:5.4.99.22)
    Alternative name(s):
    23S rRNA pseudouridine(2605) synthase
    rRNA pseudouridylate synthase B
    rRNA-uridine isomerase B
    Gene namesi
    Name:rluB
    Synonyms:yciL
    Ordered Locus Names:b1269, JW1261
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12433. rluB.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi110 – 1101D → N or T: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 291291Ribosomal large subunit pseudouridine synthase BPRO_0000099983Add
    BLAST

    Proteomic databases

    PaxDbiP37765.
    PRIDEiP37765.

    Expressioni

    Gene expression databases

    GenevestigatoriP37765.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    rpsGP023593EBI-561550,EBI-543074
    srmBP215074EBI-561550,EBI-546628

    Protein-protein interaction databases

    DIPiDIP-11585N.
    IntActiP37765. 76 interactions.
    STRINGi511145.b1269.

    Structurei

    Secondary structure

    1
    291
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 117
    Helixi17 – 259
    Beta strandi29 – 313
    Beta strandi50 – 523
    Beta strandi69 – 746
    Turni91 – 944
    Beta strandi99 – 1013
    Beta strandi104 – 1074
    Beta strandi114 – 1218
    Helixi123 – 1308
    Helixi132 – 1343
    Beta strandi138 – 1469
    Helixi150 – 1589
    Beta strandi160 – 1623
    Beta strandi165 – 1673
    Beta strandi170 – 1789
    Beta strandi181 – 19010
    Helixi195 – 2028
    Beta strandi206 – 21510
    Beta strandi229 – 2313
    Helixi234 – 24310

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4LABX-ray2.50A1-251[»]
    4LGTX-ray1.30A/D1-251[»]
    ProteinModelPortaliP37765.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 7573S4 RNA-bindingPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the pseudouridine synthase RsuA family.Curated
    Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1187.
    HOGENOMiHOG000044954.
    KOiK06178.
    OMAiARSQKIR.
    OrthoDBiEOG6130DV.
    PhylomeDBiP37765.

    Family and domain databases

    Gene3Di3.10.290.10. 1 hit.
    InterProiIPR020103. PsdUridine_synth_cat_dom.
    IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
    IPR018496. PsdUridine_synth_RsuA/RluB_CS.
    IPR006145. PsdUridine_synth_RsuA/RluD.
    IPR002942. S4_RNA-bd.
    [Graphical view]
    PfamiPF00849. PseudoU_synth_2. 1 hit.
    PF01479. S4. 1 hit.
    [Graphical view]
    SMARTiSM00363. S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF55120. SSF55120. 1 hit.
    TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
    PROSITEiPS01149. PSI_RSU. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P37765-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIAKLGD RVEVTPGLKI    50
    RIDGHLISVR ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG 100
    ARWIAVGRLD VNTCGLLLFT TDGELANRLM HPSREVEREY AVRVFGQVDD 150
    AKLRDLSRGV QLEDGPAAFK TIKFSGGEGI NQWYNVTLTE GRNREVRRLW 200
    EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL RELVELPPET 250
    SSKVAVEKDR RRMKANQIRR AVKRHSQVSG GRRSGGRNNN G 291
    Length:291
    Mass (Da):32,711
    Last modified:November 1, 1995 - v2
    Checksum:i101DF8FD55CB557D
    GO

    Sequence cautioni

    The sequence M21528 differs from that shown. Reason: Frameshift at position 228.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74351.1.
    AP009048 Genomic DNA. Translation: BAA14806.1.
    U18111 Genomic DNA. Translation: AAB59990.1.
    M21528 Genomic DNA. No translation available.
    PIRiH64874.
    RefSeqiNP_415785.1. NC_000913.3.
    YP_489537.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74351; AAC74351; b1269.
    BAA14806; BAA14806; BAA14806.
    GeneIDi12930580.
    945840.
    KEGGiecj:Y75_p1243.
    eco:b1269.
    PATRICi32117796. VBIEscCol129921_1318.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74351.1 .
    AP009048 Genomic DNA. Translation: BAA14806.1 .
    U18111 Genomic DNA. Translation: AAB59990.1 .
    M21528 Genomic DNA. No translation available.
    PIRi H64874.
    RefSeqi NP_415785.1. NC_000913.3.
    YP_489537.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4LAB X-ray 2.50 A 1-251 [» ]
    4LGT X-ray 1.30 A/D 1-251 [» ]
    ProteinModelPortali P37765.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-11585N.
    IntActi P37765. 76 interactions.
    STRINGi 511145.b1269.

    Proteomic databases

    PaxDbi P37765.
    PRIDEi P37765.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74351 ; AAC74351 ; b1269 .
    BAA14806 ; BAA14806 ; BAA14806 .
    GeneIDi 12930580.
    945840.
    KEGGi ecj:Y75_p1243.
    eco:b1269.
    PATRICi 32117796. VBIEscCol129921_1318.

    Organism-specific databases

    EchoBASEi EB2329.
    EcoGenei EG12433. rluB.

    Phylogenomic databases

    eggNOGi COG1187.
    HOGENOMi HOG000044954.
    KOi K06178.
    OMAi ARSQKIR.
    OrthoDBi EOG6130DV.
    PhylomeDBi P37765.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG12433-MONOMER.
    ECOL316407:JW1261-MONOMER.
    MetaCyc:EG12433-MONOMER.

    Miscellaneous databases

    PROi P37765.

    Gene expression databases

    Genevestigatori P37765.

    Family and domain databases

    Gene3Di 3.10.290.10. 1 hit.
    InterProi IPR020103. PsdUridine_synth_cat_dom.
    IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
    IPR018496. PsdUridine_synth_RsuA/RluB_CS.
    IPR006145. PsdUridine_synth_RsuA/RluD.
    IPR002942. S4_RNA-bd.
    [Graphical view ]
    Pfami PF00849. PseudoU_synth_2. 1 hit.
    PF01479. S4. 1 hit.
    [Graphical view ]
    SMARTi SM00363. S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55120. SSF55120. 1 hit.
    TIGRFAMsi TIGR00093. TIGR00093. 1 hit.
    PROSITEi PS01149. PSI_RSU. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Milkman R., McKane M.
      Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-243.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Altered cobalamin metabolism in Escherichia coli btuR mutants affects btuB gene regulation."
      Lundrigan M.D., Kadner R.J.
      J. Bacteriol. 171:154-161(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 217-291.
      Strain: K12.
    6. "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
      Borodovsky M., Rudd K.E., Koonin E.V.
      Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    7. "Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli."
      Del Campo M., Kaya Y., Ofengand J.
      RNA 7:1603-1615(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-110.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiRLUB_ECOLI
    AccessioniPrimary (citable) accession number: P37765
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3