Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

dTDP-4-dehydrorhamnose reductase

Gene

rfbD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well (By similarity).By similarity

Catalytic activityi

dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: dTDP-L-rhamnose biosynthesis

This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12NAD; via amide nitrogenBy similarity1
Binding sitei102NADP; via carbonyl oxygenBy similarity1
Binding sitei153Substrate; via amide nitrogenBy similarity1
Binding sitei154NAD; via amide nitrogenBy similarity1
Binding sitei223SubstrateBy similarity1
Binding sitei260SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 11NADBy similarity5
Nucleotide bindingi11 – 12NADPBy similarity2
Nucleotide bindingi30 – 31NADBy similarity2
Nucleotide bindingi39 – 40NAD/NADPBy similarity2
Nucleotide bindingi62 – 65NADBy similarity4
Nucleotide bindingi63 – 65NADPBy similarity3
Nucleotide bindingi128 – 132NAD/NADPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, NADP

Enzyme and pathway databases

BioCyciEcoCyc:DTDPDEHYRHAMREDUCT-MONOMER.
ECOL316407:JW2025-MONOMER.
MetaCyc:DTDPDEHYRHAMREDUCT-MONOMER.
UniPathwayiUPA00124.
UPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-4-dehydrorhamnose reductase (EC:1.1.1.133)
Alternative name(s):
dTDP-4-keto-L-rhamnose reductase
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
dTDP-6-deoxy-L-mannose dehydrogenase
dTDP-L-rhamnose synthase
Gene namesi
Name:rfbD
Synonyms:rmlD
Ordered Locus Names:b2040, JW2025
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12411. rfbD.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002079841 – 299dTDP-4-dehydrorhamnose reductaseAdd BLAST299

Proteomic databases

PaxDbiP37760.
PRIDEiP37760.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4261549. 162 interactors.
IntActiP37760. 5 interactors.
STRINGi511145.b2040.

Structurei

3D structure databases

ProteinModelPortaliP37760.
SMRiP37760.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 105Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DBZ. Bacteria.
COG1091. LUCA.
HOGENOMiHOG000227712.
InParanoidiP37760.
KOiK00067.
OMAiGYASRYE.
PhylomeDBiP37760.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
IPR029903. RmlD-like-bd.
[Graphical view]
PANTHERiPTHR10491. PTHR10491. 1 hit.
PfamiPF04321. RmlD_sub_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01214. rmlD. 1 hit.

Sequencei

Sequence statusi: Complete.

P37760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNILLFGKTG QVGWELQRAL APLGNLIAFD VHSTDYCGDF SNPEGVAETV
60 70 80 90 100
RSIRPDIIVN AAAHTAVDKA ESEPEFAQLI NATSVEAIAK AANEVGAWVI
110 120 130 140 150
HYSTDYVFPG NGDMPWLETD ATAPLNVYGE TKLAGEKALQ EYCAKHLIFR
160 170 180 190 200
TSWVYAGKGN NFAKTMLRLA KEREELAVIN DQFGAPTGAE LLADCTAHAI
210 220 230 240 250
RVALNKPDVA GLYHLVASGT TTWYDYAALV FEEARKAGIP LALNKLNAVP
260 270 280 290
TTAYPTPARR PHNSRLNTEK FQQNFALVLP DWQVGVKRML NELFTTTAI
Length:299
Mass (Da):32,694
Last modified:November 1, 1997 - v2
Checksum:i1D7C992FA5017AD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09876 Genomic DNA. Translation: AAB88399.1.
U00096 Genomic DNA. Translation: AAC75101.1.
AP009048 Genomic DNA. Translation: BAA15882.1.
PIRiG64969.
RefSeqiNP_416544.1. NC_000913.3.
WP_001023610.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75101; AAC75101; b2040.
BAA15882; BAA15882; BAA15882.
GeneIDi947117.
KEGGiecj:JW2025.
eco:b2040.
PATRICi32119413. VBIEscCol129921_2117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09876 Genomic DNA. Translation: AAB88399.1.
U00096 Genomic DNA. Translation: AAC75101.1.
AP009048 Genomic DNA. Translation: BAA15882.1.
PIRiG64969.
RefSeqiNP_416544.1. NC_000913.3.
WP_001023610.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37760.
SMRiP37760.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261549. 162 interactors.
IntActiP37760. 5 interactors.
STRINGi511145.b2040.

Proteomic databases

PaxDbiP37760.
PRIDEiP37760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75101; AAC75101; b2040.
BAA15882; BAA15882; BAA15882.
GeneIDi947117.
KEGGiecj:JW2025.
eco:b2040.
PATRICi32119413. VBIEscCol129921_2117.

Organism-specific databases

EchoBASEiEB2310.
EcoGeneiEG12411. rfbD.

Phylogenomic databases

eggNOGiENOG4105DBZ. Bacteria.
COG1091. LUCA.
HOGENOMiHOG000227712.
InParanoidiP37760.
KOiK00067.
OMAiGYASRYE.
PhylomeDBiP37760.

Enzyme and pathway databases

UniPathwayiUPA00124.
UPA00281.
BioCyciEcoCyc:DTDPDEHYRHAMREDUCT-MONOMER.
ECOL316407:JW2025-MONOMER.
MetaCyc:DTDPDEHYRHAMREDUCT-MONOMER.

Miscellaneous databases

PROiP37760.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
IPR029903. RmlD-like-bd.
[Graphical view]
PANTHERiPTHR10491. PTHR10491. 1 hit.
PfamiPF04321. RmlD_sub_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01214. rmlD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRMLD_ECOLI
AccessioniPrimary (citable) accession number: P37760
Secondary accession number(s): P76377
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.