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P37760

- RMLD_ECOLI

UniProt

P37760 - RMLD_ECOLI

Protein

dTDP-4-dehydrorhamnose reductase

Gene

rfbD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well By similarity.By similarity

    Catalytic activityi

    dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH.

    Cofactori

    Binds 1 magnesium ion per monomer.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121NAD; via amide nitrogenBy similarity
    Binding sitei102 – 1021NADP; via carbonyl oxygenBy similarity
    Binding sitei153 – 1531Substrate; via amide nitrogenBy similarity
    Binding sitei154 – 1541NAD; via amide nitrogenBy similarity
    Binding sitei223 – 2231SubstrateBy similarity
    Binding sitei260 – 2601SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 115NADBy similarity
    Nucleotide bindingi11 – 122NADPBy similarity
    Nucleotide bindingi30 – 312NADBy similarity
    Nucleotide bindingi39 – 402NAD/NADPBy similarity
    Nucleotide bindingi62 – 654NADBy similarity
    Nucleotide bindingi63 – 653NADPBy similarity
    Nucleotide bindingi128 – 1325NAD/NADPBy similarity

    GO - Molecular functioni

    1. dTDP-4-dehydrorhamnose reductase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
    2. extracellular polysaccharide biosynthetic process Source: UniProtKB
    3. lipopolysaccharide biosynthetic process Source: UniProtKB
    4. O antigen biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipopolysaccharide biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:DTDPDEHYRHAMREDUCT-MONOMER.
    ECOL316407:JW2025-MONOMER.
    MetaCyc:DTDPDEHYRHAMREDUCT-MONOMER.
    UniPathwayiUPA00124.
    UPA00281.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    dTDP-4-dehydrorhamnose reductase (EC:1.1.1.133)
    Alternative name(s):
    dTDP-4-keto-L-rhamnose reductase
    dTDP-6-deoxy-L-lyxo-4-hexulose reductase
    dTDP-6-deoxy-L-mannose dehydrogenase
    dTDP-L-rhamnose synthase
    Gene namesi
    Name:rfbD
    Synonyms:rmlD
    Ordered Locus Names:b2040, JW2025
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12411. rfbD.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 299299dTDP-4-dehydrorhamnose reductasePRO_0000207984Add
    BLAST

    Proteomic databases

    PRIDEiP37760.

    Expressioni

    Gene expression databases

    GenevestigatoriP37760.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiP37760. 5 interactions.
    STRINGi511145.b2040.

    Structurei

    3D structure databases

    ProteinModelPortaliP37760.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni104 – 1052Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000227712.
    KOiK00067.
    OMAiETTWHGY.
    OrthoDBiEOG6HTP2V.
    PhylomeDBiP37760.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR005913. dTDP_dehydrorham_reduct.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF04321. RmlD_sub_bind. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01214. rmlD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P37760-1 [UniParc]FASTAAdd to Basket

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    MNILLFGKTG QVGWELQRAL APLGNLIAFD VHSTDYCGDF SNPEGVAETV    50
    RSIRPDIIVN AAAHTAVDKA ESEPEFAQLI NATSVEAIAK AANEVGAWVI 100
    HYSTDYVFPG NGDMPWLETD ATAPLNVYGE TKLAGEKALQ EYCAKHLIFR 150
    TSWVYAGKGN NFAKTMLRLA KEREELAVIN DQFGAPTGAE LLADCTAHAI 200
    RVALNKPDVA GLYHLVASGT TTWYDYAALV FEEARKAGIP LALNKLNAVP 250
    TTAYPTPARR PHNSRLNTEK FQQNFALVLP DWQVGVKRML NELFTTTAI 299
    Length:299
    Mass (Da):32,694
    Last modified:November 1, 1997 - v2
    Checksum:i1D7C992FA5017AD1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09876 Genomic DNA. Translation: AAB88399.1.
    U00096 Genomic DNA. Translation: AAC75101.1.
    AP009048 Genomic DNA. Translation: BAA15882.1.
    PIRiG64969.
    RefSeqiNP_416544.1. NC_000913.3.
    YP_490282.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75101; AAC75101; b2040.
    BAA15882; BAA15882; BAA15882.
    GeneIDi12932555.
    947117.
    KEGGiecj:Y75_p2003.
    eco:b2040.
    PATRICi32119413. VBIEscCol129921_2117.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09876 Genomic DNA. Translation: AAB88399.1 .
    U00096 Genomic DNA. Translation: AAC75101.1 .
    AP009048 Genomic DNA. Translation: BAA15882.1 .
    PIRi G64969.
    RefSeqi NP_416544.1. NC_000913.3.
    YP_490282.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P37760.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P37760. 5 interactions.
    STRINGi 511145.b2040.

    Proteomic databases

    PRIDEi P37760.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75101 ; AAC75101 ; b2040 .
    BAA15882 ; BAA15882 ; BAA15882 .
    GeneIDi 12932555.
    947117.
    KEGGi ecj:Y75_p2003.
    eco:b2040.
    PATRICi 32119413. VBIEscCol129921_2117.

    Organism-specific databases

    EchoBASEi EB2310.
    EcoGenei EG12411. rfbD.

    Phylogenomic databases

    HOGENOMi HOG000227712.
    KOi K00067.
    OMAi ETTWHGY.
    OrthoDBi EOG6HTP2V.
    PhylomeDBi P37760.

    Enzyme and pathway databases

    UniPathwayi UPA00124 .
    UPA00281 .
    BioCyci EcoCyc:DTDPDEHYRHAMREDUCT-MONOMER.
    ECOL316407:JW2025-MONOMER.
    MetaCyc:DTDPDEHYRHAMREDUCT-MONOMER.

    Miscellaneous databases

    PROi P37760.

    Gene expression databases

    Genevestigatori P37760.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR005913. dTDP_dehydrorham_reduct.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF04321. RmlD_sub_bind. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01214. rmlD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster."
      Stevenson G., Neal B., Liu D., Hobbs M., Packer N.H., Batley M., Redmond J.W., Lindquist L., Reeves P.R.
      J. Bacteriol. 176:4144-4156(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / WG1.
    2. Stevenson G.
      Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 227.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiRMLD_ECOLI
    AccessioniPrimary (citable) accession number: P37760
    Secondary accession number(s): P76377
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3