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Protein

Nitrate/nitrite transporter NarU

Gene

narU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes nitrate uptake, nitrite uptake and nitrite export across the cytoplasmic membrane. May function as a nitrate/H+ and nitrite/H+ channel. Could confer a selective advantage during severe nutrient starvation or slow growth.4 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Nitrate assimilation, Transport

Enzyme and pathway databases

BioCyciEcoCyc:NARU-MONOMER.
ECOL316407:JW1464-MONOMER.
MetaCyc:NARU-MONOMER.

Protein family/group databases

TCDBi2.A.1.8.10. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate/nitrite transporter NarU
Alternative name(s):
Nitrite extrusion protein 2
Nitrite facilitator 2
Gene namesi
Name:narU
Synonyms:yddF
Ordered Locus Names:b1469, JW1464
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12153. narU.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 35CytoplasmicSequence analysisAdd BLAST35
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Topological domaini57 – 76PeriplasmicSequence analysisAdd BLAST20
Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
Topological domaini98 – 101CytoplasmicSequence analysis4
Transmembranei102 – 122HelicalSequence analysisAdd BLAST21
Topological domaini123 – 125PeriplasmicSequence analysis3
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Topological domaini147 – 180CytoplasmicSequence analysisAdd BLAST34
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 206PeriplasmicSequence analysis5
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Topological domaini228 – 258CytoplasmicSequence analysisAdd BLAST31
Transmembranei259 – 279HelicalSequence analysisAdd BLAST21
Topological domaini280 – 287PeriplasmicSequence analysis8
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21
Topological domaini309 – 317CytoplasmicSequence analysis9
Transmembranei318 – 338HelicalSequence analysisAdd BLAST21
Topological domaini339 – 344PeriplasmicSequence analysis6
Transmembranei345 – 365HelicalSequence analysisAdd BLAST21
Topological domaini366 – 401CytoplasmicSequence analysisAdd BLAST36
Transmembranei402 – 422HelicalSequence analysisAdd BLAST21
Topological domaini423 – 432PeriplasmicSequence analysis10
Transmembranei433 – 453HelicalSequence analysisAdd BLAST21
Topological domaini454 – 462CytoplasmicSequence analysis9

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi87R → F, H, K, L, N, P or Q: Loss of activity. 2 Publications1
Mutagenesisi99G → A: No change in activity. 1 Publication1
Mutagenesisi99G → T: Decrease in activity. 1 Publication1
Mutagenesisi113P → A: No change in activity. 1 Publication1
Mutagenesisi113P → C or L: Decrease in activity. 1 Publication1
Mutagenesisi139G → E or I: Loss of activity. 1 Publication1
Mutagenesisi145F → E or W: Loss of activity. 1 Publication1
Mutagenesisi162G → A or S: Loss of activity. 1 Publication1
Mutagenesisi172G → A: No change in activity. 1 Publication1
Mutagenesisi172G → V: Loss of activity. 1 Publication1
Mutagenesisi175G → A or S: Loss of activity. 1 Publication1
Mutagenesisi261Y → N: No change in activity. 1 Publication1
Mutagenesisi266G → A, P or T: Loss of activity. 1 Publication1
Mutagenesisi303R → C, D, K, L, N, P or Q: Loss of activity. 2 Publications1
Mutagenesisi307G → L: Loss of activity. 1 Publication1
Mutagenesisi311D → G or K: Loss of activity. 1 Publication1
Mutagenesisi405G → A, L or V: Loss of activity. 1 Publication1
Mutagenesisi414G → L: No change in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000967291 – 462Nitrate/nitrite transporter NarUAdd BLAST462

Proteomic databases

PaxDbiP37758.
PRIDEiP37758.

Expressioni

Inductioni

Expressed preferentially during the stationary phase in the absence of nitrate.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260180. 13 interactors.
STRINGi511145.b1469.

Structurei

Secondary structure

1462
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 27Combined sources7
Helixi29 – 50Combined sources22
Helixi52 – 55Combined sources4
Helixi57 – 60Combined sources4
Turni61 – 64Combined sources4
Helixi69 – 77Combined sources9
Helixi79 – 83Combined sources5
Turni84 – 86Combined sources3
Helixi87 – 108Combined sources22
Helixi111 – 120Combined sources10
Helixi128 – 139Combined sources12
Helixi140 – 142Combined sources3
Helixi144 – 153Combined sources10
Turni158 – 160Combined sources3
Helixi161 – 172Combined sources12
Helixi174 – 186Combined sources13
Helixi193 – 195Combined sources3
Beta strandi200 – 202Combined sources3
Turni203 – 205Combined sources3
Beta strandi206 – 208Combined sources3
Helixi212 – 215Combined sources4
Helixi217 – 231Combined sources15
Helixi245 – 250Combined sources6
Helixi255 – 258Combined sources4
Turni259 – 265Combined sources7
Helixi266 – 281Combined sources16
Helixi288 – 290Combined sources3
Turni291 – 293Combined sources3
Helixi294 – 309Combined sources16
Turni310 – 312Combined sources3
Helixi315 – 332Combined sources18
Helixi333 – 335Combined sources3
Beta strandi337 – 341Combined sources5
Helixi345 – 374Combined sources30
Helixi396 – 409Combined sources14
Turni410 – 413Combined sources4
Helixi414 – 428Combined sources15
Beta strandi429 – 432Combined sources4
Helixi433 – 452Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IU8X-ray3.11A/B1-462[»]
4IU9X-ray3.00A/B1-462[»]
ProteinModelPortaliP37758.
SMRiP37758.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107QND. Bacteria.
COG2223. LUCA.
HOGENOMiHOG000241722.
InParanoidiP37758.
KOiK02575.
OMAiCIWMVFS.
PhylomeDBiP37758.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004737. NO3_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00886. 2A0108. 1 hit.

Sequencei

Sequence statusi: Complete.

P37758-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALQNEKNSR YLLRDWKPEN PAFWENKGKH IARRNLWISV SCLLLAFCVW
60 70 80 90 100
MLFSAVTVNL NKIGFNFTTD QLFLLTALPS VSGALLRVPY SFMVPIFGGR
110 120 130 140 150
RWTVFSTAIL IIPCVWLGIA VQNPNTPFGI FIVIALLCGF AGANFASSMG
160 170 180 190 200
NISFFFPKAK QGSALGINGG LGNLGVSVMQ LVAPLVIFVP VFAFLGVNGV
210 220 230 240 250
PQADGSVMSL ANAAWIWVPL LAIATIAAWS GMNDIASSRA SIADQLPVLQ
260 270 280 290 300
RLHLWLLSLL YLATFGSFIG FSAGFAMLAK TQFPDVNILR LAFFGPFIGA
310 320 330 340 350
IARSVGGAIS DKFGGVRVTL INFIFMAIFS ALLFLTLPGT GSGNFIAFYA
360 370 380 390 400
VFMGLFLTAG LGSGSTFQMI AVIFRQITIY RVKMKGGSDE QAHKEAVTET
410 420 430 440 450
AAALGFISAI GAVGGFFIPQ AFGMSLNMTG SPVGAMKVFL IFYIVCVLLT
460
WLVYGRRKFS QK
Length:462
Mass (Da):49,890
Last modified:November 1, 1997 - v3
Checksum:i33FBE3C140FBC4DF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti290Missing (Ref. 1) Curated1
Sequence conflicti293F → FR (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94992 Genomic DNA. Translation: CAA64448.1.
U00096 Genomic DNA. Translation: AAD13433.1.
AP009048 Genomic DNA. Translation: BAA15118.1.
X17110 Genomic DNA. No translation available.
PIRiH64899. S11431.
RefSeqiNP_415986.1. NC_000913.3.
WP_001207901.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAD13433; AAD13433; b1469.
BAA15118; BAA15118; BAA15118.
GeneIDi945799.
KEGGiecj:JW1464.
eco:b1469.
PATRICi32118230. VBIEscCol129921_1535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94992 Genomic DNA. Translation: CAA64448.1.
U00096 Genomic DNA. Translation: AAD13433.1.
AP009048 Genomic DNA. Translation: BAA15118.1.
X17110 Genomic DNA. No translation available.
PIRiH64899. S11431.
RefSeqiNP_415986.1. NC_000913.3.
WP_001207901.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IU8X-ray3.11A/B1-462[»]
4IU9X-ray3.00A/B1-462[»]
ProteinModelPortaliP37758.
SMRiP37758.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260180. 13 interactors.
STRINGi511145.b1469.

Protein family/group databases

TCDBi2.A.1.8.10. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP37758.
PRIDEiP37758.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD13433; AAD13433; b1469.
BAA15118; BAA15118; BAA15118.
GeneIDi945799.
KEGGiecj:JW1464.
eco:b1469.
PATRICi32118230. VBIEscCol129921_1535.

Organism-specific databases

EchoBASEiEB2073.
EcoGeneiEG12153. narU.

Phylogenomic databases

eggNOGiENOG4107QND. Bacteria.
COG2223. LUCA.
HOGENOMiHOG000241722.
InParanoidiP37758.
KOiK02575.
OMAiCIWMVFS.
PhylomeDBiP37758.

Enzyme and pathway databases

BioCyciEcoCyc:NARU-MONOMER.
ECOL316407:JW1464-MONOMER.
MetaCyc:NARU-MONOMER.

Miscellaneous databases

PROiP37758.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004737. NO3_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00886. 2A0108. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNARU_ECOLI
AccessioniPrimary (citable) accession number: P37758
Secondary accession number(s): P77696
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.