P37753 (MANC9_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannose-1-phosphate guanylyltransferase
EC=2.7.7.13

Alternative name(s):
GDP-mannose pyrophosphorylase
Short name=GMP
Short name=GMPP

Gene names

Name:	manC
Synonyms:	rfbM, rfbM1

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O9 antigen.
Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1. Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
Miscellaneous	There are two duplicated genes for manB and manC in this E.coli strain.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Mannose-1-phosphate guanylyltransferase		PRO_0000194259
Natural variations
	Natural variant	161	1	V → L.

Sequences

	Sequence		Length	Mass (Da)
	P37753 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: F3A8394550384F79	FASTA	471	52,628
	10 20 30 40 50 60 MLLPVIMAGG TGSRLWPMSR ELYPKQFLRL FGQNSMLQET ITRLSGLEVH EPMVICNEEH 70 80 90 100 110 120 RFLVAEQLRQ LNKLSSNIIL EPVGRNTAPA IALAALQATR HGDDPLMLVL AADHIINNQP 130 140 150 160 170 180 VFHDAIRVAE QYADEGHLVT FGIVPNAPET GYGYIQRGVA VTDSAHTPYQ VARFVEKPDR 190 200 210 220 230 240 ERAGAYLASG EYYWNSGMFM FRAKKYLSEL AKFRPDILEA CQAAVNAADN GSDFISIPHD 250 260 270 280 290 300 IFCECPDESV DYAVMEKTAD AVVVGLDADW SDVGSWSALW EVSPKDGQGN VLSGDAWVHN 310 320 330 340 350 360 SENCYINSDE KLVAAIGVEN LVIVSTKDAV LVMNRERSQD VKKAVEFLKQ NQRTEYKRHR 370 380 390 400 410 420 EIYRPWGRCD VVVQTPRFNV NRITVKPGGA FSMQMHHHRA EHWVILAGTG QVTVNGKQFL 430 440 450 460 470 LSENQSTFIP IGAEHCLENP GCIPLEVLEI QSGSYLGEDD IIRIKDQYGR C

References

[1]

"Cloning and analysis of duplicated rfbM and rfbK genes involved in the formation of GDP-mannose in Escherichia coli O9:K30 and participation of rfb genes in the synthesis of the group I K30 capsular polysaccharide." Jayaratne P., Bronner D., Maclachlan R.P., Dodgson C., Kido N., Whitfield C. J. Bacteriol. 176:3126-3139(1994) [PubMed: 7515042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: O9:K30:H12 / E69.

[2]

"Bacterial polysaccharide synthesis and gene nomenclature." Reeves P.R., Hobbs M., Valvano M.A., Skurnik M., Whitfield C., Coplin D., Kido N., Klena J., Maskell D., Raetz C.R.H., Rick P.D. Trends Microbiol. 4:495-503(1996) [PubMed: 9004408] [Abstract] Cited for: GENE NAME.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L27632 Genomic DNA. Translation: AAA21139.1. L27646 Genomic DNA. Translation: AAA21137.1.
PIR	I41251. I84556.
3D structure databases
ProteinModelPortal	P37753.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MANC9_ECOLX

Accession

Primary (citable) accession number: P37753
Secondary accession number(s): P82273

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	September 21, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program