P37750 (WBBJ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative lipopolysaccharide biosynthesis O-acetyl transferase WbbJ
EC=2.3.1.-

Gene names

Name:	wbbJ
Synonyms:	yefH
Ordered Locus Names:	b2033, JW2018

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Putative O-acetyltransferase that transfers an O-acetyl group to the O antigen.
Pathway	Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	transferase activity, transferring acyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Putative lipopolysaccharide biosynthesis O-acetyl transferase WbbJ		PRO_0000068738
Experimental info
	Sequence conflict	168 – 176	9	SIPENTVIA → LFRKYCHC in AAC31635. Ref.1
	Sequence conflict	187 – 189	3	NHE → IMR in AAC31635. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37750 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: D1C2FA7D3B29A1B1	FASTA	196	21,675
	10 20 30 40 50 60 MILKLAKRYG LCGFIRLVRD VLLTRVFYRN CRIIRFPCYI RNDGSINFGE NFTSGVGLRL 70 80 90 100 110 120 DAFGRGVIFF SDNVQVNDYV HIASIESVTI GRDTLIASKV FITDHNHGSF KHSDPMSSPN 130 140 150 160 170 180 IPPDMRTLES SAVVIGQRVW LGENVTVLPG TIIGNGVVVG ANSVVRGSIP ENTVIAGVPA 190 KIIKKYNHET KLWEKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a." Yao Z., Valvano M.A. J. Bacteriol. 176:4133-4143(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster." Stevenson G., Neal B., Liu D., Hobbs M., Packer N.H., Batley M., Redmond J.W., Lindquist L., Reeves P.R. J. Bacteriol. 176:4144-4156(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / WG1.
[3]	Stevenson G. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 168-176 AND 187-189. Strain: K12 / WG1.
[4]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U03041 Genomic DNA. Translation: AAC31635.1. U09876 Genomic DNA. Translation: AAB88406.1. U00096 Genomic DNA. Translation: AAC75094.1. AP009048 Genomic DNA. Translation: BAA15875.1.
PIR	H64968.
RefSeq	NP_416537.1. NC_000913.2. YP_490275.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P37750.
SMR	P37750. Positions 13-191.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11115N.
IntAct	P37750. 7 interactions.
STRING	511145.b2033.
Proteomic databases
PaxDb	P37750.
PRIDE	P37750.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75094; AAC75094; b2033. BAA15875; BAA15875; BAA15875.
GeneID	12932357. 946556.
KEGG	ecj:Y75_p1996. eco:b2033.
Organism-specific databases
EchoBASE	EB1927.
EcoGene	EG11984. wbbJ.
Phylogenomic databases
eggNOG	COG0110.
HOGENOM	HOG000049435.
KO	K08280.
OMA	AYSEISI.
ProtClustDB	PRK09677.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11984-MONOMER. ECOL316407:JW2018-MONOMER.
UniPathway	UPA00030.
Gene expression databases
Genevestigator	P37750.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. [Graphical view]
Pfam	PF00132. Hexapep. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WBBJ_ECOLI

Accession

Primary (citable) accession number: P37750
Secondary accession number(s): P76375

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program