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Protein

O-antigen polymerase

Gene

rfc

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

May link the O-antigen tetrasaccharide units into long chains, giving rise to typical smooth LPS.

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11982-MONOMER.
ECOL316407:JW2020-MONOMER.
UniPathwayiUPA00030.

Protein family/group databases

TCDBi9.B.67.2.1. the putative inorganic carbon (hco3(-)) transporter/o-antigen polymerase (ict/oap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
O-antigen polymerase
Gene namesi
Name:rfc
Synonyms:yefF
Ordered Locus Names:b2035, JW2020
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11982. rfc.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 4321HelicalSequence analysisAdd
BLAST
Transmembranei57 – 7721HelicalSequence analysisAdd
BLAST
Transmembranei97 – 11721HelicalSequence analysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence analysisAdd
BLAST
Transmembranei180 – 20021HelicalSequence analysisAdd
BLAST
Transmembranei215 – 23521HelicalSequence analysisAdd
BLAST
Transmembranei312 – 33221HelicalSequence analysisAdd
BLAST
Transmembranei338 – 35821HelicalSequence analysisAdd
BLAST
Transmembranei361 – 38121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388O-antigen polymerasePRO_0000097298Add
BLAST

Proteomic databases

PaxDbiP37748.

Interactioni

Protein-protein interaction databases

BioGridi4261358. 165 interactions.
STRINGi511145.b2035.

Structurei

3D structure databases

ProteinModelPortaliP37748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107B0W. Bacteria.
ENOG410Z7XC. LUCA.
KOiK13008.
OMAiLPFVETP.
OrthoDBiEOG6RVG2J.

Sequencei

Sequence statusi: Complete.

P37748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYLVISVFL ITAFICLYLK KDIFYPAVCV NIIFALVLLG YEITSDIYAF
60 70 80 90 100
QLNDATLIFL LCNVLTFTLS CLLTESVLDL NIRKVNNAIY SIPSKKVHNV
110 120 130 140 150
GLLVISFSMI YICMRLSNYQ FGTSLLSYMN LIRDADVEDT SRNFSAYMQP
160 170 180 190 200
IILTTFALFI WSKKFTNTKV SKTFTLLVFI VFIFAIILNT GKQIVFMVII
210 220 230 240 250
SYAFIVGVNR VKHYVYLITA VGVLFSLYML FLRGLPGGMA YYLSMYLVSP
260 270 280 290 300
IIAFQEFYFQ QVSNSASSHV FWFFERLMGL LTGGVSMSLH KEFVWVGLPT
310 320 330 340 350
NVYTAFSDYV YISAELSYLM MVIHGCISGV LWRLSRNYIS VKIFYSYFIY
360 370 380
TFSFIFYHES FMTNISSWIQ ITLCIIVFSQ FLKAQKIK
Length:388
Mass (Da):44,744
Last modified:October 1, 1994 - v1
Checksum:iF6B6A10DBA0199FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03041 Genomic DNA. Translation: AAC31633.1.
U09876 Genomic DNA. Translation: AAB88404.1.
U00096 Genomic DNA. Translation: AAC75096.1.
AP009048 Genomic DNA. Translation: BAA15877.1.
PIRiI69644.
RefSeqiNP_416539.1. NC_000913.3.
WP_000639866.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75096; AAC75096; b2035.
BAA15877; BAA15877; BAA15877.
GeneIDi945179.
KEGGiecj:JW2020.
eco:b2035.
PATRICi32119403. VBIEscCol129921_2111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03041 Genomic DNA. Translation: AAC31633.1.
U09876 Genomic DNA. Translation: AAB88404.1.
U00096 Genomic DNA. Translation: AAC75096.1.
AP009048 Genomic DNA. Translation: BAA15877.1.
PIRiI69644.
RefSeqiNP_416539.1. NC_000913.3.
WP_000639866.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37748.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261358. 165 interactions.
STRINGi511145.b2035.

Protein family/group databases

TCDBi9.B.67.2.1. the putative inorganic carbon (hco3(-)) transporter/o-antigen polymerase (ict/oap) family.

Proteomic databases

PaxDbiP37748.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75096; AAC75096; b2035.
BAA15877; BAA15877; BAA15877.
GeneIDi945179.
KEGGiecj:JW2020.
eco:b2035.
PATRICi32119403. VBIEscCol129921_2111.

Organism-specific databases

EchoBASEiEB1925.
EcoGeneiEG11982. rfc.

Phylogenomic databases

eggNOGiENOG4107B0W. Bacteria.
ENOG410Z7XC. LUCA.
KOiK13008.
OMAiLPFVETP.
OrthoDBiEOG6RVG2J.

Enzyme and pathway databases

UniPathwayiUPA00030.
BioCyciEcoCyc:EG11982-MONOMER.
ECOL316407:JW2020-MONOMER.

Miscellaneous databases

PROiP37748.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a."
    Yao Z., Valvano M.A.
    J. Bacteriol. 176:4133-4143(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster."
    Stevenson G., Neal B., Liu D., Hobbs M., Packer N.H., Batley M., Redmond J.W., Lindquist L., Reeves P.R.
    J. Bacteriol. 176:4144-4156(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / WG1.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiRFC_ECOLI
AccessioniPrimary (citable) accession number: P37748
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 20, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.