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P37747

- GLF_ECOLI

UniProt

P37747 - GLF_ECOLI

Protein

UDP-galactopyranose mutase

Gene

glf

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF).By similarity

    Catalytic activityi

    UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose.1 Publication

    Cofactori

    Binds 1 FAD per subunit.1 Publication

    Kineticsi

    Kcat is 1.5 (sec-1) for UDP-GalF.

    1. KM=194 µM for UDP-GalF1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121FAD; via amide nitrogen1 Publication
    Binding sitei12 – 121UDP-GalP; via amide nitrogenBy similarity
    Binding sitei39 – 391FAD; via amide nitrogen1 Publication
    Binding sitei80 – 801UDP-GalPBy similarity
    Binding sitei152 – 1521UDP-GalPBy similarity
    Binding sitei156 – 1561UDP-GalPBy similarity
    Binding sitei181 – 1811UDP-GalPBy similarity
    Binding sitei268 – 2681UDP-GalPBy similarity
    Binding sitei278 – 2781UDP-GalPBy similarity
    Binding sitei311 – 3111UDP-GalPBy similarity
    Binding sitei340 – 3401FADBy similarity
    Binding sitei346 – 3461UDP-GalPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 322FAD1 Publication
    Nucleotide bindingi56 – 572FAD1 Publication
    Nucleotide bindingi212 – 2132FAD1 Publication
    Nucleotide bindingi347 – 3526FAD1 Publication

    GO - Molecular functioni

    1. UDP-galactopyranose mutase activity Source: EcoCyc

    GO - Biological processi

    1. lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Lipopolysaccharide biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciEcoCyc:GALPMUT-MONOMER.
    ECOL316407:JW2021-MONOMER.
    MetaCyc:GALPMUT-MONOMER.
    UniPathwayiUPA00030.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-galactopyranose mutase (EC:5.4.99.9)
    Short name:
    UGM
    Alternative name(s):
    UDP-GALP mutase
    Uridine 5-diphosphate galactopyranose mutase
    Gene namesi
    Name:glf
    Synonyms:yefE
    Ordered Locus Names:b2036, JW2021
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11981. glf.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi151 – 1511Y → F: 3-fold decrease in the mutase activity. 1 Publication
    Mutagenesisi156 – 1561W → A: Loss of mutase activity. 1 Publication
    Mutagenesisi156 – 1561W → Y: 2-fold decrease in the mutase activity. 1 Publication
    Mutagenesisi181 – 1811Y → F: Increase in the mutase activity. 1 Publication
    Mutagenesisi311 – 3111Y → F: Loss of mutase activity. 1 Publication
    Mutagenesisi346 – 3461Y → F: Loss of mutase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367UDP-galactopyranose mutasePRO_0000087508Add
    BLAST

    Proteomic databases

    PaxDbiP37747.
    PRIDEiP37747.

    Expressioni

    Gene expression databases

    GenevestigatoriP37747.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    DIPiDIP-6863N.
    IntActiP37747. 4 interactions.
    MINTiMINT-1308638.
    STRINGi511145.b2036.

    Structurei

    Secondary structure

    1
    367
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 76
    Helixi11 – 2010
    Helixi21 – 233
    Beta strandi27 – 304
    Beta strandi32 – 376
    Helixi38 – 403
    Beta strandi42 – 454
    Beta strandi48 – 514
    Beta strandi58 – 614
    Helixi63 – 708
    Beta strandi83 – 864
    Beta strandi89 – 968
    Helixi97 – 1048
    Helixi109 – 11911
    Turni120 – 1234
    Helixi131 – 14717
    Helixi149 – 1568
    Helixi160 – 1623
    Beta strandi175 – 1773
    Beta strandi185 – 1884
    Helixi194 – 2029
    Beta strandi205 – 2084
    Helixi213 – 2153
    Helixi217 – 2215
    Beta strandi224 – 2296
    Helixi233 – 2364
    Turni237 – 2415
    Beta strandi246 – 25914
    Beta strandi261 – 2699
    Beta strandi277 – 2815
    Helixi282 – 2854
    Beta strandi293 – 30210
    Helixi316 – 33116
    Beta strandi335 – 3373
    Turni340 – 3445
    Helixi349 – 36416

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1I8TX-ray2.40A/B1-367[»]
    ProteinModelPortaliP37747.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP37747.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0562.
    HOGENOMiHOG000247596.
    KOiK01854.
    OMAiGKQWQTD.
    OrthoDBiEOG62K1X2.
    PhylomeDBiP37747.

    Family and domain databases

    Gene3Di3.40.50.720. 3 hits.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR004379. UDP-GALP_mutase.
    IPR015899. UDP-GalPyranose_mutase_C.
    [Graphical view]
    PANTHERiPTHR21197. PTHR21197. 1 hit.
    PfamiPF03275. GLF. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P37747-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYDYIIVGSG LFGAVCANEL KKLNKKVLVI EKRNHIGGNA YTEDCEGIQI    50
    HKYGAHIFHT NDKYIWDYVN DLVEFNRFTN SPLAIYKDKL FNLPFNMNTF 100
    HQMWGVKDPQ EAQNIINAQK KKYGDKVPEN LEEQAISLVG EDLYQALIKG 150
    YTEKQWGRSA KELPAFIIKR IPVRFTFDNN YFSDRYQGIP VGGYTKLIEK 200
    MLEGVDVKLG IDFLKDKDSL ASKAHRIIYT GPIDQYFDYR FGALEYRSLK 250
    FETERHEFPN FQGNAVINFT DANVPYTRII EHKHFDYVET KHTVVTKEYP 300
    LEWKVGDEPY YPVNDNKNME LFKKYRELAS REDKVIFGGR LAEYKYYDMH 350
    QVISAALYQV KNIMSTD 367
    Length:367
    Mass (Da):42,966
    Last modified:October 1, 1994 - v1
    Checksum:iC62F67A4682B2A06
    GO

    Mass spectrometryi

    Molecular mass is 42960±8 Da from positions 1 - 367. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03041 Genomic DNA. Translation: AAC31632.1.
    U09876 Genomic DNA. Translation: AAB88403.1.
    U00096 Genomic DNA. Translation: AAC75097.1.
    AP009048 Genomic DNA. Translation: BAA15878.1.
    PIRiI69653.
    RefSeqiNP_416540.1. NC_000913.3.
    YP_490278.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75097; AAC75097; b2036.
    BAA15878; BAA15878; BAA15878.
    GeneIDi12931654.
    945235.
    KEGGiecj:Y75_p1999.
    eco:b2036.
    PATRICi32119405. VBIEscCol129921_2112.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03041 Genomic DNA. Translation: AAC31632.1 .
    U09876 Genomic DNA. Translation: AAB88403.1 .
    U00096 Genomic DNA. Translation: AAC75097.1 .
    AP009048 Genomic DNA. Translation: BAA15878.1 .
    PIRi I69653.
    RefSeqi NP_416540.1. NC_000913.3.
    YP_490278.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1I8T X-ray 2.40 A/B 1-367 [» ]
    ProteinModelPortali P37747.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6863N.
    IntActi P37747. 4 interactions.
    MINTi MINT-1308638.
    STRINGi 511145.b2036.

    Chemistry

    ChEMBLi CHEMBL1075076.

    Proteomic databases

    PaxDbi P37747.
    PRIDEi P37747.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75097 ; AAC75097 ; b2036 .
    BAA15878 ; BAA15878 ; BAA15878 .
    GeneIDi 12931654.
    945235.
    KEGGi ecj:Y75_p1999.
    eco:b2036.
    PATRICi 32119405. VBIEscCol129921_2112.

    Organism-specific databases

    EchoBASEi EB1924.
    EcoGenei EG11981. glf.

    Phylogenomic databases

    eggNOGi COG0562.
    HOGENOMi HOG000247596.
    KOi K01854.
    OMAi GKQWQTD.
    OrthoDBi EOG62K1X2.
    PhylomeDBi P37747.

    Enzyme and pathway databases

    UniPathwayi UPA00030 .
    BioCyci EcoCyc:GALPMUT-MONOMER.
    ECOL316407:JW2021-MONOMER.
    MetaCyc:GALPMUT-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P37747.
    PROi P37747.

    Gene expression databases

    Genevestigatori P37747.

    Family and domain databases

    Gene3Di 3.40.50.720. 3 hits.
    InterProi IPR016040. NAD(P)-bd_dom.
    IPR004379. UDP-GALP_mutase.
    IPR015899. UDP-GalPyranose_mutase_C.
    [Graphical view ]
    PANTHERi PTHR21197. PTHR21197. 1 hit.
    Pfami PF03275. GLF. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00031. UDP-GALP_mutase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a."
      Yao Z., Valvano M.A.
      J. Bacteriol. 176:4133-4143(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster."
      Stevenson G., Neal B., Liu D., Hobbs M., Packer N.H., Batley M., Redmond J.W., Lindquist L., Reeves P.R.
      J. Bacteriol. 176:4144-4156(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / WG1.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose mutase."
      Nassau P.M., Martin S.L., Brown R.E., Weston A., Monsey D., McNeil M.R., Duncan K.
      J. Bacteriol. 178:1047-1052(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-15, FUNCTION, CATALYTIC ACTIVITY, MASS SPECTROMETRY, COFACTOR, NOMENCLATURE.
    7. "Mechanistic investigation of UDP-galactopyranose mutase from Escherichia coli using 2- and 3-fluorinated UDP-galactofuranose as probes."
      Zhang Q., Liu H.
      J. Am. Chem. Soc. 123:6756-6766(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    8. Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH FAD, MUTAGENESIS OF TYR-151; TRP-156; TYR-181; TYR-311 AND TYR-346, SUBUNIT.

    Entry informationi

    Entry nameiGLF_ECOLI
    AccessioniPrimary (citable) accession number: P37747
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3