Reviewed, UniProtKB/Swiss-Prot P37747 (GLF_ECOLI)

Names and origin

Protein names

Recommended name:
    UDP-galactopyranose mutase
    EC=5.4.99.9

Gene names

Name:	glf
Synonyms:	yefE
Ordered Locus Names:	b2036, JW2021

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the conversion of UDP-GalP into UDP-GalF through a 2-keto intermediate.
Catalytic activity	UDP-D-galactopyranose = UDP-D-galacto-1,4-furanose.
Cofactor	FAD.
Pathway	Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Mass spectrometry	Molecular mass is 42960±8 Da from positions 1 - 367. Determined by ESI. Ref.6

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	FAD Flavoprotein
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-galactopyranose mutase activity Inferred from electronic annotation. Source: EC oxidoreductase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
tdcD	P11868	1	EBI-558730,EBI-553884

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 367

367

UDP-galactopyranose mutase

PRO_0000087508

Regions

Nucleotide binding

3 – 31

29

FAD Potential

Secondary structure

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367

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Helix

11 – 20

10

Helix

21 – 23

3

Beta strand

27 – 30

4

Beta strand

32 – 37

6

Helix

38 – 40

3

Beta strand

42 – 45

4

Beta strand

48 – 51

4

Beta strand

58 – 61

4

Helix

63 – 70

8

Beta strand

83 – 86

4

Beta strand

89 – 96

8

Helix

97 – 104

8

Helix

109 – 119

11

Turn

120 – 123

4

Helix

131 – 147

17

Helix

149 – 156

8

Helix

160 – 162

3

Beta strand

175 – 177

3

Beta strand

185 – 188

4

Helix

194 – 202

9

Beta strand

205 – 208

4

Helix

213 – 215

3

Helix

217 – 221

5

Beta strand

224 – 229

6

Helix

233 – 236

4

Turn

237 – 241

5

Beta strand

246 – 259

14

Beta strand

261 – 269

9

Beta strand

277 – 281

5

Helix

282 – 285

4

Beta strand

293 – 302

10

Helix

316 – 331

16

Beta strand

335 – 337

3

Turn

340 – 344

5

Helix

349 – 364

16

Sequences

	Sequence		Length	Mass (Da)
	P37747-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: C62F67A4682B2A06	FASTA	367	42,966
	10 20 30 40 50 60 MYDYIIVGSG LFGAVCANEL KKLNKKVLVI EKRNHIGGNA YTEDCEGIQI HKYGAHIFHT 70 80 90 100 110 120 NDKYIWDYVN DLVEFNRFTN SPLAIYKDKL FNLPFNMNTF HQMWGVKDPQ EAQNIINAQK 130 140 150 160 170 180 KKYGDKVPEN LEEQAISLVG EDLYQALIKG YTEKQWGRSA KELPAFIIKR IPVRFTFDNN 190 200 210 220 230 240 YFSDRYQGIP VGGYTKLIEK MLEGVDVKLG IDFLKDKDSL ASKAHRIIYT GPIDQYFDYR 250 260 270 280 290 300 FGALEYRSLK FETERHEFPN FQGNAVINFT DANVPYTRII EHKHFDYVET KHTVVTKEYP 310 320 330 340 350 360 LEWKVGDEPY YPVNDNKNME LFKKYRELAS REDKVIFGGR LAEYKYYDMH QVISAALYQV KNIMSTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a." Yao Z., Valvano M.A. J. Bacteriol. 176:4133-4143(1994) [PubMed: 7517390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster." Stevenson G., Neal B., Liu D., Hobbs M., Packer N.H., Batley M., Redmond J.W., Lindquist L., Reeves P.R. J. Bacteriol. 176:4144-4156(1994) [PubMed: 7517391] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / WG1.
[3]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose mutase." Nassau P.M., Martin S.L., Brown R.E., Weston A., Monsey D., McNeil M.R., Duncan K. J. Bacteriol. 178:1047-1052(1996) [PubMed: 8576037] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15, CHARACTERIZATION, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U03041 Genomic DNA. Translation: AAC31632.1.
U09876 Genomic DNA. Translation: AAB88403.1.
U00096 Genomic DNA. Translation: AAC75097.1.
AP009048 Genomic DNA. Translation: BAA15878.1.

PIR

I69653.

RefSeq

AP_002636.1.
NP_416540.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I8T	X-ray	2.40	A/B	1-367	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:6863N.

IntAct

P37747. 4 interactions.

Genome annotation databases

GeneID

945235.

GenomeReviews

Gene locus JW2021 in contig AP009048_GR.
Gene locus b2036 in contig U00096_GR.

KEGG

ecj:JW2021.
eco:b2036.

Organism-specific databases

EchoBASE

EB1924.

EcoGene

EG11981. glf.

CMR

Search...

Phylogenomic databases

HOGENOM

P37747.

OMA

P37747. KFFRGYT.

Enzyme and pathway databases

BioCyc

EcoCyc:GALPMUT-MON.
MetaCyc:GALPMUT-MON.

Family and domain databases

InterPro

IPR006076. FAD-dep_OxRdtase.
IPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.

Pfam

PF01266. DAO. 1 hit.
PF03275. GLF. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00031. UDP-GALP_mutase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

GLF_ECOLI

Accession

Primary (citable) accession number: P37747

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)