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Reviewed, UniProtKB/Swiss-Prot P37747 (GLF_ECOLI)

Last modified June 16, 2009. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    UDP-galactopyranose mutase
    EC=5.4.99.9
Gene names
Name: glf
Synonyms: yefE
Ordered Locus Names: b2036, JW2021
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Involved in the conversion of UDP-GalP into UDP-GalF through a 2-keto intermediate.

Catalytic activity

UDP-D-galactopyranose = UDP-D-galacto-1,4-furanose.

Cofactor

FAD.

Pathway

Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.

Mass spectrometry

Molecular mass is 42960±8 Da from positions 1 - 367. Determined by ESI. Ref.6

Binary interactions

With

Entry

#Exp.

IntAct

Notes

tdcDP118681EBI-558730,EBI-553884

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367UDP-galactopyranose mutase
PRO_0000087508

Regions

Nucleotide binding3 – 3129FAD Potential

Secondary structure

................................................................... 367
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P37747-1 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: C62F67A4682B2A06

FASTA36742,966
        10         20         30         40         50         60 
MYDYIIVGSG LFGAVCANEL KKLNKKVLVI EKRNHIGGNA YTEDCEGIQI HKYGAHIFHT 

        70         80         90        100        110        120 
NDKYIWDYVN DLVEFNRFTN SPLAIYKDKL FNLPFNMNTF HQMWGVKDPQ EAQNIINAQK 

       130        140        150        160        170        180 
KKYGDKVPEN LEEQAISLVG EDLYQALIKG YTEKQWGRSA KELPAFIIKR IPVRFTFDNN 

       190        200        210        220        230        240 
YFSDRYQGIP VGGYTKLIEK MLEGVDVKLG IDFLKDKDSL ASKAHRIIYT GPIDQYFDYR 

       250        260        270        280        290        300 
FGALEYRSLK FETERHEFPN FQGNAVINFT DANVPYTRII EHKHFDYVET KHTVVTKEYP 

       310        320        330        340        350        360 
LEWKVGDEPY YPVNDNKNME LFKKYRELAS REDKVIFGGR LAEYKYYDMH QVISAALYQV 


KNIMSTD 

« Hide

References

« Hide 'large scale' references
[1]"Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a."
Yao Z., Valvano M.A.
J. Bacteriol. 176:4133-4143(1994) [PubMed: 7517390] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster."
Stevenson G., Neal B., Liu D., Hobbs M., Packer N.H., Batley M., Redmond J.W., Lindquist L., Reeves P.R.
J. Bacteriol. 176:4144-4156(1994) [PubMed: 7517391] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / WG1.
[3]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose mutase."
Nassau P.M., Martin S.L., Brown R.E., Weston A., Monsey D., McNeil M.R., Duncan K.
J. Bacteriol. 178:1047-1052(1996) [PubMed: 8576037] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-15, CHARACTERIZATION, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

U03041 Genomic DNA. Translation: AAC31632.1.
U09876 Genomic DNA. Translation: AAB88403.1.
U00096 Genomic DNA. Translation: AAC75097.1.
AP009048 Genomic DNA. Translation: BAA15878.1.
PIRI69653.
RefSeqAP_002636.1.
NP_416540.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1I8TX-ray2.40A/B1-367[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:6863N.
IntActP37747. 4 interactions.

Genome annotation databases

GeneID945235.
GenomeReviewsGene locus JW2021 in contig AP009048_GR.
Gene locus b2036 in contig U00096_GR.
KEGGecj:JW2021.
eco:b2036.

Organism-specific databases

EchoBASEEB1924.
EcoGeneEG11981. glf.
CMRSearch...

Phylogenomic databases

HOGENOMP37747.
OMAP37747. KFFRGYT.

Enzyme and pathway databases

BioCycEcoCyc:GALPMUT-MON.
MetaCyc:GALPMUT-MON.

Family and domain databases

InterProIPR006076. FAD-dep_OxRdtase.
IPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
PfamPF01266. DAO. 1 hit.
PF03275. GLF. 1 hit.
[Graphical view]
TIGRFAMsTIGR00031. UDP-GALP_mutase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLF_ECOLI
AccessionPrimary (citable) accession number: P37747
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 16, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents