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P37747

- GLF_ECOLI

UniProt

P37747 - GLF_ECOLI

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Protein

UDP-galactopyranose mutase

Gene

glf

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF).By similarity

Catalytic activityi

UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose.1 Publication

Cofactori

Binds 1 FAD per subunit.1 Publication

Kineticsi

Kcat is 1.5 (sec-1) for UDP-GalF.

  1. KM=194 µM for UDP-GalF1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121FAD; via amide nitrogen1 Publication
Binding sitei12 – 121UDP-GalP; via amide nitrogenBy similarity
Binding sitei39 – 391FAD; via amide nitrogen1 Publication
Binding sitei80 – 801UDP-GalPBy similarity
Binding sitei152 – 1521UDP-GalPBy similarity
Binding sitei156 – 1561UDP-GalPBy similarity
Binding sitei181 – 1811UDP-GalPBy similarity
Binding sitei268 – 2681UDP-GalPBy similarity
Binding sitei278 – 2781UDP-GalPBy similarity
Binding sitei311 – 3111UDP-GalPBy similarity
Binding sitei340 – 3401FADBy similarity
Binding sitei346 – 3461UDP-GalPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 322FAD1 Publication
Nucleotide bindingi56 – 572FAD1 Publication
Nucleotide bindingi212 – 2132FAD1 Publication
Nucleotide bindingi347 – 3526FAD1 Publication

GO - Molecular functioni

  1. UDP-galactopyranose mutase activity Source: EcoCyc

GO - Biological processi

  1. lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:GALPMUT-MONOMER.
ECOL316407:JW2021-MONOMER.
MetaCyc:GALPMUT-MONOMER.
UniPathwayiUPA00030.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-galactopyranose mutase (EC:5.4.99.9)
Short name:
UGM
Alternative name(s):
UDP-GALP mutase
Uridine 5-diphosphate galactopyranose mutase
Gene namesi
Name:glf
Synonyms:yefE
Ordered Locus Names:b2036, JW2021
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11981. glf.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi151 – 1511Y → F: 3-fold decrease in the mutase activity. 1 Publication
Mutagenesisi156 – 1561W → A: Loss of mutase activity. 1 Publication
Mutagenesisi156 – 1561W → Y: 2-fold decrease in the mutase activity. 1 Publication
Mutagenesisi181 – 1811Y → F: Increase in the mutase activity. 1 Publication
Mutagenesisi311 – 3111Y → F: Loss of mutase activity. 1 Publication
Mutagenesisi346 – 3461Y → F: Loss of mutase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367UDP-galactopyranose mutasePRO_0000087508Add
BLAST

Proteomic databases

PaxDbiP37747.
PRIDEiP37747.

Expressioni

Gene expression databases

GenevestigatoriP37747.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-6863N.
IntActiP37747. 4 interactions.
MINTiMINT-1308638.
STRINGi511145.b2036.

Structurei

Secondary structure

1
367
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76
Helixi11 – 2010
Helixi21 – 233
Beta strandi27 – 304
Beta strandi32 – 376
Helixi38 – 403
Beta strandi42 – 454
Beta strandi48 – 514
Beta strandi58 – 614
Helixi63 – 708
Beta strandi83 – 864
Beta strandi89 – 968
Helixi97 – 1048
Helixi109 – 11911
Turni120 – 1234
Helixi131 – 14717
Helixi149 – 1568
Helixi160 – 1623
Beta strandi175 – 1773
Beta strandi185 – 1884
Helixi194 – 2029
Beta strandi205 – 2084
Helixi213 – 2153
Helixi217 – 2215
Beta strandi224 – 2296
Helixi233 – 2364
Turni237 – 2415
Beta strandi246 – 25914
Beta strandi261 – 2699
Beta strandi277 – 2815
Helixi282 – 2854
Beta strandi293 – 30210
Helixi316 – 33116
Beta strandi335 – 3373
Turni340 – 3445
Helixi349 – 36416

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I8TX-ray2.40A/B1-367[»]
ProteinModelPortaliP37747.
SMRiP37747. Positions 1-367.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37747.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0562.
HOGENOMiHOG000247596.
InParanoidiP37747.
KOiK01854.
OMAiGKQWQTD.
OrthoDBiEOG62K1X2.
PhylomeDBiP37747.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view]
PANTHERiPTHR21197. PTHR21197. 1 hit.
PfamiPF03275. GLF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.

Sequencei

Sequence statusi: Complete.

P37747-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYDYIIVGSG LFGAVCANEL KKLNKKVLVI EKRNHIGGNA YTEDCEGIQI
60 70 80 90 100
HKYGAHIFHT NDKYIWDYVN DLVEFNRFTN SPLAIYKDKL FNLPFNMNTF
110 120 130 140 150
HQMWGVKDPQ EAQNIINAQK KKYGDKVPEN LEEQAISLVG EDLYQALIKG
160 170 180 190 200
YTEKQWGRSA KELPAFIIKR IPVRFTFDNN YFSDRYQGIP VGGYTKLIEK
210 220 230 240 250
MLEGVDVKLG IDFLKDKDSL ASKAHRIIYT GPIDQYFDYR FGALEYRSLK
260 270 280 290 300
FETERHEFPN FQGNAVINFT DANVPYTRII EHKHFDYVET KHTVVTKEYP
310 320 330 340 350
LEWKVGDEPY YPVNDNKNME LFKKYRELAS REDKVIFGGR LAEYKYYDMH
360
QVISAALYQV KNIMSTD
Length:367
Mass (Da):42,966
Last modified:October 1, 1994 - v1
Checksum:iC62F67A4682B2A06
GO

Mass spectrometryi

Molecular mass is 42960±8 Da from positions 1 - 367. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03041 Genomic DNA. Translation: AAC31632.1.
U09876 Genomic DNA. Translation: AAB88403.1.
U00096 Genomic DNA. Translation: AAC75097.1.
AP009048 Genomic DNA. Translation: BAA15878.1.
PIRiI69653.
RefSeqiNP_416540.1. NC_000913.3.
YP_490278.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75097; AAC75097; b2036.
BAA15878; BAA15878; BAA15878.
GeneIDi12931654.
945235.
KEGGiecj:Y75_p1999.
eco:b2036.
PATRICi32119405. VBIEscCol129921_2112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03041 Genomic DNA. Translation: AAC31632.1 .
U09876 Genomic DNA. Translation: AAB88403.1 .
U00096 Genomic DNA. Translation: AAC75097.1 .
AP009048 Genomic DNA. Translation: BAA15878.1 .
PIRi I69653.
RefSeqi NP_416540.1. NC_000913.3.
YP_490278.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1I8T X-ray 2.40 A/B 1-367 [» ]
ProteinModelPortali P37747.
SMRi P37747. Positions 1-367.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-6863N.
IntActi P37747. 4 interactions.
MINTi MINT-1308638.
STRINGi 511145.b2036.

Chemistry

ChEMBLi CHEMBL1075076.
DrugBanki DB03147. Flavin adenine dinucleotide.

Proteomic databases

PaxDbi P37747.
PRIDEi P37747.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75097 ; AAC75097 ; b2036 .
BAA15878 ; BAA15878 ; BAA15878 .
GeneIDi 12931654.
945235.
KEGGi ecj:Y75_p1999.
eco:b2036.
PATRICi 32119405. VBIEscCol129921_2112.

Organism-specific databases

EchoBASEi EB1924.
EcoGenei EG11981. glf.

Phylogenomic databases

eggNOGi COG0562.
HOGENOMi HOG000247596.
InParanoidi P37747.
KOi K01854.
OMAi GKQWQTD.
OrthoDBi EOG62K1X2.
PhylomeDBi P37747.

Enzyme and pathway databases

UniPathwayi UPA00030 .
BioCyci EcoCyc:GALPMUT-MONOMER.
ECOL316407:JW2021-MONOMER.
MetaCyc:GALPMUT-MONOMER.

Miscellaneous databases

EvolutionaryTracei P37747.
PROi P37747.

Gene expression databases

Genevestigatori P37747.

Family and domain databases

Gene3Di 3.40.50.720. 3 hits.
InterProi IPR016040. NAD(P)-bd_dom.
IPR004379. UDP-GALP_mutase.
IPR015899. UDP-GalPyranose_mutase_C.
[Graphical view ]
PANTHERi PTHR21197. PTHR21197. 1 hit.
Pfami PF03275. GLF. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00031. UDP-GALP_mutase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a."
    Yao Z., Valvano M.A.
    J. Bacteriol. 176:4133-4143(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster."
    Stevenson G., Neal B., Liu D., Hobbs M., Packer N.H., Batley M., Redmond J.W., Lindquist L., Reeves P.R.
    J. Bacteriol. 176:4144-4156(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / WG1.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose mutase."
    Nassau P.M., Martin S.L., Brown R.E., Weston A., Monsey D., McNeil M.R., Duncan K.
    J. Bacteriol. 178:1047-1052(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-15, FUNCTION, CATALYTIC ACTIVITY, MASS SPECTROMETRY, COFACTOR, NOMENCLATURE.
  7. "Mechanistic investigation of UDP-galactopyranose mutase from Escherichia coli using 2- and 3-fluorinated UDP-galactofuranose as probes."
    Zhang Q., Liu H.
    J. Am. Chem. Soc. 123:6756-6766(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  8. Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH FAD, MUTAGENESIS OF TYR-151; TRP-156; TYR-181; TYR-311 AND TYR-346, SUBUNIT.

Entry informationi

Entry nameiGLF_ECOLI
AccessioniPrimary (citable) accession number: P37747
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3