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Protein

UDP-galactopyranose mutase

Gene

glf

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF).By similarity

Catalytic activityi

UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose.1 Publication

Cofactori

FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication

Kineticsi

Kcat is 1.5 (sec-1) for UDP-GalF.

  1. KM=194 µM for UDP-GalF1 Publication

    Pathway:ilipopolysaccharide biosynthesis

    This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.
    View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121FAD; via amide nitrogen1 Publication
    Binding sitei12 – 121UDP-GalP; via amide nitrogenBy similarity
    Binding sitei39 – 391FAD; via amide nitrogen1 Publication
    Binding sitei80 – 801UDP-GalPBy similarity
    Binding sitei152 – 1521UDP-GalPBy similarity
    Binding sitei156 – 1561UDP-GalPBy similarity
    Binding sitei181 – 1811UDP-GalPBy similarity
    Binding sitei268 – 2681UDP-GalPBy similarity
    Binding sitei278 – 2781UDP-GalPBy similarity
    Binding sitei311 – 3111UDP-GalPBy similarity
    Binding sitei340 – 3401FADBy similarity
    Binding sitei346 – 3461UDP-GalPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 322FAD1 Publication
    Nucleotide bindingi56 – 572FAD1 Publication
    Nucleotide bindingi212 – 2132FAD1 Publication
    Nucleotide bindingi347 – 3526FAD1 Publication

    GO - Molecular functioni

    • UDP-galactopyranose mutase activity Source: EcoCyc

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Lipopolysaccharide biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciEcoCyc:GALPMUT-MONOMER.
    ECOL316407:JW2021-MONOMER.
    MetaCyc:GALPMUT-MONOMER.
    BRENDAi5.4.99.9. 2026.
    UniPathwayiUPA00030.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-galactopyranose mutase (EC:5.4.99.9)
    Short name:
    UGM
    Alternative name(s):
    UDP-GALP mutase
    Uridine 5-diphosphate galactopyranose mutase
    Gene namesi
    Name:glf
    Synonyms:yefE
    Ordered Locus Names:b2036, JW2021
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11981. glf.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi151 – 1511Y → F: 3-fold decrease in the mutase activity. 1 Publication
    Mutagenesisi156 – 1561W → A: Loss of mutase activity. 1 Publication
    Mutagenesisi156 – 1561W → Y: 2-fold decrease in the mutase activity. 1 Publication
    Mutagenesisi181 – 1811Y → F: Increase in the mutase activity. 1 Publication
    Mutagenesisi311 – 3111Y → F: Loss of mutase activity. 1 Publication
    Mutagenesisi346 – 3461Y → F: Loss of mutase activity. 1 Publication

    Chemistry

    DrugBankiDB03147. Flavin adenine dinucleotide.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367UDP-galactopyranose mutasePRO_0000087508Add
    BLAST

    Proteomic databases

    PaxDbiP37747.
    PRIDEiP37747.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    DIPiDIP-6863N.
    IntActiP37747. 4 interactions.
    MINTiMINT-1308638.
    STRINGi511145.b2036.

    Structurei

    Secondary structure

    1
    367
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 76Combined sources
    Helixi11 – 2010Combined sources
    Helixi21 – 233Combined sources
    Beta strandi27 – 304Combined sources
    Beta strandi32 – 376Combined sources
    Helixi38 – 403Combined sources
    Beta strandi42 – 454Combined sources
    Beta strandi48 – 514Combined sources
    Beta strandi58 – 614Combined sources
    Helixi63 – 708Combined sources
    Beta strandi83 – 864Combined sources
    Beta strandi89 – 968Combined sources
    Helixi97 – 1048Combined sources
    Helixi109 – 11911Combined sources
    Turni120 – 1234Combined sources
    Helixi131 – 14717Combined sources
    Helixi149 – 1568Combined sources
    Helixi160 – 1623Combined sources
    Beta strandi175 – 1773Combined sources
    Beta strandi185 – 1884Combined sources
    Helixi194 – 2029Combined sources
    Beta strandi205 – 2084Combined sources
    Helixi213 – 2153Combined sources
    Helixi217 – 2215Combined sources
    Beta strandi224 – 2296Combined sources
    Helixi233 – 2364Combined sources
    Turni237 – 2415Combined sources
    Beta strandi246 – 25914Combined sources
    Beta strandi261 – 2699Combined sources
    Beta strandi277 – 2815Combined sources
    Helixi282 – 2854Combined sources
    Beta strandi293 – 30210Combined sources
    Helixi316 – 33116Combined sources
    Beta strandi335 – 3373Combined sources
    Turni340 – 3445Combined sources
    Helixi349 – 36416Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1I8TX-ray2.40A/B1-367[»]
    ProteinModelPortaliP37747.
    SMRiP37747. Positions 1-367.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP37747.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0562.
    HOGENOMiHOG000247596.
    InParanoidiP37747.
    KOiK01854.
    OMAiKYLDMHM.
    OrthoDBiEOG62K1X2.
    PhylomeDBiP37747.

    Family and domain databases

    Gene3Di3.40.50.720. 3 hits.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR004379. UDP-GALP_mutase.
    IPR015899. UDP-GalPyranose_mutase_C.
    [Graphical view]
    PANTHERiPTHR21197. PTHR21197. 1 hit.
    PfamiPF03275. GLF. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P37747-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MYDYIIVGSG LFGAVCANEL KKLNKKVLVI EKRNHIGGNA YTEDCEGIQI
    60 70 80 90 100
    HKYGAHIFHT NDKYIWDYVN DLVEFNRFTN SPLAIYKDKL FNLPFNMNTF
    110 120 130 140 150
    HQMWGVKDPQ EAQNIINAQK KKYGDKVPEN LEEQAISLVG EDLYQALIKG
    160 170 180 190 200
    YTEKQWGRSA KELPAFIIKR IPVRFTFDNN YFSDRYQGIP VGGYTKLIEK
    210 220 230 240 250
    MLEGVDVKLG IDFLKDKDSL ASKAHRIIYT GPIDQYFDYR FGALEYRSLK
    260 270 280 290 300
    FETERHEFPN FQGNAVINFT DANVPYTRII EHKHFDYVET KHTVVTKEYP
    310 320 330 340 350
    LEWKVGDEPY YPVNDNKNME LFKKYRELAS REDKVIFGGR LAEYKYYDMH
    360
    QVISAALYQV KNIMSTD
    Length:367
    Mass (Da):42,966
    Last modified:October 1, 1994 - v1
    Checksum:iC62F67A4682B2A06
    GO

    Mass spectrometryi

    Molecular mass is 42960±8 Da from positions 1 - 367. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U03041 Genomic DNA. Translation: AAC31632.1.
    U09876 Genomic DNA. Translation: AAB88403.1.
    U00096 Genomic DNA. Translation: AAC75097.1.
    AP009048 Genomic DNA. Translation: BAA15878.1.
    PIRiI69653.
    RefSeqiNP_416540.1. NC_000913.3.
    WP_000272486.1. NZ_CP010445.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75097; AAC75097; b2036.
    BAA15878; BAA15878; BAA15878.
    GeneIDi945235.
    KEGGieco:b2036.
    PATRICi32119405. VBIEscCol129921_2112.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U03041 Genomic DNA. Translation: AAC31632.1.
    U09876 Genomic DNA. Translation: AAB88403.1.
    U00096 Genomic DNA. Translation: AAC75097.1.
    AP009048 Genomic DNA. Translation: BAA15878.1.
    PIRiI69653.
    RefSeqiNP_416540.1. NC_000913.3.
    WP_000272486.1. NZ_CP010445.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1I8TX-ray2.40A/B1-367[»]
    ProteinModelPortaliP37747.
    SMRiP37747. Positions 1-367.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-6863N.
    IntActiP37747. 4 interactions.
    MINTiMINT-1308638.
    STRINGi511145.b2036.

    Chemistry

    ChEMBLiCHEMBL1075076.
    DrugBankiDB03147. Flavin adenine dinucleotide.

    Proteomic databases

    PaxDbiP37747.
    PRIDEiP37747.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75097; AAC75097; b2036.
    BAA15878; BAA15878; BAA15878.
    GeneIDi945235.
    KEGGieco:b2036.
    PATRICi32119405. VBIEscCol129921_2112.

    Organism-specific databases

    EchoBASEiEB1924.
    EcoGeneiEG11981. glf.

    Phylogenomic databases

    eggNOGiCOG0562.
    HOGENOMiHOG000247596.
    InParanoidiP37747.
    KOiK01854.
    OMAiKYLDMHM.
    OrthoDBiEOG62K1X2.
    PhylomeDBiP37747.

    Enzyme and pathway databases

    UniPathwayiUPA00030.
    BioCyciEcoCyc:GALPMUT-MONOMER.
    ECOL316407:JW2021-MONOMER.
    MetaCyc:GALPMUT-MONOMER.
    BRENDAi5.4.99.9. 2026.

    Miscellaneous databases

    EvolutionaryTraceiP37747.
    PROiP37747.

    Family and domain databases

    Gene3Di3.40.50.720. 3 hits.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR004379. UDP-GALP_mutase.
    IPR015899. UDP-GalPyranose_mutase_C.
    [Graphical view]
    PANTHERiPTHR21197. PTHR21197. 1 hit.
    PfamiPF03275. GLF. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00031. UDP-GALP_mutase. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a."
      Yao Z., Valvano M.A.
      J. Bacteriol. 176:4133-4143(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster."
      Stevenson G., Neal B., Liu D., Hobbs M., Packer N.H., Batley M., Redmond J.W., Lindquist L., Reeves P.R.
      J. Bacteriol. 176:4144-4156(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / WG1.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose mutase."
      Nassau P.M., Martin S.L., Brown R.E., Weston A., Monsey D., McNeil M.R., Duncan K.
      J. Bacteriol. 178:1047-1052(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-15, FUNCTION, CATALYTIC ACTIVITY, MASS SPECTROMETRY, COFACTOR, NOMENCLATURE.
    7. "Mechanistic investigation of UDP-galactopyranose mutase from Escherichia coli using 2- and 3-fluorinated UDP-galactofuranose as probes."
      Zhang Q., Liu H.
      J. Am. Chem. Soc. 123:6756-6766(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    8. Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH FAD, MUTAGENESIS OF TYR-151; TRP-156; TYR-181; TYR-311 AND TYR-346, SUBUNIT.

    Entry informationi

    Entry nameiGLF_ECOLI
    AccessioniPrimary (citable) accession number: P37747
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: July 22, 2015
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.