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Protein

Putative O-antigen transporter

Gene

rfbX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units.

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoCyc
  • O antigen biosynthetic process Source: UniProtKB-UniPathway
  • response to antibiotic Source: EcoCyc
  • transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Lipopolysaccharide biosynthesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:RFBX-MONOMER.
ECOL316407:JW2022-MONOMER.
MetaCyc:RFBX-MONOMER.
UniPathwayiUPA00281.

Protein family/group databases

TCDBi2.A.66.2.1. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative O-antigen transporter
Gene namesi
Name:rfbX
Synonyms:wzx
Ordered Locus Names:b2037, JW2022
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11980. rfbX.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence analysisAdd
BLAST
Transmembranei12 – 3221HelicalSequence analysisAdd
BLAST
Topological domaini33 – 419PeriplasmicSequence analysis
Transmembranei42 – 6221HelicalSequence analysisAdd
BLAST
Topological domaini63 – 8321CytoplasmicSequence analysisAdd
BLAST
Transmembranei84 – 10421HelicalSequence analysisAdd
BLAST
Topological domaini105 – 11713PeriplasmicSequence analysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence analysisAdd
BLAST
Topological domaini139 – 17335CytoplasmicSequence analysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence analysisAdd
BLAST
Topological domaini195 – 22026PeriplasmicSequence analysisAdd
BLAST
Transmembranei221 – 24121HelicalSequence analysisAdd
BLAST
Topological domaini242 – 29554CytoplasmicSequence analysisAdd
BLAST
Transmembranei296 – 31621HelicalSequence analysisAdd
BLAST
Topological domaini317 – 32812PeriplasmicSequence analysisAdd
BLAST
Transmembranei329 – 34921HelicalSequence analysisAdd
BLAST
Topological domaini350 – 36213CytoplasmicSequence analysisAdd
BLAST
Transmembranei363 – 38321HelicalSequence analysisAdd
BLAST
Topological domaini384 – 3852PeriplasmicSequence analysis
Transmembranei386 – 40621HelicalSequence analysisAdd
BLAST
Topological domaini407 – 4159CytoplasmicSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Putative O-antigen transporterPRO_0000166449Add
BLAST

Proteomic databases

PaxDbiP37746.

Interactioni

Protein-protein interaction databases

BioGridi4259620. 172 interactions.
STRINGi511145.b2037.

Structurei

3D structure databases

ProteinModelPortaliP37746.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polysaccharide synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105ER6. Bacteria.
COG2244. LUCA.
HOGENOMiHOG000176959.
InParanoidiP37746.
KOiK18799.
OMAiIRTQYLI.
PhylomeDBiP37746.

Family and domain databases

InterProiIPR002797. Polysacc_synth.
[Graphical view]
PfamiPF01943. Polysacc_synt. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTNKLSLRR NVIYLAVVQG SNYLLPLLTF PYLVRTLGPE NFGIFGFCQA
60 70 80 90 100
TMLYMIMFVE YGFNLTATQS IAKAADSKDK VTSIFWAVIF SKIVLIVITL
110 120 130 140 150
IFLTSMTLLV PEYNKHAVII WSFVPALVGN LIYPIWLFQG KEKMKWLTLS
160 170 180 190 200
SILSRLAIIP LTFIFVNTKS DIAIAGFIQS SANLVAGIIA LAIVVHEGWI
210 220 230 240 250
GKVTLSLHNV RRSLADGFHV FISTSAISLY STGIVIILGF ISGPTSVGNF
260 270 280 290 300
NAANTIRNAL QGLLNPITQA IYPRISSTLV LNRVKGVILI KKSLTCLSLI
310 320 330 340 350
GGAFSLILLL GASILVKISI GPGYDNAVIV LMIISPLPFL ISLSNVYGIQ
360 370 380 390 400
VMLTHNYKKE FSKILIAAGL LSLLLIFPLT TLFKEIGAAI TLLATECLVT
410
SLMLMFVRNN KLLVC
Length:415
Mass (Da):45,383
Last modified:May 30, 2000 - v4
Checksum:iBE902BE9241CBA5F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti236 – 2361I → V in AAC31631 (PubMed:7517390).Curated
Sequence conflicti350 – 3501Q → R in AAC31631 (PubMed:7517390).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03041 Genomic DNA. Translation: AAC31631.1.
U09876 Genomic DNA. Translation: AAB88402.1.
U00096 Genomic DNA. Translation: AAC75098.1.
AP009048 Genomic DNA. Translation: BAA15879.1.
PIRiD64969.
RefSeqiNP_416541.1. NC_000913.3.
WP_001393538.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75098; AAC75098; b2037.
BAA15879; BAA15879; BAA15879.
GeneIDi946557.
KEGGiecj:JW2022.
eco:b2037.
PATRICi32119407. VBIEscCol129921_2113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03041 Genomic DNA. Translation: AAC31631.1.
U09876 Genomic DNA. Translation: AAB88402.1.
U00096 Genomic DNA. Translation: AAC75098.1.
AP009048 Genomic DNA. Translation: BAA15879.1.
PIRiD64969.
RefSeqiNP_416541.1. NC_000913.3.
WP_001393538.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37746.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259620. 172 interactions.
STRINGi511145.b2037.

Protein family/group databases

TCDBi2.A.66.2.1. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Proteomic databases

PaxDbiP37746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75098; AAC75098; b2037.
BAA15879; BAA15879; BAA15879.
GeneIDi946557.
KEGGiecj:JW2022.
eco:b2037.
PATRICi32119407. VBIEscCol129921_2113.

Organism-specific databases

EchoBASEiEB1923.
EcoGeneiEG11980. rfbX.

Phylogenomic databases

eggNOGiENOG4105ER6. Bacteria.
COG2244. LUCA.
HOGENOMiHOG000176959.
InParanoidiP37746.
KOiK18799.
OMAiIRTQYLI.
PhylomeDBiP37746.

Enzyme and pathway databases

UniPathwayiUPA00281.
BioCyciEcoCyc:RFBX-MONOMER.
ECOL316407:JW2022-MONOMER.
MetaCyc:RFBX-MONOMER.

Miscellaneous databases

PROiP37746.

Family and domain databases

InterProiIPR002797. Polysacc_synth.
[Graphical view]
PfamiPF01943. Polysacc_synt. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFBX_ECOLI
AccessioniPrimary (citable) accession number: P37746
Secondary accession number(s): P76376
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 30, 2000
Last modified: September 7, 2016
This is version 121 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.