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Protein

dTDP-4-dehydrorhamnose 3,5-epimerase

Gene

rfbC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.By similarity

Catalytic activityi

dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose.

Pathwayi: dTDP-L-rhamnose biosynthesis

This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23SubstrateBy similarity1
Binding sitei28SubstrateBy similarity1
Binding sitei47SubstrateBy similarity1
Binding sitei49SubstrateBy similarity1
Binding sitei59SubstrateBy similarity1
Binding sitei62SubstrateBy similarity1
Sitei62Important in abstrating the protons in C3' and C5'By similarity1
Binding sitei72SubstrateBy similarity1
Binding sitei83SubstrateBy similarity1
Binding sitei119SubstrateBy similarity1
Sitei132May have a key role in proton donation to C5'By similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoCyc
  • dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
  • extracellular polysaccharide biosynthetic process Source: UniProtKB
  • lipopolysaccharide biosynthetic process Source: UniProtKB
  • O antigen biosynthetic process Source: UniProtKB-UniPathway
  • response to antibiotic Source: EcoCyc
  • response to UV Source: EcoCyc

Keywordsi

Molecular functionIsomerase
Biological processLipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:DTDPDEHYDRHAMEPIM-MONOMER.
MetaCyc:DTDPDEHYDRHAMEPIM-MONOMER.
UniPathwayiUPA00124.
UPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-4-dehydrorhamnose 3,5-epimerase (EC:5.1.3.13)
Alternative name(s):
Thymidine diphospho-4-keto-rhamnose 3,5-epimerase
dTDP-4-keto-6-deoxyglucose 3,5-epimerase
dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase
dTDP-L-rhamnose synthase
Gene namesi
Name:rfbC
Synonyms:rmlC
Ordered Locus Names:b2038, JW2023
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11979. rfbC.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002079781 – 185dTDP-4-dehydrorhamnose 3,5-epimeraseAdd BLAST185

Proteomic databases

PaxDbiP37745.
PRIDEiP37745.

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F53EBI-557071,EBI-543750

Protein-protein interaction databases

BioGridi4261258. 213 interactors.
DIPiDIP-10681N.
IntActiP37745. 3 interactors.
MINTiMINT-1257696.
STRINGi316385.ECDH10B_2188.

Structurei

3D structure databases

ProteinModelPortaliP37745.
SMRiP37745.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni168 – 169Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108URP. Bacteria.
COG1898. LUCA.
HOGENOMiHOG000227724.
InParanoidiP37745.
KOiK01790.
PhylomeDBiP37745.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiView protein in InterPro
IPR000888. dTDP_sugar_isom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
PANTHERiPTHR21047. PTHR21047. 1 hit.
PfamiView protein in Pfam
PF00908. dTDP_sugar_isom. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001462. dTDP_sugar_isom. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01221. rmlC. 1 hit.

Sequencei

Sequence statusi: Complete.

P37745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVIRTEIED VLILEPRVFG DDRGFFYESF NQSAFEHILG YPVSFVQDNH
60 70 80 90 100
SRSSKNVLRG LHFQRGEYAQ DKLVRCTHGA VFDVAVDIRP NSVSFGKWVG
110 120 130 140 150
VLLSADNKQQ LWIPKGFAHG FLVLSDIAEF QYKTTNYYHP ESDCGICWND
160 170 180
ERIAIDWPQT SGLILSPKDE RLFTLDELIR LKLIA
Length:185
Mass (Da):21,270
Last modified:October 1, 1994 - v1
Checksum:i60C50851A7046BA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03041 Genomic DNA. Translation: AAC31630.1.
U09876 Genomic DNA. Translation: AAB88401.1.
U00096 Genomic DNA. Translation: AAC75099.1.
AP009048 Genomic DNA. Translation: BAA15880.1.
PIRiI69651.
RefSeqiNP_416542.1. NC_000913.3.
WP_001100981.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75099; AAC75099; b2038.
BAA15880; BAA15880; BAA15880.
GeneIDi947482.
KEGGiecj:JW2023.
eco:b2038.
PATRICifig|1411691.4.peg.213.

Similar proteinsi

Entry informationi

Entry nameiRMLC_ECOLI
AccessioniPrimary (citable) accession number: P37745
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 25, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families