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Protein

dTDP-4-dehydrorhamnose 3,5-epimerase

Gene

rfbC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.By similarity

Catalytic activityi

dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose.

Pathwayi: dTDP-L-rhamnose biosynthesis

This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231SubstrateBy similarity
Binding sitei28 – 281SubstrateBy similarity
Binding sitei47 – 471SubstrateBy similarity
Binding sitei49 – 491SubstrateBy similarity
Binding sitei59 – 591SubstrateBy similarity
Binding sitei62 – 621SubstrateBy similarity
Sitei62 – 621Important in abstrating the protons in C3' and C5'By similarity
Binding sitei72 – 721SubstrateBy similarity
Binding sitei83 – 831SubstrateBy similarity
Binding sitei119 – 1191SubstrateBy similarity
Sitei132 – 1321May have a key role in proton donation to C5'By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoCyc
  • dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
  • extracellular polysaccharide biosynthetic process Source: UniProtKB
  • lipopolysaccharide biosynthetic process Source: UniProtKB
  • O antigen biosynthetic process Source: UniProtKB-UniPathway
  • response to antibiotic Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:DTDPDEHYDRHAMEPIM-MONOMER.
ECOL316407:JW2023-MONOMER.
MetaCyc:DTDPDEHYDRHAMEPIM-MONOMER.
UniPathwayiUPA00124.
UPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-4-dehydrorhamnose 3,5-epimerase (EC:5.1.3.13)
Alternative name(s):
Thymidine diphospho-4-keto-rhamnose 3,5-epimerase
dTDP-4-keto-6-deoxyglucose 3,5-epimerase
dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase
dTDP-L-rhamnose synthase
Gene namesi
Name:rfbC
Synonyms:rmlC
Ordered Locus Names:b2038, JW2023
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11979. rfbC.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185dTDP-4-dehydrorhamnose 3,5-epimerasePRO_0000207978Add
BLAST

Proteomic databases

PaxDbiP37745.
PRIDEiP37745.

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F52EBI-557071,EBI-543750

Protein-protein interaction databases

BioGridi4261258. 213 interactions.
DIPiDIP-10681N.
IntActiP37745. 3 interactions.
MINTiMINT-1257696.
STRINGi511145.b2038.

Structurei

3D structure databases

ProteinModelPortaliP37745.
SMRiP37745. Positions 1-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni168 – 1692Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108URP. Bacteria.
COG1898. LUCA.
HOGENOMiHOG000227724.
InParanoidiP37745.
KOiK01790.
OMAiPMEQGKL.
PhylomeDBiP37745.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR000888. dTDP_sugar_isom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR21047. PTHR21047. 1 hit.
PfamiPF00908. dTDP_sugar_isom. 1 hit.
[Graphical view]
ProDomiPD001462. dTDP_sugar_isom. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01221. rmlC. 1 hit.

Sequencei

Sequence statusi: Complete.

P37745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVIRTEIED VLILEPRVFG DDRGFFYESF NQSAFEHILG YPVSFVQDNH
60 70 80 90 100
SRSSKNVLRG LHFQRGEYAQ DKLVRCTHGA VFDVAVDIRP NSVSFGKWVG
110 120 130 140 150
VLLSADNKQQ LWIPKGFAHG FLVLSDIAEF QYKTTNYYHP ESDCGICWND
160 170 180
ERIAIDWPQT SGLILSPKDE RLFTLDELIR LKLIA
Length:185
Mass (Da):21,270
Last modified:October 1, 1994 - v1
Checksum:i60C50851A7046BA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03041 Genomic DNA. Translation: AAC31630.1.
U09876 Genomic DNA. Translation: AAB88401.1.
U00096 Genomic DNA. Translation: AAC75099.1.
AP009048 Genomic DNA. Translation: BAA15880.1.
PIRiI69651.
RefSeqiNP_416542.1. NC_000913.3.
WP_001100981.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75099; AAC75099; b2038.
BAA15880; BAA15880; BAA15880.
GeneIDi947482.
KEGGiecj:JW2023.
eco:b2038.
PATRICi32119409. VBIEscCol129921_2114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03041 Genomic DNA. Translation: AAC31630.1.
U09876 Genomic DNA. Translation: AAB88401.1.
U00096 Genomic DNA. Translation: AAC75099.1.
AP009048 Genomic DNA. Translation: BAA15880.1.
PIRiI69651.
RefSeqiNP_416542.1. NC_000913.3.
WP_001100981.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP37745.
SMRiP37745. Positions 1-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261258. 213 interactions.
DIPiDIP-10681N.
IntActiP37745. 3 interactions.
MINTiMINT-1257696.
STRINGi511145.b2038.

Proteomic databases

PaxDbiP37745.
PRIDEiP37745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75099; AAC75099; b2038.
BAA15880; BAA15880; BAA15880.
GeneIDi947482.
KEGGiecj:JW2023.
eco:b2038.
PATRICi32119409. VBIEscCol129921_2114.

Organism-specific databases

EchoBASEiEB1922.
EcoGeneiEG11979. rfbC.

Phylogenomic databases

eggNOGiENOG4108URP. Bacteria.
COG1898. LUCA.
HOGENOMiHOG000227724.
InParanoidiP37745.
KOiK01790.
OMAiPMEQGKL.
PhylomeDBiP37745.

Enzyme and pathway databases

UniPathwayiUPA00124.
UPA00281.
BioCyciEcoCyc:DTDPDEHYDRHAMEPIM-MONOMER.
ECOL316407:JW2023-MONOMER.
MetaCyc:DTDPDEHYDRHAMEPIM-MONOMER.

Miscellaneous databases

PROiP37745.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR000888. dTDP_sugar_isom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR21047. PTHR21047. 1 hit.
PfamiPF00908. dTDP_sugar_isom. 1 hit.
[Graphical view]
ProDomiPD001462. dTDP_sugar_isom. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01221. rmlC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRMLC_ECOLI
AccessioniPrimary (citable) accession number: P37745
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.