P37741 (MANC7_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannose-1-phosphate guanylyltransferase
EC=2.7.7.13

Alternative name(s):
GDP-mannose pyrophosphorylase
Short name=GMP
Short name=GMPP

Gene names

Name:	manC
Synonyms:	rfbM

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O7 antigen.
Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1. Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Mannose-1-phosphate guanylyltransferase		PRO_0000194258

Sequences

	Sequence		Length	Mass (Da)
	P37741 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: D1133D2D2B22E024	FASTA	464	52,611
	10 20 30 40 50 60 MSSPLIPVIL SGGNGTRLWP LSREEYPKQF LKLTDSISML QSTISRLDSL NTSSPVVICN 70 80 90 100 110 120 ELHRFIVAEQ LRHLNKLDNN IILEPSGRNT APAICIAALI LKMKHPNENP LMLVLPADHS 130 140 150 160 170 180 VKKVKTFCNT IKSAIPFAEA GNLVSFGIKP THPETGYGYI QKGKVLSDSD IYEVSEVRTF 190 200 210 220 230 240 VEKPNLKTAE SFIEKDEYYW NSGMYLFSVE RYLQELSLYR PDIVKVCQET VKNIHYDMDF 250 260 270 280 290 300 IRLDDKIFRN CPQESIDYAV MEKTKDAVVA TMDIGWNDVG AWSSLWELGK KDSSGNVITG 310 320 330 340 350 360 DIVCHETENS YIYTESGLVA TIGIQDLVII HTKDSLLVSR RDSVQNVKNI VQHLDLSGRK 370 380 390 400 410 420 EHKEHREVFK SWGRCDSIDS SEKYHYQVKR ITVNPSENYR CNYIITVRNI GVVVMGIAKL 430 440 450 460 TVAEEIKILK ENESVYIPAG IKHSLKILDN TTCVNRSLDR FLSC

References

[1]

"Identification, expression, and DNA sequence of the GDP-mannose biosynthesis genes encoded by the O7 rfb gene cluster of strain VW187 (Escherichia coli O7:K1)." Marolda C.L., Valvano M.A. J. Bacteriol. 175:148-158(1993) [PubMed: 7677991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: O7:K1 / VW187.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF125322 Genomic DNA. Translation: AAC27538.1.
PIR	C40630.
3D structure databases
ProteinModelPortal	P37741.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MANC7_ECOLX

Accession

Primary (citable) accession number: P37741

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	September 21, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program