P37739 (DCTS_RHOCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C4-dicarboxylate transport sensor protein dctS
EC=2.7.13.3

Gene names

Name:

dctS

Organism

Rhodobacter capsulatus (Rhodopseudomonas capsulata)

Taxonomic identifier

1061 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Member of the two-component regulatory system dctS/dctR involved in the transport of C4-dicarboxylates. DctS functions as a membrane-associated protein kinase that phosphorylates dctR in response to environmental signals.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	C4-dicarboxylate transport sensor protein dctS		PRO_0000074749
Regions
	Topological domain	1 – 26	26	Cytoplasmic Potential
	Transmembrane	27 – 51	25	Helical; Potential
	Topological domain	52 – 252	201	Periplasmic Potential
	Transmembrane	253 – 273	21	Helical; Potential
	Topological domain	274 – 657	384	Cytoplasmic Potential
	Domain	289 – 361	73	PAS
	Domain	365 – 417	53	PAC
	Domain	437 – 652	216	Histidine kinase
	Region	407 – 422	16	Inter-domain linker Potential
Amino acid modifications
	Modified residue	440	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37739 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 139D2CFC7CFFA69B	FASTA	657	70,143
	10 20 30 40 50 60 MRDTTGGPAG AEVWTVPGLL GARKLDLLAL IPLVAIVALM TLVGALLFAV AQSDANRARA 70 80 90 100 110 120 KLATDALWVE QTLRFQMAVD EDVLVRLALD ASAGASQQAL SARARLHLAA NPETLGLRWY 130 140 150 160 170 180 DATGRLIAAV PEGPGPAEAA LVRQLLASGA LPPRPVYGPV RDGRVVLAER VSASGGVVVA 190 200 210 220 230 240 TVSLPMMLER HLPWWIAEQY GVRISDTSGV LAERARRPIA AAAPRHGISF DPPLAGTTLE 250 260 270 280 290 300 IMAYDAPDAF GNAALLAAIG ALSVFAVLAM VVLHRNALRR RMAEDRLRAE MAFRRAMEES 310 320 330 340 350 360 LTVGMRAKDL SGRILYVNGA FCKLVGLAAE DLVGRAQPMP YWAPDFLEET LARQRQLIEG 370 380 390 400 410 420 QPVPQAFETR FRRSDGSEIE VQVFEAPLID AGGRHRGWMG SVIDITQAKQ AARLARAQDE 430 440 450 460 470 480 SLARTGRLVT LGEMASTLAH ELNQPLAAIA SYAAGGLNLF DQPEPNLTML RQAFEKMGAQ 490 500 510 520 530 540 ARRAGLVIRR VQDFVKKRTP QLAALDLSEV LAEALSITAP VAREHRVKLA SLIEGRIPGV 550 560 570 580 590 600 QADRILIEQV LVNLIRNGVE AMAEGPRTGD DLTVRLARAG AAVTIEVMDR GPGISDAVAA 610 620 630 640 650 SLFDPFTSTK SEGMGMGLNI CRSIVEMHHG SLSHGPRAGG GTVFTVTLPV PQEGAPA

References

[1]

"Sequence analysis and interposon mutagenesis of a sensor-kinase (DctS) and response-regulator (DctR) controlling synthesis of the high-affinity C4-dicarboxylate transport system in Rhodobacter capsulatus." Hamblin M.J., Shaw J.G., Kelly D.J. Mol. Gen. Genet. 237:215-224(1993) [PubMed: 8455557] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33303 / B10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64733 Genomic DNA. Translation: CAA45999.1.
PIR	S30288.
3D structure databases
ProteinModelPortal	P37739.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.7.13.3. 5381.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR001610. PAC. IPR000014. PAS. IPR000700. PAS-assoc_C. IPR013767. PAS_fold. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF00989. PAS. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00086. PAC. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF47384. His_kin_homodim. 1 hit.
TIGRFAMs	TIGR00229. Sensory_box. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. PS50113. PAC. 1 hit. PS50112. PAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCTS_RHOCA

Accession

Primary (citable) accession number: P37739

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	September 21, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program