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Protein

Rab proteins geranylgeranyltransferase component A 1

Gene

Chm

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-binding subunit of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B composed of RABGGTA and RABGGTB, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Besides, a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation.5 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
Rab proteins geranylgeranyltransferase component A 1
Alternative name(s):
Choroideremia protein homolog
Rab escort protein 1
Short name:
REP-1
Gene namesi
Name:Chm
Synonyms:Rep1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2340. Chm.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • Rab-protein geranylgeranyltransferase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi279F → A: Abolishes association with RGGT. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000566881 – 650Rab proteins geranylgeranyltransferase component A 1Add BLAST650

Proteomic databases

PaxDbiP37727.
PRIDEiP37727.

PTM databases

iPTMnetiP37727.
PhosphoSitePlusiP37727.

Expressioni

Tissue specificityi

Most abundant in the heart, brain, and spleen. Lower levels seen in the lung, liver, muscle and kidney. Extremely low levels seen in the testis.

Interactioni

Subunit structurei

Monomer. Heterotrimer composed of RABGGTA, RABGGTB and CHM; within this trimer, RABGGTA and RABGGTB form the catalytic component B, while CHM (component A) mediates Rab protein binding. Can associate with the Rab GGTase dimer (RGGT or component B) prior to Rab protein binding; the association is stabilized by geranylgeranyl pyrophosphate (GGpp). The CHM:RGGT:Rab complex is destabilized by GGpp. Interacts with RAB1A, RAB1B, RAB7A and RAB27A and mediates their prenylation.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rab7aP095272EBI-1039231,EBI-916225

GO - Molecular functioni

  • Rab GTPase binding Source: UniProtKB

Protein-protein interaction databases

IntActiP37727. 1 interactor.
STRINGi10116.ENSRNOP00000000174.

Structurei

Secondary structure

1650
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 14Combined sources5
Helixi18 – 29Combined sources12
Beta strandi34 – 37Combined sources4
Beta strandi39 – 43Combined sources5
Helixi45 – 47Combined sources3
Helixi52 – 61Combined sources10
Helixi75 – 78Combined sources4
Beta strandi83 – 88Combined sources6
Beta strandi95 – 102Combined sources8
Helixi214 – 219Combined sources6
Helixi221 – 223Combined sources3
Beta strandi226 – 229Combined sources4
Beta strandi233 – 237Combined sources5
Helixi238 – 246Combined sources9
Helixi248 – 251Combined sources4
Beta strandi254 – 256Combined sources3
Beta strandi259 – 270Combined sources12
Helixi275 – 280Combined sources6
Beta strandi282 – 284Combined sources3
Helixi286 – 300Combined sources15
Helixi301 – 304Combined sources4
Helixi306 – 310Combined sources5
Turni311 – 314Combined sources4
Helixi317 – 321Combined sources5
Beta strandi324 – 326Combined sources3
Helixi328 – 337Combined sources10
Beta strandi343 – 345Combined sources3
Helixi348 – 361Combined sources14
Beta strandi364 – 373Combined sources10
Helixi379 – 390Combined sources12
Beta strandi394 – 398Combined sources5
Beta strandi401 – 407Combined sources7
Turni408 – 410Combined sources3
Beta strandi413 – 418Combined sources6
Beta strandi423 – 425Combined sources3
Beta strandi427 – 432Combined sources6
Helixi433 – 435Combined sources3
Turni438 – 443Combined sources6
Beta strandi447 – 458Combined sources12
Beta strandi470 – 474Combined sources5
Beta strandi478 – 480Combined sources3
Beta strandi484 – 489Combined sources6
Helixi491 – 493Combined sources3
Beta strandi494 – 496Combined sources3
Beta strandi501 – 508Combined sources8
Helixi513 – 524Combined sources12
Beta strandi542 – 553Combined sources12
Helixi559 – 561Combined sources3
Beta strandi562 – 564Combined sources3
Beta strandi569 – 572Combined sources4
Beta strandi577 – 581Combined sources5
Helixi582 – 595Combined sources14
Beta strandi596 – 598Combined sources3
Beta strandi610 – 613Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LTXX-ray2.70R1-650[»]
1VG0X-ray2.20A1-650[»]
1VG9X-ray2.50A/C/E/G1-650[»]
DisProtiDP00458.
ProteinModelPortaliP37727.
SMRiP37727.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37727.

Family & Domainsi

Sequence similaritiesi

Belongs to the Rab GDI family.Curated

Phylogenomic databases

eggNOGiKOG4405. Eukaryota.
COG5044. LUCA.
HOGENOMiHOG000231282.
HOVERGENiHBG004961.
InParanoidiP37727.
PhylomeDBiP37727.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR018203. GDP_dissociation_inhibitor.
IPR001738. Rab_escort.
[Graphical view]
PfamiPF00996. GDI. 2 hits.
[Graphical view]
PIRSFiPIRSF016550. Rab_ger_ger_transf_A_euk. 1 hit.
PRINTSiPR00893. RABESCORT.
PR00891. RABGDIREP.
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

P37727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADNLPSDFD VIVIGTGLPE SIIAAACSRS GQRVLHVDSR SYYGGNWASF
60 70 80 90 100
SFSGLLSWLK EYQENNDVVT ENSMWQEQIL ENEEAIPLSS KDKTIQHVEV
110 120 130 140 150
FCYASQDLHK DVEEAGALQK NHASVTSAQS AEAAEAAETS CLPTAVEPLS
160 170 180 190 200
MGSCEIPAEQ SQCPGPESSP EVNDAEATGK KENSDAKSST EEPSENVPKV
210 220 230 240 250
QDNTETPKKN RITYSQIIKE GRRFNIDLVS QLLYSRGLLI DLLIKSNVSR
260 270 280 290 300
YAEFKNITRI LAFREGTVEQ VPCSRADVFN SKQLTMVEKR MLMKFLTFCV
310 320 330 340 350
EYEEHPDEYR AYEGTTFSEY LKTQKLTPNL QYFVLHSIAM TSETTSCTVD
360 370 380 390 400
GLKATKKFLQ CLGRYGNTPF LFPLYGQGEL PQCFCRMCAV FGGIYCLRHS
410 420 430 440 450
VQCLVVDKES RKCKAVIDQF GQRIISKHFI IEDSYLSENT CSRVQYRQIS
460 470 480 490 500
RAVLITDGSV LKTDADQQVS ILAVPAEEPG SFGVRVIELC SSTMTCMKGT
510 520 530 540 550
YLVHLTCMSS KTAREDLERV VQKLFTPYTE IEAENEQVEK PRLLWALYFN
560 570 580 590 600
MRDSSDISRD CYNDLPSNVY VCSGPDSGLG NDNAVKQAET LFQQICPNED
610 620 630 640 650
FCPAPPNPED IVLDGDSSQQ EVPESSVTPE TNSETPKEST VLGNPEEPSE
Length:650
Mass (Da):72,480
Last modified:October 1, 1994 - v1
Checksum:i5BF42B445E546282
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13722 mRNA. Translation: AAA87626.1.
PIRiA40686.
RefSeqiNP_058763.1. NM_017067.1.
UniGeneiRn.10191.

Genome annotation databases

GeneIDi24942.
KEGGirno:24942.
UCSCiRGD:2340. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13722 mRNA. Translation: AAA87626.1.
PIRiA40686.
RefSeqiNP_058763.1. NM_017067.1.
UniGeneiRn.10191.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LTXX-ray2.70R1-650[»]
1VG0X-ray2.20A1-650[»]
1VG9X-ray2.50A/C/E/G1-650[»]
DisProtiDP00458.
ProteinModelPortaliP37727.
SMRiP37727.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP37727. 1 interactor.
STRINGi10116.ENSRNOP00000000174.

PTM databases

iPTMnetiP37727.
PhosphoSitePlusiP37727.

Proteomic databases

PaxDbiP37727.
PRIDEiP37727.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24942.
KEGGirno:24942.
UCSCiRGD:2340. rat.

Organism-specific databases

CTDi1121.
RGDi2340. Chm.

Phylogenomic databases

eggNOGiKOG4405. Eukaryota.
COG5044. LUCA.
HOGENOMiHOG000231282.
HOVERGENiHBG004961.
InParanoidiP37727.
PhylomeDBiP37727.

Miscellaneous databases

EvolutionaryTraceiP37727.
PROiP37727.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR018203. GDP_dissociation_inhibitor.
IPR001738. Rab_escort.
[Graphical view]
PfamiPF00996. GDI. 2 hits.
[Graphical view]
PIRSFiPIRSF016550. Rab_ger_ger_transf_A_euk. 1 hit.
PRINTSiPR00893. RABESCORT.
PR00891. RABGDIREP.
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiRAE1_RAT
AccessioniPrimary (citable) accession number: P37727
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.