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Protein

Uroporphyrinogen-III C-methyltransferase

Gene

cobA

Organism
Pseudomonas fluorescens
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin.

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Pathway: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cysG), Strain UM270 sctg_0389_0001, whole genome shotgun sequence (RL74_25690), Siroheme synthase (cysG_1), Siroheme synthase (cysG), Siroheme synthase (cysG), Uroporphyrinogen-III C-methyltransferase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathway: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Siroheme synthase (cysG), Strain UM270 sctg_0389_0001, whole genome shotgun sequence (RL74_25690), Siroheme synthase (cysG_1), Siroheme synthase (cysG), Siroheme synthase (cysG), Uroporphyrinogen-III C-methyltransferase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
UPA00262; UER00211.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Gene namesi
Name:cobA
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 247247Uroporphyrinogen-III C-methyltransferasePRO_0000150373Add
BLAST

Proteomic databases

PRIDEiP37725.

Interactioni

Subunit structurei

Monomer.Curated

Structurei

3D structure databases

ProteinModelPortaliP37725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.Curated

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P37725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAKVWLVGA GPGDPELLTL KAVRALHAAD VVLIDDLVNP RVLEHCPDAR
60 70 80 90 100
VITVGKRGGC RSTPQDFIHR LMLAMRAKAN AWCDLRAATP CIFGRGGEEA
110 120 130 140 150
LWLRERGVEV ELVNGITAGL AGATNCDIPL TLRGVSRGVT LVTAHTQDDS
160 170 180 190 200
SLNWRALAEG GTTLVVYMGV AKLEEIRQQL LEGAMAADTP VAMIENASLP
210 220 230 240
QQRECRSTLA HMHTDAQVFA LKSPAILVIG SVAGAGQAAS ITVSASA
Length:247
Mass (Da):26,062
Last modified:October 1, 1994 - v1
Checksum:iE4BE93D60A629D79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115334 Genomic DNA. Translation: AAD45977.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115334 Genomic DNA. Translation: AAD45977.1.

3D structure databases

ProteinModelPortaliP37725.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP37725.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
UPA00262; UER00211.

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of the cobA gene encoding S-adenosyl-L-methionine: uroporhyrinogen III methyltransferase of Pseudomonas fluorescens."
    de Mot R., Schoofs G., Nagy I., Vanderleyden J.
    Gene 150:199-200(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: OE 28.3.

Entry informationi

Entry nameiSUMT_PSEFL
AccessioniPrimary (citable) accession number: P37725
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.