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Protein

Uroporphyrinogen-III C-methyltransferase

Gene

cobA

Organism
Pseudomonas fluorescens
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin.

Catalytic activityi

2 S-adenosyl-L-methionine + uroporphyrinogen III = 2 S-adenosyl-L-homocysteine + precorrin-2.

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.
Proteins known to be involved in this subpathway in this organism are:
  1. Uroporphyrinogen-III C-methyltransferase (cobA)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Sirohydrochlorin ferrochelatase (RL74_25690), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG_1), Siroheme synthase (cysG), Uroporphyrinogen-III C-methyltransferase (cobA), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cobA), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG), Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processCobalamin biosynthesis, Porphyrin biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211
UPA00262; UER00211

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Gene namesi
Name:cobA
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001503731 – 247Uroporphyrinogen-III C-methyltransferaseAdd BLAST247

Proteomic databases

PRIDEiP37725

Interactioni

Subunit structurei

Monomer.Curated

Structurei

3D structure databases

ProteinModelPortaliP37725
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.Curated

Family and domain databases

CDDicd11642 SUMT, 1 hit
Gene3Di3.30.950.10, 1 hit
3.40.1010.10, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR006366 CobA/CysG_C
IPR003043 Uropor_MeTrfase_CS
PfamiView protein in Pfam
PF00590 TP_methylase, 1 hit
SUPFAMiSSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR01469 cobA_cysG_Cterm, 1 hit
PROSITEiView protein in PROSITE
PS00839 SUMT_1, 1 hit
PS00840 SUMT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P37725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAKVWLVGA GPGDPELLTL KAVRALHAAD VVLIDDLVNP RVLEHCPDAR
60 70 80 90 100
VITVGKRGGC RSTPQDFIHR LMLAMRAKAN AWCDLRAATP CIFGRGGEEA
110 120 130 140 150
LWLRERGVEV ELVNGITAGL AGATNCDIPL TLRGVSRGVT LVTAHTQDDS
160 170 180 190 200
SLNWRALAEG GTTLVVYMGV AKLEEIRQQL LEGAMAADTP VAMIENASLP
210 220 230 240
QQRECRSTLA HMHTDAQVFA LKSPAILVIG SVAGAGQAAS ITVSASA
Length:247
Mass (Da):26,062
Last modified:October 1, 1994 - v1
Checksum:iE4BE93D60A629D79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115334 Genomic DNA Translation: AAD45977.1

Similar proteinsi

Entry informationi

Entry nameiSUMT_PSEFL
AccessioniPrimary (citable) accession number: P37725
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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