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Protein

Myrosinase 1

Gene

TGG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones. These toxic degradation products can deter insect herbivores. Seems to function in abscisic acid (ABA) and methyl jasmonate (MeJA) signaling in guard cells. Functionally redundant with TGG2. Hydrolyzes sinigrin and, with lower efficiency, p-nitrophenyl beta-D-glucoside.4 Publications

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=45 µM for sinigrin (at pH 4.5)1 Publication
  2. KM=34 mM for p-nitrophenyl beta-D-glucoside (at pH 4.5)1 Publication
  1. Vmax=2.3 µmol/min/mg enzyme with sinigrin as substrate (at pH 4.5)1 Publication
  2. Vmax=1.2 µmol/min/mg enzyme with p-nitrophenyl beta-D-glucoside as substrate (at pH 4.5)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57SubstrateBy similarity1
Metal bindingi74Zinc; shared with dimeric partnerBy similarity1
Metal bindingi88Zinc; shared with dimeric partnerBy similarity1
Binding sitei159SubstrateBy similarity1
Binding sitei204SubstrateBy similarity1
Binding sitei205AscorbateBy similarity1
Binding sitei278AscorbateBy similarity1
Binding sitei348SubstrateBy similarity1
Active sitei420NucleophilePROSITE-ProRule annotation1
Binding sitei468SubstrateBy similarity1

GO - Molecular functioni

  • beta-glucosidase activity Source: TAIR
  • glucosinolate glucohydrolase activity Source: UniProtKB-EC
  • metal ion binding Source: UniProtKB-KW
  • scopolin beta-glucosidase activity Source: UniProtKB-EC
  • thioglucosidase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • carbohydrate metabolic process Source: InterPro
  • defense response to insect Source: UniProtKB
  • glucosinolate catabolic process Source: TAIR
  • regulation of stomatal movement Source: UniProtKB
  • response to abscisic acid Source: UniProtKB
  • response to insect Source: TAIR
  • response to salt stress Source: GO_Central

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processAbscisic acid signaling pathway, Plant defense
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:AT5G26000-MONOMER
BRENDAi3.2.1.147 399
ReactomeiR-ATH-189085 Digestion of dietary carbohydrate
SABIO-RKiP37702

Protein family/group databases

CAZyiGH1 Glycoside Hydrolase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase 1 (EC:3.2.1.147)
Alternative name(s):
Beta-glucosidase 38 (EC:3.2.1.21)
Short name:
AtBGLU38
Sinigrinase 1
Thioglucosidase 1
Gene namesi
Name:TGG1
Synonyms:BGLU38
Ordered Locus Names:At5g26000
ORF Names:T1N24.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G26000
TAIRilocus:2180597 AT5G26000

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001177320 – 541Myrosinase 1Add BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 449By similarity
Disulfide bondi32 ↔ 445By similarity
Glycosylationi33N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi175N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi224 ↔ 232By similarity
Glycosylationi236N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi356N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi379N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi493N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi512N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP37702
PRIDEiP37702

2D gel databases

SWISS-2DPAGEiP37702

PTM databases

iPTMnetiP37702

Expressioni

Tissue specificityi

Expressed in guard cells, phloem-associated cells and myrosin cells.1 Publication

Gene expression databases

ExpressionAtlasiP37702 baseline and differential
GenevisibleiP37702 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi17944, 6 interactors
IntActiP37702, 1 interactor
STRINGi3702.AT5G26000.1

Structurei

3D structure databases

ProteinModelPortaliP37702
SMRiP37702
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni475 – 476Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626 Eukaryota
COG2723 LUCA
InParanoidiP37702
KOiK01237
OMAiDETKNSY
OrthoDBiEOG093606O7
PhylomeDBiP37702

Family and domain databases

InterProiView protein in InterPro
IPR001360 Glyco_hydro_1
IPR018120 Glyco_hydro_1_AS
IPR033132 Glyco_hydro_1_N_CS
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR10353 PTHR10353, 1 hit
PfamiView protein in Pfam
PF00232 Glyco_hydro_1, 1 hit
PRINTSiPR00131 GLHYDRLASE1
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00572 GLYCOSYL_HYDROL_F1_1, 1 hit
PS00653 GLYCOSYL_HYDROL_F1_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P37702-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLLMLAFVF LLALATCKGD EFVCEENEPF TCNQTKLFNS GNFEKGFIFG
60 70 80 90 100
VASSAYQVEG GRGRGLNVWD SFTHRFPEKG GADLGNGDTT CDSYTLWQKD
110 120 130 140 150
IDVMDELNST GYRFSIAWSR LLPKGKRSRG VNPGAIKYYN GLIDGLVAKN
160 170 180 190 200
MTPFVTLFHW DLPQTLQDEY NGFLNKTIVD DFKDYADLCF ELFGDRVKNW
210 220 230 240 250
ITINQLYTVP TRGYALGTDA PGRCSPKIDV RCPGGNSSTE PYIVAHNQLL
260 270 280 290 300
AHAAAVDVYR TKYKDDQKGM IGPVMITRWF LPFDHSQESK DATERAKIFF
310 320 330 340 350
HGWFMGPLTE GKYPDIMREY VGDRLPEFSE TEAALVKGSY DFLGLNYYVT
360 370 380 390 400
QYAQNNQTIV PSDVHTALMD SRTTLTSKNA TGHAPGPPFN AASYYYPKGI
410 420 430 440 450
YYVMDYFKTT YGDPLIYVTE NGFSTPGDED FEKATADYKR IDYLCSHLCF
460 470 480 490 500
LSKVIKEKNV NVKGYFAWSL GDNYEFCNGF TVRFGLSYVD FANITGDRDL
510 520 530 540
KASGKWFQKF INVTDEDSTN QDLLRSSVSS KNRDRKSLAD A
Length:541
Mass (Da):61,133
Last modified:October 1, 1994 - v1
Checksum:i3736B735DE7A5BD1
GO
Isoform 2 (identifier: P37702-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     457-541: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:456
Mass (Da):51,481
Checksum:iDE7CE563F352A0BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti385P → H in AAL06896 (PubMed:14593172).Curated1
Sequence conflicti426P → A in AAL25596 (PubMed:14593172).Curated1
Sequence conflicti434A → T in BAH20253 (PubMed:19423640).Curated1
Sequence conflicti441I → N in BAH20253 (PubMed:19423640).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti128S → N in strain: cv. Ta-0. 1 Publication1
Natural varianti261T → K in strain: cv. Ba-1. 1 Publication1
Natural varianti264K → Q in strain: cv. Ba-1. 1 Publication1
Natural varianti427G → A in strain: cv. Ag-0, cv. Ba-1, cv. Mh-0, cv. Mr-0 and Tac-0. 1 Publication1
Natural varianti459N → G in strain: cv. Su-0. 1 Publication1
Natural varianti489V → I in strain: cv. No-0, cv. Rsch-0 and cv. Ta-0. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038446457 – 541Missing in isoform 2. CuratedAdd BLAST85

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11454 mRNA Translation: AAC18869.1
X79194 Genomic DNA Translation: CAA55786.1
AF149413 Genomic DNA Translation: AAD40143.1
CP002688 Genomic DNA Translation: AED93511.1
CP002688 Genomic DNA Translation: AED93512.1
AY045681 mRNA Translation: AAK74039.1
AY054237 mRNA Translation: AAL06896.1
AY058182 mRNA Translation: AAL25596.1
AY090382 mRNA Translation: AAL91284.1
AF083677 mRNA Translation: AAN60236.1
AJ831440 Genomic DNA Translation: CAH40799.1
AJ831441 Genomic DNA Translation: CAH40800.1
AJ831442 Genomic DNA Translation: CAH40801.1
AJ831443 Genomic DNA Translation: CAH40802.1
AJ831444 Genomic DNA Translation: CAH40803.1
AJ831445 Genomic DNA Translation: CAH40804.1
AJ831446 Genomic DNA Translation: CAH40805.1
AJ831447 Genomic DNA Translation: CAH40806.1
AJ831448 Genomic DNA Translation: CAH40807.1
AJ831449 Genomic DNA Translation: CAH40808.1
AJ831450 Genomic DNA Translation: CAH40809.1
AJ831451 Genomic DNA Translation: CAH40810.1
AJ831452 Genomic DNA Translation: CAH40811.1
AJ831453 Genomic DNA Translation: CAH40812.1
AJ831454 Genomic DNA Translation: CAH40813.1
AJ831455 Genomic DNA Translation: CAH40814.1
AJ831456 Genomic DNA Translation: CAH40815.1
AJ831457 Genomic DNA Translation: CAH40816.1
AJ831458 Genomic DNA Translation: CAH40817.1
AJ831459 Genomic DNA Translation: CAH40818.1
AJ831460 Genomic DNA Translation: CAH40819.1
AJ831461 Genomic DNA Translation: CAH40820.1
AJ831462 Genomic DNA Translation: CAH40821.1
AJ831463 Genomic DNA Translation: CAH40822.1
AJ831464 Genomic DNA Translation: CAH40823.1
AJ831465 Genomic DNA Translation: CAH40824.1
AJ831466 Genomic DNA Translation: CAH40825.1
AJ831467 Genomic DNA Translation: CAH40826.1
AK317589 mRNA Translation: BAH20253.1
Z18232 mRNA Translation: CAA79143.1
PIRiS56653
RefSeqiNP_197972.2, NM_122501.3 [P37702-2]
NP_851077.1, NM_180746.3 [P37702-1]
UniGeneiAt.23592
At.47944
At.73176

Genome annotation databases

EnsemblPlantsiAT5G26000.1; AT5G26000.1; AT5G26000 [P37702-1]
AT5G26000.2; AT5G26000.2; AT5G26000 [P37702-2]
GeneIDi832669
GrameneiAT5G26000.1; AT5G26000.1; AT5G26000 [P37702-1]
AT5G26000.2; AT5G26000.2; AT5G26000 [P37702-2]
KEGGiath:AT5G26000

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiBGL38_ARATH
AccessioniPrimary (citable) accession number: P37702
Secondary accession number(s): B9DHN6
, Q3E942, Q3V5A7, Q3V5A8, Q3V5A9, Q3V5B1, Q3V5B2, Q3V5B3, Q3V5B4, Q3V5B5, Q3V5B6, Q3V5B7, Q3V5B8, Q3V5B9, Q3V5C0, Q3V5C2, Q3V5C3, Q3V5C4, Q3V5C5, Q3V5C8, Q3V5D1, Q3V5D2, Q8H7H2, Q93Z31, Q940N8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 25, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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