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Protein

Myrosinase 1

Gene

TGG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones. These toxic degradation products can deter insect herbivores. Seems to function in abscisic acid (ABA) and methyl jasmonate (MeJA) signaling in guard cells. Functionally redundant with TGG2. Hydrolyzes sinigrin and, with lower efficiency, p-nitrophenyl beta-D-glucoside.4 Publications

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=45 µM for sinigrin (at pH 4.5)1 Publication
  2. KM=34 mM for p-nitrophenyl beta-D-glucoside (at pH 4.5)1 Publication
  1. Vmax=2.3 µmol/min/mg enzyme with sinigrin as substrate (at pH 4.5)1 Publication
  2. Vmax=1.2 µmol/min/mg enzyme with p-nitrophenyl beta-D-glucoside as substrate (at pH 4.5)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57SubstrateBy similarity1
Metal bindingi74Zinc; shared with dimeric partnerBy similarity1
Metal bindingi88Zinc; shared with dimeric partnerBy similarity1
Binding sitei159SubstrateBy similarity1
Binding sitei204SubstrateBy similarity1
Binding sitei205AscorbateBy similarity1
Binding sitei278AscorbateBy similarity1
Binding sitei348SubstrateBy similarity1
Active sitei420NucleophilePROSITE-ProRule annotation1
Binding sitei468SubstrateBy similarity1

GO - Molecular functioni

  • beta-glucosidase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • scopolin beta-glucosidase activity Source: UniProtKB-EC
  • thioglucosidase activity Source: UniProtKB

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • carbohydrate metabolic process Source: InterPro
  • defense response to insect Source: UniProtKB
  • glucosinolate catabolic process Source: TAIR
  • regulation of stomatal movement Source: UniProtKB
  • response to abscisic acid Source: UniProtKB
  • response to insect Source: TAIR
  • response to salt stress Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Abscisic acid signaling pathway, Plant defense

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:AT5G26000-MONOMER.
BRENDAi3.2.1.147. 399.
SABIO-RKP37702.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase 1 (EC:3.2.1.147)
Alternative name(s):
Beta-glucosidase 38 (EC:3.2.1.21)
Short name:
AtBGLU38
Sinigrinase 1
Thioglucosidase 1
Gene namesi
Name:TGG1
Synonyms:BGLU38
Ordered Locus Names:At5g26000
ORF Names:T1N24.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G26000.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • cytosolic ribosome Source: TAIR
  • peroxisome Source: TAIR
  • plant-type cell wall Source: TAIR
  • thylakoid Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001177320 – 541Myrosinase 1Add BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 449By similarity
Disulfide bondi32 ↔ 445By similarity
Glycosylationi33N-linked (GlcNAc...)Sequence analysis1
Glycosylationi108N-linked (GlcNAc...)Sequence analysis1
Glycosylationi175N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi224 ↔ 232By similarity
Glycosylationi236N-linked (GlcNAc...)Sequence analysis1
Glycosylationi356N-linked (GlcNAc...)Sequence analysis1
Glycosylationi379N-linked (GlcNAc...)Sequence analysis1
Glycosylationi493N-linked (GlcNAc...)Sequence analysis1
Glycosylationi512N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP37702.
PRIDEiP37702.

2D gel databases

SWISS-2DPAGEP37702.

PTM databases

iPTMnetiP37702.

Expressioni

Tissue specificityi

Expressed in guard cells, phloem-associated cells and myrosin cells.1 Publication

Gene expression databases

GenevisibleiP37702. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi17944. 6 interactors.
IntActiP37702. 1 interactor.
STRINGi3702.AT5G26000.1.

Structurei

3D structure databases

ProteinModelPortaliP37702.
SMRiP37702.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni475 – 476Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
InParanoidiP37702.
KOiK01237.
OMAiPDIMREY.
OrthoDBiEOG093606O7.
PhylomeDBiP37702.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P37702-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLLMLAFVF LLALATCKGD EFVCEENEPF TCNQTKLFNS GNFEKGFIFG
60 70 80 90 100
VASSAYQVEG GRGRGLNVWD SFTHRFPEKG GADLGNGDTT CDSYTLWQKD
110 120 130 140 150
IDVMDELNST GYRFSIAWSR LLPKGKRSRG VNPGAIKYYN GLIDGLVAKN
160 170 180 190 200
MTPFVTLFHW DLPQTLQDEY NGFLNKTIVD DFKDYADLCF ELFGDRVKNW
210 220 230 240 250
ITINQLYTVP TRGYALGTDA PGRCSPKIDV RCPGGNSSTE PYIVAHNQLL
260 270 280 290 300
AHAAAVDVYR TKYKDDQKGM IGPVMITRWF LPFDHSQESK DATERAKIFF
310 320 330 340 350
HGWFMGPLTE GKYPDIMREY VGDRLPEFSE TEAALVKGSY DFLGLNYYVT
360 370 380 390 400
QYAQNNQTIV PSDVHTALMD SRTTLTSKNA TGHAPGPPFN AASYYYPKGI
410 420 430 440 450
YYVMDYFKTT YGDPLIYVTE NGFSTPGDED FEKATADYKR IDYLCSHLCF
460 470 480 490 500
LSKVIKEKNV NVKGYFAWSL GDNYEFCNGF TVRFGLSYVD FANITGDRDL
510 520 530 540
KASGKWFQKF INVTDEDSTN QDLLRSSVSS KNRDRKSLAD A
Length:541
Mass (Da):61,133
Last modified:October 1, 1994 - v1
Checksum:i3736B735DE7A5BD1
GO
Isoform 2 (identifier: P37702-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     457-541: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:456
Mass (Da):51,481
Checksum:iDE7CE563F352A0BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti385P → H in AAL06896 (PubMed:14593172).Curated1
Sequence conflicti426P → A in AAL25596 (PubMed:14593172).Curated1
Sequence conflicti434A → T in BAH20253 (PubMed:19423640).Curated1
Sequence conflicti441I → N in BAH20253 (PubMed:19423640).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti128S → N in strain: cv. Ta-0. 1 Publication1
Natural varianti261T → K in strain: cv. Ba-1. 1 Publication1
Natural varianti264K → Q in strain: cv. Ba-1. 1 Publication1
Natural varianti427G → A in strain: cv. Ag-0, cv. Ba-1, cv. Mh-0, cv. Mr-0 and Tac-0. 1 Publication1
Natural varianti459N → G in strain: cv. Su-0. 1 Publication1
Natural varianti489V → I in strain: cv. No-0, cv. Rsch-0 and cv. Ta-0. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038446457 – 541Missing in isoform 2. CuratedAdd BLAST85

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11454 mRNA. Translation: AAC18869.1.
X79194 Genomic DNA. Translation: CAA55786.1.
AF149413 Genomic DNA. Translation: AAD40143.1.
CP002688 Genomic DNA. Translation: AED93511.1.
CP002688 Genomic DNA. Translation: AED93512.1.
AY045681 mRNA. Translation: AAK74039.1.
AY054237 mRNA. Translation: AAL06896.1.
AY058182 mRNA. Translation: AAL25596.1.
AY090382 mRNA. Translation: AAL91284.1.
AF083677 mRNA. Translation: AAN60236.1.
AJ831440 Genomic DNA. Translation: CAH40799.1.
AJ831441 Genomic DNA. Translation: CAH40800.1.
AJ831442 Genomic DNA. Translation: CAH40801.1.
AJ831443 Genomic DNA. Translation: CAH40802.1.
AJ831444 Genomic DNA. Translation: CAH40803.1.
AJ831445 Genomic DNA. Translation: CAH40804.1.
AJ831446 Genomic DNA. Translation: CAH40805.1.
AJ831447 Genomic DNA. Translation: CAH40806.1.
AJ831448 Genomic DNA. Translation: CAH40807.1.
AJ831449 Genomic DNA. Translation: CAH40808.1.
AJ831450 Genomic DNA. Translation: CAH40809.1.
AJ831451 Genomic DNA. Translation: CAH40810.1.
AJ831452 Genomic DNA. Translation: CAH40811.1.
AJ831453 Genomic DNA. Translation: CAH40812.1.
AJ831454 Genomic DNA. Translation: CAH40813.1.
AJ831455 Genomic DNA. Translation: CAH40814.1.
AJ831456 Genomic DNA. Translation: CAH40815.1.
AJ831457 Genomic DNA. Translation: CAH40816.1.
AJ831458 Genomic DNA. Translation: CAH40817.1.
AJ831459 Genomic DNA. Translation: CAH40818.1.
AJ831460 Genomic DNA. Translation: CAH40819.1.
AJ831461 Genomic DNA. Translation: CAH40820.1.
AJ831462 Genomic DNA. Translation: CAH40821.1.
AJ831463 Genomic DNA. Translation: CAH40822.1.
AJ831464 Genomic DNA. Translation: CAH40823.1.
AJ831465 Genomic DNA. Translation: CAH40824.1.
AJ831466 Genomic DNA. Translation: CAH40825.1.
AJ831467 Genomic DNA. Translation: CAH40826.1.
AK317589 mRNA. Translation: BAH20253.1.
Z18232 mRNA. Translation: CAA79143.1.
PIRiS56653.
RefSeqiNP_197972.2. NM_122501.3. [P37702-2]
NP_851077.1. NM_180746.3. [P37702-1]
UniGeneiAt.23592.
At.47944.
At.73176.

Genome annotation databases

EnsemblPlantsiAT5G26000.1; AT5G26000.1; AT5G26000. [P37702-1]
GeneIDi832669.
GrameneiAT5G26000.1; AT5G26000.1; AT5G26000.
KEGGiath:AT5G26000.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11454 mRNA. Translation: AAC18869.1.
X79194 Genomic DNA. Translation: CAA55786.1.
AF149413 Genomic DNA. Translation: AAD40143.1.
CP002688 Genomic DNA. Translation: AED93511.1.
CP002688 Genomic DNA. Translation: AED93512.1.
AY045681 mRNA. Translation: AAK74039.1.
AY054237 mRNA. Translation: AAL06896.1.
AY058182 mRNA. Translation: AAL25596.1.
AY090382 mRNA. Translation: AAL91284.1.
AF083677 mRNA. Translation: AAN60236.1.
AJ831440 Genomic DNA. Translation: CAH40799.1.
AJ831441 Genomic DNA. Translation: CAH40800.1.
AJ831442 Genomic DNA. Translation: CAH40801.1.
AJ831443 Genomic DNA. Translation: CAH40802.1.
AJ831444 Genomic DNA. Translation: CAH40803.1.
AJ831445 Genomic DNA. Translation: CAH40804.1.
AJ831446 Genomic DNA. Translation: CAH40805.1.
AJ831447 Genomic DNA. Translation: CAH40806.1.
AJ831448 Genomic DNA. Translation: CAH40807.1.
AJ831449 Genomic DNA. Translation: CAH40808.1.
AJ831450 Genomic DNA. Translation: CAH40809.1.
AJ831451 Genomic DNA. Translation: CAH40810.1.
AJ831452 Genomic DNA. Translation: CAH40811.1.
AJ831453 Genomic DNA. Translation: CAH40812.1.
AJ831454 Genomic DNA. Translation: CAH40813.1.
AJ831455 Genomic DNA. Translation: CAH40814.1.
AJ831456 Genomic DNA. Translation: CAH40815.1.
AJ831457 Genomic DNA. Translation: CAH40816.1.
AJ831458 Genomic DNA. Translation: CAH40817.1.
AJ831459 Genomic DNA. Translation: CAH40818.1.
AJ831460 Genomic DNA. Translation: CAH40819.1.
AJ831461 Genomic DNA. Translation: CAH40820.1.
AJ831462 Genomic DNA. Translation: CAH40821.1.
AJ831463 Genomic DNA. Translation: CAH40822.1.
AJ831464 Genomic DNA. Translation: CAH40823.1.
AJ831465 Genomic DNA. Translation: CAH40824.1.
AJ831466 Genomic DNA. Translation: CAH40825.1.
AJ831467 Genomic DNA. Translation: CAH40826.1.
AK317589 mRNA. Translation: BAH20253.1.
Z18232 mRNA. Translation: CAA79143.1.
PIRiS56653.
RefSeqiNP_197972.2. NM_122501.3. [P37702-2]
NP_851077.1. NM_180746.3. [P37702-1]
UniGeneiAt.23592.
At.47944.
At.73176.

3D structure databases

ProteinModelPortaliP37702.
SMRiP37702.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17944. 6 interactors.
IntActiP37702. 1 interactor.
STRINGi3702.AT5G26000.1.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

PTM databases

iPTMnetiP37702.

2D gel databases

SWISS-2DPAGEP37702.

Proteomic databases

PaxDbiP37702.
PRIDEiP37702.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G26000.1; AT5G26000.1; AT5G26000. [P37702-1]
GeneIDi832669.
GrameneiAT5G26000.1; AT5G26000.1; AT5G26000.
KEGGiath:AT5G26000.

Organism-specific databases

TAIRiAT5G26000.

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
InParanoidiP37702.
KOiK01237.
OMAiPDIMREY.
OrthoDBiEOG093606O7.
PhylomeDBiP37702.

Enzyme and pathway databases

BioCyciMetaCyc:AT5G26000-MONOMER.
BRENDAi3.2.1.147. 399.
SABIO-RKP37702.

Miscellaneous databases

PROiP37702.

Gene expression databases

GenevisibleiP37702. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGL38_ARATH
AccessioniPrimary (citable) accession number: P37702
Secondary accession number(s): B9DHN6
, Q3E942, Q3V5A7, Q3V5A8, Q3V5A9, Q3V5B1, Q3V5B2, Q3V5B3, Q3V5B4, Q3V5B5, Q3V5B6, Q3V5B7, Q3V5B8, Q3V5B9, Q3V5C0, Q3V5C2, Q3V5C3, Q3V5C4, Q3V5C5, Q3V5C8, Q3V5D1, Q3V5D2, Q8H7H2, Q93Z31, Q940N8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.