Reviewed,
UniProtKB/Swiss-Prot P37702 (MYRO_ARATH)
Last modified
June 16, 2009.
Version 82.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Myrosinase EC=3.2.1.147 Alternative name(s): Sinigrinase Thioglucosidase | ||||||
| Gene names |
| ||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 541 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones. |
| Catalytic activity | A thioglucoside + H2O = a sugar + a thiol. |
| Cofactor | Binds 1 ascorbate molecule per subunit By similarity. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Vacuole By similarity. |
| Sequence similarities | Belongs to the glycosyl hydrolase 1 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Vacuole |
| Coding sequence diversity | Alternative splicing |
| Domain | Signal |
| Ligand | Metal-binding Zinc |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond Glycoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | glucosinolate catabolic process Ref.2 Inferred from mutant phenotype. Source: TAIR |
| Cellular component | apoplast Inferred from direct assay. Source: TAIR chloroplastInferred from direct assay. Source: TAIR cytosolic ribosomeInferred from direct assay. Source: TAIR peroxisomeInferred from direct assay. Source: TAIR plant-type cell wallInferred from direct assay. Source: TAIR thylakoidInferred from direct assay. Source: TAIR vacuoleInferred from direct assay. Source: TAIR |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct thioglucosidase activityInferred from direct assay. Source: TAIR zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences. | ||||||
| Isoform 1 (identifier: P37702-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | Potential | ||||||||
| Chain | 20 – 541 | 522 | Myrosinase | PRO_0000011773 | |||||||
Regions | |||||||||||
| Region | 204 – 205 | 2 | Substrate By similarity | ||||||||
| Region | 475 – 476 | 2 | Substrate By similarity | ||||||||
Sites | |||||||||||
| Active site | 420 | 1 | Nucleophile By similarity | ||||||||
| Metal binding | 74 | 1 | Zinc; shared with dimeric partner By similarity | ||||||||
| Metal binding | 88 | 1 | Zinc; shared with dimeric partner By similarity | ||||||||
| Binding site | 57 | 1 | Substrate By similarity | ||||||||
| Binding site | 159 | 1 | Substrate By similarity | ||||||||
| Binding site | 205 | 1 | Ascorbate cofactor By similarity | ||||||||
| Binding site | 278 | 1 | Ascorbate cofactor By similarity | ||||||||
| Binding site | 348 | 1 | Substrate By similarity | ||||||||
| Binding site | 389 | 1 | Substrate By similarity | ||||||||
| Binding site | 420 | 1 | Substrate By similarity | ||||||||
| Binding site | 468 | 1 | Substrate By similarity | ||||||||
| Binding site | 484 | 1 | Substrate By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 33 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 108 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 175 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 236 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 356 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 379 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 493 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 512 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 24 ↔ 449 | By similarity | |||||||||
| Disulfide bond | 32 ↔ 445 | By similarity | |||||||||
| Disulfide bond | 224 ↔ 232 | By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 385 | 1 | P → H in AAL06896. Ref.4 | ||||||||
| Sequence conflict | 426 | 1 | P → A in AAL25596. Ref.4 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Arabidopsis cDNA sequence encoding myrosinase." Chadchawan S., Bishop J., Thangstad O.P., Bones A.M., Mitchell-Olds T., Bradley D. Plant Physiol. 103:671-671(1993) [PubMed: 8029343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. |
| [2] | "The myrosinase gene family in Arabidopsis thaliana: gene organization, expression and evolution." Xue J., Joergensen M., Pihlgren U., Rask L. Plant Mol. Biol. 27:911-922(1995) [PubMed: 7766881] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia. Tissue: Leaf. |
| [3] | "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. Fransz P.F.Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [4] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [5] | "Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves and guard cells." Stracke R., Palme K. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-226. |
| [6] | "An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana." Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M., Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D., Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M., Cooke R. Lescure B.Plant J. 4:1051-1061(1993) [PubMed: 8281187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 192-250. Strain: cv. C24. Tissue: Flower bud. |
Cross-references
Sequence databases | |
|---|---|
| L11454 mRNA. Translation: AAC18869.1. X79194 Genomic DNA. Translation: CAA55786.1. AF149413 Genomic DNA. Translation: AAD40143.1. AY045681 mRNA. Translation: AAK74039.1. AY054237 mRNA. Translation: AAL06896.1. AY058182 mRNA. Translation: AAL25596.1. AY090382 mRNA. Translation: AAL91284.1. AF083677 mRNA. Translation: AAN60236.1. Z18232 mRNA. Translation: CAA79143.1. | |
| IPI | IPI00539116. |
| PIR | S56653. |
| RefSeq | NP_851077.1. |
| UniGene | At.23592 At.47944 At.73176 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1E6X based on UniProtKB P29736. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P37702. 1 interaction. |
Protein family/group databases | |
| CAZy | GH1. Glycoside Hydrolase Family 1. |
2-D gel databases | |
| SWISS-2DPAGE | P37702. |
Proteomic databases | |
| PRIDE | P37702. |
Genome annotation databases | |
| GeneID | 832669. |
| GenomeReviews | Gene locus AT5G26000 in contig BA000015_GR. |
| NMPDR | fig|3702.1.peg.24802. |
Organism-specific databases | |
| TAIR | At5g26000. |
Phylogenomic databases | |
| OMA | P37702. AVYITEN. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.147. 302. |
Family and domain databases | |
| InterPro | IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view] |
| Gene3D | G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. |
| PANTHER | PTHR10353. Glyco_hydro_1. 1 hit. |
| Pfam | PF00232. Glyco_hydro_1. 1 hit. [Graphical view] |
| PRINTS | PR00131. GLHYDRLASE1. |
| PROSITE | PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MYRO_ARATH | ||||||||
| Accession | Primary (citable) accession number: P37702 Secondary accession number(s): Q8H7H2, Q93Z31, Q940N8 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

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