ID   GUNG_RUMCH              Reviewed;         725 AA.
AC   P37700; B8I7V3;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 2.
DT   27-MAR-2024, entry version 153.
DE   RecName: Full=Endoglucanase G;
DE            EC=3.2.1.4;
DE   AltName: Full=Cellulase G;
DE   AltName: Full=EGCCG;
DE   AltName: Full=Endo-1,4-beta-glucanase G;
DE   Flags: Precursor;
GN   Name=celCCG; OrderedLocusNames=Ccel_0731;
OS   Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 /
OS   H10) (Clostridium cellulolyticum).
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Ruminiclostridium.
OX   NCBI_TaxID=394503;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1398087; DOI=10.1016/0378-1119(92)90062-t;
RA   Bagnara-Tardif C., Gaudin C., Belaich A., Hoest P., Citard T.,
RA   Belaich J.-P.;
RT   "Sequence analysis of a gene cluster encoding cellulases from Clostridium
RT   cellulolyticum.";
RL   Gene 119:17-28(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35319 / DSM 5812 / JCM 6584 / H10;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Chertkov O., Saunders E., Brettin T.,
RA   Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Ivanova N., Zhou J., Richardson P.;
RT   "Complete sequence of Clostridium cellulolyticum H10.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The biological conversion of cellulose to glucose generally
CC       requires three types of hydrolytic enzymes: (1) Endoglucanases which
CC       cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that
CC       cut the disaccharide cellobiose from the non-reducing end of the
CC       cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the
CC       cellobiose and other short cello-oligosaccharides to glucose.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- PATHWAY: Glycan metabolism; cellulose degradation.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; M87018; AAA73868.1; -; Genomic_DNA.
DR   EMBL; CP001348; ACL75110.1; -; Genomic_DNA.
DR   PIR; JC1300; JC1300.
DR   RefSeq; WP_015924277.1; NC_011898.1.
DR   PDB; 1G87; X-ray; 1.60 A; A/B=36-649.
DR   PDB; 1GA2; X-ray; 1.70 A; A/B=36-649.
DR   PDB; 1K72; X-ray; 1.80 A; A/B=36-649.
DR   PDB; 1KFG; X-ray; 1.90 A; A/B=36-649.
DR   PDBsum; 1G87; -.
DR   PDBsum; 1GA2; -.
DR   PDBsum; 1K72; -.
DR   PDBsum; 1KFG; -.
DR   AlphaFoldDB; P37700; -.
DR   SMR; P37700; -.
DR   STRING; 394503.Ccel_0731; -.
DR   DrugBank; DB03859; 1-thio-beta-D-glucopyranose.
DR   DrugBank; DB03584; 4-Thio-beta-D-glucopyranose.
DR   DrugBank; DB02379; Beta-D-Glucose.
DR   DrugBank; DB02061; Cellobiose.
DR   DrugBank; DB02361; S-Methyl Thiocysteine Group.
DR   CAZy; CBM3; Carbohydrate-Binding Module Family 3.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   KEGG; cce:Ccel_0731; -.
DR   eggNOG; COG4733; Bacteria.
DR   HOGENOM; CLU_008926_0_2_9; -.
DR   OrthoDB; 9758662at2; -.
DR   UniPathway; UPA00696; -.
DR   EvolutionaryTrace; P37700; -.
DR   Proteomes; UP000001349; Chromosome.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030248; F:cellulose binding; IEA:InterPro.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd14256; Dockerin_I; 1.
DR   Gene3D; 1.50.10.10; -; 1.
DR   Gene3D; 1.10.1330.10; Dockerin domain; 1.
DR   Gene3D; 2.60.40.710; Endoglucanase-like; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR   InterPro; IPR001956; CBM3.
DR   InterPro; IPR036966; CBM3_sf.
DR   InterPro; IPR002105; Dockerin_1_rpt.
DR   InterPro; IPR016134; Dockerin_dom.
DR   InterPro; IPR036439; Dockerin_dom_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR   Pfam; PF00942; CBM_3; 1.
DR   Pfam; PF00404; Dockerin_1; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SMART; SM01067; CBM_3; 1.
DR   SUPFAM; SSF49384; Carbohydrate-binding domain; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   SUPFAM; SSF63446; Type I dockerin domain; 1.
DR   PROSITE; PS51172; CBM3; 1.
DR   PROSITE; PS00448; CLOS_CELLULOSOME_RPT; 1.
DR   PROSITE; PS51766; DOCKERIN; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Carbohydrate metabolism; Cellulose degradation; Glycosidase;
KW   Hydrolase; Polysaccharide degradation; Reference proteome; Signal.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..725
FT                   /note="Endoglucanase G"
FT                   /id="PRO_0000007947"
FT   DOMAIN          489..650
FT                   /note="CBM3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00513"
FT   DOMAIN          658..724
FT                   /note="Dockerin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01102"
FT   ACT_SITE        93
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        408
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        446
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        455
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   CONFLICT        609..610
FT                   /note="TT -> RR (in Ref. 1; AAA73868)"
FT                   /evidence="ECO:0000305"
FT   HELIX           41..55
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          65..68
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   TURN            74..77
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           78..80
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          91..93
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           98..122
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           125..141
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          149..154
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           156..160
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           166..168
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          175..178
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          180..182
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           185..202
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   TURN            203..205
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           207..227
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   TURN            235..239
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           246..260
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           263..271
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           272..275
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          282..285
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           297..308
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           311..324
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          345..347
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           348..364
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           370..372
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           373..388
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   TURN            389..391
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          399..402
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           410..413
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          416..418
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          422..425
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           451..454
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           458..475
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          493..504
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          507..516
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          527..535
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           537..541
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           546..548
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          549..552
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          556..558
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          567..570
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           571..573
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          575..581
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          589..591
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   TURN            592..595
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          596..604
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           614..616
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   HELIX           618..620
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          625..628
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          633..635
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          637..639
FT                   /evidence="ECO:0007829|PDB:1G87"
FT   STRAND          642..646
FT                   /evidence="ECO:0007829|PDB:1G87"
SQ   SEQUENCE   725 AA;  79776 MW;  1C87FE4D03797C41 CRC64;
     MLKTKRKLTK AIGVALSISI LSSLVSFIPQ TNTYAAGTYN YGEALQKSIM FYEFQRSGDL
     PADKRDNWRD DSGMKDGSDV GVDLTGGWYD AGDHVKFNLP MSYTSAMLAW SLYEDKDAYD
     KSGQTKYIMD GIKWANDYFI KCNPTPGVYY YQVGDGGKDH SWWGPAEVMQ MERPSFKVDA
     SKPGSAVCAS TAASLASAAV VFKSSDPTYA EKCISHAKNL FDMADKAKSD AGYTAASGYY
     SSSSFYDDLS WAAVWLYLAT NDSTYLDKAE SYVPNWGKEQ QTDIIAYKWG QCWDDVHYGA
     ELLLAKLTNK QLYKDSIEMN LDFWTTGVNG TRVSYTPKGL AWLFQWGSLR HATTQAFLAG
     VYAEWEGCTP SKVSVYKDFL KSQIDYALGS TGRSFVVGYG VNPPQHPHHR TAHGSWTDQM
     TSPTYHRHTI YGALVGGPDN ADGYTDEINN YVNNEIACDY NAGFTGALAK MYKHSGGDPI
     PNFKAIEKIT NDEVIIKAGL NSTGPNYTEI KAVVYNQTGW PARVTDKISF KYFMDLSEIV
     AAGIDPLSLV TSSNYSEGKN TKVSGVLPWD VSNNVYYVNV DLTGENIYPG GQSACRREVQ
     FRIAAPQGTT YWNPKNDFSY DGLPTTSTVN TVTNIPVYDN GVKVFGNEPA GGSENPDPEI
     LYGDVNSDKN VDALDFAALK KYLLGGTSSI DVKAADTYKD GNIDAIDMAT LKKYLLGTIT
     QLPQG
//