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P37700

- GUNG_CLOCE

UniProt

P37700 - GUNG_CLOCE

Protein

Endoglucanase G

Gene

celCCG

Organism
Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (26 May 2009)
      Previous versions | rss
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    Functioni

    The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei408 – 4081By similarity
    Active sitei446 – 4461By similarity
    Active sitei455 – 4551By similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC
    2. cellulose binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCCEL394503:GJET-752-MONOMER.
    UniPathwayiUPA00696.

    Protein family/group databases

    CAZyiCBM3. Carbohydrate-Binding Module Family 3.
    GH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase G (EC:3.2.1.4)
    Alternative name(s):
    Cellulase G
    EGCCG
    Endo-1,4-beta-glucanase G
    Gene namesi
    Name:celCCG
    Ordered Locus Names:Ccel_0731
    OrganismiClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
    Taxonomic identifieri394503 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001349: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3535Sequence AnalysisAdd
    BLAST
    Chaini36 – 725690Endoglucanase GPRO_0000007947Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi394503.Ccel_0731.

    Structurei

    Secondary structure

    1
    725
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi41 – 5515
    Beta strandi65 – 684
    Turni74 – 774
    Helixi78 – 803
    Beta strandi91 – 933
    Helixi98 – 12225
    Helixi125 – 14117
    Beta strandi149 – 1546
    Helixi156 – 1605
    Helixi166 – 1683
    Beta strandi175 – 1784
    Beta strandi180 – 1823
    Helixi185 – 20218
    Turni203 – 2053
    Helixi207 – 22721
    Turni235 – 2395
    Helixi246 – 26015
    Helixi263 – 2719
    Helixi272 – 2754
    Beta strandi282 – 2854
    Helixi297 – 30812
    Helixi311 – 32414
    Beta strandi345 – 3473
    Helixi348 – 36417
    Helixi370 – 3723
    Helixi373 – 38816
    Turni389 – 3913
    Beta strandi399 – 4024
    Helixi410 – 4134
    Beta strandi416 – 4183
    Beta strandi422 – 4254
    Helixi451 – 4544
    Helixi458 – 47518
    Beta strandi493 – 50412
    Beta strandi507 – 51610
    Beta strandi527 – 5359
    Helixi537 – 5415
    Helixi546 – 5483
    Beta strandi549 – 5524
    Beta strandi556 – 5583
    Beta strandi567 – 5704
    Helixi571 – 5733
    Beta strandi575 – 5817
    Beta strandi589 – 5913
    Turni592 – 5954
    Beta strandi596 – 6049
    Helixi614 – 6163
    Helixi618 – 6203
    Beta strandi625 – 6284
    Beta strandi633 – 6353
    Beta strandi637 – 6393
    Beta strandi642 – 6465

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G87X-ray1.60A/B36-649[»]
    1GA2X-ray1.70A/B36-649[»]
    1K72X-ray1.80A/B36-649[»]
    1KFGX-ray1.90A/B36-649[»]
    ProteinModelPortaliP37700.
    SMRiP37700. Positions 37-649.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP37700.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini489 – 650162CBM3PROSITE-ProRule annotationAdd
    BLAST
    Repeati664 – 687241Add
    BLAST
    Repeati696 – 719242Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni664 – 719562 X 24 AA approximate repeatsAdd
    BLAST

    Domaini

    A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

    Sequence similaritiesi

    Contains 1 CBM3 (carbohydrate binding type-3) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG05134.
    HOGENOMiHOG000021032.
    OMAiRKEVQFR.
    OrthoDBiEOG6KQ6BP.

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    1.50.10.10. 1 hit.
    2.60.40.710. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR001956. CBD_3.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR018242. Dockerin_1.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view]
    PfamiPF00942. CBM_3. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SMARTiSM01067. CBM_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF49384. SSF49384. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEiPS51172. CBM3. 1 hit.
    PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P37700-1 [UniParc]FASTAAdd to Basket

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    MLKTKRKLTK AIGVALSISI LSSLVSFIPQ TNTYAAGTYN YGEALQKSIM    50
    FYEFQRSGDL PADKRDNWRD DSGMKDGSDV GVDLTGGWYD AGDHVKFNLP 100
    MSYTSAMLAW SLYEDKDAYD KSGQTKYIMD GIKWANDYFI KCNPTPGVYY 150
    YQVGDGGKDH SWWGPAEVMQ MERPSFKVDA SKPGSAVCAS TAASLASAAV 200
    VFKSSDPTYA EKCISHAKNL FDMADKAKSD AGYTAASGYY SSSSFYDDLS 250
    WAAVWLYLAT NDSTYLDKAE SYVPNWGKEQ QTDIIAYKWG QCWDDVHYGA 300
    ELLLAKLTNK QLYKDSIEMN LDFWTTGVNG TRVSYTPKGL AWLFQWGSLR 350
    HATTQAFLAG VYAEWEGCTP SKVSVYKDFL KSQIDYALGS TGRSFVVGYG 400
    VNPPQHPHHR TAHGSWTDQM TSPTYHRHTI YGALVGGPDN ADGYTDEINN 450
    YVNNEIACDY NAGFTGALAK MYKHSGGDPI PNFKAIEKIT NDEVIIKAGL 500
    NSTGPNYTEI KAVVYNQTGW PARVTDKISF KYFMDLSEIV AAGIDPLSLV 550
    TSSNYSEGKN TKVSGVLPWD VSNNVYYVNV DLTGENIYPG GQSACRREVQ 600
    FRIAAPQGTT YWNPKNDFSY DGLPTTSTVN TVTNIPVYDN GVKVFGNEPA 650
    GGSENPDPEI LYGDVNSDKN VDALDFAALK KYLLGGTSSI DVKAADTYKD 700
    GNIDAIDMAT LKKYLLGTIT QLPQG 725
    Length:725
    Mass (Da):79,776
    Last modified:May 26, 2009 - v2
    Checksum:i1C87FE4D03797C41
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti609 – 6102TT → RR in AAA73868. (PubMed:1398087)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87018 Genomic DNA. Translation: AAA73868.1.
    CP001348 Genomic DNA. Translation: ACL75110.1.
    PIRiJC1300.
    RefSeqiYP_002505090.1. NC_011898.1.

    Genome annotation databases

    EnsemblBacteriaiACL75110; ACL75110; Ccel_0731.
    GeneIDi7309585.
    KEGGicce:Ccel_0731.
    PATRICi19432259. VBICloCel57783_0756.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87018 Genomic DNA. Translation: AAA73868.1 .
    CP001348 Genomic DNA. Translation: ACL75110.1 .
    PIRi JC1300.
    RefSeqi YP_002505090.1. NC_011898.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1G87 X-ray 1.60 A/B 36-649 [» ]
    1GA2 X-ray 1.70 A/B 36-649 [» ]
    1K72 X-ray 1.80 A/B 36-649 [» ]
    1KFG X-ray 1.90 A/B 36-649 [» ]
    ProteinModelPortali P37700.
    SMRi P37700. Positions 37-649.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 394503.Ccel_0731.

    Protein family/group databases

    CAZyi CBM3. Carbohydrate-Binding Module Family 3.
    GH9. Glycoside Hydrolase Family 9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL75110 ; ACL75110 ; Ccel_0731 .
    GeneIDi 7309585.
    KEGGi cce:Ccel_0731.
    PATRICi 19432259. VBICloCel57783_0756.

    Phylogenomic databases

    eggNOGi NOG05134.
    HOGENOMi HOG000021032.
    OMAi RKEVQFR.
    OrthoDBi EOG6KQ6BP.

    Enzyme and pathway databases

    UniPathwayi UPA00696 .
    BioCyci CCEL394503:GJET-752-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P37700.

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    1.50.10.10. 1 hit.
    2.60.40.710. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR001956. CBD_3.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR018242. Dockerin_1.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view ]
    Pfami PF00942. CBM_3. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SMARTi SM01067. CBM_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF49384. SSF49384. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEi PS51172. CBM3. 1 hit.
    PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum."
      Bagnara-Tardif C., Gaudin C., Belaich A., Hoest P., Citard T., Belaich J.-P.
      Gene 119:17-28(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.

    Entry informationi

    Entry nameiGUNG_CLOCE
    AccessioniPrimary (citable) accession number: P37700
    Secondary accession number(s): B8I7V3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3