Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endoglucanase G

Gene

celCCG

Organism
Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathwayi: cellulose degradation

This protein is involved in the pathway cellulose degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway cellulose degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei408By similarity1
Active sitei446By similarity1
Active sitei455By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayiUPA00696.

Protein family/group databases

CAZyiCBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase G (EC:3.2.1.4)
Alternative name(s):
Cellulase G
EGCCG
Endo-1,4-beta-glucanase G
Gene namesi
Name:celCCG
Ordered Locus Names:Ccel_0731
OrganismiClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Taxonomic identifieri394503 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000001349 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000000794736 – 725Endoglucanase GAdd BLAST690

Interactioni

Protein-protein interaction databases

STRINGi394503.Ccel_0731.

Structurei

Secondary structure

1725
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi41 – 55Combined sources15
Beta strandi65 – 68Combined sources4
Turni74 – 77Combined sources4
Helixi78 – 80Combined sources3
Beta strandi91 – 93Combined sources3
Helixi98 – 122Combined sources25
Helixi125 – 141Combined sources17
Beta strandi149 – 154Combined sources6
Helixi156 – 160Combined sources5
Helixi166 – 168Combined sources3
Beta strandi175 – 178Combined sources4
Beta strandi180 – 182Combined sources3
Helixi185 – 202Combined sources18
Turni203 – 205Combined sources3
Helixi207 – 227Combined sources21
Turni235 – 239Combined sources5
Helixi246 – 260Combined sources15
Helixi263 – 271Combined sources9
Helixi272 – 275Combined sources4
Beta strandi282 – 285Combined sources4
Helixi297 – 308Combined sources12
Helixi311 – 324Combined sources14
Beta strandi345 – 347Combined sources3
Helixi348 – 364Combined sources17
Helixi370 – 372Combined sources3
Helixi373 – 388Combined sources16
Turni389 – 391Combined sources3
Beta strandi399 – 402Combined sources4
Helixi410 – 413Combined sources4
Beta strandi416 – 418Combined sources3
Beta strandi422 – 425Combined sources4
Helixi451 – 454Combined sources4
Helixi458 – 475Combined sources18
Beta strandi493 – 504Combined sources12
Beta strandi507 – 516Combined sources10
Beta strandi527 – 535Combined sources9
Helixi537 – 541Combined sources5
Helixi546 – 548Combined sources3
Beta strandi549 – 552Combined sources4
Beta strandi556 – 558Combined sources3
Beta strandi567 – 570Combined sources4
Helixi571 – 573Combined sources3
Beta strandi575 – 581Combined sources7
Beta strandi589 – 591Combined sources3
Turni592 – 595Combined sources4
Beta strandi596 – 604Combined sources9
Helixi614 – 616Combined sources3
Helixi618 – 620Combined sources3
Beta strandi625 – 628Combined sources4
Beta strandi633 – 635Combined sources3
Beta strandi637 – 639Combined sources3
Beta strandi642 – 646Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G87X-ray1.60A/B36-649[»]
1GA2X-ray1.70A/B36-649[»]
1K72X-ray1.80A/B36-649[»]
1KFGX-ray1.90A/B36-649[»]
ProteinModelPortaliP37700.
SMRiP37700.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37700.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini489 – 650CBM3PROSITE-ProRule annotationAdd BLAST162
Domaini658 – 724DockerinPROSITE-ProRule annotationAdd BLAST67

Sequence similaritiesi

Contains 1 CBM3 (carbohydrate binding type-3) domain.PROSITE-ProRule annotation
Contains 1 dockerin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CYP. Bacteria.
ENOG410XPC9. LUCA.
HOGENOMiHOG000021032.
KOiK01179.
OMAiGEYSKEC.
OrthoDBiPOG091H0908.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.40.710. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
IPR018221. Glyco_hydro_9_His_AS.
[Graphical view]
PfamiPF00942. CBM_3. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTiSM01067. CBM_3. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS51172. CBM3. 1 hit.
PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKTKRKLTK AIGVALSISI LSSLVSFIPQ TNTYAAGTYN YGEALQKSIM
60 70 80 90 100
FYEFQRSGDL PADKRDNWRD DSGMKDGSDV GVDLTGGWYD AGDHVKFNLP
110 120 130 140 150
MSYTSAMLAW SLYEDKDAYD KSGQTKYIMD GIKWANDYFI KCNPTPGVYY
160 170 180 190 200
YQVGDGGKDH SWWGPAEVMQ MERPSFKVDA SKPGSAVCAS TAASLASAAV
210 220 230 240 250
VFKSSDPTYA EKCISHAKNL FDMADKAKSD AGYTAASGYY SSSSFYDDLS
260 270 280 290 300
WAAVWLYLAT NDSTYLDKAE SYVPNWGKEQ QTDIIAYKWG QCWDDVHYGA
310 320 330 340 350
ELLLAKLTNK QLYKDSIEMN LDFWTTGVNG TRVSYTPKGL AWLFQWGSLR
360 370 380 390 400
HATTQAFLAG VYAEWEGCTP SKVSVYKDFL KSQIDYALGS TGRSFVVGYG
410 420 430 440 450
VNPPQHPHHR TAHGSWTDQM TSPTYHRHTI YGALVGGPDN ADGYTDEINN
460 470 480 490 500
YVNNEIACDY NAGFTGALAK MYKHSGGDPI PNFKAIEKIT NDEVIIKAGL
510 520 530 540 550
NSTGPNYTEI KAVVYNQTGW PARVTDKISF KYFMDLSEIV AAGIDPLSLV
560 570 580 590 600
TSSNYSEGKN TKVSGVLPWD VSNNVYYVNV DLTGENIYPG GQSACRREVQ
610 620 630 640 650
FRIAAPQGTT YWNPKNDFSY DGLPTTSTVN TVTNIPVYDN GVKVFGNEPA
660 670 680 690 700
GGSENPDPEI LYGDVNSDKN VDALDFAALK KYLLGGTSSI DVKAADTYKD
710 720
GNIDAIDMAT LKKYLLGTIT QLPQG
Length:725
Mass (Da):79,776
Last modified:May 26, 2009 - v2
Checksum:i1C87FE4D03797C41
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti609 – 610TT → RR in AAA73868 (PubMed:1398087).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87018 Genomic DNA. Translation: AAA73868.1.
CP001348 Genomic DNA. Translation: ACL75110.1.
PIRiJC1300.
RefSeqiWP_015924277.1. NC_011898.1.

Genome annotation databases

EnsemblBacteriaiACL75110; ACL75110; Ccel_0731.
KEGGicce:Ccel_0731.
PATRICi19432259. VBICloCel57783_0756.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87018 Genomic DNA. Translation: AAA73868.1.
CP001348 Genomic DNA. Translation: ACL75110.1.
PIRiJC1300.
RefSeqiWP_015924277.1. NC_011898.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G87X-ray1.60A/B36-649[»]
1GA2X-ray1.70A/B36-649[»]
1K72X-ray1.80A/B36-649[»]
1KFGX-ray1.90A/B36-649[»]
ProteinModelPortaliP37700.
SMRiP37700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi394503.Ccel_0731.

Protein family/group databases

CAZyiCBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL75110; ACL75110; Ccel_0731.
KEGGicce:Ccel_0731.
PATRICi19432259. VBICloCel57783_0756.

Phylogenomic databases

eggNOGiENOG4105CYP. Bacteria.
ENOG410XPC9. LUCA.
HOGENOMiHOG000021032.
KOiK01179.
OMAiGEYSKEC.
OrthoDBiPOG091H0908.

Enzyme and pathway databases

UniPathwayiUPA00696.

Miscellaneous databases

EvolutionaryTraceiP37700.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.40.710. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
IPR018221. Glyco_hydro_9_His_AS.
[Graphical view]
PfamiPF00942. CBM_3. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTiSM01067. CBM_3. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS51172. CBM3. 1 hit.
PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUNG_CLOCE
AccessioniPrimary (citable) accession number: P37700
Secondary accession number(s): B8I7V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 26, 2009
Last modified: November 30, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.