Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endoglucanase C

Gene

celCCC

Organism
Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathwayi: cellulose degradation

This protein is involved in the pathway cellulose degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway cellulose degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei99Proton donorBy similarity1
Active sitei155NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCCEL394503:G1GUL-746-MONOMER
UniPathwayiUPA00696

Protein family/group databases

CAZyiGH8 Glycoside Hydrolase Family 8

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase C (EC:3.2.1.4)
Alternative name(s):
Cellulase C
EGCCC
Endo-1,4-beta-glucanase C
Gene namesi
Name:celCCC
Ordered Locus Names:Ccel_0730
OrganismiClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Taxonomic identifieri394503 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000001349 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 321 PublicationAdd BLAST32
ChainiPRO_000000793633 – 460Endoglucanase CAdd BLAST428

Post-translational modificationi

There are two forms of the cellulase. The shorter form lacks probably the C-terminal reiterated domains.

Proteomic databases

PRIDEiP37699

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi394503.Ccel_0730

Structurei

3D structure databases

ProteinModelPortaliP37699
SMRiP37699
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini400 – 460DockerinPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F5Q Bacteria
ENOG410Y1JM LUCA
HOGENOMiHOG000069581
OMAiEAHGYGM
OrthoDBiPOG091H07QZ

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR002105 Dockerin_1_rpt
IPR016134 Dockerin_dom
IPR036439 Dockerin_dom_sf
IPR018247 EF_Hand_1_Ca_BS
IPR002037 Glyco_hydro_8
IPR019834 Glyco_hydro_8_CS
PfamiView protein in Pfam
PF00404 Dockerin_1, 2 hits
PF01270 Glyco_hydro_8, 1 hit
PRINTSiPR00735 GLHYDRLASE8
SUPFAMiSSF48208 SSF48208, 1 hit
SSF63446 SSF63446, 1 hit
PROSITEiView protein in PROSITE
PS00448 CLOS_CELLULOSOME_RPT, 1 hit
PS51766 DOCKERIN, 1 hit
PS00018 EF_HAND_1, 2 hits
PS00812 GLYCOSYL_HYDROL_F8, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKGSSLKRF KSLVMAAIFS VSIISTAIAS SAADQIPFPY DAKYPNGAYS
60 70 80 90 100
CLADSQSIGN NLVRSEWEQW KSAHITSNGA RGYKRVQRDA STNYDTVSEG
110 120 130 140 150
LGYGLLLSVY FGEQQLFDDL YRYVKVFLNS NGLMSWRIDS SGNIMGKDSI
160 170 180 190 200
GAATDADEDI AVSLVFAHKK WGTSGGFNYQ TEAKNYINNI YNKMVEPGTY
210 220 230 240 250
VIKAGDTWGG SNVTNPSYFA PAWYRIFADF TGNSGWINVA NKCYEIADKA
260 270 280 290 300
RNSNTGLVPD WCTANGTPAS GQGFDFYYDA IRYQWRAAID YSWYGTAKAK
310 320 330 340 350
THCDAISNFF KNIGYANIKD GYTISGSQIS SNHTATFVSC AAAAAMTGTD
360 370 380 390 400
TTYAKNIYNE CVKVKDSGNY TYFGNTLRMM VLLYTTGNFP NLYTYNSQPK
410 420 430 440 450
PDLKGDVNND GAIDALDIAA LKKAILTQTT SNISLTNADM NNDGNIDAID
460
FAQLKVKLLN
Length:460
Mass (Da):50,483
Last modified:May 26, 2009 - v2
Checksum:i2F31E1F6CB41504F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91S → T in AAA73867 (PubMed:1398087).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87018 Genomic DNA Translation: AAA73867.1
CP001348 Genomic DNA Translation: ACL75109.1
PIRiJC1299
RefSeqiWP_015924276.1, NC_011898.1

Genome annotation databases

EnsemblBacteriaiACL75109; ACL75109; Ccel_0730
KEGGicce:Ccel_0730

Similar proteinsi

Entry informationi

Entry nameiGUNC_CLOCE
AccessioniPrimary (citable) accession number: P37699
Secondary accession number(s): B8I7V2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 26, 2009
Last modified: May 23, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health