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P37699

- GUNC_CLOCE

UniProt

P37699 - GUNC_CLOCE

Protein

Endoglucanase C

Gene

celCCC

Organism
Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (26 May 2009)
      Previous versions | rss
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    Functioni

    The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei99 – 991Proton donorBy similarity
    Active sitei155 – 1551NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCCEL394503:GJET-751-MONOMER.
    UniPathwayiUPA00696.

    Protein family/group databases

    CAZyiGH8. Glycoside Hydrolase Family 8.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase C (EC:3.2.1.4)
    Alternative name(s):
    Cellulase C
    EGCCC
    Endo-1,4-beta-glucanase C
    Gene namesi
    Name:celCCC
    Ordered Locus Names:Ccel_0730
    OrganismiClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
    Taxonomic identifieri394503 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001349: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 32321 PublicationAdd
    BLAST
    Chaini33 – 460428Endoglucanase CPRO_0000007936Add
    BLAST

    Post-translational modificationi

    There are two forms of the cellulase. The shorter form lacks probably the C-terminal reiterated domains.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi394503.Ccel_0730.

    Structurei

    3D structure databases

    ProteinModelPortaliP37699.
    SMRiP37699. Positions 39-391.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati406 – 429241Add
    BLAST
    Repeati439 – 460222Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni406 – 460552 X 24 AA approximate repeatsAdd
    BLAST

    Domaini

    A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG3405.
    HOGENOMiHOG000069581.
    OMAiGNFPNLY.
    OrthoDBiEOG67Q9BX.

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002037. Glyco_hydro_8.
    IPR019834. Glyco_hydro_8_CS.
    [Graphical view]
    PfamiPF00404. Dockerin_1. 2 hits.
    PF01270. Glyco_hydro_8. 1 hit.
    [Graphical view]
    PRINTSiPR00735. GLHYDRLASE8.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS00812. GLYCOSYL_HYDROL_F8. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P37699-1 [UniParc]FASTAAdd to Basket

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    MIKGSSLKRF KSLVMAAIFS VSIISTAIAS SAADQIPFPY DAKYPNGAYS    50
    CLADSQSIGN NLVRSEWEQW KSAHITSNGA RGYKRVQRDA STNYDTVSEG 100
    LGYGLLLSVY FGEQQLFDDL YRYVKVFLNS NGLMSWRIDS SGNIMGKDSI 150
    GAATDADEDI AVSLVFAHKK WGTSGGFNYQ TEAKNYINNI YNKMVEPGTY 200
    VIKAGDTWGG SNVTNPSYFA PAWYRIFADF TGNSGWINVA NKCYEIADKA 250
    RNSNTGLVPD WCTANGTPAS GQGFDFYYDA IRYQWRAAID YSWYGTAKAK 300
    THCDAISNFF KNIGYANIKD GYTISGSQIS SNHTATFVSC AAAAAMTGTD 350
    TTYAKNIYNE CVKVKDSGNY TYFGNTLRMM VLLYTTGNFP NLYTYNSQPK 400
    PDLKGDVNND GAIDALDIAA LKKAILTQTT SNISLTNADM NNDGNIDAID 450
    FAQLKVKLLN 460
    Length:460
    Mass (Da):50,483
    Last modified:May 26, 2009 - v2
    Checksum:i2F31E1F6CB41504F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti91 – 911S → T in AAA73867. (PubMed:1398087)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87018 Genomic DNA. Translation: AAA73867.1.
    CP001348 Genomic DNA. Translation: ACL75109.1.
    PIRiJC1299.
    RefSeqiWP_015924276.1. NC_011898.1.
    YP_002505089.1. NC_011898.1.

    Genome annotation databases

    EnsemblBacteriaiACL75109; ACL75109; Ccel_0730.
    GeneIDi7309584.
    KEGGicce:Ccel_0730.
    PATRICi19432257. VBICloCel57783_0755.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87018 Genomic DNA. Translation: AAA73867.1 .
    CP001348 Genomic DNA. Translation: ACL75109.1 .
    PIRi JC1299.
    RefSeqi WP_015924276.1. NC_011898.1.
    YP_002505089.1. NC_011898.1.

    3D structure databases

    ProteinModelPortali P37699.
    SMRi P37699. Positions 39-391.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 394503.Ccel_0730.

    Protein family/group databases

    CAZyi GH8. Glycoside Hydrolase Family 8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL75109 ; ACL75109 ; Ccel_0730 .
    GeneIDi 7309584.
    KEGGi cce:Ccel_0730.
    PATRICi 19432257. VBICloCel57783_0755.

    Phylogenomic databases

    eggNOGi COG3405.
    HOGENOMi HOG000069581.
    OMAi GNFPNLY.
    OrthoDBi EOG67Q9BX.

    Enzyme and pathway databases

    UniPathwayi UPA00696 .
    BioCyci CCEL394503:GJET-751-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002037. Glyco_hydro_8.
    IPR019834. Glyco_hydro_8_CS.
    [Graphical view ]
    Pfami PF00404. Dockerin_1. 2 hits.
    PF01270. Glyco_hydro_8. 1 hit.
    [Graphical view ]
    PRINTSi PR00735. GLHYDRLASE8.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS00812. GLYCOSYL_HYDROL_F8. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum."
      Bagnara-Tardif C., Gaudin C., Belaich A., Hoest P., Citard T., Belaich J.-P.
      Gene 119:17-28(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.
    3. "Purification and characterization of endoglucanase C from Clostridium cellulolyticum. Catalytic comparison with endoglucanase A."
      Fierobe H.-P., Bagnara-Tardif C., Gaudin C., Guerlesquin F., Sauve P., Belaich A., Belaich J.-P.
      Eur. J. Biochem. 217:557-565(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 33-37, CHARACTERIZATION.
      Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.

    Entry informationi

    Entry nameiGUNC_CLOCE
    AccessioniPrimary (citable) accession number: P37699
    Secondary accession number(s): B8I7V2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3