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P37698

- GUNF_CLOCE

UniProt

P37698 - GUNF_CLOCE

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Protein

Endoglucanase F

Gene
celCCF, Ccel_0729
Organism
Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Probable endoglucanase involved in the degradation of cellulose or related beta-glucans.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCCEL394503:GJET-750-MONOMER.

Protein family/group databases

CAZyiGH48. Glycoside Hydrolase Family 48.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase F (EC:3.2.1.4)
Alternative name(s):
Cellulase F
EGCCF
Endo-1,4-beta-glucanase F
Gene namesi
Name:celCCF
Ordered Locus Names:Ccel_0729
OrganismiClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Taxonomic identifieri394503 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001349: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed predictionAdd
BLAST
Chaini30 – 722693Endoglucanase FPRO_0000008026Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi394503.Ccel_0729.

Structurei

Secondary structure

1
722
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi36 – 5015
Helixi52 – 543
Beta strandi65 – 673
Beta strandi73 – 775
Beta strandi80 – 823
Helixi83 – 10018
Helixi104 – 11613
Turni121 – 1233
Helixi126 – 1294
Helixi147 – 1493
Beta strandi151 – 1533
Helixi166 – 1738
Beta strandi174 – 1774
Beta strandi182 – 1865
Beta strandi190 – 1923
Beta strandi202 – 2076
Helixi217 – 2193
Beta strandi223 – 2275
Beta strandi229 – 2335
Helixi238 – 2403
Beta strandi251 – 2566
Helixi258 – 27619
Turni277 – 2793
Helixi283 – 29513
Helixi296 – 3005
Beta strandi310 – 3145
Helixi318 – 3203
Beta strandi330 – 3378
Beta strandi340 – 3434
Beta strandi346 – 3494
Helixi350 – 3523
Helixi355 – 3639
Helixi365 – 3673
Beta strandi370 – 3734
Helixi374 – 39118
Beta strandi395 – 3973
Beta strandi402 – 4076
Turni408 – 4114
Beta strandi424 – 4296
Turni433 – 4386
Helixi442 – 45817
Helixi461 – 47515
Beta strandi486 – 49611
Turni503 – 5053
Beta strandi514 – 5218
Helixi524 – 54118
Helixi544 – 56017
Beta strandi561 – 5633
Beta strandi570 – 5723
Turni574 – 5774
Helixi578 – 5814
Helixi606 – 6094
Helixi611 – 6155
Helixi619 – 6279
Beta strandi633 – 6353
Helixi639 – 65517

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F9DX-ray2.30A30-658[»]
1F9OX-ray2.50A30-658[»]
1FAEX-ray2.00A30-658[»]
1FBOX-ray2.30A30-658[»]
1FBWX-ray2.00A30-658[»]
1FCEX-ray2.00A30-658[»]
1G9GX-ray1.90A30-658[»]
1G9JX-ray1.90A30-658[»]
2QNOX-ray2.00A30-658[»]
ProteinModelPortaliP37698.
SMRiP37698. Positions 30-658.

Miscellaneous databases

EvolutionaryTraceiP37698.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati667 – 690241Add
BLAST
Repeati699 – 722242Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni667 – 722562 X 24 AA approximate repeatsAdd
BLAST

Domaini

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG10655.
HOGENOMiHOG000020369.
OMAiTYHRFWS.
OrthoDBiEOG64V28H.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
1.50.10.10. 4 hits.
4.10.870.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR027390. Endoglucanase_F_dom3.
IPR000556. Glyco_hydro_48F.
[Graphical view]
PfamiPF00404. Dockerin_1. 2 hits.
PF02011. Glyco_hydro_48. 1 hit.
[Graphical view]
PRINTSiPR00844. GLHYDRLASE48.
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37698-1 [UniParc]FASTAAdd to Basket

« Hide

MSKNFKRVGA VAVAAAMSLS IMATTSINAA SSPANKVYQD RFESMYSKIK    50
DPANGYFSEQ GIPYHSIETL MVEAPDYGHV TTSEAMSYYM WLEAMHGRFS 100
GDFTGFDKSW SVTEQYLIPT EKDQPNTSMS RYDANKPATY APEFQDPSKY 150
PSPLDTSQPV GRDPINSQLT SAYGTSMLYG MHWILDVDNW YGFGARADGT 200
SKPSYINTFQ RGEQESTWET IPQPCWDEHK FGGQYGFLDL FTKDTGTPAK 250
QFKYTNAPDA DARAVQATYW ADQWAKEQGK SVSTSVGKAT KMGDYLRYSF 300
FDKYFRKIGQ PSQAGTGYDA AHYLLSWYYA WGGGIDSTWS WIIGSSHNHF 350
GYQNPFAAWV LSTDANFKPK SSNGASDWAK SLDRQLEFYQ WLQSAEGAIA 400
GGATNSWNGR YEAVPSGTST FYGMGYVENP VYADPGSNTW FGMQVWSMQR 450
VAELYYKTGD ARAKKLLDKW AKWINGEIKF NADGTFQIPS TIDWEGQPDT 500
WNPTQGYTGN ANLHVKVVNY GTDLGCASSL ANTLTYYAAK SGDETSRQNA 550
QKLLDAMWNN YSDSKGISTV EQRGDYHRFL DQEVFVPAGW TGKMPNGDVI 600
KSGVKFIDIR SKYKQDPEWQ TMVAALQAGQ VPTQRLHRFW AQSEFAVANG 650
VYAILFPDQG PEKLLGDVNG DETVDAIDLA ILKKYLLNSS TTINTANADM 700
NSDNAIDAID YALLKKALLS IQ 722
Length:722
Mass (Da):80,544
Last modified:October 1, 1996 - v2
Checksum:i5E48F85319D70AE5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U30321 Genomic DNA. Translation: AAB41452.1.
CP001348 Genomic DNA. Translation: ACL75108.1.
M87018 Genomic DNA. Translation: AAA73866.1.
PIRiPC1139.
RefSeqiWP_015924275.1. NC_011898.1.
YP_002505088.1. NC_011898.1.

Genome annotation databases

EnsemblBacteriaiACL75108; ACL75108; Ccel_0729.
GeneIDi7309583.
KEGGicce:Ccel_0729.
PATRICi19432255. VBICloCel57783_0754.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U30321 Genomic DNA. Translation: AAB41452.1 .
CP001348 Genomic DNA. Translation: ACL75108.1 .
M87018 Genomic DNA. Translation: AAA73866.1 .
PIRi PC1139.
RefSeqi WP_015924275.1. NC_011898.1.
YP_002505088.1. NC_011898.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1F9D X-ray 2.30 A 30-658 [» ]
1F9O X-ray 2.50 A 30-658 [» ]
1FAE X-ray 2.00 A 30-658 [» ]
1FBO X-ray 2.30 A 30-658 [» ]
1FBW X-ray 2.00 A 30-658 [» ]
1FCE X-ray 2.00 A 30-658 [» ]
1G9G X-ray 1.90 A 30-658 [» ]
1G9J X-ray 1.90 A 30-658 [» ]
2QNO X-ray 2.00 A 30-658 [» ]
ProteinModelPortali P37698.
SMRi P37698. Positions 30-658.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 394503.Ccel_0729.

Protein family/group databases

CAZyi GH48. Glycoside Hydrolase Family 48.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL75108 ; ACL75108 ; Ccel_0729 .
GeneIDi 7309583.
KEGGi cce:Ccel_0729.
PATRICi 19432255. VBICloCel57783_0754.

Phylogenomic databases

eggNOGi NOG10655.
HOGENOMi HOG000020369.
OMAi TYHRFWS.
OrthoDBi EOG64V28H.

Enzyme and pathway databases

BioCyci CCEL394503:GJET-750-MONOMER.

Miscellaneous databases

EvolutionaryTracei P37698.

Family and domain databases

Gene3Di 1.10.1330.10. 1 hit.
1.50.10.10. 4 hits.
4.10.870.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR027390. Endoglucanase_F_dom3.
IPR000556. Glyco_hydro_48F.
[Graphical view ]
Pfami PF00404. Dockerin_1. 2 hits.
PF02011. Glyco_hydro_48. 1 hit.
[Graphical view ]
PRINTSi PR00844. GLHYDRLASE48.
SUPFAMi SSF48208. SSF48208. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli."
    Reverbel-Leroy C., Belaich A., Bernadac A., Gaudin C., Belaich J.-P., Tardif C.
    Microbiology 142:1013-1023(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.
  3. "Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum."
    Bagnara-Tardif C., Gaudin C., Belaich A., Hoest P., Citard T., Belaich J.-P.
    Gene 119:17-28(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 387-722.

Entry informationi

Entry nameiGUNF_CLOCE
AccessioniPrimary (citable) accession number: P37698
Secondary accession number(s): B8I7V1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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