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Protein

Probable endoglucanase

Gene

cmcAX

Organism
Komagataeibacter hansenii (Gluconacetobacter hansenii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme capable of hydrolyzing carboxy-methyl-cellulose (CMC).

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei57 – 571Proton donorBy similarity
Active sitei114 – 1141NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1558-MONOMER.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:cmcAX
OrganismiKomagataeibacter hansenii (Gluconacetobacter hansenii)
Taxonomic identifieri436 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeKomagataeibacter

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20201 PublicationAdd
BLAST
Chaini21 – 342322Probable endoglucanasePRO_0000007930Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi714995.GXY_04262.

Structurei

Secondary structure

1
342
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 3813Combined sources
Beta strandi48 – 514Combined sources
Beta strandi53 – 553Combined sources
Helixi56 – 6914Combined sources
Helixi72 – 8514Combined sources
Beta strandi89 – 924Combined sources
Beta strandi96 – 994Combined sources
Beta strandi102 – 1065Combined sources
Helixi113 – 13018Combined sources
Helixi133 – 15018Combined sources
Beta strandi151 – 1544Combined sources
Beta strandi157 – 1604Combined sources
Beta strandi164 – 1663Combined sources
Beta strandi170 – 1756Combined sources
Helixi177 – 1793Combined sources
Helixi182 – 19211Combined sources
Helixi196 – 21015Combined sources
Turni214 – 2174Combined sources
Beta strandi221 – 2266Combined sources
Turni227 – 2293Combined sources
Beta strandi232 – 2343Combined sources
Beta strandi240 – 2423Combined sources
Turni244 – 2474Combined sources
Helixi248 – 2558Combined sources
Helixi261 – 27414Combined sources
Turni285 – 2873Combined sources
Beta strandi292 – 2943Combined sources
Helixi297 – 3059Combined sources
Helixi317 – 3193Combined sources
Helixi323 – 33917Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WZZX-ray1.65A21-342[»]
ProteinModelPortaliP37696.
SMRiP37696. Positions 23-341.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37696.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P37696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVMAAMGGA QVLSSTGAFA DPAPDAVAQQ WAIFRAKYLR PSGRVVDTGN
60 70 80 90 100
GGESHSEGQG YGMLFAASAG DLASFQSMWM WARTNLQHTN DKLFSWRFLK
110 120 130 140 150
GHQPPVPDKN NATDGDLLIA LALGRAGKRF QRPDYIQDAM AIYGDVLNLM
160 170 180 190 200
TMKAGPYVVL MPGAVGFTKK DSVILNLSYY VMPSLLQAFD LTADPRWRQV
210 220 230 240 250
MEDGIRLVSA GRFGQWRLPP DWLAVNRATG ALSIASGWPP RFSYDAIRVP
260 270 280 290 300
LYFYWAHMLA PNVLADFTRF WNNFGANALP GWVDLTTGAR SPYNAPPGYL
310 320 330 340
AVAECTGLDS AGELPTLDHA PDYYSAALTL LVYIARAEET IK
Length:342
Mass (Da):37,443
Last modified:October 1, 1994 - v1
Checksum:i85FF9BDF8B979F81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96060 Unassigned DNA. Translation: AAA16969.1.
PIRiA36963.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96060 Unassigned DNA. Translation: AAA16969.1.
PIRiA36963.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WZZX-ray1.65A21-342[»]
ProteinModelPortaliP37696.
SMRiP37696. Positions 23-341.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi714995.GXY_04262.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1558-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP37696.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A new gene required for cellulose production and a gene encoding cellulolytic activity in Acetobacter xylinum are colocalized with the bcs operon."
    Standal R., Iversen T.-G., Coucheron D.H., Fjaervik E., Blatny J.M., Valla S.
    J. Bacteriol. 176:665-672(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 21-34.
    Strain: ATCC 23769 / NCIMB 8246.

Entry informationi

Entry nameiGUNA_KOMHA
AccessioniPrimary (citable) accession number: P37696
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 24, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.