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Protein

ADP-heptose--LPS heptosyltransferase 2

Gene

rfaF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • ADP-heptose-lipopolysaccharide heptosyltransferase activity Source: CACAO
  • transferase activity, transferring glycosyl groups Source: EcoCyc

GO - Biological processi

  • lipopolysaccharide core region biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG12210-MONOMER.
ECOL316407:JW3595-MONOMER.
MetaCyc:EG12210-MONOMER.
BRENDAi2.4.99.B7. 2026.
UniPathwayiUPA00958.

Protein family/group databases

CAZyiGT9. Glycosyltransferase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-heptose--LPS heptosyltransferase 2 (EC:2.-.-.-)
Alternative name(s):
ADP-heptose--LPS heptosyltransferase II
Gene namesi
Name:rfaF
Synonyms:waaF
Ordered Locus Names:b3620, JW3595
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12210. rfaF.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002072621 – 348ADP-heptose--LPS heptosyltransferase 2Add BLAST348

Proteomic databases

PaxDbiP37692.
PRIDEiP37692.

Interactioni

Protein-protein interaction databases

BioGridi4263306. 335 interactors.
DIPiDIP-10667N.
IntActiP37692. 11 interactors.
STRINGi511145.b3620.

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi11 – 27Combined sources17
Beta strandi32 – 37Combined sources6
Helixi39 – 41Combined sources3
Helixi42 – 45Combined sources4
Beta strandi51 – 56Combined sources6
Helixi66 – 75Combined sources10
Turni76 – 80Combined sources5
Beta strandi82 – 86Combined sources5
Helixi92 – 94Combined sources3
Helixi95 – 99Combined sources5
Beta strandi103 – 108Combined sources6
Turni110 – 112Combined sources3
Turni114 – 116Combined sources3
Beta strandi119 – 121Combined sources3
Turni125 – 127Combined sources3
Helixi131 – 137Combined sources7
Helixi142 – 144Combined sources3
Helixi148 – 150Combined sources3
Helixi164 – 173Combined sources10
Beta strandi178 – 180Combined sources3
Beta strandi182 – 186Combined sources5
Helixi193 – 195Combined sources3
Helixi199 – 211Combined sources13
Beta strandi215 – 218Combined sources4
Helixi222 – 224Combined sources3
Helixi225 – 232Combined sources8
Helixi237 – 240Combined sources4
Beta strandi243 – 245Combined sources3
Turni247 – 249Combined sources3
Helixi252 – 260Combined sources9
Beta strandi262 – 270Combined sources9
Helixi271 – 278Combined sources8
Beta strandi283 – 290Combined sources8
Beta strandi302 – 308Combined sources7
Helixi325 – 328Combined sources4
Helixi332 – 347Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PSWX-ray2.00A1-348[»]
ProteinModelPortaliP37692.
SMRiP37692.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37692.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 9 family.Curated

Phylogenomic databases

eggNOGiENOG4105XA0. Bacteria.
COG0859. LUCA.
HOGENOMiHOG000237541.
InParanoidiP37692.
KOiK02843.
OMAiCLKQRHP.
PhylomeDBiP37692.

Family and domain databases

InterProiIPR002201. Glyco_trans_9.
IPR011910. LipoPS_heptosylTferase-II.
[Graphical view]
PfamiPF01075. Glyco_transf_9. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02195. heptsyl_trn_II. 1 hit.

Sequencei

Sequence statusi: Complete.

P37692-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILVIGPSW VGDMMMSQSL YRTLQARYPQ AIIDVMAPAW CRPLLSRMPE
60 70 80 90 100
VNEAIPMPLG HGALEIGERR KLGHSLREKR YDRAYVLPNS FKSALVPFFA
110 120 130 140 150
GIPHRTGWRG EMRYGLLNDV RVLDKEAWPL MVERYIALAY DKGIMRTAQD
160 170 180 190 200
LPQPLLWPQL QVSEGEKSYT CNQFSLSSER PMIGFCPGAE FGPAKRWPHY
210 220 230 240 250
HYAELAKQLI DEGYQVVLFG SAKDHEAGNE ILAALNTEQQ AWCRNLAGET
260 270 280 290 300
QLDQAVILIA ACKAIVTNDS GLMHVAAALN RPLVALYGPS SPDFTPPLSH
310 320 330 340
KARVIRLITG YHKVRKGDAA EGYHQSLIDI TPQRVLEELN ALLLQEEA
Length:348
Mass (Da):39,042
Last modified:October 1, 1994 - v1
Checksum:iC5F57BC94B15BA6A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16Missing in M33577 (PubMed:2198271).Curated1
Sequence conflicti19 – 20SL → DR in M33577 (PubMed:2198271).Curated2
Sequence conflicti105R → S (PubMed:8478319).Curated1
Sequence conflicti151L → R (PubMed:8478319).Curated1
Sequence conflicti159Q → H (PubMed:8478319).Curated1
Sequence conflicti204 – 205EL → DV (PubMed:8478319).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18597.1.
U00096 Genomic DNA. Translation: AAC76644.1.
AP009048 Genomic DNA. Translation: BAE77672.1.
M33577 Genomic DNA. No translation available.
X62530 Genomic DNA. No translation available.
PIRiS47841.
RefSeqiNP_418077.1. NC_000913.3.
WP_000699219.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76644; AAC76644; b3620.
BAE77672; BAE77672; BAE77672.
GeneIDi948135.
KEGGiecj:JW3595.
eco:b3620.
PATRICi32122727. VBIEscCol129921_3740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18597.1.
U00096 Genomic DNA. Translation: AAC76644.1.
AP009048 Genomic DNA. Translation: BAE77672.1.
M33577 Genomic DNA. No translation available.
X62530 Genomic DNA. No translation available.
PIRiS47841.
RefSeqiNP_418077.1. NC_000913.3.
WP_000699219.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PSWX-ray2.00A1-348[»]
ProteinModelPortaliP37692.
SMRiP37692.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263306. 335 interactors.
DIPiDIP-10667N.
IntActiP37692. 11 interactors.
STRINGi511145.b3620.

Protein family/group databases

CAZyiGT9. Glycosyltransferase Family 9.

Proteomic databases

PaxDbiP37692.
PRIDEiP37692.

Protocols and materials databases

DNASUi948135.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76644; AAC76644; b3620.
BAE77672; BAE77672; BAE77672.
GeneIDi948135.
KEGGiecj:JW3595.
eco:b3620.
PATRICi32122727. VBIEscCol129921_3740.

Organism-specific databases

EchoBASEiEB2124.
EcoGeneiEG12210. rfaF.

Phylogenomic databases

eggNOGiENOG4105XA0. Bacteria.
COG0859. LUCA.
HOGENOMiHOG000237541.
InParanoidiP37692.
KOiK02843.
OMAiCLKQRHP.
PhylomeDBiP37692.

Enzyme and pathway databases

UniPathwayiUPA00958.
BioCyciEcoCyc:EG12210-MONOMER.
ECOL316407:JW3595-MONOMER.
MetaCyc:EG12210-MONOMER.
BRENDAi2.4.99.B7. 2026.

Miscellaneous databases

EvolutionaryTraceiP37692.
PROiP37692.

Family and domain databases

InterProiIPR002201. Glyco_trans_9.
IPR011910. LipoPS_heptosylTferase-II.
[Graphical view]
PfamiPF01075. Glyco_transf_9. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02195. heptsyl_trn_II. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFAF_ECOLI
AccessioniPrimary (citable) accession number: P37692
Secondary accession number(s): Q2M7T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.