Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P37692 (RFAF_ECOLI)

Last modified June 16, 2009. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    ADP-heptose--LPS heptosyltransferase 2
    EC=2.-.-.-
Alternative name(s):
    ADP-heptose--LPS heptosyltransferase II
Gene names
Name: rfaF
Synonyms: waaF
Ordered Locus Names: b3620, JW3595
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Hide | Top

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Pathway

Bacterial outer membrane biogenesis; LPS core biosynthesis.

Sequence similarities

Belongs to the glycosyltransferase 9 family.

Hide | Top

Ontologies

Keywords
   Biological processLipopolysaccharide biosynthesis
   Molecular functionGlycosyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processlipopolysaccharide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functiontransferase activity, transferring glycosyl groups

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348ADP-heptose--LPS heptosyltransferase 2
PRO_0000207262

Experimental info

Sequence conflict161Missing in M33577. Ref.4
Sequence conflict19 – 202SL → DR in M33577. Ref.4
Sequence conflict1051R → S Ref.5
Sequence conflict1511L → R Ref.5
Sequence conflict1591Q → H Ref.5
Sequence conflict204 – 2052EL → DV Ref.5

Secondary structure

1 ...................................................................... 348
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P37692-1 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: C5F57BC94B15BA6A

FASTA34839,042
        10         20         30         40         50         60 
MKILVIGPSW VGDMMMSQSL YRTLQARYPQ AIIDVMAPAW CRPLLSRMPE VNEAIPMPLG 

        70         80         90        100        110        120 
HGALEIGERR KLGHSLREKR YDRAYVLPNS FKSALVPFFA GIPHRTGWRG EMRYGLLNDV 

       130        140        150        160        170        180 
RVLDKEAWPL MVERYIALAY DKGIMRTAQD LPQPLLWPQL QVSEGEKSYT CNQFSLSSER 

       190        200        210        220        230        240 
PMIGFCPGAE FGPAKRWPHY HYAELAKQLI DEGYQVVLFG SAKDHEAGNE ILAALNTEQQ 

       250        260        270        280        290        300 
AWCRNLAGET QLDQAVILIA ACKAIVTNDS GLMHVAAALN RPLVALYGPS SPDFTPPLSH 

       310        320        330        340 
KARVIRLITG YHKVRKGDAA EGYHQSLIDI TPQRVLEELN ALLLQEEA

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Cloning, expression, and characterization of the Escherichia coli K-12 rfaD gene."
Pegues J.C., Chen L., Gordon A.W., Ding L., Coleman W.G. Jr.
J. Bacteriol. 172:4652-4660(1990) [PubMed: 2198271] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-51.
Strain: K12.
[5]"Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis."
Chen L., Coleman W.G. Jr.
J. Bacteriol. 175:2534-2540(1993) [PubMed: 8478319] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 52-348.
Strain: K12.

Hide | Top

Cross-references

Sequence databases

U00039 Genomic DNA. Translation: AAB18597.1.
U00096 Genomic DNA. Translation: AAC76644.1.
AP009048 Genomic DNA. Translation: BAE77672.1.
M33577 Genomic DNA. No translation available.
X62530 Genomic DNA. No translation available.
PIRS47841.
RefSeqAP_004171.1.
NP_418077.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1PSWX-ray2.00A1-348[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:10667N.

Protein family/group databases

CAZyGT9. Glycosyltransferase Family 9.

Genome annotation databases

GeneID948135.
GenomeReviewsGene locus JW3595 in contig AP009048_GR.
Gene locus b3620 in contig U00096_GR.
KEGGecj:JW3595.
eco:b3620.

Organism-specific databases

EchoBASEEB2124.
EcoGeneEG12210. rfaF.
CMRSearch...

Phylogenomic databases

HOGENOMP37692.
OMAP37692. YDQAILL.

Enzyme and pathway databases

BioCycEcoCyc:EG12210-MON.
MetaCyc:EG12210-MON.

Family and domain databases

InterProIPR002201. Glyco_trans_9.
IPR011910. Heptsyl_trn_II.
[Graphical view]
PfamPF01075. Glyco_transf_9. 1 hit.
[Graphical view]
TIGRFAMsTIGR02195. heptsyl_trn_II. 1 hit.
ProtoNetSearch...

Hide | Top

Entry information

Entry nameRFAF_ECOLI
AccessionPrimary (citable) accession number: P37692
Secondary accession number(s): Q2M7T4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents