P37692 (RFAF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-heptose--LPS heptosyltransferase 2
EC=2.-.-.-

Alternative name(s):
ADP-heptose--LPS heptosyltransferase II

Gene names

Name:	rfaF
Synonyms:	waaF
Ordered Locus Names:	b3620, JW3595

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 9 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 348

348

ADP-heptose--LPS heptosyltransferase 2

PRO_0000207262

Experimental info

Sequence conflict

16

1

Missing in M33577. Ref.4

Sequence conflict

19 – 20

2

SL → DR in M33577. Ref.4

Sequence conflict

105

1

R → S Ref.5

Sequence conflict

151

1

L → R Ref.5

Sequence conflict

159

1

Q → H Ref.5

Sequence conflict

204 – 205

2

EL → DV Ref.5

Secondary structure

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348

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Helix

11 – 27

17

Beta strand

32 – 37

6

Helix

39 – 41

3

Helix

42 – 45

4

Beta strand

51 – 56

6

Helix

66 – 75

10

Turn

76 – 80

5

Beta strand

82 – 86

5

Helix

92 – 94

3

Helix

95 – 99

5

Beta strand

103 – 108

6

Turn

110 – 112

3

Turn

114 – 116

3

Beta strand

119 – 121

3

Turn

125 – 127

3

Helix

131 – 137

7

Helix

142 – 144

3

Helix

148 – 150

3

Helix

164 – 173

10

Beta strand

178 – 180

3

Beta strand

182 – 186

5

Helix

193 – 195

3

Helix

199 – 211

13

Beta strand

215 – 218

4

Helix

222 – 224

3

Helix

225 – 232

8

Helix

237 – 240

4

Beta strand

243 – 245

3

Turn

247 – 249

3

Helix

252 – 260

9

Beta strand

262 – 270

9

Helix

271 – 278

8

Beta strand

283 – 290

8

Beta strand

302 – 308

7

Helix

325 – 328

4

Helix

332 – 347

16

Sequences

	Sequence		Length	Mass (Da)
	P37692 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: C5F57BC94B15BA6A	FASTA	348	39,042
	10 20 30 40 50 60 MKILVIGPSW VGDMMMSQSL YRTLQARYPQ AIIDVMAPAW CRPLLSRMPE VNEAIPMPLG 70 80 90 100 110 120 HGALEIGERR KLGHSLREKR YDRAYVLPNS FKSALVPFFA GIPHRTGWRG EMRYGLLNDV 130 140 150 160 170 180 RVLDKEAWPL MVERYIALAY DKGIMRTAQD LPQPLLWPQL QVSEGEKSYT CNQFSLSSER 190 200 210 220 230 240 PMIGFCPGAE FGPAKRWPHY HYAELAKQLI DEGYQVVLFG SAKDHEAGNE ILAALNTEQQ 250 260 270 280 290 300 AWCRNLAGET QLDQAVILIA ACKAIVTNDS GLMHVAAALN RPLVALYGPS SPDFTPPLSH 310 320 330 340 KARVIRLITG YHKVRKGDAA EGYHQSLIDI TPQRVLEELN ALLLQEEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Cloning, expression, and characterization of the Escherichia coli K-12 rfaD gene." Pegues J.C., Chen L., Gordon A.W., Ding L., Coleman W.G. Jr. J. Bacteriol. 172:4652-4660(1990) [PubMed: 2198271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-51. Strain: K12.
[5]	"Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis." Chen L., Coleman W.G. Jr. J. Bacteriol. 175:2534-2540(1993) [PubMed: 8478319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 52-348. Strain: K12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00039 Genomic DNA. Translation: AAB18597.1.
U00096 Genomic DNA. Translation: AAC76644.1.
AP009048 Genomic DNA. Translation: BAE77672.1.
M33577 Genomic DNA. No translation available.
X62530 Genomic DNA. No translation available.

PIR

S47841.

RefSeq

NP_418077.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PSW	X-ray	2.00	A	1-348	[»]

ProteinModelPortal

P37692.

SMR

P37692. Positions 1-348.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10667N.

IntAct

P37692. 11 interactions.

Protein family/group databases

CAZy

GT9. Glycosyltransferase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002675; EBESCP00000002675; EBESCG00000002184.
EBESCT00000002676; EBESCP00000002676; EBESCG00000002184.
EBESCT00000002677; EBESCP00000002677; EBESCG00000002184.
EBESCT00000002678; EBESCP00000002678; EBESCG00000002184.
EBESCT00000015887; EBESCP00000015178; EBESCG00000014947.

GeneID

948135.

GenomeReviews

Gene locus JW3595 in contig AP009048_GR.
Gene locus b3620 in contig U00096_GR.

KEGG

ecj:JW3595.
eco:b3620.

PATRIC

32122727. VBIEscCol129921_3740.

Organism-specific databases

EchoBASE

EB2124.

EcoGene

EG12210. rfaF.

Phylogenomic databases

eggNOG

COG0859.

GeneTree

EBGT00050000010625.

HOGENOM

HBG527482.

OMA

GWKGEMR.

PhylomeDB

P37692.

ProtClustDB

PRK10916.

Enzyme and pathway databases

BioCyc

EcoCyc:EG12210-MONOMER.
MetaCyc:EG12210-MONOMER.

Gene expression databases

Genevestigator

P37692.

Family and domain databases

InterPro

IPR002201. Glyco_trans_9.
IPR011910. LipoPS_heptosylTferase-II.
[Graphical view]

KO

K02843.

Pfam

PF01075. Glyco_transf_9. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02195. Heptsyl_trn_II. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RFAF_ECOLI

Accession

Primary (citable) accession number: P37692
Secondary accession number(s): Q2M7T4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program