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P37689

- GPMI_ECOLI

UniProt

P37689 - GPMI_ECOLI

Protein

2,3-bisphosphoglycerate-independent phosphoglycerate mutase

Gene

gpmI

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.

    Catalytic activityi

    2-phospho-D-glycerate = 3-phospho-D-glycerate.

    Cofactori

    Binds 2 manganese ions per subunit.

    Enzyme regulationi

    Insensitive to vanadate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi14 – 141Manganese 2By similarity
    Active sitei64 – 641Phosphoserine intermediateBy similarity
    Metal bindingi64 – 641Manganese 2By similarity
    Metal bindingi403 – 4031Manganese 1By similarity
    Metal bindingi407 – 4071Manganese 1By similarity
    Metal bindingi444 – 4441Manganese 2By similarity
    Metal bindingi445 – 4451Manganese 2By similarity
    Metal bindingi463 – 4631Manganese 1By similarity

    GO - Molecular functioni

    1. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity Source: EcoCyc
    2. manganese ion binding Source: EcoCyc

    GO - Biological processi

    1. glucose catabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP
    3. response to oxidative stress Source: EcoCyc

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:PGMI-MONOMER.
    ECOL316407:JW3587-MONOMER.
    MetaCyc:PGMI-MONOMER.
    SABIO-RKP37689.
    UniPathwayiUPA00109; UER00186.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC:5.4.2.12)
    Short name:
    BPG-independent PGAM
    Short name:
    Phosphoglyceromutase
    Short name:
    iPGM
    Gene namesi
    Name:gpmI
    Synonyms:pgmI, yibO
    Ordered Locus Names:b3612, JW3587
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12296. gpmI.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5145142,3-bisphosphoglycerate-independent phosphoglycerate mutasePRO_0000212145Add
    BLAST

    Proteomic databases

    PaxDbiP37689.
    PRIDEiP37689.

    2D gel databases

    SWISS-2DPAGEP37689.

    Expressioni

    Developmental stagei

    Expressed most strongly in early exponential growth, with levels falling off as cells reached late log phase and stationary phase.

    Gene expression databases

    GenevestigatoriP37689.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    DIPiDIP-12425N.
    IntActiP37689. 4 interactions.
    STRINGi511145.b3612.

    Structurei

    3D structure databases

    ProteinModelPortaliP37689.
    SMRiP37689. Positions 5-504.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0696.
    HOGENOMiHOG000223664.
    KOiK15633.
    OMAiDACIGRV.
    OrthoDBiEOG6HJ22X.
    PhylomeDBiP37689.

    Family and domain databases

    Gene3Di3.40.1450.10. 1 hit.
    3.40.720.10. 2 hits.
    HAMAPiMF_01038. GpmI.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR011258. BPG-indep_PGM_N.
    IPR006124. Metalloenzyme.
    IPR005995. Pgm_bpd_ind.
    [Graphical view]
    PfamiPF06415. iPGM_N. 1 hit.
    PF01676. Metalloenzyme. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001492. IPGAM. 1 hit.
    SUPFAMiSSF53649. SSF53649. 2 hits.
    SSF64158. SSF64158. 1 hit.
    TIGRFAMsiTIGR01307. pgm_bpd_ind. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P37689-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLVSKKPMVL VILDGYGYRE EQQDNAIFSA KTPVMDALWA NRPHTLIDAS    50
    GLEVGLPDRQ MGNSEVGHVN LGAGRIVYQD LTRLDVEIKD RAFFANPVLT 100
    GAVDKAKNAG KAVHIMGLLS AGGVHSHEDH IMAMVELAAE RGAEKIYLHA 150
    FLDGRDTPPR SAESSLKKFE EKFAALGKGR VASIIGRYYA MDRDNRWDRV 200
    EKAYDLLTLA QGEFQADTAV AGLQAAYARD ENDEFVKATV IRAEGQPDAA 250
    MEDGDALIFM NFRADRAREI TRAFVNADFD GFARKKVVNV DFVMLTEYAA 300
    DIKTAVAYPP ASLVNTFGEW MAKNDKTQLR ISETEKYAHV TFFFNGGVEE 350
    SFKGEDRILI NSPKVATYDL QPEMSSAELT EKLVAAIKSG KYDTIICNYP 400
    NGDMVGHTGV MEAAVKAVEA LDHCVEEVAK AVESVGGQLL ITADHGNAEQ 450
    MRDPATGQAH TAHTNLPVPL IYVGDKNVKA VEGGKLSDIA PTMLSLMGME 500
    IPQEMTGKPL FIVE 514
    Length:514
    Mass (Da):56,194
    Last modified:October 1, 1994 - v1
    Checksum:iBD4173C2BA2CEDD8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00039 Genomic DNA. Translation: AAB18589.1.
    U00096 Genomic DNA. Translation: AAC76636.1.
    AP009048 Genomic DNA. Translation: BAE77680.1.
    PIRiS47833.
    RefSeqiNP_418069.1. NC_000913.3.
    YP_491821.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76636; AAC76636; b3612.
    BAE77680; BAE77680; BAE77680.
    GeneIDi12934300.
    948130.
    KEGGiecj:Y75_p3562.
    eco:b3612.
    PATRICi32122711. VBIEscCol129921_3732.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00039 Genomic DNA. Translation: AAB18589.1 .
    U00096 Genomic DNA. Translation: AAC76636.1 .
    AP009048 Genomic DNA. Translation: BAE77680.1 .
    PIRi S47833.
    RefSeqi NP_418069.1. NC_000913.3.
    YP_491821.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P37689.
    SMRi P37689. Positions 5-504.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-12425N.
    IntActi P37689. 4 interactions.
    STRINGi 511145.b3612.

    2D gel databases

    SWISS-2DPAGE P37689.

    Proteomic databases

    PaxDbi P37689.
    PRIDEi P37689.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76636 ; AAC76636 ; b3612 .
    BAE77680 ; BAE77680 ; BAE77680 .
    GeneIDi 12934300.
    948130.
    KEGGi ecj:Y75_p3562.
    eco:b3612.
    PATRICi 32122711. VBIEscCol129921_3732.

    Organism-specific databases

    EchoBASEi EB2204.
    EcoGenei EG12296. gpmI.

    Phylogenomic databases

    eggNOGi COG0696.
    HOGENOMi HOG000223664.
    KOi K15633.
    OMAi DACIGRV.
    OrthoDBi EOG6HJ22X.
    PhylomeDBi P37689.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00186 .
    BioCyci EcoCyc:PGMI-MONOMER.
    ECOL316407:JW3587-MONOMER.
    MetaCyc:PGMI-MONOMER.
    SABIO-RK P37689.

    Miscellaneous databases

    PROi P37689.

    Gene expression databases

    Genevestigatori P37689.

    Family and domain databases

    Gene3Di 3.40.1450.10. 1 hit.
    3.40.720.10. 2 hits.
    HAMAPi MF_01038. GpmI.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR011258. BPG-indep_PGM_N.
    IPR006124. Metalloenzyme.
    IPR005995. Pgm_bpd_ind.
    [Graphical view ]
    Pfami PF06415. iPGM_N. 1 hit.
    PF01676. Metalloenzyme. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001492. IPGAM. 1 hit.
    SUPFAMi SSF53649. SSF53649. 2 hits.
    SSF64158. SSF64158. 1 hit.
    TIGRFAMsi TIGR01307. pgm_bpd_ind. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
      Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
      Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography."
      Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.
      Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: B / BL21.
    5. "The two analogous phosphoglycerate mutases of Escherichia coli."
      Fraser H.I., Kvaratskhelia M., White M.F.
      FEBS Lett. 455:344-348(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiGPMI_ECOLI
    AccessioniPrimary (citable) accession number: P37689
    Secondary accession number(s): Q2M7S6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3