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P37689 (GPMI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2,3-bisphosphoglycerate-independent phosphoglycerate mutase

Short name=BPG-independent PGAM
Short name=Phosphoglyceromutase
Short name=iPGM
EC=5.4.2.12
Gene names
Name:gpmI
Synonyms:pgmI, yibO
Ordered Locus Names:b3612, JW3587
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate. HAMAP-Rule MF_01038

Catalytic activity

2-phospho-D-glycerate = 3-phospho-D-glycerate. HAMAP-Rule MF_01038

Cofactor

Binds 2 manganese ions per subunit.

Enzyme regulation

Insensitive to vanadate. HAMAP-Rule MF_01038

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5. HAMAP-Rule MF_01038

Subunit structure

Monomer.

Developmental stage

Expressed most strongly in early exponential growth, with levels falling off as cells reached late log phase and stationary phase. HAMAP-Rule MF_01038

Sequence similarities

Belongs to the BPG-independent phosphoglycerate mutase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5145142,3-bisphosphoglycerate-independent phosphoglycerate mutase HAMAP-Rule MF_01038
PRO_0000212145

Sites

Active site641Phosphoserine intermediate By similarity
Metal binding141Manganese 2 By similarity
Metal binding641Manganese 2 By similarity
Metal binding4031Manganese 1 By similarity
Metal binding4071Manganese 1 By similarity
Metal binding4441Manganese 2 By similarity
Metal binding4451Manganese 2 By similarity
Metal binding4631Manganese 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
P37689 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: BD4173C2BA2CEDD8

FASTA51456,194
        10         20         30         40         50         60 
MLVSKKPMVL VILDGYGYRE EQQDNAIFSA KTPVMDALWA NRPHTLIDAS GLEVGLPDRQ 

        70         80         90        100        110        120 
MGNSEVGHVN LGAGRIVYQD LTRLDVEIKD RAFFANPVLT GAVDKAKNAG KAVHIMGLLS 

       130        140        150        160        170        180 
AGGVHSHEDH IMAMVELAAE RGAEKIYLHA FLDGRDTPPR SAESSLKKFE EKFAALGKGR 

       190        200        210        220        230        240 
VASIIGRYYA MDRDNRWDRV EKAYDLLTLA QGEFQADTAV AGLQAAYARD ENDEFVKATV 

       250        260        270        280        290        300 
IRAEGQPDAA MEDGDALIFM NFRADRAREI TRAFVNADFD GFARKKVVNV DFVMLTEYAA 

       310        320        330        340        350        360 
DIKTAVAYPP ASLVNTFGEW MAKNDKTQLR ISETEKYAHV TFFFNGGVEE SFKGEDRILI 

       370        380        390        400        410        420 
NSPKVATYDL QPEMSSAELT EKLVAAIKSG KYDTIICNYP NGDMVGHTGV MEAAVKAVEA 

       430        440        450        460        470        480 
LDHCVEEVAK AVESVGGQLL ITADHGNAEQ MRDPATGQAH TAHTNLPVPL IYVGDKNVKA 

       490        500        510 
VEGGKLSDIA PTMLSLMGME IPQEMTGKPL FIVE 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography."
Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.
Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
Strain: B / BL21.
[5]"The two analogous phosphoglycerate mutases of Escherichia coli."
Fraser H.I., Kvaratskhelia M., White M.F.
FEBS Lett. 455:344-348(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00039 Genomic DNA. Translation: AAB18589.1.
U00096 Genomic DNA. Translation: AAC76636.1.
AP009048 Genomic DNA. Translation: BAE77680.1.
PIRS47833.
RefSeqNP_418069.1. NC_000913.3.
YP_491821.1. NC_007779.1.

3D structure databases

ProteinModelPortalP37689.
SMRP37689. Positions 5-504.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-12425N.
IntActP37689. 4 interactions.
STRING511145.b3612.

2D gel databases

SWISS-2DPAGEP37689.

Proteomic databases

PaxDbP37689.
PRIDEP37689.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76636; AAC76636; b3612.
BAE77680; BAE77680; BAE77680.
GeneID12934300.
948130.
KEGGecj:Y75_p3562.
eco:b3612.
PATRIC32122711. VBIEscCol129921_3732.

Organism-specific databases

EchoBASEEB2204.
EcoGeneEG12296. gpmI.

Phylogenomic databases

eggNOGCOG0696.
HOGENOMHOG000223664.
KOK15633.
OMADACIGRV.
OrthoDBEOG6HJ22X.
PhylomeDBP37689.

Enzyme and pathway databases

BioCycEcoCyc:PGMI-MONOMER.
ECOL316407:JW3587-MONOMER.
MetaCyc:PGMI-MONOMER.
SABIO-RKP37689.
UniPathwayUPA00109; UER00186.

Gene expression databases

GenevestigatorP37689.

Family and domain databases

Gene3D3.40.1450.10. 1 hit.
3.40.720.10. 2 hits.
HAMAPMF_01038. GpmI.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR011258. BPG-indep_PGM_N.
IPR006124. Metalloenzyme.
IPR005995. Pgm_bpd_ind.
[Graphical view]
PfamPF06415. iPGM_N. 1 hit.
PF01676. Metalloenzyme. 1 hit.
[Graphical view]
PIRSFPIRSF001492. IPGAM. 1 hit.
SUPFAMSSF53649. SSF53649. 2 hits.
SSF64158. SSF64158. 1 hit.
TIGRFAMsTIGR01307. pgm_bpd_ind. 1 hit.
ProtoNetSearch...

Other

PROP37689.

Entry information

Entry nameGPMI_ECOLI
AccessionPrimary (citable) accession number: P37689
Secondary accession number(s): Q2M7S6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene