Reviewed, UniProtKB/Swiss-Prot P37686 (ADH2_ECOLI)

Names and origin

Protein names

Recommended name:
    Probable alcohol dehydrogenase
    EC=1.1.1.1

Gene names

Name:	yiaY
Ordered Locus Names:	b3589, JW5648

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Iron By similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	Iron NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Probable alcohol dehydrogenase		PRO_0000087816
Experimental info
	Sequence conflict	380 – 383	4	RAAM → AAR in AAB18566. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P37686-1 [UniParc]. Last modified October 11, 2004. Version 4. Checksum: 805DC4E488E108F6	FASTA	383	40,351
	10 20 30 40 50 60 MAASTFFIPS VNVIGADSLT DAMNMMADYG FTRTLIVTDN MLTKLGMAGD VQKALEERNI 70 80 90 100 110 120 FSVIYDGTQP NPTTENVAAG LKLLKENNCD SVISLGGGSP HDCAKGIALV AANGGDIRDY 130 140 150 160 170 180 EGVDRSAKPQ LPMIAINTTA GTASEMTRFC IITDEARHIK MAIVDKHVTP LLSVNDSSLM 190 200 210 220 230 240 IGMPKSLTAA TGMDALTHAI EAYVSIAATP ITDACALKAV TMIAENLPLA VEDGSNAKAR 250 260 270 280 290 300 EAMAYAQFLA GMAFNNASLG YVHAMAHQLG GFYNLPHGVC NAVLLPHVQV FNSKVAAARL 310 320 330 340 350 360 RDCAAAMGVN VTGKNDAEGA EACINAIREL AKKVDIPAGL RDLNVKEEDF AVLATNALKD 370 380 ACGFTNPIQA THEEIVAIYR AAM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed: 16397293] [Abstract] Cited for: SEQUENCE REVISION TO 380-383.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"aldB, an RpoS-dependent gene in Escherichia coli encoding an aldehyde dehydrogenase that is repressed by Fis and activated by Crp." Xu J., Johnson R.C. J. Bacteriol. 177:3166-3175(1995) [PubMed: 7768815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 336-383. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00039 Genomic DNA. Translation: AAB18566.1. U00096 Genomic DNA. Translation: AAT48195.1. AP009048 Genomic DNA. Translation: BAE77704.1. L40742 Genomic DNA. Translation: AAC36938.1.
PIR	S47810.
RefSeq	AP_004203.1. YP_026233.1.
3D structure databases
SMR	P37686. Positions 4-383.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12414N.
STRING	P37686.
Genome annotation databases
GeneID	948102.
GenomeReviews	Gene locus JW5648 in contig AP009048_GR. Gene locus b3589 in contig U00096_GR.
KEGG	ecj:JW5648. eco:b3589.
Organism-specific databases
EchoBASE	EB2201.
EcoGene	EG12293. yiaY.
CMR	Search...
Phylogenomic databases
eggNOG	COG1454.
HOGENOM	HBG742415.
OMA	ARDQMAY.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12293-MONOMER.
Gene expression databases
Genevestigator	P37686.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADH2_ECOLI

Accession

Primary (citable) accession number: P37686
Secondary accession number(s): Q2M7Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 11, 2004
Last modified:	January 19, 2010
This is version 66 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)