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Protein

Aldehyde dehydrogenase B

Gene

aldB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NADP-dependent oxidation of diverse aldehydes such as chloroacetaldehyde, acetaldehyde, propionaldehyde, benzaldehyde, mafosfamide, 4-hydroperoxycyclophosphamide. Its preferred substrates are acetaldehyde and chloroacetaldehyde.

Miscellaneous

Does not use either glyceraldehyde or glycolaldehyde as substrates.

Cofactori

Enzyme regulationi

Negatively regulated by fis. Positively regulated by RpoS and crp. Induced by ethanol. Expression is maximally induced during the transition from exponential phase to stationary phase.

Kineticsi

the highest catalytic efficiency was observed with acetaldehyde.
  1. KM=2.5 µM for acetaldehyde
  2. KM=3.6 µM for chloroacetaldehyde
  3. KM=5.8 µM for propionaldehyde
  4. KM=56.8 µM for benzaldehyde
  5. KM=900 µM for 4-hydroperoxycyclophosphamide
  1. Vmax=2 µmol/min/mg enzyme with acetaldehyde as substrate
  2. Vmax=3.3 µmol/min/mg enzyme with chloroacetaldehyde as substrate
  3. Vmax=1 µmol/min/mg enzyme with propionaldehyde as substrate
  4. Vmax=0.6 µmol/min/mg enzyme with benzaldehyde as substrate
  5. Vmax=0.2 µmol/min/mg enzyme with 4-hydroperoxycyclophosphamide

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei268By similarity1
Active sitei307By similarity1

GO - Molecular functioni

  • aldehyde dehydrogenase (NAD) activity Source: EcoliWiki
  • oxidoreductase activity Source: EcoliWiki

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • response to ethanol Source: EcoliWiki

Keywordsi

Molecular functionOxidoreductase
LigandMagnesium, NADP

Enzyme and pathway databases

BioCyciEcoCyc:ALDDEHYDROGB-MONOMER
MetaCyc:ALDDEHYDROGB-MONOMER
SABIO-RKiP37685

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde dehydrogenase B (EC:1.2.1.-)
Gene namesi
Name:aldB
Synonyms:yiaX
Ordered Locus Names:b3588, JW3561
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12292 aldB

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi197R → E: Less than 10% of wild-type acetaldehyde dehydrogenase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000565651 – 512Aldehyde dehydrogenase BAdd BLAST512

Proteomic databases

EPDiP37685
PaxDbiP37685
PRIDEiP37685

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi4262555, 18 interactors
DIPiDIP-9082N
IntActiP37685, 3 interactors
MINTiP37685
STRINGi316385.ECDH10B_3769

Structurei

3D structure databases

ProteinModelPortaliP37685
SMRiP37685
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105C26 Bacteria
COG1012 LUCA
HOGENOMiHOG000271505
InParanoidiP37685
KOiK00138
OMAiTFVQEDV
PhylomeDBiP37685

Family and domain databases

Gene3Di3.40.309.10, 1 hit
3.40.605.10, 2 hits
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016160 Ald_DH_CS_CYS
IPR029510 Ald_DH_CS_GLU
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
PfamiView protein in Pfam
PF00171 Aldedh, 1 hit
SUPFAMiSSF53720 SSF53720, 1 hit
PROSITEiView protein in PROSITE
PS00070 ALDEHYDE_DEHYDR_CYS, 1 hit
PS00687 ALDEHYDE_DEHYDR_GLU, 1 hit

Sequencei

Sequence statusi: Complete.

P37685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNNPPSAQI KPGEYGFPLK LKARYDNFIG GEWVAPADGE YYQNLTPVTG
60 70 80 90 100
QLLCEVASSG KRDIDLALDA AHKVKDKWAH TSVQDRAAIL FKIADRMEQN
110 120 130 140 150
LELLATAETW DNGKPIRETS AADVPLAIDH FRYFASCIRA QEGGISEVDS
160 170 180 190 200
ETVAYHFHEP LGVVGQIIPW NFPLLMASWK MAPALAAGNC VVLKPARLTP
210 220 230 240 250
LSVLLLMEIV GDLLPPGVVN VVNGAGGVIG EYLATSKRIA KVAFTGSTEV
260 270 280 290 300
GQQIMQYATQ NIIPVTLELG GKSPNIFFAD VMDEEDAFFD KALEGFALFA
310 320 330 340 350
FNQGEVCTCP SRALVQESIY ERFMERAIRR VESIRSGNPL DSVTQMGAQV
360 370 380 390 400
SHGQLETILN YIDIGKKEGA DVLTGGRRKL LEGELKDGYY LEPTILFGQN
410 420 430 440 450
NMRVFQEEIF GPVLAVTTFK TMEEALELAN DTQYGLGAGV WSRNGNLAYK
460 470 480 490 500
MGRGIQAGRV WTNCYHAYPA HAAFGGYKQS GIGRETHKMM LEHYQQTKCL
510
LVSYSDKPLG LF
Length:512
Mass (Da):56,306
Last modified:November 1, 1995 - v2
Checksum:iE673C34340DF68CD
GO

Sequence cautioni

The sequence AAB18565 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Mass spectrometryi

Molecular mass is 56352 Da from positions 1 - 512. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40742 Genomic DNA Translation: AAC36939.1
U00039 Genomic DNA Translation: AAB18565.1 Different initiation.
U00096 Genomic DNA Translation: AAC76612.2
AP009048 Genomic DNA Translation: BAE77705.1
PIRiS47809
RefSeqiNP_418045.4, NC_000913.3
WP_000183980.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76612; AAC76612; b3588
BAE77705; BAE77705; BAE77705
GeneIDi948104
KEGGiecj:JW3561
eco:b3588
PATRICifig|1411691.4.peg.3123

Similar proteinsi

Entry informationi

Entry nameiALDB_ECOLI
AccessioniPrimary (citable) accession number: P37685
Secondary accession number(s): P78118, Q2M7Q1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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