Reviewed, UniProtKB/Swiss-Prot P37685 (ALDB_ECOLI)

Names and origin

Protein names

Recommended name:
    Aldehyde dehydrogenase B
    EC=1.2.1.-

Gene names

Name:	aldB
Synonyms:	yiaX
Ordered Locus Names:	b3588, JW3561

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the NADP-dependent oxidation of diverse aldehydes such as chloroacetaldehyde, acetaldehyde, propionaldehyde, benzaldehyde, mafosfamide, 4-hydroperoxycyclophosphamide. Its preferred substrates are acetaldehyde and chloroacetaldehyde.
Cofactor	Magnesium.
Enzyme regulation	Negatively regulated by fis. Positively regulated by rpoS and crp. Induced by ethanol. Expression is maximally induced during the transition from exponential phase to stationary phase.
Subunit structure	Homotetramer.
Miscellaneous	Does not use either glyceraldehyde or glycolaldehyde as substrates.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.
biophysicochemical properties	Kinetic parameters: the highest catalytic efficiency was observed with acetaldehyde. KM=2.5 µM for acetaldehyde KM=3.6 µM for chloroacetaldehyde KM=5.8 µM for propionaldehyde KM=56.8 µM for benzaldehyde KM=900 µM for 4-hydroperoxycyclophosphamide Vmax=2 µmol/min/mg enzyme with acetaldehyde as substrate Vmax=3.3 µmol/min/mg enzyme with chloroacetaldehyde as substrate Vmax=1 µmol/min/mg enzyme with propionaldehyde as substrate Vmax=0.6 µmol/min/mg enzyme with benzaldehyde as substrate Vmax=0.2 µmol/min/mg enzyme with 4-hydroperoxycyclophosphamide
Mass spectrometry	Molecular mass is 56352 Da from positions 1 - 512. Determined by MALDI. Ref.5

Ontologies

Keywords
Ligand	Magnesium NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dnaK	P0A6Y8	1	EBI-1132062,EBI-542092

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Aldehyde dehydrogenase B		PRO_0000056565
Sites
	Active site	268	1	By similarity
	Active site	307	1	By similarity
Experimental info
	Mutagenesis	197	1	R → E: Less than 10% of wild-type acetaldehyde dehydrogenase activity. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P37685-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: E673C34340DF68CD	FASTA	512	56,306
	10 20 30 40 50 60 MTNNPPSAQI KPGEYGFPLK LKARYDNFIG GEWVAPADGE YYQNLTPVTG QLLCEVASSG 70 80 90 100 110 120 KRDIDLALDA AHKVKDKWAH TSVQDRAAIL FKIADRMEQN LELLATAETW DNGKPIRETS 130 140 150 160 170 180 AADVPLAIDH FRYFASCIRA QEGGISEVDS ETVAYHFHEP LGVVGQIIPW NFPLLMASWK 190 200 210 220 230 240 MAPALAAGNC VVLKPARLTP LSVLLLMEIV GDLLPPGVVN VVNGAGGVIG EYLATSKRIA 250 260 270 280 290 300 KVAFTGSTEV GQQIMQYATQ NIIPVTLELG GKSPNIFFAD VMDEEDAFFD KALEGFALFA 310 320 330 340 350 360 FNQGEVCTCP SRALVQESIY ERFMERAIRR VESIRSGNPL DSVTQMGAQV SHGQLETILN 370 380 390 400 410 420 YIDIGKKEGA DVLTGGRRKL LEGELKDGYY LEPTILFGQN NMRVFQEEIF GPVLAVTTFK 430 440 450 460 470 480 TMEEALELAN DTQYGLGAGV WSRNGNLAYK MGRGIQAGRV WTNCYHAYPA HAAFGGYKQS 490 500 510 GIGRETHKMM LEHYQQTKCL LVSYSDKPLG LF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"aldB, an RpoS-dependent gene in Escherichia coli encoding an aldehyde dehydrogenase that is repressed by Fis and activated by Crp." Xu J., Johnson R.C. J. Bacteriol. 177:3166-3175(1995) [PubMed: 7768815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TRANSCRIPTION REGULATION. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Isolation and characterization of an aldehyde dehydrogenase encoded by the aldB gene of Escherichia coli." Ho K.K., Weiner H. J. Bacteriol. 187:1067-1073(2005) [PubMed: 15659684] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION, MASS SPECTROMETRY, MUTAGENESIS OF ARG-197. Strain: K12 / DH5-alpha.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L40742 Genomic DNA. Translation: AAC36939.1. U00039 Genomic DNA. Translation: AAB18565.1. Different initiation. U00096 Genomic DNA. Translation: AAC76612.1. Different initiation. AP009048 Genomic DNA. Translation: BAE77705.1.
PIR	S47809.
RefSeq	AP_004204.1. NP_418045.4.
3D structure databases
HSSP	HSSP built from PDB template 1O04 based on UniProtKB P05091.
ModBase	Search...
Protein-protein interaction databases
IntAct	P37685. 2 interactions.
Genome annotation databases
GeneID	948104.
GenomeReviews	Gene locus JW3561 in contig AP009048_GR. Gene locus b3588 in contig U00096_GR.
KEGG	ecj:JW3561. eco:b3588.
Organism-specific databases
EchoBASE	EB2200.
EcoGene	EG12292. aldB.
CMR	Search...
Phylogenomic databases
HOGENOM	P37685.
OMA	P37685. INGQPFC.
Enzyme and pathway databases
BioCyc	EcoCyc:ALDDEHYDROGB-MON. MetaCyc:ALDDEHYDROGB-MON.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALDB_ECOLI

Accession

Primary (citable) accession number: P37685
Secondary accession number(s): P78118, Q2M7Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)