P37680 (SGBE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-ribulose-5-phosphate 4-epimerase SgbE
EC=5.1.3.4

Alternative name(s):
Phosphoribulose isomerase

Gene names

Name:	sgbE
Synonyms:	yiaS
Ordered Locus Names:	b3583, JW3555

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. May be involved in the utilization of 2,3-diketo-L-gulonate. Ref.5 Ref.6
Catalytic activity	L-ribulose 5-phosphate = D-xylulose 5-phosphate.
Cofactor	Binds 1 zinc ion per subunit Potential.
Sequence similarities	Belongs to the aldolase class II family. AraD/FucA subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-arabinose catabolic process Inferred from electronic annotation. Source: InterPro L-lyxose metabolic process Inferred from mutant phenotype Ref.5. Source: EcoCyc
Molecular function	L-ribulose-phosphate 4-epimerase activity Inferred from direct assay Ref.5. Source: EcoCyc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	L-ribulose-5-phosphate 4-epimerase SgbE		PRO_0000162923
Sites
	Metal binding	76	1	Zinc By similarity
	Metal binding	95	1	Zinc By similarity
	Metal binding	97	1	Zinc By similarity
	Metal binding	171	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37680 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: F4FF4D7EC2A80B3A	FASTA	231	25,561
	10 20 30 40 50 60 MLEQLKADVL AANLALPAHH LVTFTWGNVS AVDETRQWMV IKPSGVEYDV MTADDMVVVE 70 80 90 100 110 120 IASGKVVEGS KKPSSDTPTH LALYRRYAEI GGIVHTHSRH ATIWSQAGLD LPAWGTTHAD 130 140 150 160 170 180 YFYGAIPCTR QMTAEEINGE YEYQTGEVII ETFEERGRSP AQIPAVLVHS HGPFAWGKNA 190 200 210 220 230 ADAVHNAVVL EECAYMGLFS RQLAPQLPAM QNELLDKHYL RKHGANAYYG Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Novel phosphotransferases system genes revealed by bacterial genome analysis: operons encoding homologues of sugar-specific permease domains of the phosphotransferase system and pentose catabolic enzymes." Reizer J., Charbit A., Reizer A., Saier M.H. Jr. Genome Sci. Technol. 1:53-75(1996) Cited for: DISCUSSION OF SEQUENCE.
[5]	"Role of the yiaR and yiaS genes of Escherichia coli in metabolism of endogenously formed L-xylulose." Ibanez E., Gimenez R., Pedraza T., Baldoma L., Aguilar J., Badia J. J. Bacteriol. 182:4625-4627(2000) [PubMed: 10913097] [Abstract] Cited for: FUNCTION.
[6]	"Utilization of L-ascorbate by Escherichia coli K-12: assignments of functions to products of the yjf-sga and yia-sgb operons." Yew W.S., Gerlt J.A. J. Bacteriol. 184:302-306(2002) [PubMed: 11741871] [Abstract] Cited for: FUNCTION, CHARACTERIZATION. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00039 Genomic DNA. Translation: AAB18560.1. U00096 Genomic DNA. Translation: AAC76607.1. AP009048 Genomic DNA. Translation: BAE77710.1.
PIR	S47804.
RefSeq	NP_418040.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P37680.
SMR	P37680. Positions 1-223.
ModBase	Search...
Protein-protein interaction databases
IntAct	P37680. 4 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002358; EBESCP00000002358; EBESCG00000001926. EBESCT00000002359; EBESCP00000002359; EBESCG00000001926. EBESCT00000014278; EBESCP00000013569; EBESCG00000013340.
GeneID	948099.
GenomeReviews	Gene locus JW3555 in contig AP009048_GR. Gene locus b3583 in contig U00096_GR.
KEGG	ecj:JW3555. eco:b3583.
PATRIC	32122642. VBIEscCol129921_3698.
Organism-specific databases
EchoBASE	EB2195.
EcoGene	EG12287. sgbE.
Phylogenomic databases
eggNOG	COG0235.
GeneTree	EBGT00050000009319.
HOGENOM	HBG541069.
OMA	ISCGAEN.
PhylomeDB	P37680.
ProtClustDB	PRK12347.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12287-MONOMER. MetaCyc:EG12287-MONOMER.
Gene expression databases
Genevestigator	P37680.
Family and domain databases
InterPro	IPR001303. Aldolase_II/adducin_N. IPR004661. AraD. [Graphical view]
Gene3D	G3DSA:3.40.225.10. Aldolase_II/adducin_N. 1 hit.
KO	K03080.
Pfam	PF00596. Aldolase_II. 1 hit. [Graphical view]
SMART	SM01007. Aldolase_II. 1 hit. [Graphical view]
SUPFAM	SSF53639. Aldolase_II_N. 1 hit.
TIGRFAMs	TIGR00760. AraD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SGBE_ECOLI

Accession

Primary (citable) accession number: P37680
Secondary accession number(s): Q2M7P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program